Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,244,987 C→T 52.2% intergenic (‑246/‑237) uxaC ← / → exuT uronate isomerase/hexuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,244,9870CT52.2% 15.0 / 245.6 179intergenic (‑246/‑237)uxaC/exuTuronate isomerase/hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base C (42/43);  new base T (56/37);  total (99/80)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGA  >  NC_000913/3244889‑3245086
                                                                                                  |                                                                                                   
 cTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATaa                                                                                                   >  1:1457629/1‑100 (MQ=255)
 cTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCACTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACaa                                                                                                   >  1:2411903/1‑100 (MQ=255)
  tGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAg                                                                                                  >  2:941334/1‑100 (MQ=255)
  tGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAg                                                                                                  <  1:782468/100‑1 (MQ=255)
  tGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAg                                                                                                  >  1:2083631/1‑100 (MQ=255)
   gCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt                                                                                                 <  2:3075914/100‑1 (MQ=255)
       gCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATc                                                                                             <  1:4011818/100‑1 (MQ=255)
        ccGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCt                                                                                            <  2:1099022/100‑1 (MQ=255)
        ccGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCt                                                                                            <  2:506798/100‑1 (MQ=255)
         cgcgCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCtt                                                                                           >  2:312311/1‑100 (MQ=255)
         cgcgCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCtt                                                                                           >  2:2515510/1‑100 (MQ=255)
           cgttagcaCTGGCCTTGCTGGCGGCAGTTAATGGCCCTACAAATATGCCCACATTGGTGATGGCTGTCACCTTTTAAAGTTGTATGACAATTTa                                                                                               <  1:4080483/87‑1 (MQ=255)
            gcCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCt                                                                                        <  2:410132/100‑1 (MQ=255)
              cAGTACTGGCCTTGCTGTCTTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTg                                                                                       <  1:549076/99‑1 (MQ=255)
                 tACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGt                                                                                   >  1:569103/1‑100 (MQ=255)
                  aCTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTc                                                                                  >  1:481548/1‑100 (MQ=255)
                  aCTGGCCGTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCg                                                                                    <  1:2976113/98‑1 (MQ=255)
                   cTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCg                                                                                 <  1:930887/100‑1 (MQ=255)
                     ggCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCa                                                                               >  1:3475725/1‑100 (MQ=255)
                     ggCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCa                                                                               >  2:2030103/1‑100 (MQ=255)
                     ggCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCa                                                                               >  2:4086718/1‑100 (MQ=255)
                      gCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCaa                                                                              >  1:1814669/1‑100 (MQ=255)
                         ttGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAAt                                                                            <  2:2927358/99‑1 (MQ=255)
                         ttGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAAt                                                                            <  1:3013923/99‑1 (MQ=255)
                         ttGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATc                                                                           >  1:4096328/1‑100 (MQ=255)
                         ttGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATc                                                                           <  2:3112900/100‑1 (MQ=255)
                         ttGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATc                                                                           >  2:2621549/1‑100 (MQ=255)
                          tGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCa                                                                          <  2:2100964/100‑1 (MQ=255)
                          tGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCa                                                                          >  2:2856946/1‑100 (MQ=255)
                          tGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa                                                                          >  2:1201389/1‑100 (MQ=255)
                          tGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa                                                                          <  2:1437113/100‑1 (MQ=255)
                           gCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCAt                                                                         >  2:3653261/1‑100 (MQ=255)
                           gCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCAt                                                                         >  2:2072855/1‑100 (MQ=255)
                            cTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATa                                                                        <  1:2854733/100‑1 (MQ=255)
                             tGTCGTCAGGTAATGTCCCTACAATTATTCCCACATGTGTGATGGCTCTCACATTTTAAAGTCGTATGATAAGTTATCTTTCTATCGTCGCAAATCATaa                                                                       >  1:3891149/1‑100 (MQ=255)
                              gtcgtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAg                                                                      >  2:625301/1‑100 (MQ=255)
                               tcgtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGt                                                                     <  1:1232769/100‑1 (MQ=255)
                                cgtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTc                                                                    <  2:1028046/100‑1 (MQ=255)
                                 gtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCg                                                                   >  2:2368741/1‑100 (MQ=255)
                                 gtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCg                                                                   >  2:1224393/1‑100 (MQ=255)
                                  tcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGa                                                                  >  2:3446671/1‑100 (MQ=255)
                                   cAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAc                                                                 <  1:1087634/100‑1 (MQ=255)
                                     ggTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACgg                                                               >  2:3267866/1‑100 (MQ=255)
                                        aaTGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAAt                                                            <  1:3291915/100‑1 (MQ=255)
                                        aaTGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAAt                                                            <  2:3049736/100‑1 (MQ=255)
                                           gTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCa                                                         >  2:3963500/1‑100 (MQ=255)
                                             cccTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCaaa                                                       <  2:3029646/100‑1 (MQ=255)
                                             cccTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCaaa                                                       <  2:3323056/100‑1 (MQ=255)
                                               cTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAAtt                                                     >  2:1056622/1‑100 (MQ=255)
                                               cTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAAtt                                                     >  1:3512164/1‑100 (MQ=255)
                                                tACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTg                                                    >  1:1877146/1‑100 (MQ=255)
                                                tACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTg                                                    <  2:844979/100‑1 (MQ=255)
                                                tACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTg                                                    >  2:599376/1‑100 (MQ=255)
                                                  cAAATATTCCTACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAGATCATAAGTCGACGGAATGCAAATTGcc                                                  <  2:2062739/100‑1 (MQ=255)
                                                  cAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAATTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGc                                                   >  1:1609984/1‑99 (MQ=255)
                                                  cAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGcc                                                  <  1:1528723/100‑1 (MQ=255)
                                                   naaTATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCCTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCg                                                 >  1:2726488/2‑100 (MQ=255)
                                                     atatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGat                                               <  2:1637758/100‑1 (MQ=255)
                                                      tatTCGTACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCTACGGAATGCAAATTGCCg                                                 <  1:2521542/97‑1 (MQ=255)
                                                      tatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGatt                                              <  2:3900600/100‑1 (MQ=255)
                                                      tatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGatt                                              <  2:1908071/100‑1 (MQ=255)
                                                      tatTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGatt                                              <  1:2248460/100‑1 (MQ=255)
                                                       atTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattc                                             >  2:1568925/1‑100 (MQ=255)
                                                        ttCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattca                                            <  1:887106/100‑1 (MQ=255)
                                                          cgcacaTTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcatt                                          >  2:2898081/3‑100 (MQ=255)
                                                          cccACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCGAATTGCCGattcatt                                          <  1:669007/100‑1 (MQ=255)
                                                          cccACATTTGTGATGGCTCTCACCTTTTAAAGGTGTATAATAAGTTATCTTTCTGCCGTCGCAAATAATAAGTCGACGGAATGCAAATTGCCGattcatt                                          >  2:573/1‑100 (MQ=255)
                                                           ccACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcattc                                         >  1:3710247/1‑100 (MQ=255)
                                                           ccACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcattc                                         >  2:3869947/1‑100 (MQ=255)
                                                            cacaTTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGAttcattca                                        >  1:3169588/1‑100 (MQ=255)
                                                                tttGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTg                                    <  1:3922050/100‑1 (MQ=255)
                                                                   gtgATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTa                                 <  2:1473478/100‑1 (MQ=255)
                                                                    tgATGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAg                                <  2:2042445/100‑1 (MQ=255)
                                                                     gATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGa                               >  1:4220395/1‑100 (MQ=255)
                                                                       tGGCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATg                             >  1:3019861/1‑100 (MQ=255)
                                                                        ggCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTGTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGa                            >  1:2625563/1‑100 (MQ=255)
                                                                         gCTCTCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGaa                           >  2:748916/1‑100 (MQ=255)
                                                                          ctctCACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAAt                          >  2:1854444/1‑100 (MQ=255)
                                                                          ctctCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGCCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAAt                          >  2:422668/1‑100 (MQ=255)
                                                                              cACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCggg                      <  2:2311076/100‑1 (MQ=255)
                                                                              cACCTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCggg                      >  2:1266705/1‑100 (MQ=255)
                                                                              cACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGCCGCAAATCATAAGTCGACGCAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCggg                      <  1:2321719/100‑1 (MQ=255)
                                                                                ccTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGtt                    >  2:1772631/1‑100 (MQ=255)
                                                                                 cTTTTAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTa                   >  1:3104796/1‑100 (MQ=255)
                                                                                   tttAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAAc                 <  2:380958/100‑1 (MQ=255)
                                                                                   tttAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAAc                 <  1:103193/100‑1 (MQ=255)
                                                                                    ttAAAGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAAcc                >  1:3266947/1‑100 (MQ=255)
                                                                                       aaGTTGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGt             <  2:711017/100‑1 (MQ=255)
                                                                                       aaGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGt             <  2:3169600/100‑1 (MQ=255)
                                                                                       aaGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGt             >  1:200154/1‑100 (MQ=255)
                                                                                        aGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCTGTa            >  1:513447/1‑100 (MQ=255)
                                                                                        aGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTa            <  1:3637633/100‑1 (MQ=255)
                                                                                          ttGTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACg          <  2:3435835/100‑1 (MQ=255)
                                                                                           tGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACgg         <  2:250877/100‑1 (MQ=255)
                                                                                            gTATGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGa        >  1:218836/1‑100 (MQ=255)
                                                                                             tATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTTGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGaa       >  1:2633283/1‑100 (MQ=255)
                                                                                              aTGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAg      >  2:732952/1‑100 (MQ=255)
                                                                                               tGATAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGc     >  2:3548914/1‑100 (MQ=255)
                                                                                                gACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGcc    <  2:1722875/100‑1 (MQ=255)
                                                                                                 aCAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCg   >  2:2929665/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGA  >  NC_000913/3244889‑3245086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: