Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,501,197 A→C 46.3% N23T (AAC→ACC)  ypdC → putative DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,501,1970AC46.3% 34.8 / 245.0 166N23T (AAC→ACC) ypdCputative DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (43/46);  new base C (42/35);  total (85/81)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 170 total aligned reads displayed.

GCCAGTTAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGTGCTGCG  >  NC_000913/2501098‑2501296
                                                                                                   |                                                                                                   
gCCAGTTAATGCATCTTTAATCGGGCACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTaa                                                                                                     >  2:3149782/1‑100 (MQ=255)
 ccAGTTAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAAc                                                                                                    <  2:626989/100‑1 (MQ=255)
 ccAGTTAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAAc                                                                                                    >  2:2094204/1‑100 (MQ=255)
   aGTTAATGCATCTTTAATCGGGCACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAAccc                                                                                                  >  1:2479593/1‑100 (MQ=255)
   aGTTAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAAccc                                                                                                  <  2:2084669/100‑1 (MQ=255)
      tAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCa                                                                                               <  2:3160257/100‑1 (MQ=255)
       aaTGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCATACCACGCaa                                                                                              >  1:1635936/1‑100 (MQ=255)
        aTGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAac                                                                                             >  2:2158200/1‑100 (MQ=255)
        aTGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAac                                                                                             <  1:3927298/100‑1 (MQ=255)
         tGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAact                                                                                            >  2:564057/1‑100 (MQ=255)
            aTCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAactact                                                                                         >  2:2918816/1‑100 (MQ=255)
             tCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTg                                                                                        >  2:617157/1‑100 (MQ=255)
              cTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTgg                                                                                       >  2:3341116/1‑100 (MQ=255)
              cTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTgg                                                                                       >  2:211725/1‑100 (MQ=255)
               tgtAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACGACTgg                                                                                       <  2:3009445/97‑1 (MQ=255)
                 tAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGgc                                                                                    <  2:1851822/100‑1 (MQ=255)
                  aaTCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGTCTATTTTTTCAGCGGCCTGGGGCTTACCCCGCAACTACTggg                                                                                      >  1:153817/1‑97 (MQ=255)
                  aaTCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGgcg                                                                                   <  1:800992/100‑1 (MQ=255)
                  aaTCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGgcg                                                                                   >  2:756051/1‑100 (MQ=255)
                   aTCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGgcgc                                                                                  >  2:3830935/1‑100 (MQ=255)
                   aTCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGgcgc                                                                                  <  1:2894508/100‑1 (MQ=255)
                   aTCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGgcgc                                                                                  >  1:3114695/1‑100 (MQ=255)
                    tCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGcgcg                                                                                 >  1:2470342/1‑100 (MQ=255)
                      gggAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTc                                                                               <  2:3961644/100‑1 (MQ=255)
                      gggAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTc                                                                               >  1:695398/1‑100 (MQ=255)
                      gggAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTc                                                                               <  2:3208414/100‑1 (MQ=255)
                        gAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTg                                                                             <  1:578883/100‑1 (MQ=255)
                          aCTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGt                                                                           <  2:1698631/100‑1 (MQ=255)
                           cTTTCATGAAAGCACCAGGCTTGCCCGCCGCTCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCGTACCCCGCAACTACTGGGGCGCGTCTGGtt                                                                          <  1:4261146/100‑1 (MQ=255)
                           cTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTTTTTGTTTTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGtt                                                                          >  1:2392993/1‑100 (MQ=255)
                           cTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGtt                                                                          >  1:3150727/1‑100 (MQ=255)
                           cTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGtt                                                                          >  1:985348/1‑100 (MQ=255)
                           cTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGtt                                                                          >  2:2835931/1‑100 (MQ=255)
                           cTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGACGCGTCTGGtt                                                                          >  1:1980829/1‑100 (MQ=255)
                             ttCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTg                                                                        >  1:1301728/1‑100 (MQ=255)
                              tCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTgc                                                                       <  2:4120562/100‑1 (MQ=255)
                                 tGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTgccag                                                                    >  1:1349569/1‑100 (MQ=255)
                                  gAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTgccagc                                                                   <  1:3326947/100‑1 (MQ=255)
                                  gAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTgccagc                                                                   >  1:2601046/1‑100 (MQ=255)
                                  gAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTgccagc                                                                   >  1:1895748/1‑100 (MQ=255)
                                    aaGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGCCTGGTTTgccagcca                                                                 >  2:4200273/1‑100 (MQ=255)
                                     aGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTgccagcca                                                                 >  1:116397/1‑99 (MQ=255)
                                      gCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCTACTACTGGGGCGCGTCTGGTTTGCcagccagc                                                               >  1:2362773/1‑100 (MQ=255)
                                       cACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCagccagcc                                                              <  1:3270966/100‑1 (MQ=255)
                                       cACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCagccagcc                                                              <  2:2588719/100‑1 (MQ=255)
                                       cACCAGGCTTGCCCGCCGATCAACAATTTGTCGCCGATCTGTTCAGCGGCCGTTTGCTTAACCCGCTACTACTGGGGCGCGTCTGTTTTGCCagccagcc                                                              <  1:2824001/100‑1 (MQ=255)
                                         ccAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcctg                                                            >  1:480487/1‑100 (MQ=255)
                                          cAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcctgc                                                           >  2:2386213/1‑100 (MQ=255)
                                           ttccgatctcgccgATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcctgcc                                                          <  2:4197068/91‑1 (MQ=255)
                                           aGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcctgcc                                                          <  2:21665/100‑1 (MQ=255)
                                           aGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcctgcc                                                          <  2:685461/100‑1 (MQ=255)
                                            ggCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCCACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcctgcct                                                         >  1:3445327/1‑100 (MQ=255)
                                            ggCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcctgcct                                                         >  2:2825229/1‑100 (MQ=255)
                                            ggCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcctgcct                                                         >  2:300607/1‑100 (MQ=255)
                                            ggCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAgcctgcct                                                         >  1:677961/1‑100 (MQ=255)
                                              cTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCg                                                       >  1:1757090/1‑100 (MQ=255)
                                               ttGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGt                                                      <  1:660246/100‑1 (MQ=255)
                                               ttGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGt                                                      <  1:153803/100‑1 (MQ=255)
                                                 gCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTg                                                    >  1:862214/1‑100 (MQ=255)
                                                  cccGCCGATCAACAATTTTTTTTTTTTTTTTTCAGCGGCCTGGGGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGc                                                   >  1:4265091/1‑100 (MQ=255)
                                                         aTCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTggg                                            <  2:2610948/100‑1 (MQ=255)
                                                         aTCAACAATGTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCCACGACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCTTCGTTGCCGGTgg                                             <  1:3681501/99‑1 (MQ=255)
                                                           caacaaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCa                                          >  2:849722/1‑100 (MQ=255)
                                                             acaaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGt                                        >  1:3019699/1‑100 (MQ=255)
                                                              caaTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGtt                                       >  1:2183544/1‑100 (MQ=255)
                                                                atgtttCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGttt                                      <  1:2954056/96‑1 (MQ=255)
                                                                aTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTa                                     <  2:1289127/100‑1 (MQ=255)
                                                                aTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGACAGCCTGCCTCGTTGCCGGTGGGCAGTTTa                                     >  1:3618212/1‑100 (MQ=255)
                                                                aTTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTa                                     <  1:989921/100‑1 (MQ=255)
                                                                 tttttCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTAt                                    <  2:3537880/100‑1 (MQ=255)
                                                                  ttttCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATg                                   <  2:2200090/100‑1 (MQ=255)
                                                                   tttCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGt                                  >  2:1890807/1‑100 (MQ=255)
                                                                   tttCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGt                                  >  2:1770054/1‑100 (MQ=255)
                                                                     tCGCCGATCTGTTAAGCGGCTTGGTGTTTACCTCGCAACTCCTGGGGCGCGTCTGGTTTGCTAGCGAGCCTGCCTCGTTGCCGGTGGGCAGTTTATg                                   <  1:3564162/97‑1 (MQ=255)
                                                                      cGCCGATCTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTAtt                               <  1:4026227/100‑1 (MQ=255)
                                                                          gATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGAtt                           <  2:1963103/100‑1 (MQ=255)
                                                                          gATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGAt                            <  2:3331111/99‑1 (MQ=255)
                                                                             cTGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTc                        <  1:1988637/100‑1 (MQ=255)
                                                                              tGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTcc                       >  1:2171519/1‑100 (MQ=255)
                                                                              tGTTCAGCGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTcc                       >  2:165470/1‑100 (MQ=255)
                                                                              tGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTcc                       <  2:1265185/100‑1 (MQ=255)
                                                                                 tCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCg                    <  2:801084/100‑1 (MQ=255)
                                                                                     cGGCCTGGTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTg                <  1:571666/100‑1 (MQ=255)
                                                                                     cGGCCTGGTGCTTAACCCGCAACGACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTg                <  1:1701886/100‑1 (MQ=255)
                                                                                     cGGCCTGCTGCTTGCCCCGCCACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCt                 <  1:3790953/99‑1 (MQ=255)
                                                                                      ggCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTgg               <  2:3829719/100‑1 (MQ=255)
                                                                                       gCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGa              <  2:920478/100‑1 (MQ=255)
                                                                                       gCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGa              >  1:1349178/1‑100 (MQ=255)
                                                                                        ccTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGat             <  2:1922350/100‑1 (MQ=255)
                                                                                          tGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGatat           <  2:3467245/100‑1 (MQ=255)
                                                                                          tGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGatat           >  2:2387356/1‑100 (MQ=255)
                                                                                           ggTGCTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATc          >  2:1161949/1‑100 (MQ=255)
                                                                                             tGCTTACCCCGCACCTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTTGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGt        <  1:4149189/100‑1 (MQ=255)
                                                                                             tGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGt        <  2:2002725/100‑1 (MQ=255)
                                                                                             tGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGt        >  1:4085441/1‑100 (MQ=255)
                                                                                             tGCTGAACCCGCAGCTACTGGGGCGCGTCTGGTTGGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGt        <  1:2122465/100‑1 (MQ=255)
                                                                                              gCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGtg       >  2:3700693/1‑100 (MQ=255)
                                                                                               cTTACCCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGtgc      <  1:1775640/100‑1 (MQ=255)
                                                                                                ttAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGtgct     >  1:1532262/1‑100 (MQ=255)
                                                                                                 tAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGtgctg    >  1:1230053/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GCCAGTTAATGCATCTTTAATCGGGAACTTTCATGAAAGCACCAGGCTTGCCCGCCGATCAACAATTTTTCGCCGATCTGTTCAGCGGCCTGGTGCTTAACCCGCAACTACTGGGGCGCGTCTGGTTTGCCAGCCAGCCTGCCTCGTTGCCGGTGGGCAGTTTATGTATTGATTTTCCCCGTCTGGATATCGTGCTGCG  >  NC_000913/2501098‑2501296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: