Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,509,578 C→T 41.9% intergenic (‑152/‑52) glk ← / → yfeO glucokinase/putative ion channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,509,5780CT41.9% 98.9 / 198.0 174intergenic (‑152/‑52)glk/yfeOglucokinase/putative ion channel protein
Reads supporting (aligned to +/- strand):  ref base C (55/46);  new base T (37/36);  total (92/82)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

Only 100 of 178 total aligned reads displayed.

CTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGC  >  NC_000913/2509479‑2509676
                                                                                                   |                                                                                                  
cTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGcc                                                                                                    <  2:3291771/100‑1 (MQ=255)
    gggCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtttt                                                                                                <  1:2815338/100‑1 (MQ=255)
    gggCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtttt                                                                                                >  2:2599324/1‑100 (MQ=255)
     ggCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCtttttt                                                                                               <  1:2219163/100‑1 (MQ=255)
      gCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTg                                                                                              <  1:3225286/100‑1 (MQ=255)
      gCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTg                                                                                              >  2:551173/1‑100 (MQ=255)
       cATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTg                                                                                              <  1:1679426/99‑1 (MQ=255)
       cATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGc                                                                                             <  1:1030585/100‑1 (MQ=255)
         tAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCaa                                                                                           >  1:2383898/1‑100 (MQ=255)
         tAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCaa                                                                                           >  2:3662306/1‑100 (MQ=255)
         tAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCaa                                                                                           >  2:1075012/1‑100 (MQ=255)
          aacaacaacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAg                                                                                          <  2:1853725/100‑1 (MQ=255)
            caacaacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCt                                                                                        >  2:2851752/1‑100 (MQ=255)
            caacaacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCt                                                                                        >  1:1082441/1‑100 (MQ=255)
            caacaacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATGGCAGCGATGCCTTTTTGCAAGCt                                                                                        <  1:834510/100‑1 (MQ=255)
               caacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTggg                                                                                     >  2:1334471/1‑100 (MQ=255)
                aacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGc                                                                                    <  1:1406839/100‑1 (MQ=255)
                aacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGATgg                                                                                      >  1:236166/1‑98 (MQ=255)
                aacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCCAGCTGGGc                                                                                    >  1:2007597/1‑100 (MQ=255)
                  cGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCaa                                                                                  <  2:1961314/100‑1 (MQ=255)
                    ccGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAAc                                                                                <  2:2383904/100‑1 (MQ=255)
                    ccGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGT‑‑CACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTa                                                                              <  2:1435954/100‑1 (MQ=255)
                    ccGGAAAGGCAGGCTCCCTGTAAATATCGATCGGGTTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTATGCAAGCTGGGCa                                                                                   <  1:795834/97‑1 (MQ=255)
                     cGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACt                                                                               >  2:208012/1‑100 (MQ=255)
                      ggAAAGGCAGCCTTCCTGTAAATATCGATCTGGGTAACACAATTACTTTAAAGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTa                                                                              <  1:3402461/100‑1 (MQ=255)
                        aaaGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAg                                                                            <  2:3371301/100‑1 (MQ=255)
                        aaaGGCAGGCTACCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAg                                                                            <  1:1291646/100‑1 (MQ=255)
                         aatgcaggcTTCCTGTAAATATCTATCTGGGTCATACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAg                                                                            <  1:444035/96‑1 (MQ=255)
                         aaGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGt                                                                           >  1:3631222/1‑100 (MQ=255)
                           ggcaggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTAt                                                                         >  1:1762676/1‑100 (MQ=255)
                           ggcaggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTAt                                                                         >  2:98077/1‑100 (MQ=255)
                            gcaggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATc                                                                        <  2:2142992/100‑1 (MQ=255)
                             caggcTCCCTGTAAATATCGATCTGGGTCATACAATTACTTTATCGCTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATc                                                                        <  1:2880040/99‑1 (MQ=255)
                             caggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCCATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCt                                                                       >  1:2734370/1‑100 (MQ=255)
                              aggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTg                                                                      >  1:2426674/1‑100 (MQ=255)
                               ggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGa                                                                     >  2:2067420/1‑100 (MQ=255)
                                 cTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATAGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATc                                                                        <  1:1982468/95‑1 (MQ=255)
                                  tCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAccc                                                                  <  2:2516496/100‑1 (MQ=255)
                                   cccTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCTAGCTGGGCAAACTAAGTATCTGAcccc                                                                 >  1:526863/1‑100 (MQ=255)
                                     cTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGc                                                               <  1:3846744/100‑1 (MQ=255)
                                      tGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCa                                                              <  2:3250904/100‑1 (MQ=255)
                                       gTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCAt                                                             <  2:3726026/100‑1 (MQ=255)
                                       gTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCAt                                                             <  2:4152556/100‑1 (MQ=255)
                                          aaTATCGATCTCGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCgagatcg                                                          >  1:3278095/1‑94 (MQ=255)
                                           atatCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCTCATAAgg                                                         <  2:2237557/100‑1 (MQ=255)
                                           atatCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAgg                                                         >  1:639021/1‑100 (MQ=255)
                                             atCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTTTTTTTTTTTCTAACTAAGTATCTGACCCCGCATAATGa                                                        >  1:1005241/1‑99 (MQ=255)
                                              tCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAAt                                                      <  2:2351719/100‑1 (MQ=255)
                                              tCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAAt                                                      <  2:3550570/100‑1 (MQ=255)
                                               cGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATa                                                     <  1:1101401/100‑1 (MQ=255)
                                               cGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATa                                                     >  1:1453650/1‑100 (MQ=255)
                                               cGATCTGGGTCACACAATTACTTTATCGTTGCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATATGACGACGCAtctgg                                                         <  1:1846496/96‑5 (MQ=255)
                                                gATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAg                                                    >  2:75482/1‑100 (MQ=255)
                                                gATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAg                                                    >  1:3836882/1‑100 (MQ=255)
                                                gATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAg                                                    >  2:154215/1‑100 (MQ=255)
                                                gATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAg                                                    <  2:4128354/100‑1 (MQ=255)
                                                 aTCTGGGTCACACAATTACTTTATCGTTTTAGCACCAATTGCAGCCATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGa                                                   <  1:2569659/100‑1 (MQ=255)
                                                 aTCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGa                                                   <  2:1905721/100‑1 (MQ=255)
                                                  tCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTAGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGan                                                  <  1:315537/100‑2 (MQ=255)
                                                   cTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAGGGAATAGAAc                                                 <  1:756702/100‑1 (MQ=255)
                                                   cTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAAc                                                 <  1:3719714/100‑1 (MQ=255)
                                                       gTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGc                                             <  2:1446614/100‑1 (MQ=255)
                                                       gTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGc                                             <  1:2181146/100‑1 (MQ=255)
                                                        tCATACAATTATTTTATCTTTTCATCACCAATTGCAGCGATGATTTTTTGAAAGTTGGGCAAATTAAGTATCTGACCCCGCATAAGGAATAGAATATg                                              <  1:3542854/98‑1 (MQ=255)
                                                        tCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCt                                            <  1:3704933/100‑1 (MQ=255)
                                                        tCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCt                                            <  2:3143408/100‑1 (MQ=255)
                                                           cacaATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCa                                         >  2:1131015/1‑100 (MQ=255)
                                                           cacaATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCa                                         >  1:2920220/1‑100 (MQ=255)
                                                           cacaATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCGGACCCCGCATAAGGAATACAACATGCTCCa                                         >  1:778118/1‑100 (MQ=255)
                                                             caATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATc                                       >  2:3935991/1‑100 (MQ=255)
                                                                tttcTTTATTGTTTCAGCATCAATGTCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCcgc                                    <  1:872415/97‑1 (MQ=255)
                                                                 tACTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCcgcg                                   <  2:65591/100‑1 (MQ=255)
                                                                  aCTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGa                                  >  2:3539680/1‑100 (MQ=255)
                                                                   cTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAg                                 <  2:4160014/100‑1 (MQ=255)
                                                                   cTTTATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAg                                 <  1:2374196/100‑1 (MQ=255)
                                                                   cTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAg                                 >  1:3748132/1‑100 (MQ=255)
                                                                    tctATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGc                                <  2:2201597/98‑1 (MQ=255)
                                                                     ttATCGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGcc                               <  2:3061764/100‑1 (MQ=255)
                                                                      tATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCa                              >  1:427707/1‑100 (MQ=255)
                                                                      tATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCa                              <  2:1423162/100‑1 (MQ=255)
                                                                         cGTTTCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGaa                           >  2:4004606/1‑100 (MQ=255)
                                                                         cGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGaa                           >  2:1128778/1‑100 (MQ=255)
                                                                            ttCAGCACCAATTGCAGCGATGCTTTTTTACAAACTGAGCAAACTAAGTATCTGACACCGAATAAGGACTAGCACATGCTCAATctgtggtct                               >  1:2692440/1‑85 (MQ=255)
                                                                            ttCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGGAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCa                        >  1:3810046/1‑100 (MQ=255)
                                                                            ttCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAAcc                         <  2:3423771/99‑1 (MQ=255)
                                                                            ttCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCa                        <  2:3704128/100‑1 (MQ=255)
                                                                            ttCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCa                        >  2:3765295/1‑100 (MQ=255)
                                                                             tCAGCACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAt                       >  2:2565242/1‑100 (MQ=255)
                                                                              cAGCACCAATTGCAGCGATGCCTTTTTTTTTTTTTTTCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAtg                      >  1:4008876/1‑100 (MQ=255)
                                                                                 cACCAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAACCAGAACAAtgttg                   >  1:1642666/1‑100 (MQ=255)
                                                                                 cACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAtgttg                   <  2:639023/100‑1 (MQ=255)
                                                                                  aCCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAtgttgt                  >  2:3599065/1‑100 (MQ=255)
                                                                                  aCCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAtgttgt                  >  2:350322/1‑100 (MQ=255)
                                                                                   ccAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATgttgtt                 >  1:2044436/1‑100 (MQ=255)
                                                                                    cAATTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGtta                >  2:871214/1‑100 (MQ=255)
                                                                                     aaTTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGttat               <  2:169381/100‑1 (MQ=255)
                                                                                      aTTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGttatt              >  1:1253771/1‑100 (MQ=255)
                                                                                      aTTGCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGttat               <  2:2926102/99‑1 (MQ=255)
                                                                                         gCAGCGATGCTTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATc           >  2:338256/1‑100 (MQ=255)
                                                                                                  ccTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCccgc  >  2:4251233/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
CTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGC  >  NC_000913/2509479‑2509676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: