BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,365,836	T→C	42.9%	intergenic (‑216/‑92)	ais ← / → arnB	putative LPS core heptose(II)‑phosphate phosphatase/uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,365,836	0	T	C	42.9%	63.6 / 189.9	162	intergenic (‑216/‑92)	ais/arnB	putative LPS core heptose(II)‑phosphate phosphatase/uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base T (33/59); new base C (31/38); total (64/98)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 163 total aligned reads displayed.

AAAATTTTGCCAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAG  >  NC_000913/2365740‑2365934

      ttgccAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAAt                                                                                           <  2:112689‑M2/95‑1 (MQ=255)

       tgccAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAAtt                                                                                          >  2:3044412‑M2/5‑100 (MQ=255)

        gccAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAAttt                                                                                         >  2:3505826‑M2/4‑100 (MQ=255)

        gccAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAAttt                                                                                         >  2:2040569‑M2/4‑100 (MQ=255)

         ccAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTc                                                                                        >  1:804018‑M2/3‑100 (MQ=255)

         ccAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTc                                                                                        <  2:2027301‑M2/98‑1 (MQ=255)

         ccAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTc                                                                                        >  2:3496526‑M2/3‑100 (MQ=255)

          cAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTc                                                                                        <  1:751520‑M2/98‑1 (MQ=255)

           ataTGCAAAACCTAAATAAAAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGc                                                                                      <  1:2591135/98‑1 (MQ=255)

           aaaTGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGc                                                                                      <  2:3266625/100‑1 (MQ=255)

           aaaTGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGc                                                                                      <  2:2014014/100‑1 (MQ=255)

           aaaTGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGc                                                                                      <  2:708485/100‑1 (MQ=255)

            aaTGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCt                                                                                     <  1:1802909/100‑1 (MQ=255)

            aaTGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCt                                                                                     >  1:3936225/1‑100 (MQ=255)

               gCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCa                                                                                  >  2:411675/1‑100 (MQ=255)

                cAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCaa                                                                                 >  2:3474648/1‑100 (MQ=255)

                cAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCATTAAAATTTCGCTCCaa                                                                                 >  1:1538516/1‑100 (MQ=255)

                  aaGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAAt                                                                               >  2:1311159/1‑100 (MQ=255)

                   aGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCACATg                                                                              <  1:1175124/100‑1 (MQ=255)

                    gCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATg                                                                              >  1:3818785/1‑99 (MQ=255)

                    gCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATgg                                                                             <  1:3025773/100‑1 (MQ=255)

                      cTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATgg                                                                             <  1:1508788/98‑1 (MQ=255)

                      cTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATg                                                                              >  1:3285758/1‑97 (MQ=255)

                      cTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCa                                                                           <  2:1989341/100‑1 (MQ=255)

                       tAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCaa                                                                          <  2:4251885/100‑1 (MQ=255)

                       tAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCaa                                                                          <  2:1078801/100‑1 (MQ=255)

                        aaaTAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCaaa                                                                         >  1:181109/1‑100 (MQ=255)

                         aaTAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTCTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCaaaa                                                                        >  1:2367134/1‑100 (MQ=255)

                           tAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAata                                                                      <  2:3269077/100‑1 (MQ=255)

                            aaGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAatat                                                                     <  1:1100730/100‑1 (MQ=255)

                             aGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAAtata                                                                    <  2:2864245/100‑1 (MQ=255)

                             aGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGATTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAAtata                                                                    <  1:1418538/100‑1 (MQ=255)

                              gAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATac                                                                   >  1:2737574/1‑100 (MQ=255)

                               aaaaaaTATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATaca                                                                  >  1:3725003/1‑100 (MQ=255)

                                aaaaaTATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATacac                                                                 <  1:1503192/100‑1 (MQ=255)

                                  aaaTATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACacaa                                                               <  2:2049498/100‑1 (MQ=255)

                                   aaTATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACacaac                                                              <  1:1997055/100‑1 (MQ=255)

                                   aaTATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACacaac                                                              <  1:916955/100‑1 (MQ=255)

                                     tataAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAacac                                                            >  2:3737373/1‑100 (MQ=255)

                                       taAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACActc                                                          >  2:1584458/1‑100 (MQ=255)

                                       taAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACActc                                                          >  1:2214377/1‑100 (MQ=255)

                                         aaaaTTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCtt                                                        <  1:2392445/100‑1 (MQ=255)

                                          aaaTTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAAGATACACAACACTCttt                                                       <  1:1329301/100‑1 (MQ=255)

                                          aaaTTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCttt                                                       <  2:2586930/100‑1 (MQ=255)

                                            aTTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTtat                                                     <  2:311302/100‑1 (MQ=255)

                                              ttCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAg                                                   <  2:3024283/100‑1 (MQ=255)

                                              ttCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAg                                                   <  2:4272957/100‑1 (MQ=255)

                                               tCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGc                                                  >  2:132453/1‑100 (MQ=255)

                                               tCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGc                                                  <  1:3784415/100‑1 (MQ=255)

                                                cAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCa                                                 <  1:4099756/100‑1 (MQ=255)

                                                   tatTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAat                                              <  1:1716634/100‑1 (MQ=255)

                                                   tatTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAat                                              <  1:2680868/100‑1 (MQ=255)

                                                   tatTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAat                                              <  2:369312/100‑1 (MQ=255)

                                                     tttACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAatat                                            <  1:3539749/100‑1 (MQ=255)

                                                       tACGTCTAATATTAGTTTCTTAAGGTTAAGATAATATTCTACCCTTAAAATTTCGCTAGAAATGGCAAAAAATACACAACACGCTTTATAGCAAAtata                                           >  2:3726139/1‑99 (MQ=255)

                                                        aCGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAg                                         <  1:3637043/100‑1 (MQ=255)

                                                         cGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGt                                        <  2:887767/100‑1 (MQ=255)

                                                          gTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTg                                       <  1:942507/100‑1 (MQ=255)

                                                          gTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTg                                       >  2:3869877/1‑100 (MQ=255)

                                                          gTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCCTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAACAAATATaa                                          >  1:3758286/1‑97 (MQ=255)

                                                           tCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTgg                                      >  1:594189/1‑100 (MQ=255)

                                                               atatTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAg                                  <  1:1839652/100‑1 (MQ=255)

                                                                tatTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAgg                                 <  1:3044400/100‑1 (MQ=255)

                                                                tatTAGTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAgg                                 <  1:674837/100‑1 (MQ=255)

                                                                  ttAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTa                               <  2:1321361/100‑1 (MQ=255)

                                                                  ttAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTa                               <  2:2120784/100‑1 (MQ=255)

                                                                   tAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTAt                              <  1:424321/100‑1 (MQ=255)

                                                                    aGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTAtt                             >  2:1457663/1‑100 (MQ=255)

                                                                     gTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTc                            <  1:3972385/100‑1 (MQ=255)

                                                                     gTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTc                            >  1:2346081/1‑100 (MQ=255)

                                                                     gTTTCTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTc                            <  1:3117516/100‑1 (MQ=255)

                                                                      tttCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCa                           <  2:2296848/100‑1 (MQ=255)

                                                                         cTTAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCaa                          >  1:727660/1‑98 (MQ=255)

                                                                           tAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGc                      <  1:1714629/100‑1 (MQ=255)

                                                                           tAAGGTTAAGTTAATATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGc                      >  2:837117/1‑100 (MQ=255)

                                                                             aGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCgg                    <  1:696663/100‑1 (MQ=255)

                                                                               gttaagttaaTATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCggaa                  <  1:3147666/100‑1 (MQ=255)

                                                                               gttaagttaaTATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCggaa                  <  2:803206/100‑1 (MQ=255)

                                                                                ttaagttaaTATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCggaag                 >  1:2827979/1‑100 (MQ=255)

                                                                                ttaagttaaTATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCggaag                 <  2:3550986/100‑1 (MQ=255)

                                                                                 taagttaaTATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCggaagg                <  2:766141/100‑1 (MQ=255)

                                                                                 taagttaaTATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCggaagg                <  1:3193872/100‑1 (MQ=255)

                                                                                   agttaaTATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCggaaggaa              >  1:3025302/1‑100 (MQ=255)

                                                                                   agttaaTATTCTACCCTTAAAATTTCGCTCCAAAGGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCggaaggaa              <  1:506860/100‑1 (MQ=255)

                                                                                     ttaaTATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTCTCGCCAATCAAAGTGGACAGGTATTCAATGGCGGAAGGaaa             >  1:2501826/1‑99 (MQ=255)

                                                                                      taaTATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAg           >  2:2857234/1‑100 (MQ=255)

                                                                                      taaTATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAg           >  1:2106170/1‑100 (MQ=255)

                                                                                      taaTATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAg           <  1:1897521/100‑1 (MQ=255)

                                                                                       aaTATTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGc          <  2:3381887/100‑1 (MQ=255)

                                                                                        atatTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCa         <  1:3876350/100‑1 (MQ=255)

                                                                                         tatTCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCaa        >  2:855269/1‑100 (MQ=255)

                                                                                          atTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAAt       >  1:1850890/1‑100 (MQ=255)

                                                                                           ttCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATg      <  2:924567/100‑1 (MQ=255)

                                                                                           ttCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATg      <  2:1772023/100‑1 (MQ=255)

                                                                                           ttCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATg      >  2:2743766/1‑100 (MQ=255)

                                                                                           ttCTACCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATg      >  2:2538726/1‑100 (MQ=255)

                                                                                            tCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGt     <  2:2967615/100‑1 (MQ=255)

                                                                                              tATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCa   <  2:4120988/100‑1 (MQ=255)

                                                                                               aTCATTAAAATTTCGCTCCAAATGGCAAAATATACACTACACACTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAg  <  1:3012198/100‑1 (MQ=255)

                                                                                                tCCTTAAAATTTCGCGCCAAATGGCAAAATATACACGACACTCTTTATAGCAAAGAAAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAg  <  1:1125878/99‑1 (MQ=255)

AAAATTTTGCCAAATGCAAAGCCTAAATAAGAAAAAATATAAAAATTTCAATATTTACGTCTAATATTAGTTTCTTAAGGTTAAGTTAATATTCTATCCTTAAAATTTCGCTCCAAATGGCAAAATATACACAACACTCTTTATAGCAAATATAAGTGGACAGGTATTCAATGGCGGAAGGAAAAGCAATGTCAG  >  NC_000913/2365740‑2365934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`