Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 475,614 A→G 44.8% D79G (GAT→GGT)  ybaY → outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913475,6140AG44.8% 23.1 / 196.3 152D79G (GAT→GGT) ybaYouter membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (36/48);  new base G (33/35);  total (69/83)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-02

Only 100 of 153 total aligned reads displayed.

CAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCC  >  NC_000913/475517‑475713
                                                                                                 |                                                                                                   
cAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGAt                                                                                                    <  1:3264543/99‑1 (MQ=255)
cAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGCTGGCCGATg                                                                                                   >  1:3791906/1‑100 (MQ=255)
 aaCCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGc                                                                                                  <  1:1293891/100‑1 (MQ=255)
   ccGTATGTCTCCGGTACCGGCTGTATCCGTGAGAAAGTCGCACTGCCGCCTGATGCGGTGCTGACCGTGACACTTGCTGACGCGTCGTTAGCCGATGCAc                                                                                                >  1:3840875/1‑100 (MQ=255)
        tGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCa                                                                                           <  1:1544453/100‑1 (MQ=255)
         gTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCa                                                                                           >  2:340885/1‑99 (MQ=255)
         gTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCaa                                                                                          >  1:2431438/1‑100 (MQ=255)
          tctcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCaaa                                                                                         >  1:3365578/1‑100 (MQ=255)
            tcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAg                                                                                       >  2:1354048/1‑100 (MQ=255)
               ggTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGc                                                                                    >  1:3125317/1‑100 (MQ=255)
               ggTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTgn                                                                                    <  2:1381625/100‑2 (MQ=255)
               ggTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGc                                                                                    <  1:542737/100‑1 (MQ=255)
                gTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCt                                                                                   <  2:1433824/100‑1 (MQ=255)
                gTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCt                                                                                   <  2:1612767/100‑1 (MQ=255)
                 tACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTg                                                                                  <  2:1199044/100‑1 (MQ=255)
                      tcgggATCCGTCAAAAAGTCGCACTGCCGCTTGATGCTGTGCTGACCGTGACACTTGCTGACGCGTCGTGAGCCGGTGCACCGTCAAAAGTGCTGGCGCa                                                                             <  1:876161/97‑1 (MQ=255)
                      tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCa                                                                             <  2:1307907/100‑1 (MQ=255)
                       cTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAg                                                                            <  2:827683/100‑1 (MQ=255)
                         ggATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAgn                                                                           <  2:2050545/99‑2 (MQ=255)
                         ggATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGaa                                                                          >  1:198619/1‑100 (MQ=255)
                          gATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGaaa                                                                         >  2:1251707/1‑100 (MQ=255)
                           aTCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAg                                                                        >  2:2055376/1‑100 (MQ=255)
                           aTCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAg                                                                        >  1:619841/1‑100 (MQ=255)
                            tCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGc                                                                       <  2:1642791/100‑1 (MQ=255)
                            tCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACCTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGc                                                                       >  1:1571633/1‑100 (MQ=255)
                             ccGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCg                                                                      >  1:1045135/1‑100 (MQ=255)
                              cGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGCCGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCgg                                                                     >  2:415650/1‑100 (MQ=255)
                              cGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCgg                                                                     <  1:560835/100‑1 (MQ=255)
                               gTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGt                                                                    <  2:1878824/100‑1 (MQ=255)
                                  aGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCg                                                                 <  1:1073973/100‑1 (MQ=255)
                                  aGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCg                                                                 >  1:20939/1‑100 (MQ=255)
                                  aGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCg                                                                 <  2:1581297/100‑1 (MQ=255)
                                  aGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCg                                                                 <  2:38321/100‑1 (MQ=255)
                                  aGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCg                                                                 >  2:3330965/1‑100 (MQ=255)
                                   naaaGTCGCACTGCCGCCTGNTGCTGTGCTGACCGTGACACTTTCTGACNCGTCGTTAGCCGATGCACCGCCANAAGTGCTNGCNCAGAAAGCGGTGCg                                                                 >  1:905649/2‑99 (MQ=255)
                                   gAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGt                                                                >  1:3211460/1‑100 (MQ=255)
                                     aaGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGCCGCGTCGTTAGCCGGTGCACCGTAAAAACTGCTGGCGCAGAAAGCGGTGCGTa                                                               >  1:406822/1‑99 (MQ=255)
                                     aaGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTAc                                                              >  1:2702463/1‑100 (MQ=255)
                                     aaGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTAc                                                              >  1:3111914/1‑100 (MQ=255)
                                     aaGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGAGGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTAc                                                              <  1:3009500/100‑1 (MQ=255)
                                      aGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACt                                                             <  2:2542161/100‑1 (MQ=255)
                                       gTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTAc                                                              <  1:619582/98‑1 (MQ=255)
                                       gTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTg                                                            >  2:1576286/1‑100 (MQ=255)
                                       gTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTg                                                            <  1:3722823/100‑1 (MQ=255)
                                        tCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGa                                                           >  1:2549326/1‑100 (MQ=255)
                                         cGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGaa                                                          >  2:327891/1‑100 (MQ=255)
                                         cGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGaa                                                          >  1:2919884/1‑100 (MQ=255)
                                             cTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTa                                                      <  2:3851165/100‑1 (MQ=255)
                                             cTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTa                                                      <  2:2688850/100‑1 (MQ=255)
                                                ccgccTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAAc                                                   <  1:2208064/100‑1 (MQ=255)
                                                   ccTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGt                                                <  2:3075313/100‑1 (MQ=255)
                                                      gATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCAc                                             <  2:497366/100‑1 (MQ=255)
                                                       gatctgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCAcc                                            <  2:2200600/97‑1 (MQ=255)
                                                       aTGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCAcc                                            <  2:2381291/100‑1 (MQ=255)
                                                        tgctgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCa                                           <  2:3532952/100‑1 (MQ=255)
                                                        tgctgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCa                                           >  2:4104377/1‑100 (MQ=255)
                                                         gctgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCAt                                          <  2:3704646/100‑1 (MQ=255)
                                                           tgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCTGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTc                                        <  1:2285923/100‑1 (MQ=255)
                                                           tgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTc                                        <  1:447551/100‑1 (MQ=255)
                                                           tgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTc                                        >  1:4135835/1‑100 (MQ=255)
                                                            gtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCa                                       >  2:3626054/1‑100 (MQ=255)
                                                             tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAg                                      >  2:4142405/1‑100 (MQ=255)
                                                              gctgACCGTTACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAATGTAAACAGTCACCATTCAg                                      <  1:387151/99‑1 (MQ=255)
                                                              gctgACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGc                                     >  2:744194/1‑100 (MQ=255)
                                                                tgACCGTGACACTGTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGGGCGTACTGAAGGTAAACAGTCACCATGCAGCtt                                   >  1:3840838/1‑100 (MQ=255)
                                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCttt                                  >  2:1823815/1‑100 (MQ=255)
                                                                  aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTg                                 <  1:3832017/100‑1 (MQ=255)
                                                                  aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTg                                 <  1:3339503/100‑1 (MQ=255)
                                                                     gTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTc                              <  1:932845/100‑1 (MQ=255)
                                                                     gTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTc                              <  1:145919/100‑1 (MQ=255)
                                                                     gTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTc                              <  1:1850557/100‑1 (MQ=255)
                                                                         cacTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTc                          >  1:392324/1‑100 (MQ=255)
                                                                          acTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCa                         <  1:680433/100‑1 (MQ=255)
                                                                          acTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCa                         <  1:3160453/100‑1 (MQ=255)
                                                                          acTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCa                         <  1:1122242/100‑1 (MQ=255)
                                                                            tttCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCAtt                       >  2:93980/1‑100 (MQ=255)
                                                                             ttCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCAttt                      <  1:1898619/100‑1 (MQ=255)
                                                                              tCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTa                     <  1:4266460/100‑1 (MQ=255)
                                                                               cTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTaa                    >  2:3141966/1‑100 (MQ=255)
                                                                               cTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTaa                    >  2:4089963/1‑100 (MQ=255)
                                                                                tGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAAc                   <  2:1329302/100‑1 (MQ=255)
                                                                                tGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAAc                   <  2:1471204/100‑1 (MQ=255)
                                                                                 gACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAAcc                  >  1:249133/1‑100 (MQ=255)
                                                                                   cgcgTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCg                >  2:3601183/1‑100 (MQ=255)
                                                                                     cgtcgtTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc              <  1:2636815/100‑1 (MQ=255)
                                                                                      gtcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCa             <  1:3578431/100‑1 (MQ=255)
                                                                                      ggcgtTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc              <  1:104661/97‑1 (MQ=255)
                                                                                        cgtTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGa           >  1:365752/1‑100 (MQ=255)
                                                                                         gtTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGAt          >  1:1949913/1‑100 (MQ=255)
                                                                                         gtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGAt          <  2:1072403/100‑1 (MQ=255)
                                                                                          ttAGCCGATGCACCGTCAAAAGTGCTGGCTCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTTTTCTGTCATTTAACCCGGCAGATg         <  1:715248/100‑1 (MQ=255)
                                                                                          ttAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATg         <  2:1936012/100‑1 (MQ=255)
                                                                                           tAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGt        <  2:2338593/100‑1 (MQ=255)
                                                                                            aGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGtt       >  1:2110732/1‑100 (MQ=255)
                                                                                            aGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGtt       >  2:2594907/1‑100 (MQ=255)
                                                                                             gCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTc      >  1:2923144/1‑100 (MQ=255)
                                                                                               cGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCa     <  2:4269426/99‑1 (MQ=255)
                                                                                               cGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAg    <  2:3949576/100‑1 (MQ=255)
                                                                                               cGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAg    <  2:2096535/100‑1 (MQ=255)
                                                                                                 aTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGcc  <  2:1111445/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
CAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCC  >  NC_000913/475517‑475713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: