Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 358,599 G→A 45.5% A31V (GCG→GTG)  cynR ← transcriptional activator of cyn operon; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913358,5990GA45.5% 62.7 / 224.1 180A31V (GCG→GTG) cynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (47/51);  new base A (40/42);  total (87/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 184 total aligned reads displayed.

AGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCAACC  >  NC_000913/358501‑358698
                                                                                                  |                                                                                                   
aGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAgcgc                                                                                                    <  1:2338799/100‑1 (MQ=255)
 gACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCa                                                                                                   >  1:147152/1‑100 (MQ=255)
  aCTTCTCCTGCATCAGTGAGCCGAATCGTTCGCCCGCTACGGTCAACCAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAg                                                                                                  <  1:1988546/100‑1 (MQ=255)
   cTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAgg                                                                                                 <  2:2410316/100‑1 (MQ=255)
      ctcCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTg                                                                                              >  2:2129415/1‑100 (MQ=255)
      ctcCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTg                                                                                              <  2:688922/100‑1 (MQ=255)
      ctcCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTg                                                                                              <  1:2136592/100‑1 (MQ=255)
         cTGCATCAGTGAGCCGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGga                                                                                           <  2:1691867/100‑1 (MQ=255)
          tGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGgag                                                                                          >  1:2711538/1‑100 (MQ=255)
           gCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGgaga                                                                                         >  2:3311466/1‑100 (MQ=255)
             aTCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACg                                                                                       <  2:2514886/100‑1 (MQ=255)
             aTCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACg                                                                                       <  2:977680/100‑1 (MQ=255)
               naGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTg                                                                                     >  1:499073/2‑100 (MQ=255)
                aGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTg                                                                                     <  2:1010495/99‑1 (MQ=255)
                aGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGc                                                                                    >  2:1940241/1‑100 (MQ=255)
                aGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGc                                                                                    <  1:1604264/100‑1 (MQ=255)
                aGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGc                                                                                    <  2:2774373/100‑1 (MQ=255)
                  tGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCan                                                                                  <  2:4213736/100‑2 (MQ=255)
                   gagaCGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAAc                                                                                 >  2:4221115/1‑100 (MQ=255)
                     gaCGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGc                                                                               >  2:1544207/1‑100 (MQ=255)
                          aTCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTgg                                                                          <  1:3167553/100‑1 (MQ=255)
                           tCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTggc                                                                         <  2:2715915/100‑1 (MQ=255)
                            cGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACGCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcg                                                                        >  1:2624181/1‑100 (MQ=255)
                             gTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggctg                                                                       >  1:2596589/1‑98 (MQ=255)
                             gTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcgg                                                                       >  1:3629318/1‑100 (MQ=255)
                             gTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcgg                                                                       >  1:3468032/1‑100 (MQ=255)
                             gTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTggcgg                                                                       >  1:3432710/1‑100 (MQ=255)
                             gTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACCGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcgg                                                                       <  1:2219264/100‑1 (MQ=255)
                              ttCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTggcggc                                                                      >  1:788327/1‑100 (MQ=255)
                                cGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAACGTGCAGGATGGGAGACGTGCAACGCACTGGCGGCAc                                                                    >  2:713429/1‑100 (MQ=255)
                                 gCCCGCTACGGTCAACCAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACg                                                                   <  1:4276692/100‑1 (MQ=255)
                                 gCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACg                                                                   >  1:2863215/1‑100 (MQ=255)
                                 gCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACg                                                                   <  1:1742047/100‑1 (MQ=255)
                                  cccGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcagcttca                                                                  >  2:1066952/1‑93 (MQ=255)
                                  accGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTGTAACTGTCGACCCTGCTGGGAAAGCGCCGGTTGGCAGCCGTGCAACGCACTGGCGGCACg                                                                   >  1:3808498/2‑99 (MQ=255)
                                        aCGGTCAATCAGCGCCACGCCTAAACCCTCCTCTAGCTCGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACCCACTGGCGCCACggct                                                                <  1:2561262/96‑3 (MQ=255)
                                        aCGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAg                                                            >  2:220000/1‑100 (MQ=255)
                                        aCGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAg                                                            >  1:3967864/1‑100 (MQ=255)
                                        aCGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAg                                                            <  2:4152969/100‑1 (MQ=255)
                                         cGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGc                                                           >  1:3336250/1‑100 (MQ=255)
                                         cGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGAAGACGTGCAACGCACTGGCGGCACGGGTGAAGc                                                           >  1:1002520/1‑100 (MQ=255)
                                          ggTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCt                                                          >  1:4182405/1‑100 (MQ=255)
                                          ggTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCt                                                          >  2:2591526/1‑100 (MQ=255)
                                          ggTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCt                                                          >  2:2803094/1‑100 (MQ=255)
                                             cAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGcc                                                       >  2:341918/1‑100 (MQ=255)
                                             cAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGcc                                                       <  2:4083502/100‑1 (MQ=255)
                                              aaaCAGCGACACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCa                                                      <  1:69640/100‑1 (MQ=255)
                                               aaCAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCAt                                                     >  2:3950044/1‑100 (MQ=255)
                                                   gCGGCACGCCTAAACTCTCCTCTAACTGGCGCATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTc                                                 <  2:2688901/100‑1 (MQ=255)
                                                    cGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCa                                                >  2:3549333/1‑100 (MQ=255)
                                                      gCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGc                                              <  1:460222/100‑1 (MQ=255)
                                                       cACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGcc                                             >  2:382121/1‑100 (MQ=255)
                                                         cGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCAc                                           >  2:3030398/1‑100 (MQ=255)
                                                         cGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCAc                                           <  1:3030386/100‑1 (MQ=255)
                                                         cGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCAc                                           <  2:3040020/100‑1 (MQ=255)
                                                          gCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACg                                          >  2:1447201/1‑100 (MQ=255)
                                                          gCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACg                                          <  2:1521471/100‑1 (MQ=255)
                                                           ccTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACgg                                         <  2:2538518/100‑1 (MQ=255)
                                                            cTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGc                                        <  1:3329359/100‑1 (MQ=255)
                                                            cTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGc                                        <  2:1523587/100‑1 (MQ=255)
                                                             tAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCa                                       >  1:339432/1‑100 (MQ=255)
                                                             tAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCa                                       >  1:1097326/1‑100 (MQ=255)
                                                                aCTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGa                                    <  2:2643732/100‑1 (MQ=255)
                                                                aCTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGa                                    <  1:1926052/100‑1 (MQ=255)
                                                                  tctcCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGaaa                                  <  2:854229/100‑1 (MQ=255)
                                                                   ctcctcTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGaaaa                                 >  2:583903/1‑100 (MQ=255)
                                                                   ctcctcTAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGaaaa                                 <  1:1226037/100‑1 (MQ=255)
                                                                     cctcTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaata                               >  2:1972409/1‑100 (MQ=255)
                                                                      ctctAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaataa                              <  2:1364721/100‑1 (MQ=255)
                                                                      ctctAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaataa                              >  1:3976726/1‑100 (MQ=255)
                                                                      ctctAACTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaataa                              >  2:2661377/1‑100 (MQ=255)
                                                                       tctAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaataat                             <  2:1133444/100‑1 (MQ=255)
                                                                       tctAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAaataat                             <  2:2851022/100‑1 (MQ=255)
                                                                         tAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTg                           <  1:2575267/100‑1 (MQ=255)
                                                                            cttgctaaTCTTTTGGGAAAGCACCGGTTTGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGata                        <  2:3225136/94‑1 (MQ=255)
                                                                            cTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGata                        >  2:3867838/1‑100 (MQ=255)
                                                                            cTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGata                        <  1:1940237/100‑1 (MQ=255)
                                                                            cTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGata                        <  1:1806689/100‑1 (MQ=255)
                                                                            cTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGata                        >  2:2007175/1‑100 (MQ=255)
                                                                            cTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGata                        >  1:1386859/1‑100 (MQ=255)
                                                                            cTGGCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTACCATGTTCAGCCACGGCAAGAAAATAATTGata                        <  2:259842/100‑1 (MQ=255)
                                                                             tGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGat                         <  1:1586364/98‑1 (MQ=255)
                                                                              ggCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATg                      <  2:3462405/100‑1 (MQ=255)
                                                                              ggCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATg                      >  2:159037/1‑100 (MQ=255)
                                                                              ggCGAATCTGCTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATg                      >  2:3179209/1‑100 (MQ=255)
                                                                                cGAATCTGTTGGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTc                    >  1:3817412/1‑100 (MQ=255)
                                                                                 gAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCg                   <  2:2711548/100‑1 (MQ=255)
                                                                                      tgctgGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCgagaga              >  2:3658808/1‑100 (MQ=255)
                                                                                      tgctgGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCgagaga              >  1:3893618/1‑100 (MQ=255)
                                                                                      tgctgGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCgagaga              >  2:4115014/1‑100 (MQ=255)
                                                                                       gctgGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGagagag             >  1:1851049/1‑100 (MQ=255)
                                                                                        ctgGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGc            >  2:3081391/1‑100 (MQ=255)
                                                                                        ctgGGAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGc            >  1:1169305/1‑100 (MQ=255)
                                                                                         tgGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCa           <  2:3366212/100‑1 (MQ=255)
                                                                                          gggAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCAt          >  1:1305157/1‑100 (MQ=255)
                                                                                           ggAAAGCACAGGGTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCAtt         >  1:3144575/1‑100 (MQ=255)
                                                                                            gAAAGCACAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTc        >  2:2078315/1‑100 (MQ=255)
                                                                                             aaaGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCg       <  1:3198931/100‑1 (MQ=255)
                                                                                                  gcAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCAAcc  <  2:3629336/100‑1 (MQ=255)
                                                                                                  gcAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCAAcc  <  2:3418482/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
AGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCACGGGTGAAGCTGCCATGTTCAGCCACGGCAAGAAAATAATTGATATGTCGAGAGAGCATTCGCAACC  >  NC_000913/358501‑358698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: