Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,810,758 G→A 100% intergenic (+79/‑12) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,7580GA98.8% 511.2 / ‑4.3 155intergenic (+79/‑12)ygaH/mprAputative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (80/70);  total (83/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAT  >  NC_000913/2810662‑2810849
                                                                                                |                                                                                           
gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTc                                                                                          <  2:3545399/100‑1 (MQ=255)
   aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATa                                                                                       >  2:3512531/1‑100 (MQ=255)
   aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATa                                                                                       <  2:3444400/100‑1 (MQ=255)
    tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATAc                                                                                      >  1:3772259/1‑100 (MQ=255)
     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATAcc                                                                                     >  1:1246988/1‑100 (MQ=255)
     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATAc                                                                                      >  1:3629254/1‑99 (MQ=255)
      gCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATAccc                                                                                    >  1:2826710/1‑100 (MQ=255)
       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCa                                                                                   >  1:2522580/1‑100 (MQ=255)
       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCa                                                                                   >  2:3725718/1‑100 (MQ=255)
       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCa                                                                                   >  2:3130199/1‑100 (MQ=255)
           tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATg                                                                               <  1:2456943/100‑1 (MQ=255)
            atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATgg                                                                              >  2:2712275/1‑100 (MQ=255)
              ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGAt                                                                            >  2:171654/1‑100 (MQ=255)
              ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGAt                                                                            <  2:380112/100‑1 (MQ=255)
              ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGAt                                                                            <  1:249177/100‑1 (MQ=255)
                aaCCCACAAGAATCATTTTTTTTTTTTTTTTCATTTACTTTATTTGTCACTGTCTTTACTATTTCGTCTGAAATTAATAATGTCATACTCAAATgca                                                                             >  1:524122/1‑95 (MQ=255)
                 aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGt                                                                         >  2:389040/1‑100 (MQ=255)
                  cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGtt                                                                        >  2:1325443/1‑100 (MQ=255)
                  cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGtt                                                                        >  1:11526/1‑100 (MQ=255)
                   ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGt                                                                         <  1:606304/98‑1 (MQ=255)
                   ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTc                                                                       <  2:597926/100‑1 (MQ=255)
                   ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTc                                                                       >  2:2378381/1‑100 (MQ=255)
                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCg                                                                      <  2:4153848/100‑1 (MQ=255)
                     acaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATAACCAAATGGATAGtt                                                                        >  1:55954/1‑97 (MQ=255)
                      caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGtt                                                                    >  1:1058232/1‑100 (MQ=255)
                          aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACg                                                                <  1:3803616/100‑1 (MQ=255)
                            tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGcc                                                              <  1:3920049/100‑1 (MQ=255)
                            tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGcc                                                              <  1:2775651/100‑1 (MQ=255)
                            tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGc                                                               <  1:2454199/99‑1 (MQ=255)
                             cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGccc                                                             >  2:1699595/1‑100 (MQ=255)
                              aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                            >  2:1132579/1‑100 (MQ=255)
                              aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                            <  2:3077312/100‑1 (MQ=255)
                               tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCAt                                                           >  2:2649000/1‑100 (MQ=255)
                               tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCAt                                                           <  2:1127428/100‑1 (MQ=255)
                               tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCAt                                                           >  2:2904907/1‑100 (MQ=255)
                                ttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCAtt                                                          >  2:3161662/1‑100 (MQ=255)
                                 tttGTAAAACAATACATTTACTTTATTTGTCAGTGTCGTTAGTATATCGGCTGACATTATTAAGGTCATACCCATCTGGATAGTTCGTTTACGCCCATTg                                                         <  1:157386/100‑1 (MQ=255)
                                 tttCTAAAACAATACATTTACTTTATTTGTCACTTTCGTTACTATATCGGCTCAAATTAATAAAGTCATACCCAAATGGATAGCTCGCTTACGCCCATTg                                                         <  1:2634829/100‑1 (MQ=255)
                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                      >  1:4074437/1‑100 (MQ=255)
                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                      >  2:2826127/1‑100 (MQ=255)
                                     tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                     <  2:123218/100‑1 (MQ=255)
                                     tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                     <  1:2130173/100‑1 (MQ=255)
                                     tAAAACAATACAATTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                     <  2:3881469/100‑1 (MQ=255)
                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                    >  1:1832817/1‑100 (MQ=255)
                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                    >  1:3538083/1‑100 (MQ=255)
                                       aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                   >  2:238981/1‑100 (MQ=255)
                                            aTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTa                                              >  2:1436769/1‑100 (MQ=255)
                                             tACATTTACTTTATTTGTCACTGTCGTTACTATATAAGTTGAAATTAATAAGGTCATACCAAAATGGATAGTTCGTTTACGCCCCTTGAACAAATCCTaa                                             >  1:3919327/1‑100 (MQ=255)
                                               cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                           <  1:1928759/100‑1 (MQ=255)
                                                aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                          <  1:132085/100‑1 (MQ=255)
                                                 tttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAtt                                         >  1:1378032/1‑100 (MQ=255)
                                                   tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                       <  2:3316208/100‑1 (MQ=255)
                                                   tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                       <  1:733222/100‑1 (MQ=255)
                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                      <  1:3942416/100‑1 (MQ=255)
                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                      >  1:236779/1‑100 (MQ=255)
                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                      >  1:1765344/1‑100 (MQ=255)
                                                     cTTTTTTTGTCACTGTTGTTACTATATCGGTTGAAATTAATAAAGTCATACGCAAATGGATAGTTCGTTTACGCCCATTGATCAAATGCTAAAATTTcgc                                     <  1:3389453/100‑1 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                     >  1:4055165/1‑100 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                     <  2:1898664/100‑1 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                     >  2:152840/1‑100 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                     >  2:1177205/1‑100 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATAACCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                      >  1:2420864/1‑99 (MQ=255)
                                                     cTTTATTTGTCACTGTCGTTACTATAGCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                     >  2:2667001/1‑100 (MQ=255)
                                                      tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                    >  1:3960338/1‑100 (MQ=255)
                                                       ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                     <  1:3673439/98‑1 (MQ=255)
                                                       ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                   <  2:2597685/100‑1 (MQ=255)
                                                        tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                  >  2:3504898/1‑100 (MQ=255)
                                                        tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                  <  2:1956421/100‑1 (MQ=255)
                                                         aTTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCa                                 >  2:1652485/1‑100 (MQ=255)
                                                            tGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgcc                              >  2:1450600/1‑100 (MQ=255)
                                                               cACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                           >  1:2533924/1‑100 (MQ=255)
                                                                aCTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                          >  1:899614/1‑100 (MQ=255)
                                                                   gTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                       >  1:3461438/1‑100 (MQ=255)
                                                                    tCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGaa                      <  1:1784444/100‑1 (MQ=255)
                                                                      gTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                    <  1:3806451/100‑1 (MQ=255)
                                                                        tACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtt                  <  1:3409474/100‑1 (MQ=255)
                                                                        tACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtt                  >  1:1486143/1‑100 (MQ=255)
                                                                        tACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtt                  >  2:4236936/1‑100 (MQ=255)
                                                                         aCTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                 <  1:3758678/100‑1 (MQ=255)
                                                                          cTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACACATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                <  2:3008749/100‑1 (MQ=255)
                                                                            atatCTGCTGAAATTAATAAAGTCCTACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc              <  1:1288317/100‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc              <  1:3024221/100‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc              <  2:4004967/100‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc              <  2:2568350/100‑1 (MQ=255)
                                                                            atatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc              <  1:130444/100‑1 (MQ=255)
                                                                             tatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc              <  2:2542554/99‑1 (MQ=255)
                                                                             tatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt             <  1:2169807/100‑1 (MQ=255)
                                                                             tatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt             <  1:1363667/100‑1 (MQ=255)
                                                                              atCGGCTGAAATTAATAAAGTCATACCCGAATGGATAGTTCGTTTACGCCCATTGAACATATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc              <  1:214576/98‑1 (MQ=255)
                                                                              atCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt            <  2:1089931/100‑1 (MQ=255)
                                                                              atCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt            >  2:1325963/1‑100 (MQ=255)
                                                                               tCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTa           <  2:2496090/100‑1 (MQ=255)
                                                                                 ggCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc         <  1:3267373/100‑1 (MQ=255)
                                                                                 ggCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAGACTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc         >  1:3313755/1‑100 (MQ=255)
                                                                                  gCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa        <  1:3490304/100‑1 (MQ=255)
                                                                                    tGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg      >  1:2963741/1‑100 (MQ=255)
                                                                                    tGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg      >  1:1490526/1‑100 (MQ=255)
                                                                                      aaaTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag    <  2:4201131/100‑1 (MQ=255)
                                                                                       aaTTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga   <  1:2734085/100‑1 (MQ=255)
                                                                                        aTTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt  >  2:2096916/1‑100 (MQ=255)
                                                                                                |                                                                                           
GTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAT  >  NC_000913/2810662‑2810849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: