Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,410,196 T→A 24.5% intergenic (+245/‑84) prmA → / → dusB methyltransferase for 50S ribosomal subunit protein L11/tRNA‑dihydrouridine synthase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,410,1960TA24.5% 323.9 / 108.7 167intergenic (+245/‑84)prmA/dusBmethyltransferase for 50S ribosomal subunit protein L11/tRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base T (45/81);  new base A (25/16);  total (70/97)
Fisher's exact test for biased strand distribution p-value = 6.06e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 172 total aligned reads displayed.

TGTAAATTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAA  >  NC_000913/3410097‑3410294
                                                                                                    |                                                                                                  
tgtAAATTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGAtt                                                                                                    <  2:2201636/100‑1 (MQ=255)
 gtAAATTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGAtt                                                                                                    >  1:3078425/1‑99 (MQ=255)
  tAAATTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTgg                                                                                                  <  2:601554/100‑1 (MQ=255)
  tAAATTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTgg                                                                                                  <  1:3811208/100‑1 (MQ=255)
   aaaTTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGt                                                                                                 <  2:1972685/100‑1 (MQ=255)
        ttAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCACTTCTTTGATCCATCTCAGAGGATTGGTCAAAg                                                                                            <  2:4049331/100‑1 (MQ=255)
        ttAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAg                                                                                            <  1:1710634/100‑1 (MQ=255)
           gTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGttt                                                                                         >  1:570177/1‑100 (MQ=255)
            tCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTg                                                                                        <  2:104731/100‑1 (MQ=255)
            tCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTg                                                                                        <  1:413771/100‑1 (MQ=255)
             cACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTgg                                                                                       >  1:1291001/1‑100 (MQ=255)
             cACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTgg                                                                                       <  1:3927172/100‑1 (MQ=255)
              aCTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGc                                                                                      <  1:3760028/100‑1 (MQ=255)
              aCTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGc                                                                                      <  1:6577/100‑1 (MQ=255)
              aCTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGc                                                                                      >  2:1618915/1‑100 (MQ=255)
                  ttttCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAgatcggaagag                                                                                  >  1:4111715/1‑90 (MQ=255)
                    ttCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCa                                                                                <  1:1995433/100‑1 (MQ=255)
                    ttCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCa                                                                                <  2:3863287/100‑1 (MQ=255)
                      cTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAtc                                                                              >  2:3222724/1‑100 (MQ=255)
                          tatGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGt                                                                          <  1:654204/100‑1 (MQ=255)
                           atgatgATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGtc                                                                         >  2:3824186/1‑99 (MQ=255)
                           atgatgATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTg                                                                         <  2:1957902/100‑1 (MQ=255)
                            tgatgaTTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGc                                                                        <  2:1706902/100‑1 (MQ=255)
                              atgatTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCCGAGGATTGGTCAAAGTTTGCCCTTTCATCTCGTGCaa                                                                      >  2:47747/1‑100 (MQ=255)
                              atgatTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCaa                                                                      >  2:360337/1‑100 (MQ=255)
                               tgatTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCaaa                                                                     >  2:1977583/1‑100 (MQ=255)
                                gatTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCaaaa                                                                    <  2:3572379/100‑1 (MQ=255)
                                      tATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCg                                                              <  2:1273292/100‑1 (MQ=255)
                                      tATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCg                                                              <  2:256921/100‑1 (MQ=255)
                                       aTGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGt                                                             >  1:487137/1‑100 (MQ=255)
                                       aTGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGt                                                             >  2:806482/1‑100 (MQ=255)
                                        tGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTa                                                            <  2:413043/100‑1 (MQ=255)
                                         gAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGTGGATTCGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTa                                                            >  2:1500054/1‑99 (MQ=255)
                                         gAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTaa                                                           >  2:2960784/1‑100 (MQ=255)
                                         gAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTaa                                                           >  1:108436/1‑100 (MQ=255)
                                          aGTAATTATCGCACCACGCTCATTTTAAAGGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGCTTGGCCTTTCATCTCGTGCAAAAAATGCGTAat                                                          <  1:2568975/100‑1 (MQ=255)
                                          aGTAATTATCAGCCCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGACGATTGGCCAAAGTTTGGCCTTTCATCTCGTGCAAACAAt                                                                 <  1:2683821/93‑1 (MQ=255)
                                           gTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAata                                                         >  1:3236251/1‑100 (MQ=255)
                                            tAATTATCGCACCACGCTCATTTTAAATGAAATTCTTTGATACATCTCAGAGGATAGGTCAAAGTTTGGCATTACAAATAGAAAAAAAAATGCGTAata                                                         <  1:1575833/99‑1 (MQ=255)
                                             aactATCCCACCACGCTCAGTTTAAAATGCAATGCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAatat                                                        <  2:1419524/97‑1 (MQ=255)
                                              aTTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATAc                                                      >  2:2018382/1‑100 (MQ=255)
                                              aTTAGCGCAACACGGGGATTTTAAAAGAAATTCTTTGATCCAACTCAGAGGATAGGTCAAAGTTTGGCCTTTAAAAAAAAGAAAAAAATGCGTAAtata                                                       <  1:2117804/99‑1 (MQ=255)
                                               ttATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATAcg                                                     >  2:117248/1‑100 (MQ=255)
                                               ttATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATAcg                                                     <  2:3997911/100‑1 (MQ=255)
                                                 aTCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATAcgcc                                                   <  2:4045379/100‑1 (MQ=255)
                                                 aTCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATAcgcc                                                   <  1:280204/100‑1 (MQ=255)
                                                 aTCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATAcgc                                                    >  1:3513812/1‑99 (MQ=255)
                                                 aTCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATAcgcc                                                   >  1:3300053/1‑100 (MQ=255)
                                                 aTCGCACCACGATCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAACAAAAAAAAAAAATGCGTAATATAcgcc                                                   <  1:1855644/100‑1 (MQ=255)
                                                     caccacGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCt                                               <  2:2864824/100‑1 (MQ=255)
                                                     caccacGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCt                                               >  1:1237013/1‑100 (MQ=255)
                                                        cacGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGc                                            <  2:3497701/100‑1 (MQ=255)
                                                        cacGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGc                                            <  2:1683849/100‑1 (MQ=255)
                                                        cacGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGc                                            <  1:1941013/100‑1 (MQ=255)
                                                        cacGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGc                                            <  2:3210791/100‑1 (MQ=255)
                                                         acGCTCATTTTAAATGCAATTCTTTGATTCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGc                                            <  1:2334936/99‑1 (MQ=255)
                                                          cGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAg                                          >  1:4109626/1‑100 (MQ=255)
                                                           gCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGt                                         <  2:3913519/100‑1 (MQ=255)
                                                           gCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGt                                         <  2:2613724/100‑1 (MQ=255)
                                                           gCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGt                                         <  2:2934262/100‑1 (MQ=255)
                                                              cATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTcac                                      <  2:307407/100‑1 (MQ=255)
                                                              cATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTcac                                      >  2:3458000/1‑100 (MQ=255)
                                                              cATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTcac                                      >  2:1531037/1‑100 (MQ=255)
                                                              cATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTcac                                      <  2:2320244/100‑1 (MQ=255)
                                                               aTTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTcaca                                     >  1:2614695/1‑100 (MQ=255)
                                                                 ttttAAATGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAg                                    <  2:2953020/100‑1 (MQ=255)
                                                                   ttAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTa                                  <  2:3664861/100‑1 (MQ=255)
                                                                    tAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTAt                                 <  1:504086/100‑1 (MQ=255)
                                                                     aaaTGCAATTCTTCGATACAACTTAGAGTATTGGTCAAAGTTTGGCCTTACAAAAAAAAAAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATg                                <  1:4075457/100‑1 (MQ=255)
                                                                       aTGCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGt                              >  2:1240208/1‑100 (MQ=255)
                                                                        tGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTc                             <  1:2421415/100‑1 (MQ=255)
                                                                         gccaTTCTTTGATACATCTCACATGTTTAGTCAAAGTTTGGCCGTTCATCTCGTGCAAAAAATGCTTAATATACGTCGTCTTGCAGTCACAGTATGGt                              <  1:2484655/95‑1 (MQ=255)
                                                                         gCAATTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCa                            >  1:755593/1‑100 (MQ=255)
                                                                          cAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGAGTAATATACGCCGCCTTGCAGTCACAGTATgacc                             >  1:850929/1‑95 (MQ=255)
                                                                           aaTTCTTTGATCCATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCAtt                          >  1:2943762/1‑100 (MQ=255)
                                                                              tCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCt                       <  1:3853335/100‑1 (MQ=255)
                                                                               cTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTcct                      >  1:2595466/1‑98 (MQ=255)
                                                                               cTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCtt                      <  1:1696574/100‑1 (MQ=255)
                                                                                tttGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTa                     <  2:2459259/100‑1 (MQ=255)
                                                                                tttGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTa                     <  1:48660/100‑1 (MQ=255)
                                                                                tttGATCCATCTCAAAGGATTAGTCAAAGTTTGGCCTTAAAAAAAAAAAAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTc                        <  1:1618911/97‑1 (MQ=255)
                                                                                  tGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAAc                   <  2:3676782/100‑1 (MQ=255)
                                                                                  tGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAAc                   <  1:1007699/100‑1 (MQ=255)
                                                                                     tCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCa                <  2:3650163/100‑1 (MQ=255)
                                                                                     tCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCa                >  2:510852/1‑100 (MQ=255)
                                                                                      ccATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCAt               >  2:1007820/1‑100 (MQ=255)
                                                                                       cATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATg              >  1:3472343/1‑100 (MQ=255)
                                                                                       cATCTCAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATg              >  2:3977433/1‑100 (MQ=255)
                                                                                         tctcAGAGGATTGGTCAAAGTTTGGCCTTTCATCTTGTGCAAAAAATGCGTAATATACGGCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATgcg            <  2:2394173/100‑1 (MQ=255)
                                                                                         tctcAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATgcg            <  1:2001418/100‑1 (MQ=255)
                                                                                         tctcAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATgcg            <  1:2018378/100‑1 (MQ=255)
                                                                                           tcAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCa          >  2:1348997/1‑100 (MQ=255)
                                                                                            cAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCAt         <  1:1734581/100‑1 (MQ=255)
                                                                                            cAGAGGATTGGTCAAAGTTTGGACTTTAAAAAAAAGAAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATgcg            <  1:1731672/97‑1 (MQ=255)
                                                                                            cAGAGGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGAAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCAt         >  1:2033013/1‑100 (MQ=255)
                                                                                              gagGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCCTAACTCATGCGCATCg       >  1:2495582/1‑100 (MQ=255)
                                                                                              gagGATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCg       >  2:3748735/1‑100 (MQ=255)
                                                                                               agGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCgg      <  2:2258228/100‑1 (MQ=255)
                                                                                                ggATAGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCgg      >  1:1305941/1‑99 (MQ=255)
                                                                                                  aTTGGTCAAAGTTTGGCCTTTAAAATAAAAAAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACa   <  1:1532960/100‑1 (MQ=255)
                                                                                                    |                                                                                                  
TGTAAATTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAA  >  NC_000913/3410097‑3410294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: