BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,521,967	C→A	100%	A171S (GCG→TCG)	xapR ←	transcriptional activator of xapAB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,521,967	0	C	A	100.0%	464.9 / ‑6.7	138	A171S (GCG→TCG)	xapR	transcriptional activator of xapAB
Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (64/72); total (66/72)

Only 100 of 139 total aligned reads displayed.

GCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA  >  NC_000913/2521871‑2522062

gcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAAcgacga                                                                                              >  1:700018/1‑100 (MQ=255)

 cggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATTAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAt                                                                                             <  1:2324566/100‑1 (MQ=255)

 cggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAt                                                                                             <  2:3119197/100‑1 (MQ=255)

  ggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAtt                                                                                            <  2:3074846/100‑1 (MQ=255)

   gcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGAGGAAAATGATGAGAGATGATGCTCTTCACGCATCGCCACCAGAAACGAAGAtt                                                                                            >  1:3481031/1‑99 (MQ=255)

      ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg                                                                                        <  1:3798730/100‑1 (MQ=255)

      ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg                                                                                        <  1:2267644/100‑1 (MQ=255)

       tGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGc                                                                                       >  2:889733/1‑100 (MQ=255)

        gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa                                                                                      >  2:130135/1‑100 (MQ=255)

        gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa                                                                                      >  1:4190777/1‑100 (MQ=255)

          aaCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAc                                                                                    <  1:2834248/100‑1 (MQ=255)

           aCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATGAATGc                                                                                       >  1:3284646/1‑96 (MQ=255)

           aCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAcc                                                                                   >  2:578417/1‑100 (MQ=255)

            cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                  <  2:375153/100‑1 (MQ=255)

              aaaTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTa                                                                                <  1:1980740/100‑1 (MQ=255)

              aaaTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTa                                                                                >  1:604331/1‑100 (MQ=255)

                 tACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGc                                                                             <  1:2953071/100‑1 (MQ=255)

                    tCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTgg                                                                          <  2:3758327/100‑1 (MQ=255)

                     cGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGt                                                                         <  1:2552003/100‑1 (MQ=255)

                     cGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGt                                                                         >  1:1325092/1‑100 (MQ=255)

                      gTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGCTGATGCTCTTCAGGCATCGCCCACAGCAACGCCGCTTACTGCAACCGTACGCTgg                                                                          >  2:3997096/1‑98 (MQ=255)

                      gTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTa                                                                        <  2:1266562/100‑1 (MQ=255)

                          cGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAc                                                                    >  2:1831837/1‑100 (MQ=255)

                          cGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACAACGATTCATGCAACCGTAAGCTGGTAAAAc                                                                    >  2:1369930/1‑100 (MQ=255)

                           gTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc                                                                   <  2:813051/100‑1 (MQ=255)

                           gTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc                                                                   <  2:2373184/100‑1 (MQ=255)

                            tAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCa                                                                  >  2:3778214/1‑100 (MQ=255)

                             aGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAg                                                                 >  2:4111052/1‑100 (MQ=255)

                                gcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCg                                                              <  2:604333/100‑1 (MQ=255)

                                 cTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCgg                                                             >  2:3274660/1‑100 (MQ=255)

                                 cTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCgg                                                             <  2:351838/100‑1 (MQ=255)

                                  ttCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggt                                                            <  1:1535218/100‑1 (MQ=255)

                                   tCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtg                                                           >  1:1272384/1‑100 (MQ=255)

                                    ccAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtgg                                                          >  2:1101390/1‑100 (MQ=255)

                                     cggCGGGACGGTGGAAAATGATTAGAGATTATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                         <  2:3562586/98‑1 (MQ=255)

                                     cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                         >  2:1427096/1‑100 (MQ=255)

                                     cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                         >  2:2986108/1‑100 (MQ=255)

                                      aGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAAAGACGGTTGATGCATCCGTAAGCTGGTAAAACCAGGCGGTGGtt                                                        <  1:779426/100‑1 (MQ=255)

                                       gCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTc                                                       <  2:2921446/100‑1 (MQ=255)

                                       gCGGGACGGTGGAAAATGATGAGAGATGATGATCTTCTGTCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTc                                                       <  1:2875329/100‑1 (MQ=255)

                                         gggACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTcag                                                     >  1:2585720/1‑98 (MQ=255)

                                          ggACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGt                                                    >  1:105337/1‑100 (MQ=255)

                                            acagTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCg                                                  <  1:3788678/97‑1 (MQ=255)

                                            aCGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCg                                                  <  1:3786986/100‑1 (MQ=255)

                                             cGGTGGAAGATGTTGAGTGATGATACTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGc                                                 >  1:3337500/1‑100 (MQ=255)

                                             cGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGc                                                 >  1:4258173/1‑100 (MQ=255)

                                               gTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCa                                               >  2:1629267/1‑100 (MQ=255)

                                               gTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCa                                               >  2:2224521/1‑100 (MQ=255)

                                               gTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCa                                               >  2:2810036/1‑100 (MQ=255)

                                                tGGAAAATGAT‑‑GAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTc                                            >  1:3335369/1‑100 (MQ=255)

                                                 ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAtt                                             >  1:104237/1‑100 (MQ=255)

                                                  gAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGCTCTGTCGCCATTc                                            >  1:1723426/1‑100 (MQ=255)

                                                     aaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGcc                                         <  2:3529663/100‑1 (MQ=255)

                                                     aaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGcc                                         <  1:3692702/100‑1 (MQ=255)

                                                      atgatgAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCa                                        <  2:1038434/100‑1 (MQ=255)

                                                       tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg                                       >  2:3934849/1‑100 (MQ=255)

                                                         atgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCCACCGTCAGCTGGTAAAACCAGTCGGTGGTTCTGCCGCCATTCGCCAGAt                                     >  1:4071979/1‑100 (MQ=255)

                                                         atgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGAt                                     >  1:38978/1‑100 (MQ=255)

                                                            agagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGTCATTCGCCAGATcc                                   >  1:3264857/1‑99 (MQ=255)

                                                             aagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTTGCCAGATCCCg                                 >  1:1026553/2‑100 (MQ=255)

                                                              agaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCgg                                <  2:3267783/100‑1 (MQ=255)

                                                                atgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCa                              <  2:3982297/100‑1 (MQ=255)

                                                                atgatgCTCCTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCGGTCGCCATTCGCCAGATCCCGGCa                              <  1:1324091/100‑1 (MQ=255)

                                                                 tgatgCTCTTCAGGCATCGCCACCAGCAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCgcc                               >  1:3510732/1‑96 (MQ=255)

                                                                   atgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCa                           <  1:306750/100‑1 (MQ=255)

                                                                    tgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCaa                          >  1:2975334/1‑100 (MQ=255)

                                                                    tgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCaa                          <  1:3703723/100‑1 (MQ=255)

                                                                     gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg                         <  2:3616656/100‑1 (MQ=255)

                                                                     gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg                         <  2:899728/100‑1 (MQ=255)

                                                                      ttccgatctcATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAATCCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGt                        <  2:925836/91‑1 (MQ=255)

                                                                      ctctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg                         >  1:1816531/1‑99 (MQ=255)

                                                                      ctctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGt                        >  2:339350/1‑100 (MQ=255)

                                                                         ttCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcg                     <  1:1568972/100‑1 (MQ=255)

                                                                          tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgc                    <  2:2851751/100‑1 (MQ=255)

                                                                          tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgc                    >  2:1275532/1‑100 (MQ=255)

                                                                           cAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg                   >  2:1635776/1‑100 (MQ=255)

                                                                            aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  >  1:1764824/1‑100 (MQ=255)

                                                                            aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  <  2:33996/100‑1 (MQ=255)

                                                                            aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  >  2:2432922/1‑100 (MQ=255)

                                                                            aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  <  1:2761122/100‑1 (MQ=255)

                                                                               cATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGtagatcggaa               >  1:925833/1‑91 (MQ=255)

                                                                                aTCGCCACCAGAAATGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGtt              <  1:2906551/100‑1 (MQ=255)

                                                                                 tCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTc             <  1:4005205/100‑1 (MQ=255)

                                                                                 tCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTc             <  1:2869443/100‑1 (MQ=255)

                                                                                  cGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTcc            <  1:629703/100‑1 (MQ=255)

                                                                                   gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa           >  2:423503/1‑100 (MQ=255)

                                                                                   gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa           >  1:909513/1‑100 (MQ=255)

                                                                                   gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa           >  2:2048663/1‑100 (MQ=255)

                                                                                   gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa           <  2:1537116/100‑1 (MQ=255)

                                                                                    ccaccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAg          <  2:2186596/100‑1 (MQ=255)

                                                                                    ccaccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAg          >  2:1563865/1‑100 (MQ=255)

                                                                                      accaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCa        >  1:2339423/1‑100 (MQ=255)

                                                                                        caGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAg      >  2:93994/1‑100 (MQ=255)

                                                                                        caGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAg      <  2:4175344/100‑1 (MQ=255)

                                                                                         aGAAACGACGCTTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGCTCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg     <  1:2817282/100‑1 (MQ=255)

                                                                                         aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg     <  1:2036923/100‑1 (MQ=255)

                                                                                         aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg     <  2:3103007/100‑1 (MQ=255)

                                                                                          gAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGc    <  2:3454849/100‑1 (MQ=255)

                                                                                            aaCGACGATTGATGCAACCGTAAGCTGCTAAAACCAGTCAGTGGTTATGTCGCCATTTGCCAGATCCCAGCATCACGTTCGTGGCGCTCCAGAAAgg     >  1:3468430/1‑97 (MQ=255)

                                                                                            aaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCa  >  2:3033087/1‑100 (MQ=255)

GCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA  >  NC_000913/2521871‑2522062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`