Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,980,746 C→T 15.6% intergenic (‑111/+18) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,980,7460CT15.6% 443.6 / 56.4 178intergenic (‑111/+18)ygeA/araEAsp/Glu_racemase family protein/arabinose transporter
Reads supporting (aligned to +/- strand):  ref base C (73/76);  new base T (16/12);  total (90/88)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 186 total aligned reads displayed.

GATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGG  >  NC_000913/2980647‑2980844
                                                                                                   |                                                                                                  
gATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGc                                                                                                    <  2:3148042/100‑1 (MQ=255)
gATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGc                                                                                                    <  2:3150397/100‑1 (MQ=255)
   gtgtgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcaca                                                                                                 >  2:3964005/1‑100 (MQ=255)
     gtgtgaaagCAACAAGGCTGAACAAGGAACAACACAAACACCTCAACGGCCAAGTGCCCAAAAAAAAAAAAAAAAAAAAGGGCCGGATGTACAGca                                                                                                   <  1:3103540/88‑1 (MQ=255)
        ttACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCgg                                                                                            <  2:1770458/100‑1 (MQ=255)
        ttACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCgg                                                                                            <  2:2833470/100‑1 (MQ=255)
        ttACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCgg                                                                                            >  2:321440/1‑100 (MQ=255)
        ttACCCCAAAAAGGCTGCATAAGTAACAAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGCAAAAAGGGCCGGATGTACAGCACATc                                                                                               <  1:3795189/97‑1 (MQ=255)
         tACCGCAACAAGGCTTAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAAGCTCTAGTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGc                                                                                           <  2:2553241/100‑1 (MQ=255)
         tACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCACAATCTCTATTAACGAAAAAAGGGACGGATGTACAGCACATCCgg                                                                                            >  1:1335795/1‑99 (MQ=255)
          actgtaacaaGGCTGAATAAGGAACGAGACACACGCCGCAGCGTCCAAGTGCCCATTCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcc                                                                                          <  1:2750739/95‑1 (MQ=25)
          aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGTCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcc                                                                                          <  2:1995670/100‑1 (MQ=38)
          aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcc                                                                                          <  2:2471890/100‑1 (MQ=39)
          aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcc                                                                                          <  2:1919706/100‑1 (MQ=39)
          aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGcc                                                                                          >  1:1515194/1‑100 (MQ=255)
          aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCACGATTAACGAAAAAAGGGCCGGAGGTACAGCACATCCGGc                                                                                           >  1:1142327/1‑99 (MQ=255)
          aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCACAAAAAAAAAAAAAAGGGCCGGATGTACAGCACATCCGGcc                                                                                          <  1:533126/100‑1 (MQ=255)
          aCCGCAACAAGGCTGAATAAAGAACGAGACACACCCCTCAACGGCCAAGTGCCCAATCCCAATTAACGCAAAAAGGGCCGGATGTACAGCACATCCGGc                                                                                           <  1:3193011/99‑1 (MQ=255)
           ccGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGccc                                                                                         >  2:415520/1‑100 (MQ=255)
           ccGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGccc                                                                                         >  1:2186887/1‑100 (MQ=255)
            cGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCg                                                                                        >  2:4016005/1‑100 (MQ=255)
             gCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGt                                                                                       >  2:155402/1‑100 (MQ=255)
              caacaaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcccttt                                                                                      <  2:1976313/100‑4 (MQ=38)
                 caaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGaaa                                                                                   >  1:1301475/1‑100 (MQ=255)
                  aaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTTTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                  <  2:1794163/100‑1 (MQ=255)
                  aaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCCGCCCGTGaaa                                                                                   >  1:3744088/1‑99 (MQ=255)
                  aaGGCGGAATAAGAAACGAGACAAACGCATCAACGGCCAAGTGCCCAATAAAAAAAAAAGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGaaa                                                                                   <  1:3436595/99‑1 (MQ=255)
                   aGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATc                                                                                 >  2:3313904/1‑100 (MQ=255)
                   aGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATc                                                                                 <  1:1856311/100‑1 (MQ=255)
                   aGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCAAATATCTATAAAAGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATc                                                                                 <  1:242608/100‑1 (MQ=255)
                     gCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAg                                                                               <  2:3032189/100‑1 (MQ=255)
                     gCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCAATATAAACAAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAg                                                                               <  1:4141289/100‑1 (MQ=255)
                      cTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGa                                                                              >  1:920466/1‑100 (MQ=255)
                       tGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGAc                                                                             >  2:585514/1‑100 (MQ=255)
                       tGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGAc                                                                             <  2:1103994/100‑1 (MQ=255)
                         aaTAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGAc                                                                             <  1:2030438/98‑1 (MQ=255)
                          aTAAGAAACGAGAAAAACGACGAAACGGCCAAGTGCCAAATCACAATAAAAAAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCa                                                                                <  1:1107365/94‑1 (MQ=255)
                           tAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCg                                                                         >  1:2012637/1‑100 (MQ=255)
                              ggAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGata                                                                      <  2:355765/100‑1 (MQ=255)
                               gAACAAGACAAACGCCTCAACGGCCAAGTGCCCAATAAAAAAAAAAAAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGata                                                                      <  1:3369736/99‑1 (MQ=255)
                                aaCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAtt                                                                    >  2:3768483/1‑100 (MQ=255)
                                aaCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAtt                                                                    >  2:2399201/1‑100 (MQ=255)
                                     gaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCa                                                               >  1:4273715/1‑100 (MQ=255)
                                       cAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAAc                                                             >  1:1318282/1‑100 (MQ=255)
                                        aaaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACt                                                            <  1:2399195/100‑1 (MQ=255)
                                        aaaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCCCAACt                                                            <  1:1415722/100‑1 (MQ=255)
                                          aCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTtc                                                          >  1:1079964/1‑100 (MQ=255)
                                          aCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTtc                                                          >  2:2769030/1‑100 (MQ=255)
                                          aCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTtc                                                          <  1:1094403/100‑1 (MQ=255)
                                              ntCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGc                                                      >  2:3248522/2‑100 (MQ=255)
                                              cTCAACGGCCAAGTGCCCAATAAAAATAAAAAAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCg                                                       <  1:867571/99‑1 (MQ=255)
                                                cAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCt                                                    >  1:3036817/1‑100 (MQ=255)
                                                 aaCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTg                                                   <  2:86638/100‑1 (MQ=255)
                                                   cGGCCAAGTGCCCAATCTCTATTAACGAGAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGcc                                                 <  1:156338/100‑1 (MQ=255)
                                                   cGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGcc                                                 >  1:232736/1‑100 (MQ=255)
                                                    ggCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                                >  1:806095/1‑100 (MQ=255)
                                                    ggCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                                >  1:1316597/1‑100 (MQ=255)
                                                     gCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCAt                                               >  1:2948362/1‑100 (MQ=255)
                                                        aaGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAg                                            <  2:4068214/100‑1 (MQ=255)
                                                         aGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGt                                           >  2:3628518/1‑100 (MQ=255)
                                                            gCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTg                                        <  2:4197836/100‑1 (MQ=255)
                                                             cccAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGc                                       <  1:3628502/100‑1 (MQ=255)
                                                               cAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGt                                     <  2:1200619/100‑1 (MQ=255)
                                                               cAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGt                                     >  1:4198550/1‑100 (MQ=255)
                                                                aaTCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGtt                                    <  2:3938727/100‑1 (MQ=255)
                                                                  tctataaaaaCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCg                                  <  1:530101/92‑1 (MQ=255)
                                                                   ctctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGa                                 <  2:3006028/100‑1 (MQ=255)
                                                                   ctctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGa                                 <  1:1103206/100‑1 (MQ=255)
                                                                    tctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGat                                <  2:1170064/100‑1 (MQ=255)
                                                                    tctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGat                                <  1:2171396/100‑1 (MQ=255)
                                                                     ctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGata                               <  2:620391/100‑1 (MQ=255)
                                                                     ctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGata                               <  2:1399341/100‑1 (MQ=255)
                                                                       aTTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATg                             >  2:1071492/1‑100 (MQ=255)
                                                                        ttAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATAAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGt                            >  2:2837682/1‑100 (MQ=255)
                                                                         tAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGCTATg                             >  1:988436/1‑98 (MQ=255)
                                                                         tAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGtt                           >  2:2060471/1‑100 (MQ=255)
                                                                         tAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGtt                           >  2:4246869/1‑100 (MQ=255)
                                                                          aaCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGt                            <  2:2948374/98‑1 (MQ=255)
                                                                           aCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTcc                         <  2:1778280/100‑1 (MQ=255)
                                                                               aaaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCg                     <  1:1242340/100‑1 (MQ=255)
                                                                                   cgggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGAc                 >  2:1863571/2‑100 (MQ=38)
                                                                                   cgggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGAc                 >  2:208486/2‑100 (MQ=38)
                                                                                    gggCCGGATGTACAGCACATCCGGCCCGTGAAATCTGCCGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCg                     <  1:1434101/95‑1 (MQ=255)
                                                                                     ggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGa                  >  1:2079928/1‑97 (MQ=39)
                                                                                     ggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAt               >  2:1631379/1‑100 (MQ=39)
                                                                                     ggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAt               >  2:1220659/1‑100 (MQ=39)
                                                                                     ggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAt               >  1:1591633/1‑100 (MQ=39)
                                                                                      gCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt              >  2:896401/1‑100 (MQ=255)
                                                                                      gCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTATCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt              >  1:2685218/1‑100 (MQ=255)
                                                                                       ccGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAttt             >  2:540329/1‑100 (MQ=255)
                                                                                         ggATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAttttt           >  1:3778069/1‑100 (MQ=255)
                                                                                           aTGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTgg         <  2:2919979/100‑1 (MQ=255)
                                                                                            tGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGt        <  2:1442649/100‑1 (MQ=255)
                                                                                             gTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGtt       <  2:2649991/100‑1 (MQ=255)
                                                                                              tACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGttt      >  2:3716399/1‑100 (MQ=255)
                                                                                              tACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGttt      >  1:189917/1‑100 (MQ=255)
                                                                                               aCAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTc     >  1:1442778/1‑100 (MQ=255)
                                                                                                cAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTcc    >  1:3944927/1‑100 (MQ=255)
                                                                                                  gCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCgg  >  2:3662150/1‑100 (MQ=255)
                                                                                                  gCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCgg  <  2:3421862/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGG  >  NC_000913/2980647‑2980844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: