Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,810,872 C→T 15.9% H35Y (CAC→TAC) ‡ mprA → transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,8720CT15.9% 431.2 / 59.0 174H35Y (CAC→TAC) mprAtranscriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base C (74/72);  new base T (12/16);  total (86/88)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01

Only 100 of 204 total aligned reads displayed.

GATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGG  >  NC_000913/2810773‑2810971
                                                                                                   |                                                                                                   
gATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgc                                                                                                     <  2:1786961/100‑1 (MQ=255)
 aTAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgca                                                                                                    <  2:2692814/100‑1 (MQ=255)
     ttCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatg                                                                                                <  2:1832821/100‑1 (MQ=255)
     ttCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatg                                                                                                <  2:372858/100‑1 (MQ=255)
      tCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatgc                                                                                               >  2:1388674/1‑100 (MQ=255)
       cGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatgca                                                                                              <  2:2522587/100‑1 (MQ=255)
        gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatcacc                                                                                             >  2:4119713/1‑96 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                          <  1:3121075/100‑2 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                          <  1:1403575/100‑2 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagc                                                                                          >  1:1230338/1‑100 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAAGTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagc                                                                                          <  1:1830195/100‑1 (MQ=255)
           tACGCACATTGAACAAATGCTAAACTTGCGCGCCAGCCGCCACGAAGATTTTCTTTATCAGGAGAGCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                          <  1:905236/100‑2 (MQ=255)
            aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                         <  2:446646/100‑3 (MQ=255)
            aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                         <  2:3290536/100‑3 (MQ=255)
            aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                         <  1:2526689/100‑3 (MQ=255)
            aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                         <  1:12024/100‑3 (MQ=255)
            aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCAACGAAGATTTTCCTTATCAGGAGATCCTTCTGAATCGTCTTTGCATGTGCATGCAaagac                                                                                         <  2:235275/100‑3 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacg                                                                                        <  1:1334562/100‑4 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaag                                                                                           <  1:3574313/97‑1 (MQ=255)
              gCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacga                                                                                       <  2:1598178/100‑5 (MQ=255)
              gCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacga                                                                                       <  1:3161650/100‑5 (MQ=255)
              gCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaag                                                                                       >  1:2343958/1‑100 (MQ=255)
               cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgag                                                                                      <  1:238980/100‑6 (MQ=255)
               cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaagc                                                                                      >  2:752814/1‑100 (MQ=255)
                  aTTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAgctgc                                                                                   >  2:2052465/1‑100 (MQ=255)
                  aTTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAgctgc                                                                                   >  1:3651682/1‑100 (MQ=255)
                  aTTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAgctgc                                                                                   >  2:3251466/1‑100 (MQ=255)
                    tGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGctgctg                                                                                 >  2:1706133/1‑100 (MQ=255)
                       aCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgag                                                                              <  2:2627078/100‑1 (MQ=255)
                        cAAATGCTAAAATTTCGCGCCAGCCGCCACTAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgag                                                                              <  1:2666991/99‑1 (MQ=255)
                        cAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgaga                                                                             >  1:393606/1‑100 (MQ=255)
                          aaTGCTAAAATTTCGCGCCAGCCGCCACGTAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAAc                                                                           <  1:2566257/100‑1 (MQ=255)
                          aaTGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAAc                                                                           <  1:2835969/100‑1 (MQ=255)
                             gCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGc                                                                        >  1:3013330/1‑100 (MQ=255)
                             gCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGc                                                                        >  1:1644624/1‑100 (MQ=255)
                             gCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGc                                                                        >  2:3081273/1‑100 (MQ=255)
                              cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCa                                                                       >  2:2761890/1‑100 (MQ=255)
                               tAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTTTCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCaa                                                                      <  2:2997383/100‑1 (MQ=255)
                                       cgcgCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGc                                                              <  2:3629270/100‑1 (MQ=255)
                                       cgcgCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGc                                                              <  2:1347345/100‑1 (MQ=255)
                                       cgcgCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGc                                                              <  1:690148/100‑1 (MQ=255)
                                       cgcgCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGc                                                              >  1:2186676/1‑100 (MQ=255)
                                          gcCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGa                                                           <  2:2444269/100‑1 (MQ=255)
                                           ccAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGaa                                                          <  2:1477013/100‑1 (MQ=255)
                                            cAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAg                                                         >  1:291534/1‑100 (MQ=255)
                                             aGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAgg                                                        >  2:2829487/1‑100 (MQ=255)
                                              gccgccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGc                                                       <  1:2093269/100‑1 (MQ=255)
                                               ccgccACGAATATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCt                                                      <  1:4172903/100‑1 (MQ=255)
                                                gatctcGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTc                                                     <  1:3049977/95‑1 (MQ=255)
                                                  ccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAg                                                   <  1:1915420/100‑1 (MQ=255)
                                                       aaGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAt                                              >  1:3086402/1‑100 (MQ=255)
                                                         gATTTTCTTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTa                                            >  2:2242802/1‑100 (MQ=255)
                                                         gATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTa                                            <  2:2968808/100‑1 (MQ=255)
                                                           ttttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTatc                                          <  2:2892332/100‑3 (MQ=255)
                                                           ttttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc                                          <  2:827438/100‑1 (MQ=255)
                                                           ttttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc                                          >  1:2659046/1‑100 (MQ=255)
                                                           tgttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc                                          <  2:2294994/98‑1 (MQ=255)
                                                            tttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACg                                         <  1:2531875/100‑1 (MQ=255)
                                                            tttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACg                                         >  2:3214220/1‑100 (MQ=255)
                                                              tCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgag                                       <  2:1490530/100‑1 (MQ=255)
                                                               ccTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgaga                                      >  1:107894/1‑100 (MQ=255)
                                                               ccTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgaga                                      <  1:1624038/100‑1 (MQ=255)
                                                               ccTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgaga                                      <  2:2513147/100‑1 (MQ=255)
                                                                  tATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgt                                   >  1:1789773/1‑100 (MQ=255)
                                                                     cAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt                                <  1:2691333/100‑1 (MQ=255)
                                                                      aGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt                               <  2:3886740/100‑1 (MQ=255)
                                                                      aGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt                               >  2:3185254/1‑100 (MQ=255)
                                                                      aGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt                               <  1:1177202/100‑1 (MQ=255)
                                                                       ggAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt                              <  2:3910309/100‑1 (MQ=255)
                                                                       ggAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt                              >  1:4038508/1‑100 (MQ=255)
                                                                       ggAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt                              >  1:2786053/1‑100 (MQ=255)
                                                                          gaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATg                           <  1:3884979/100‑1 (MQ=255)
                                                                           aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                          <  1:1734440/100‑1 (MQ=255)
                                                                           aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                          <  1:438385/100‑1 (MQ=255)
                                                                            tCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGc                         <  1:3512518/100‑1 (MQ=255)
                                                                            tCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGc                         >  1:711038/1‑100 (MQ=255)
                                                                             ccTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCCATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCg                        >  2:1556793/1‑100 (MQ=255)
                                                                                  tGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt                   <  1:1428558/100‑1 (MQ=255)
                                                                                    agcttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGTGATTAACGAGACGTTGTTTATGGCGTTg                     >  1:1028440/7‑96 (MQ=38)
                                                                                    actaaacTTTACATACACATGCAAATCAAGCTGTTAAAAAAAAAAAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt                   <  1:3491922/92‑1 (MQ=255)
                                                                                     cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc                >  2:2966219/1‑100 (MQ=255)
                                                                                     cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc                >  2:3508982/1‑100 (MQ=255)
                                                                                     cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc                >  2:3663768/1‑100 (MQ=255)
                                                                                      cttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg               >  2:3839867/5‑100 (MQ=255)
                                                                                       ttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGc              >  2:2502376/4‑100 (MQ=255)
                                                                                       cGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGc              <  2:4074459/100‑1 (MQ=255)
                                                                                       cGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGc              >  2:325895/1‑100 (MQ=255)
                                                                                        gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCt             >  1:4004919/1‑100 (MQ=255)
                                                                                        gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCt             >  2:2214322/1‑100 (MQ=255)
                                                                                         gcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg            >  2:4014060/2‑100 (MQ=255)
                                                                                             tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGGTTAACGAGACGTTGTTTATGGAGTTGATTACGCTGGa          >  1:3631100/1‑98 (MQ=255)
                                                                                             tgcatgcaCATGCAAAGCAAGCTGCTAGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg         >  2:2598817/1‑99 (MQ=255)
                                                                                              gcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtc       >  2:1087936/1‑100 (MQ=255)
                                                                                               catgcacatgcaAAGTAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct      >  1:2290564/1‑100 (MQ=255)
                                                                                               catgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct      >  2:530373/1‑100 (MQ=255)
                                                                                               catgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct      >  1:2945428/1‑100 (MQ=255)
                                                                                                 tgcacatccaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGTGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtc       <  1:3318099/97‑1 (MQ=255)
                                                                                                  gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg   <  1:3235898/100‑1 (MQ=255)
                                                                                                  gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg   <  1:1132576/100‑1 (MQ=255)
                                                                                                   cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg  <  2:2451224/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGG  >  NC_000913/2810773‑2810971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: