Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,827,362 A→G 46.4% M1M (ATG→GTG) † srlB → glucitol/sorbitol‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,827,3620AG46.4% 27.3 / 242.7 183M1M (ATG→GTG) †srlBglucitol/sorbitol‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base A (58/40);  new base G (46/39);  total (104/79)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 184 total aligned reads displayed.

TGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCT  >  NC_000913/2827264‑2827460
                                                                                                  |                                                                                                  
   cccTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGAc                                                                                                >  2:3437556/1‑100 (MQ=255)
   cccTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGAc                                                                                                <  2:192035/100‑1 (MQ=255)
   cccTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGAc                                                                                                >  2:932183/1‑100 (MQ=255)
    gattttGTACTGGTGGGCTGTTTTTTATAGGGCGCGTAACCTGTACTGATCGACTGATTTTTCTCCGGTTTTATCTATCAATAGATGCTCAAACATGAc                                                                                                <  1:1846987/94‑1 (MQ=255)
       tCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGtt                                                                                            <  1:2664201/100‑1 (MQ=255)
       tCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGtt                                                                                            >  1:1683232/1‑100 (MQ=255)
         tGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGttat                                                                                          <  2:113476/100‑1 (MQ=255)
             cTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCCttatttat                                                                                      >  1:1820011/1‑100 (MQ=255)
              tGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATc                                                                                     <  2:2171581/100‑1 (MQ=255)
              tGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATc                                                                                     <  1:969911/100‑1 (MQ=255)
               ggTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGACACGTGACCGTTATTTATc                                                                                     <  1:4056006/99‑1 (MQ=255)
               ggTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCa                                                                                    >  1:1574735/1‑100 (MQ=255)
               ggTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCa                                                                                    <  1:3284578/100‑1 (MQ=255)
                gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAg                                                                                   <  2:523359/100‑1 (MQ=255)
                gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAg                                                                                   >  2:3778128/1‑100 (MQ=255)
                gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAg                                                                                   <  1:511211/100‑1 (MQ=255)
                gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAg                                                                                   <  1:2657853/100‑1 (MQ=255)
                  gAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGac                                                                                 <  1:3880948/100‑1 (MQ=255)
                   aGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGacc                                                                                <  1:3213982/100‑1 (MQ=255)
                    gCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGacca                                                                               >  1:3576629/1‑100 (MQ=255)
                     ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGaccac                                                                              >  2:26803/1‑100 (MQ=255)
                     ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGaccac                                                                              >  2:26944/1‑100 (MQ=255)
                     ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGaccac                                                                              >  2:3945032/1‑100 (MQ=255)
                      cGCTTTTTAACCTTCTCGCCGACTGTACTGACCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGCTGCTGAAACGCGACCGTTATTTATCATaccacc                                                                             >  1:2125934/1‑100 (MQ=255)
                      cGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGaccacc                                                                             >  1:1654486/1‑100 (MQ=255)
                      cGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGaccacc                                                                             <  1:2135685/100‑1 (MQ=255)
                       gCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGAccacca                                                                            <  2:1526235/100‑1 (MQ=255)
                         tttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATc                                                                          >  2:2792747/1‑100 (MQ=255)
                         tttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATc                                                                          >  2:3268016/1‑100 (MQ=255)
                         tttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATc                                                                          <  2:3049493/100‑1 (MQ=255)
                         tttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATc                                                                          <  2:229305/100‑1 (MQ=255)
                          ttttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCa                                                                         <  2:17613/100‑1 (MQ=255)
                           tttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCAc                                                                        >  2:1027964/1‑100 (MQ=255)
                             tAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCAccc                                                                      >  1:3427127/1‑100 (MQ=255)
                             tAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCAccc                                                                      >  2:130999/1‑100 (MQ=255)
                             tAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCAccc                                                                      >  1:1625192/1‑100 (MQ=255)
                              aaCCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCg                                                                     >  2:4072714/1‑100 (MQ=255)
                              aaCCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCg                                                                     <  2:4217050/100‑1 (MQ=255)
                                ccGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTa                                                                   >  1:1808384/1‑100 (MQ=255)
                                ccGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTa                                                                   >  2:2534751/1‑100 (MQ=255)
                                  ggCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATc                                                                 >  1:1361131/1‑100 (MQ=255)
                                   gcgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCg                                                                >  2:1966044/1‑100 (MQ=255)
                                    cgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCgg                                                               >  1:2792900/1‑100 (MQ=255)
                                      cgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGgcg                                                             <  2:1415703/100‑1 (MQ=255)
                                         cGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGa                                                          >  2:3696248/1‑100 (MQ=255)
                                         cGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGa                                                          >  1:3243207/1‑100 (MQ=255)
                                          gACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGGCCACCATCACCCGTATCGGCGCGAg                                                         <  2:2495867/100‑1 (MQ=255)
                                            cTGTACTGATCGCCTGGTTTGCCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACAGGTATTTATCAGACCACCATCACCCGTATCGGcgcg                                                           >  1:2447975/1‑96 (MQ=255)
                                              gTACTGATCGCCTGGTTTGTCTCCTGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGcc                                                     <  1:308563/100‑1 (MQ=255)
                                              gTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGcc                                                     <  2:3788911/100‑1 (MQ=255)
                                              gTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGcc                                                     >  2:654182/1‑100 (MQ=255)
                                                 cTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCAtt                                                  >  2:3615934/1‑100 (MQ=255)
                                                  tGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCAtt                                                  <  1:1086437/99‑1 (MQ=255)
                                                   gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCCGACCACCATCACCCGTATCGGCGCGAGTGCCATTGa                                                >  1:2651314/1‑100 (MQ=255)
                                                   gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGa                                                >  1:765416/1‑100 (MQ=255)
                                                   gATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGa                                                >  1:1671686/1‑100 (MQ=255)
                                                      cGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                             >  1:880989/1‑100 (MQ=255)
                                                       gCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGcc                                            <  1:1158349/100‑1 (MQ=255)
                                                        ccTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATTACCGGTATTTATCAGACCACCATCAACCGTATCGGCGCGAGTGCCATTTACGccc                                           >  2:2320990/1‑100 (MQ=255)
                                                         cTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTTACGCCCt                                          >  2:1923947/1‑100 (MQ=255)
                                                          tGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTc                                         <  2:3485954/100‑1 (MQ=255)
                                                            gTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAg                                       <  1:3499402/100‑1 (MQ=255)
                                                            gTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAg                                       <  2:3389272/100‑1 (MQ=255)
                                                             tttGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGt                                      <  1:1545128/100‑1 (MQ=255)
                                                               tGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGa                                    >  2:483525/1‑100 (MQ=255)
                                                               tGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTg                                     >  2:2574005/1‑99 (MQ=255)
                                                                gTCTCCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGAc                                   <  2:1185432/100‑1 (MQ=255)
                                                                gTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGAc                                   <  2:2368707/100‑1 (MQ=255)
                                                                gTCTCCGGTTTTATCTATCAAAAGAGGCTGAAACATTACCGTTATTGATCAGACCACCATCACCCGAATCGGCGCGAGTGCCATTGACGCCCTCAGTGAc                                   <  1:421297/100‑1 (MQ=255)
                                                                 tctcCGGTTTTATCTATCAATAGAGGCTGAAGCGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGAcc                                  <  1:1221081/100‑1 (MQ=255)
                                                                  ctcCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCa                                 >  2:3791486/1‑100 (MQ=255)
                                                                  ctcCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCa                                 >  1:650104/1‑100 (MQ=255)
                                                                  ctcCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCCGTGACCa                                 >  2:3857868/1‑100 (MQ=255)
                                                                   tcCGGTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAg                                >  2:2768326/1‑100 (MQ=255)
                                                                   tcCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAg                                <  1:2399966/100‑1 (MQ=255)
                                                                    cgggTTTTATCTACCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa                               >  2:2895411/3‑100 (MQ=255)
                                                                    ccGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa                               <  1:1826836/100‑1 (MQ=255)
                                                                     cGGTTTTATCTATCAATAGAGGCTGAGACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa                               <  1:962627/99‑1 (MQ=255)
                                                                       gTTTTATCTATCAATGGGGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGc                            <  1:1428575/100‑1 (MQ=255)
                                                                       gTTTTATCTATCAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGc                            >  2:2084232/1‑100 (MQ=255)
                                                                       gTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGc                            <  1:3891423/100‑1 (MQ=255)
                                                                          ttATCTATCAATAGAGGCTGAAACGTCACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtca                         <  1:2381437/100‑1 (MQ=255)
                                                                           tatctatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcat                        >  2:1780555/1‑100 (MQ=255)
                                                                           tatctatcAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcat                        >  1:346875/1‑100 (MQ=255)
                                                                             tctatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcatca                      >  2:219535/1‑100 (MQ=255)
                                                                              ctatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCAc                     <  1:4162761/100‑1 (MQ=255)
                                                                               tatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCAcc                    >  1:2967729/1‑100 (MQ=255)
                                                                               tatcAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCAc                     >  2:731881/1‑99 (MQ=255)
                                                                               tatcAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCAcc                    >  2:3983162/1‑100 (MQ=255)
                                                                                atcAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCt                   <  1:172084/100‑1 (MQ=255)
                                                                                  cAATAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCttt                 >  1:3689830/1‑100 (MQ=255)
                                                                                    aTAGAGGCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCg               <  1:2549722/100‑1 (MQ=255)
                                                                                    aTAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCg               >  1:1783750/1‑100 (MQ=255)
                                                                                      agagGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTg             <  1:1055813/100‑1 (MQ=255)
                                                                                       gagGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGa            <  2:1362476/100‑1 (MQ=255)
                                                                                          gCTGAAACGTGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAgg         <  1:3791469/100‑1 (MQ=255)
                                                                                           cTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgc        <  1:2327952/100‑1 (MQ=255)
                                                                                            tGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcg       >  2:1781712/1‑100 (MQ=255)
                                                                                             gAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcgc      >  2:465630/1‑100 (MQ=255)
                                                                                                 cATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCt  >  1:557925/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
TGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCT  >  NC_000913/2827264‑2827460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: