Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,981,031 G→T 37.1% C384* (TGC→TGA)  araE ← arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,981,0310GT37.1% 156.6 / 164.0 156C384* (TGC→TGA) araEarabinose transporter
Reads supporting (aligned to +/- strand):  ref base G (51/47);  new base T (32/26);  total (83/73)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 161 total aligned reads displayed.

TGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTA  >  NC_000913/2980932‑2981130
                                                                                                   |                                                                                                   
 gtCAGGAAGGTCGTGCCGATAATCATATTCGACACCCAGTTAGTGGTGTTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCATAGc                                                                                                    >  1:786204/1‑100 (MQ=38)
 gtCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGc                                                                                                    <  2:2931649/100‑1 (MQ=255)
 gtCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCACGGCATTTCAGCGGCTGAATTTCAGAGc                                                                                                    >  1:680414/1‑100 (MQ=255)
 gtCAGGAAGATCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGc                                                                                                    <  2:786206/100‑1 (MQ=255)
  tCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCGGCGGCTGAATTTCAGAGca                                                                                                   >  1:3390987/1‑100 (MQ=255)
   cAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGcac                                                                                                  >  2:2052087/1‑100 (MQ=255)
     ngAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCAGAATTTCAGATCACAg                                                                                                >  1:1821296/2‑100 (MQ=255)
     ggAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAg                                                                                                <  2:3944945/100‑1 (MQ=255)
     ggAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAg                                                                                                >  1:577828/1‑100 (MQ=255)
       aaGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGa                                                                                              <  1:3136823/100‑1 (MQ=255)
            cgcgCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCcac                                                                                         >  2:3320779/1‑100 (MQ=255)
             gcgcCTATAATCATAGTCGACACTCAGTTCCTGGTCGGCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACATGAt                                                                                             <  1:3927203/95‑1 (MQ=25)
              cgcCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTTAATTTCAGATCACAGGATCCacac                                                                                       >  1:2594852/1‑100 (MQ=255)
              cgcCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCacac                                                                                       >  2:3080137/1‑100 (MQ=255)
              cgcCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCacac                                                                                       >  1:1138251/1‑100 (MQ=255)
                 cGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCAcaccac                                                                                    <  2:2854521/100‑1 (MQ=255)
                  gATAATCATAGTCGACGCGCAGGTCGTGGTGGTCGAGCAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTGCAGATCACAGGATCCACACCACt                                                                                   <  1:1548496/100‑1 (MQ=37)
                     aaTCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTggc                                                                                >  2:271882/1‑100 (MQ=255)
                      aTCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTggcg                                                                               >  2:1000582/1‑100 (MQ=255)
                       tCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTggcgc                                                                              >  2:3240289/1‑100 (MQ=255)
                       tCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTggcgc                                                                              >  2:3963634/1‑100 (MQ=255)
                         atatTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTggcgcgg                                                                            <  2:4162459/100‑1 (MQ=255)
                         atatTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTggcgcgg                                                                            <  2:1064794/100‑1 (MQ=255)
                          tatTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTggcgcggc                                                                           <  2:3271673/100‑1 (MQ=255)
                          tatTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAAAGAACAGGATCCACACCACTggcgcggc                                                                           <  1:2320801/100‑1 (MQ=255)
                          natTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTggcgcggc                                                                           >  2:1612077/2‑100 (MQ=255)
                           atacGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGTCATTTCAGCGGCTGAACTTCAGAGCACAGGATCCACACCACTggcgcggcg                                                                          <  1:1159729/97‑1 (MQ=255)
                           atTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTggcgcggcg                                                                          <  2:3059822/100‑1 (MQ=255)
                              cGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCCCTGGCGCGGCGCTc                                                                       >  2:1985650/1‑100 (MQ=255)
                               gACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCa                                                                      <  2:1213046/100‑1 (MQ=255)
                               gACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCa                                                                      >  2:2770187/1‑100 (MQ=255)
                               gACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCa                                                                      >  1:72986/1‑100 (MQ=255)
                                  acCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCg                                                                   <  2:3796001/100‑1 (MQ=255)
                                   cccAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGc                                                                  >  2:1257711/1‑100 (MQ=255)
                                   cccAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGc                                                                  >  1:9684/1‑100 (MQ=255)
                                    ccAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCa                                                                 >  2:3037359/1‑100 (MQ=255)
                                     cAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCAt                                                                <  2:1442782/100‑1 (MQ=255)
                                     cAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCAt                                                                >  1:2521732/1‑100 (MQ=255)
                                      aGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGCGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCAt                                                                >  2:2701624/1‑99 (MQ=255)
                                            tggtggTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGc                                                         <  2:391949/100‑1 (MQ=255)
                                              gtggtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCaa                                                       <  1:305074/100‑1 (MQ=255)
                                              gtggtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCaa                                                       >  2:1888432/1‑100 (MQ=255)
                                               tggtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGAACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCaa                                                       >  2:2486413/1‑99 (MQ=255)
                                                ggtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATa                                                     >  2:4228430/1‑100 (MQ=255)
                                                ggtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATa                                                     >  2:227993/1‑100 (MQ=255)
                                                ggtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCCCATCGCATAACCGGCAATa                                                     <  1:1774126/100‑1 (MQ=255)
                                                   cGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATacac                                                  >  2:2518115/1‑100 (MQ=255)
                                                         ggTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACatcatc                                            >  2:2457344/1‑100 (MQ=255)
                                                         ggTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACatcatc                                            >  1:4095992/1‑100 (MQ=255)
                                                         ggTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGCCAATACACatcatc                                            >  1:499405/1‑100 (MQ=255)
                                                          gTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCg                                           <  2:3521114/100‑1 (MQ=255)
                                                          gTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCg                                           >  1:319367/1‑100 (MQ=255)
                                                          gTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCg                                           <  2:1085082/100‑1 (MQ=255)
                                                           tAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGt                                          >  2:1539240/1‑100 (MQ=255)
                                                           tAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGt                                          >  2:847612/1‑100 (MQ=255)
                                                           tAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGt                                          >  2:3509580/1‑100 (MQ=255)
                                                           tAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGt                                          <  2:2685228/100‑1 (MQ=255)
                                                              tACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCAt                                       >  1:2060856/1‑100 (MQ=255)
                                                               aCCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCa                                        <  1:1863567/98‑1 (MQ=255)
                                                                ncGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACACCATCGTCa                                        >  1:2089347/2‑97 (MQ=255)
                                                                ccGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGc                                     <  2:2784026/100‑1 (MQ=255)
                                                                acGAAATCGCGGCATTTCAGCGGCTGAATTTCACCGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGc                                     >  1:1542292/2‑100 (MQ=255)
                                                                 cGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGcc                                    <  1:3768466/100‑1 (MQ=255)
                                                                 cGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGACGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGcc                                    <  2:4183680/100‑1 (MQ=255)
                                                                  gcgaTCGCGGCATTTCTGCGGCTGAATATCAGAGCACAAGAGCCACACCACTGGCGCGGCTCTCATCGCATAACTCGCAATACACATCATCGTCATGCCa                                   >  1:13050/4‑100 (MQ=255)
                                                                  gAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGcc                                    <  1:1256127/99‑1 (MQ=255)
                                                                  gAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCa                                   <  2:1138254/100‑1 (MQ=255)
                                                                      tCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACag                               <  1:2518108/100‑1 (MQ=255)
                                                                       cgcgGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACaga                              >  1:2013002/1‑100 (MQ=255)
                                                                       cgcgGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACa                                <  1:1985646/98‑1 (MQ=255)
                                                                       cgcgGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACAACACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACaga                              >  1:1672293/1‑100 (MQ=255)
                                                                        gcgGCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACagag                             >  2:488260/1‑100 (MQ=255)
                                                                         cgGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACagaga                            <  1:397892/100‑1 (MQ=255)
                                                                          ggCATTTCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACagagag                           >  2:1447178/1‑100 (MQ=255)
                                                                          ggCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACagagag                           <  2:1845184/100‑1 (MQ=255)
                                                                          ggCATTTCAGCGGCTGAATTGCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACagagag                           <  2:924728/100‑1 (MQ=255)
                                                                           gCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGc                          <  1:1643014/100‑1 (MQ=255)
                                                                              tttCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAg                       <  2:2016001/100‑1 (MQ=255)
                                                                               ttCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAgg                      <  2:3743613/100‑1 (MQ=255)
                                                                               ttCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAgg                      <  2:3778086/100‑1 (MQ=255)
                                                                                tCAGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGa                     <  1:184235/100‑1 (MQ=255)
                                                                                tCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAgg                      <  2:189917/99‑1 (MQ=255)
                                                                                  aGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATACCCGGCCATACACATCATCGGCATGCCAACAGAGAGCCAGGAc                    >  1:2694614/1‑99 (MQ=255)
                                                                                   gCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACaa                  >  2:2265490/1‑100 (MQ=255)
                                                                                    cGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAg                 <  1:1631375/100‑1 (MQ=255)
                                                                                    cGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAg                 >  2:725660/1‑100 (MQ=255)
                                                                                    cGGCTGAATGTCAGAGCACAGGTACCAGACCACTAGCGCGGCGCTCATCGCATAACCCGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACaaa                 <  1:3727516/100‑2 (MQ=255)
                                                                                      gCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGcc               <  2:131517/100‑1 (MQ=255)
                                                                                      gCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGcc               <  2:2808385/100‑1 (MQ=255)
                                                                                      gCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGcc               <  1:327465/100‑1 (MQ=255)
                                                                                        tGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCAc             <  2:504998/100‑1 (MQ=255)
                                                                                           aTTTCAGATCACGGGATCCACACAACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGACCACAGACAGCCAGGACAAGCCACTgg          >  1:1479968/1‑100 (MQ=255)
                                                                                           aTTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTgg          >  1:175075/1‑100 (MQ=255)
                                                                                           aTTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTgg          >  2:314224/1‑100 (MQ=255)
                                                                                           aTTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTgg          >  2:3786040/1‑100 (MQ=255)
                                                                                           aTTTCAGAGCACAAGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTgg          <  2:3398256/100‑1 (MQ=255)
                                                                                             ttCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAATCCACTGGaa        <  1:423153/100‑1 (MQ=255)
                                                                                               cAGATCACAGGATCCACAACACTGCCGCCGCGCTCATCTGTTAACCGGCAATACACATCACCGTCATGCCAACAGAGAGCCGGGACAAGCCACTGGAAGc      <  1:419892/100‑1 (MQ=255)
                                                                                                agagCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGcc     >  1:2721699/1‑100 (MQ=255)
                                                                                                agagCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGcc     <  1:3963614/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTA  >  NC_000913/2980932‑2981130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑