Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,597,891 A→T 38.4% T325T (ACT→ACA bamC ← BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,597,8910AT38.4% 118.8 / 151.1 142T325T (ACT→ACAbamCBamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (41/46);  new base T (27/27);  total (69/73)
Fisher's exact test for biased strand distribution p-value = 8.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 151 total aligned reads displayed.

TATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCG  >  NC_000913/2597792‑2597990
                                                                                                   |                                                                                                   
tatcaGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCaga                                                                                                     >  2:1096085/1‑100 (MQ=255)
  tcaGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCAGAGTCagaac                                                                                                   <  2:3727001/100‑3 (MQ=255)
  tcaGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCAGAGTCagaac                                                                                                   <  2:633257/100‑3 (MQ=255)
   caGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTa                                                                                                  <  2:3910079/100‑1 (MQ=255)
   caGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTa                                                                                                  <  1:3633329/100‑1 (MQ=255)
    aGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAACCGCAGCCTGGAAGACAGCTACCAGCGCAACGTTCTGACTCCGAGTCAGAGTAt                                                                                                 >  1:4155419/1‑100 (MQ=255)
    aGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTAt                                                                                                 <  2:2602066/100‑1 (MQ=255)
    aGAAAAAAGGGCCGGATGAGTCCAGCCCTGTATTTGTACTTGCTAAACGCAGCCTGGAAGACAGCTACCCGCGCGCCGTTCTGACTCAGAGTCagaacg                                                                                                  <  1:271131/99‑4 (MQ=255)
     gAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATg                                                                                                >  2:1378334/1‑100 (MQ=255)
     gAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATg                                                                                                >  1:3254620/1‑100 (MQ=255)
     gAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATg                                                                                                <  1:3011859/100‑1 (MQ=255)
        aaaaGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCATCGCGTCGTTCTGACTCTGAGGCAGTGTATGAcc                                                                                             >  1:3829706/1‑100 (MQ=255)
        aaaaGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGAcc                                                                                             <  1:1006715/100‑1 (MQ=255)
         aaaGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCt                                                                                            <  1:2342179/100‑1 (MQ=255)
           aGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGGAGCCTGGAAGACAGCTACCATCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCttg                                                                                          <  1:3009034/100‑2 (MQ=255)
            gggCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGGAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTAGCTCTGAGTCAGAGTATGACCt                                                                                            >  1:635708/1‑97 (MQ=255)
             ggCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCg                                                                                        >  2:2227451/1‑100 (MQ=255)
             ggCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCg                                                                                        <  1:1353094/100‑1 (MQ=255)
             ggCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCg                                                                                        >  2:1086347/1‑100 (MQ=255)
              gctggaatcatCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAATACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCgg                                                                                       >  1:740417/11‑100 (MQ=255)
              gCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCgg                                                                                       >  1:3063672/1‑100 (MQ=255)
              gCCGGATGATTCCAGCACTGTATTTTTGCTTGCTAAACGCAGCTTGGTAGACAGCTACTATCGCGTCGTTCTGATTCTGAGTCGGGGTATGACCTTTc                                                                                         >  1:1032394/1‑98 (MQ=255)
               ccGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGGAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGa                                                                                      <  1:3261085/100‑1 (MQ=255)
                cGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGAt                                                                                     >  2:486548/1‑100 (MQ=255)
                 ggATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCATCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGacc                                                                                    <  2:2892416/100‑3 (MQ=255)
                 ggATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATc                                                                                    <  2:2766128/100‑1 (MQ=255)
                 ggATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATc                                                                                    >  1:4157993/1‑100 (MQ=255)
                 ggATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATc                                                                                    >  2:232222/1‑100 (MQ=255)
                     gatTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATg                                                                                <  2:3190109/100‑1 (MQ=255)
                      atTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATg                                                                                >  1:980105/1‑99 (MQ=255)
                      atTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGa                                                                               >  2:2954897/1‑100 (MQ=255)
                         ccAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACt                                                                            <  1:1184581/100‑1 (MQ=255)
                         ccAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACt                                                                            >  1:2270844/1‑100 (MQ=255)
                         ccAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACt                                                                            >  1:3221318/1‑100 (MQ=255)
                            gCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTa                                                                         >  2:4262331/1‑100 (MQ=255)
                            gCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTa                                                                         >  1:2308712/1‑100 (MQ=255)
                             cccTTTATTTTTACTTGCTAAACGCATCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTa                                                                         <  1:1514374/99‑1 (MQ=255)
                             cccTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAg                                                                        <  2:1606499/100‑1 (MQ=255)
                               cTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGGCTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGgc                                                                      <  2:883990/100‑1 (MQ=255)
                               cTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGgc                                                                      <  1:695086/100‑1 (MQ=255)
                               cTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGgc                                                                      <  2:2270850/100‑1 (MQ=255)
                                tGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGgct                                                                     >  2:358229/1‑100 (MQ=255)
                                    tttttACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGctgatg                                                                 >  2:3563950/1‑100 (MQ=255)
                                     ttttACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCtgctgc                                                                >  1:36923/1‑100 (MQ=255)
                                       ttACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCg                                                               <  1:14405/99‑1 (MQ=255)
                                        tACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGt                                                             <  1:4090087/100‑1 (MQ=255)
                                              cTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTa                                                       >  1:168279/1‑100 (MQ=255)
                                                aaaCGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTaaa                                                     <  2:1750212/100‑1 (MQ=255)
                                                aaaCGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTaaa                                                     >  2:1208392/1‑100 (MQ=255)
                                                   cGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCg                                                  >  2:2754134/1‑100 (MQ=255)
                                                     cAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGcc                                                >  1:1343078/1‑100 (MQ=255)
                                                      aGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCa                                               <  1:4234285/100‑1 (MQ=255)
                                                         cTGGAGGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAAcc                                            >  1:1963915/1‑100 (MQ=255)
                                                         cTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAAcc                                            >  1:431062/1‑100 (MQ=255)
                                                          tGGAAGACAGCTTCTAGCGCGTCGTTCTGACGCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCaa                                              <  1:2314729/97‑1 (MQ=255)
                                                           ggAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg                                          <  1:1500762/100‑1 (MQ=255)
                                                           ggAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg                                          <  1:429587/100‑1 (MQ=255)
                                                            gAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGc                                         <  2:3221330/100‑1 (MQ=255)
                                                             aaGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCa                                        >  1:1387607/1‑100 (MQ=255)
                                                              aGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAg                                       <  2:33345/100‑1 (MQ=255)
                                                               gACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGt                                      <  2:1635740/100‑1 (MQ=255)
                                                                  aGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTta                                   <  2:4226760/100‑1 (MQ=255)
                                                                  aGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTta                                   <  2:2722495/100‑1 (MQ=255)
                                                                   gCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTtat                                  <  1:1939972/100‑1 (MQ=255)
                                                                   gCTACCAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTtat                                  <  1:4100591/100‑1 (MQ=255)
                                                                   gCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTtat                                  >  1:1002431/1‑100 (MQ=255)
                                                                   gCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTtat                                  <  2:3036412/100‑1 (MQ=255)
                                                                   gCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTtat                                  >  1:319374/1‑100 (MQ=255)
                                                                    cTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTtata                                 <  1:3150183/100‑1 (MQ=255)
                                                                    cTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTtata                                 <  2:2473589/100‑1 (MQ=255)
                                                                     tACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAg                                <  1:2530857/100‑1 (MQ=255)
                                                                     tACCAGCGCGTCGTTCTGACACTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAg                                >  1:2605616/1‑100 (MQ=255)
                                                                       ccAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTc                              >  1:2446417/1‑100 (MQ=255)
                                                                       ccAGCGCGTCGTTCTGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTc                              >  2:705632/1‑100 (MQ=255)
                                                                       ccAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCCGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTc                              >  1:2334474/1‑100 (MQ=255)
                                                                            gtcatactCTGACTCAGAGTCAGAGTATGACCTTTCGCATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCgg                         >  1:2276332/8‑100 (MQ=255)
                                                                            gcgTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCgg                         <  2:926644/100‑1 (MQ=255)
                                                                             cgtcgtTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGa                        <  2:1762985/100‑1 (MQ=255)
                                                                                 gtTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGcc                    <  2:502587/100‑1 (MQ=255)
                                                                                  cacTGACTCAGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCa                   >  1:2141518/3‑100 (MQ=255)
                                                                                   tCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACGGGATGCCAg                  >  1:4213467/1‑100 (MQ=255)
                                                                                     tGACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGc                <  2:1852252/100‑1 (MQ=255)
                                                                                     tGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGc                <  2:2517896/100‑1 (MQ=255)
                                                                                     tGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGc                <  1:1290984/100‑1 (MQ=255)
                                                                                      gtctctGAGTCAGTGTATGACCTTTCGTATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGTCAACCTGCAGTTTATAGTCACCGGATGCCAGGcc               <  2:3005075/98‑1 (MQ=255)
                                                                                      gACTCTGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGcc               >  1:1137726/1‑100 (MQ=255)
                                                                                      gACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGcc               <  1:1734657/100‑1 (MQ=255)
                                                                                      gACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGcc               >  2:2081438/1‑100 (MQ=255)
                                                                                      agctctGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGcc               <  2:241135/98‑1 (MQ=255)
                                                                                        ctctGAGTCAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTg             >  2:4026622/1‑100 (MQ=255)
                                                                                          ctGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGa           >  1:93161/1‑100 (MQ=255)
                                                                                           tGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGAt          <  1:2081434/100‑1 (MQ=255)
                                                                                            gAGTCAGTGTATGATCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGAt          <  2:4214086/99‑1 (MQ=255)
                                                                                             aGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGAtcg        <  2:1851402/100‑1 (MQ=255)
                                                                                               tCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGAtcgct      <  2:1985319/100‑1 (MQ=255)
                                                                                                cAGTGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGAtcgctc     <  1:592390/100‑1 (MQ=255)
                                                                                                cAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGAtcgctc     >  1:1979066/1‑100 (MQ=255)
                                                                                                 agagTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGAtcgctcg    <  1:3784967/100‑1 (MQ=255)
                                                                                                 agagTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGAtcgctcg    <  1:1926457/100‑1 (MQ=255)
                                                                                                   agTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTcgcg  <  1:748751/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCG  >  NC_000913/2597792‑2597990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: