Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,474,878 T→C 15.8% intergenic (+22/‑106) yfdQ → / → yfdR CPS‑53 (KpLE1) prophage; uncharacterized protein/CPS‑53 (KpLE1) prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,474,8780TC15.8% 445.0 / 73.9 197intergenic (+22/‑106)yfdQ/yfdRCPS‑53 (KpLE1) prophage; uncharacterized protein/CPS‑53 (KpLE1) prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base T (94/72);  new base C (27/4);  total (121/76)
Fisher's exact test for biased strand distribution p-value = 1.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01

Only 100 of 201 total aligned reads displayed.

ACGAATTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAG  >  NC_000913/2474780‑2474977
                                                                                                  |                                                                                                   
   aaTTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCgg                                                                                                 >  2:1605980/1‑100 (MQ=255)
   aaTTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCgg                                                                                                 <  2:3330165/100‑1 (MQ=255)
   aaTTTCGTGATCTGCTTGTTGAGAAAGTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCgg                                                                                                 <  1:215431/100‑1 (MQ=255)
    aTTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCggg                                                                                                <  1:3099603/100‑1 (MQ=255)
    aTTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCggg                                                                                                >  2:2097795/1‑100 (MQ=255)
      ttCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCggggg                                                                                              <  1:602494/100‑1 (MQ=255)
       tCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGc                                                                                             >  1:4103744/1‑100 (MQ=255)
        cGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGc                                                                                             <  1:228122/99‑1 (MQ=255)
        cGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCa                                                                                            >  1:78295/1‑100 (MQ=255)
         gTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCAt                                                                                           >  2:2309790/1‑100 (MQ=255)
         gTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCAt                                                                                           >  1:3059722/1‑100 (MQ=255)
           gATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCAttt                                                                                         >  1:1555997/1‑100 (MQ=255)
           gATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAttt                                                                                         <  1:1882650/100‑1 (MQ=255)
            aTCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAtttg                                                                                        <  2:4263737/100‑2 (MQ=255)
               tGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATgg                                                                                     >  1:2952998/1‑100 (MQ=255)
               tGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATgg                                                                                     <  1:2507973/100‑1 (MQ=255)
                  ttgttgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGaaa                                                                                  <  2:3632566/100‑1 (MQ=255)
                  tctttgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGaaa                                                                                  <  1:2589756/97‑1 (MQ=255)
                    gttgAGAAATTCAAAGTCAGCAAAGTAGAAAGCTTTATTGGTACTTTCACTGCCTGTTTTCATAACTGCAAATTCCCCTGCGGGGGAATTTAtttaaatg                                                                                >  1:3809431/1‑93 (MQ=255)
                    gttgAGAAATTCAAAGACAGCAAAGTAGATACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACg                                                                                <  2:2618304/100‑1 (MQ=255)
                    gttgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACg                                                                                >  2:2179849/1‑100 (MQ=255)
                    gttgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACg                                                                                >  1:2046297/1‑100 (MQ=255)
                     ttgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGaaaaga                                                                               >  2:2589764/1‑97 (MQ=255)
                      tgcgaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTACGGGGGCATTTATGGAAACGTa                                                                              <  1:1523417/97‑1 (MQ=255)
                      tgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTa                                                                              <  2:2455538/100‑1 (MQ=255)
                      tcagacagacAAAGACAGCAAAGTAGAAACCTTTATTGGCACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGaaa                                                                                  <  1:2728285/87‑1 (MQ=255)
                       gagaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTaa                                                                             >  2:1969867/1‑100 (MQ=255)
                       gagaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTaa                                                                             >  2:548516/1‑100 (MQ=255)
                       gagaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATCGAAACGTaa                                                                             >  2:2832199/1‑100 (MQ=255)
                        agaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAAt                                                                            >  2:445432/1‑100 (MQ=255)
                         gaAATTCAAAGACAGCAAAGTAGAAACCTTTAATGGTACTTTCACAGCCTGATTTCATTACTGCAATTGCCCCTGCGGGGGCATTTATGGACACGTAAtt                                                                           <  1:1421284/100‑1 (MQ=255)
                             ttCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACt                                                                       >  2:2282410/1‑100 (MQ=255)
                             ttCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACt                                                                       <  2:2953010/100‑1 (MQ=255)
                             ttCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACt                                                                       >  2:3773251/1‑100 (MQ=255)
                             ttCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACt                                                                       >  2:4012852/1‑100 (MQ=255)
                             ttCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACt                                                                       <  1:171585/100‑1 (MQ=255)
                              tCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTc                                                                      <  2:3087837/100‑1 (MQ=255)
                              tCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTc                                                                      >  1:2847189/1‑100 (MQ=255)
                              tCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTCCAAATGCCCCTGCGGGGGCATTTATGGATACGTAATTAAcgc                                                                      <  2:2892261/100‑3 (MQ=255)
                               cAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCa                                                                     <  1:752169/100‑1 (MQ=255)
                               cAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCa                                                                     >  2:1714100/1‑100 (MQ=255)
                                aaaGACAGCAAAGTAGACACCTTTATTGGTACTTTCACCGCCTGATGTCATTACTGCAAATGCCGCTGCGGCGGCATTTATGGAAAGGTAATTAACTc                                                                      >  1:2365370/1‑98 (MQ=255)
                                aaaGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCaa                                                                    >  2:180628/1‑100 (MQ=255)
                                 aaGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCaat                                                                   <  1:3363157/100‑1 (MQ=255)
                                   gACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCaataa                                                                 <  2:1424751/100‑1 (MQ=255)
                                       gCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCAc                                                             >  1:3212292/1‑100 (MQ=255)
                                       gCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCAc                                                             >  2:3584751/1‑100 (MQ=255)
                                       gCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCAc                                                             >  2:4197345/1‑100 (MQ=255)
                                        cAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCAcc                                                            <  2:1922533/100‑1 (MQ=255)
                                         aaaGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCg                                                           >  2:1780458/1‑100 (MQ=255)
                                              aGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTCATTAACTCAATAATCACCGGATgg                                                      >  1:1990858/1‑100 (MQ=255)
                                              aGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATgg                                                      >  1:3247404/1‑100 (MQ=255)
                                               gAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGt                                                     >  1:4029305/1‑100 (MQ=255)
                                                aaaCCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTg                                                    >  1:255877/1‑100 (MQ=255)
                                                    cTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAggg                                                <  1:1166934/100‑1 (MQ=255)
                                                      ttATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCt                                              <  2:3024812/100‑1 (MQ=255)
                                                           ggTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGTCTTTTATGGAAACGTAAGTAACGCAATAATCTCCGGATGGTTATGGCTTCCtt                                         >  1:1847967/1‑100 (MQ=255)
                                                           ggTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCtt                                         >  2:1071381/1‑100 (MQ=255)
                                                           ggTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCtt                                         >  1:2053339/1‑100 (MQ=255)
                                                            gTACTTTCCGTGCCTGATATCATTACTGGAAATGCCCCTGCGGGGGCATTTATGAAAACGTAATTAACGCAATAATCACCCGATGGTGAGAGCTTCCgtt                                        <  1:1222043/100‑1 (MQ=255)
                                                             tACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTACTTAACTCAATAATCCCCGGATGGTGAGGGCTTCCtttt                                       <  1:2643755/100‑1 (MQ=255)
                                                               cTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAc                                     <  2:1281541/100‑1 (MQ=255)
                                                               cTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAc                                     <  1:3950764/100‑1 (MQ=255)
                                                               cTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAc                                     <  1:2417530/100‑1 (MQ=255)
                                                               cTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAc                                     >  2:3218372/1‑100 (MQ=255)
                                                                tttCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAcc                                    <  2:1185658/100‑1 (MQ=255)
                                                                 ttCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAccc                                   >  1:3290699/1‑100 (MQ=255)
                                                                     ccGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAAc                               <  2:634455/100‑1 (MQ=255)
                                                                      cGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAAc                               <  1:2002847/99‑1 (MQ=255)
                                                                      cGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACt                              <  1:3788646/100‑1 (MQ=255)
                                                                          tGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAgc                          >  2:844397/1‑100 (MQ=255)
                                                                          tGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAgc                          <  1:515071/100‑1 (MQ=255)
                                                                              tgcATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAAGGCTTCCTTTTACCCAAACTCAGCGCgg                      >  2:3962019/3‑100 (MQ=255)
                                                                                cATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTg                    <  1:2818153/100‑1 (MQ=255)
                                                                                 aTTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGc                   >  1:218710/1‑100 (MQ=255)
                                                                                   tACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAg                 <  2:916266/100‑1 (MQ=255)
                                                                                   tACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAg                 <  2:1459748/100‑1 (MQ=255)
                                                                                     ccataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATTGTGAGAGCTTCCTTGTACCCAAACTCAGCGCGGTGCAgcg               >  1:4262371/5‑100 (MQ=38)
                                                                                     cTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg               >  1:3891091/1‑100 (MQ=255)
                                                                                     cTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg               <  2:4129416/100‑1 (MQ=255)
                                                                                     cTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGGAATTAACTCAATAATCACCGGATGGTGGGGGCTTCCTTTTAACCAAACGCAGCGCGGTGCAgc                >  1:681795/1‑99 (MQ=255)
                                                                                      cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  1:3677252/4‑100 (MQ=255)
                                                                                      cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:1680050/4‑100 (MQ=255)
                                                                                      cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:510684/4‑100 (MQ=255)
                                                                                      cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:1383910/4‑100 (MQ=255)
                                                                                      cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  1:1015527/4‑100 (MQ=255)
                                                                                      cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:4246701/4‑100 (MQ=255)
                                                                                      cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:2982142/4‑100 (MQ=255)
                                                                                       ataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGCTGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa             >  1:705063/3‑100 (MQ=255)
                                                                                       ataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa             >  2:2753693/3‑100 (MQ=255)
                                                                                       ataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa             >  2:764217/3‑100 (MQ=255)
                                                                                          aaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCatat          >  1:2190228/1‑100 (MQ=255)
                                                                                            tGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATAc        >  2:1643404/1‑100 (MQ=255)
                                                                                              ccccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACTCAAACTCAGCGCGGTGCAGCGCATATACg       >  1:2908811/1‑99 (MQ=255)
                                                                                               cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg     >  2:3872519/1‑100 (MQ=255)
                                                                                               cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg     <  1:484553/100‑1 (MQ=255)
                                                                                               cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg     >  2:1719780/1‑100 (MQ=255)
                                                                                                ccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTgg    >  1:692865/1‑100 (MQ=255)
                                                                                                ccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTgg    <  2:473057/100‑1 (MQ=255)
                                                                                                  tGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGgag  >  2:75244/1‑100 (MQ=255)
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ACGAATTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAG  >  NC_000913/2474780‑2474977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: