Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,267,608 C→T 18.0% intergenic (+10/‑246) tdcR → / → yhaB L‑threonine dehydratase operon activator protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,267,6080CT18.0% 358.6 / 59.7 159intergenic (+10/‑246)tdcR/yhaBL‑threonine dehydratase operon activator protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (67/61);  new base T (16/13);  total (85/74)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 168 total aligned reads displayed.

ACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTT  >  NC_000913/3267509‑3267706
                                                                                                   |                                                                                                  
aCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTcc                                                                                                    <  2:637013/100‑1 (MQ=255)
  aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGGCGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTcccc                                                                                                  <  2:1364856/100‑1 (MQ=255)
  aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTcccc                                                                                                  >  2:1269113/1‑100 (MQ=255)
    gTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTc                                                                                                >  1:3066883/1‑100 (MQ=255)
    gTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTc                                                                                                <  2:2275804/100‑1 (MQ=255)
     tATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCa                                                                                               <  2:4179518/100‑1 (MQ=255)
       ttGATCATCTCGAACAATTGATTAACGTCAACTTTTTTTTTTTTTACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATa                                                                                             >  1:4217452/1‑100 (MQ=255)
        tGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAg                                                                                            >  2:1101038/1‑100 (MQ=255)
         gATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGCATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAgg                                                                                           >  1:87102/1‑100 (MQ=255)
         gATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAgg                                                                                           >  1:3155831/1‑100 (MQ=255)
         gATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAgg                                                                                           <  2:2124651/100‑1 (MQ=255)
          atcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGc                                                                                          >  2:2499808/1‑100 (MQ=255)
          atcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGc                                                                                          >  2:3925835/1‑100 (MQ=255)
             atcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGcgac                                                                                       <  1:1308149/100‑1 (MQ=255)
             atcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGcgac                                                                                       <  2:772195/100‑1 (MQ=255)
               ctcGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGcgac                                                                                       >  1:200114/1‑98 (MQ=255)
               ctcGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAAGGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGcgacca                                                                                     <  2:2888766/100‑3 (MQ=255)
                tcGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGaa                                                                                    >  1:3986638/1‑100 (MQ=255)
                tcGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGaa                                                                                    >  1:2503927/1‑100 (MQ=255)
                 cGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAAt                                                                                   >  2:1827544/1‑100 (MQ=255)
                 cGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAAt                                                                                   >  2:2913540/1‑100 (MQ=255)
                 cGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAAt                                                                                   <  1:2745523/100‑1 (MQ=255)
                 cGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAAt                                                                                   >  1:2436863/1‑100 (MQ=255)
                    aCAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGc                                                                                <  2:2559440/100‑1 (MQ=255)
                    aCAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGc                                                                                >  2:4260701/1‑100 (MQ=255)
                     cAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCa                                                                               <  2:7676/100‑1 (MQ=255)
                          gATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTtg                                                                          >  1:652819/1‑100 (MQ=255)
                             tAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTgtgt                                                                       <  2:583236/100‑1 (MQ=255)
                              aaCGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGtt                                                                      >  2:3197764/1‑100 (MQ=255)
                              aaCGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGtt                                                                      <  1:2429856/100‑1 (MQ=255)
                              aaCGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGtt                                                                      >  2:3736573/1‑100 (MQ=255)
                               aCGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTg                                                                     <  2:3198043/100‑1 (MQ=255)
                                cGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGa                                                                    >  2:2668529/1‑100 (MQ=255)
                                cGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGa                                                                    >  2:2424962/1‑100 (MQ=255)
                                 gTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAg                                                                   >  1:2169634/1‑100 (MQ=255)
                                  tCAACTTTTTCTCTTCTTACAGGACGTCATTTTGTGAATGCAGTCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGtt                                                                      <  1:2504758/96‑1 (MQ=255)
                                  tCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAGTCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAgg                                                                  <  1:3002446/100‑1 (MQ=255)
                                   cAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAg                                                                   >  1:1040467/1‑98 (MQ=255)
                                   cAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGa                                                                 >  2:1164170/1‑100 (MQ=255)
                                   cAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGa                                                                 <  2:879674/100‑1 (MQ=255)
                                   cAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGa                                                                 <  2:934371/100‑1 (MQ=255)
                                   cAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGa                                                                 <  1:1126996/100‑1 (MQ=255)
                                     aCTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCGTAGGCGACGAATAGCATTTTGTGTTGAGGATc                                                               <  2:4057041/100‑1 (MQ=255)
                                     aCTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATc                                                               >  2:1374673/1‑100 (MQ=255)
                                     aCTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATc                                                               <  1:851856/100‑1 (MQ=255)
                                     aCTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATc                                                               <  1:1963917/100‑1 (MQ=255)
                                       tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATcac                                                             <  1:282354/100‑1 (MQ=255)
                                       tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATcac                                                             <  1:282368/100‑1 (MQ=255)
                                       tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATcac                                                             <  1:3645099/100‑1 (MQ=255)
                                       tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATcac                                                             <  1:3047532/100‑1 (MQ=255)
                                       tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATcac                                                             <  1:3678192/100‑1 (MQ=255)
                                        ttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGTCGACGAATAGCATTTTGTGTTGAGGATca                                                              >  2:2064757/1‑98 (MQ=255)
                                         tttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACaa                                                           >  1:4009521/1‑100 (MQ=255)
                                          ttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATcaca                                                            >  1:4032710/1‑98 (MQ=255)
                                           tctcTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACaaag                                                         >  2:3027864/1‑99 (MQ=255)
                                            ctctTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAAc                                                        >  2:2912899/1‑100 (MQ=255)
                                            ctctTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAAc                                                        >  1:3438509/1‑100 (MQ=255)
                                            ctctTCTGACAGGACGTCATTTTGTGAATGCAATAGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGGTGAGGATCACAAAAc                                                        >  2:4202329/1‑100 (MQ=255)
                                                tctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGaat                                                    >  1:3762716/1‑100 (MQ=255)
                                                tctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGaat                                                    >  2:3316435/1‑100 (MQ=255)
                                                tctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGaat                                                    >  1:2262826/1‑100 (MQ=255)
                                                 ctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGaata                                                   >  2:801803/1‑100 (MQ=255)
                                                 ctGACAGGACGTCATTTTGTGAATGCAATCGTATTCCATAAATTCTTCTCACCTCATAGGCCACGAATAGCATTTTATGTTGAGAATCACAAAAAGcata                                                   >  2:4061178/1‑100 (MQ=255)
                                                      aGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGc                                              >  2:1571753/1‑100 (MQ=255)
                                                        gACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTg                                            <  1:4255457/100‑1 (MQ=255)
                                                        gACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTg                                            <  1:10824/100‑1 (MQ=255)
                                                        gACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTg                                            <  1:954429/100‑1 (MQ=255)
                                                          cGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGAt                                          >  1:674057/1‑100 (MQ=255)
                                                          cGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGAt                                          >  1:3022101/1‑100 (MQ=255)
                                                           gTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATc                                         >  2:673166/1‑100 (MQ=255)
                                                             cATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATcgc                                       >  2:2422956/1‑100 (MQ=255)
                                                               ttttGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATcgccg                                     <  2:2784658/100‑1 (MQ=255)
                                                                tttGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATcgccgc                                    >  2:811931/1‑100 (MQ=255)
                                                                tttGTGAATGCAATCGTTTTCCATAAATTCTTCTCTCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATcgccgc                                    <  1:3923749/100‑1 (MQ=255)
                                                                  tgtgAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGa                                  >  1:7941/1‑100 (MQ=255)
                                                                   gtgAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGAt                                 <  1:1144670/100‑1 (MQ=255)
                                                                   gtgAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGAt                                 <  1:2365894/100‑1 (MQ=255)
                                                                     gAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATaa                               <  1:1051707/100‑1 (MQ=255)
                                                                     gAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATaa                               >  2:4199498/1‑100 (MQ=255)
                                                                       aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAgn                             <  2:3601717/100‑2 (MQ=255)
                                                                       aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAgg                             <  1:826301/100‑1 (MQ=255)
                                                                        tGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGt                            <  1:1434427/100‑1 (MQ=255)
                                                                          cAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCa                          <  2:3094997/100‑1 (MQ=255)
                                                                             taattttCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGAACACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagac                       <  1:2447346/97‑1 (MQ=255)
                                                                               gTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGCTCACAGAACGAATAATTGCTCATCGACGCGATACGGACagacaa                     >  1:2414621/1‑100 (MQ=255)
                                                                                ttttCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaa                    <  2:3693529/100‑1 (MQ=255)
                                                                                 tttCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaag                   <  2:3762733/100‑1 (MQ=255)
                                                                                 tttCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaag                   <  2:1487691/100‑1 (MQ=255)
                                                                                 tttCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaag                   <  1:4057645/100‑1 (MQ=255)
                                                                                   tCCATAAATTCCTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGGTCGCCGCGATAAGGTCagacaaagac                 >  1:1698917/1‑100 (MQ=255)
                                                                                     cATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaagacaa               >  1:173437/1‑100 (MQ=255)
                                                                                     cATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaagacaa               >  2:1041758/1‑100 (MQ=255)
                                                                                      aTAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGacaac              >  1:3248158/1‑100 (MQ=255)
                                                                                        aaaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGAcaacaa            <  1:3487792/100‑1 (MQ=255)
                                                                                          aTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAgg          >  2:572202/1‑100 (MQ=255)
                                                                                          aTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAgg          <  2:1582941/100‑1 (MQ=255)
                                                                                          aTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAgg          <  2:1689219/100‑1 (MQ=255)
                                                                                                ctctCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAAtt    <  2:3486966/100‑1 (MQ=255)
                                                                                                ctcCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAAtt    <  1:4260678/100‑1 (MQ=255)
                                                                                                  ccccTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTACTGATCGCCGCGATAAGGTCAGACAAAGACAAAAAGGaaaa      >  2:2485611/1‑96 (MQ=255)
                                                                                                   |                                                                                                  
ACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTT  >  NC_000913/3267509‑3267706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: