Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,410,649 A→G 52.8% I124V (ATC→GTC)  dusB → tRNA‑dihydrouridine synthase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,410,6490AG52.8% 50.6 / 212.7 173I124V (ATC→GTC) dusBtRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base A (40/41);  new base G (45/46);  total (86/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 173 total aligned reads displayed.

CCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAA  >  NC_000913/3410552‑3410747
                                                                                                 |                                                                                                  
ccAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATc                                                                                                  >  2:193361/1‑100 (MQ=255)
 cAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATcc                                                                                                 <  1:3590412/100‑1 (MQ=255)
  aGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCt                                                                                                <  1:690784/100‑1 (MQ=255)
      tattGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTAcc                                                                                            >  2:1473538/1‑100 (MQ=255)
       attGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCg                                                                                           <  2:2769795/100‑1 (MQ=255)
        ttGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGa                                                                                          >  2:677083/1‑100 (MQ=255)
        ttGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGa                                                                                          >  1:522109/1‑100 (MQ=255)
          gATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAgg                                                                                        >  1:3133436/1‑100 (MQ=255)
            tatCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGgtc                                                                                      >  2:1663329/1‑100 (MQ=255)
            tatCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGgtc                                                                                      >  1:1109354/1‑100 (MQ=255)
             atCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGgtcg                                                                                     <  1:4264391/100‑1 (MQ=255)
             atCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGgtcg                                                                                     <  2:3979688/100‑1 (MQ=255)
             atCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGgtc                                                                                      >  1:3270584/1‑99 (MQ=255)
               cAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGgtcgtc                                                                                   <  2:3825349/100‑1 (MQ=255)
                aaTATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCa                                                                                  >  2:366510/1‑100 (MQ=255)
                aaTATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCa                                                                                  >  1:3422984/1‑100 (MQ=255)
                aaTATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCa                                                                                  >  2:2316330/1‑100 (MQ=255)
                  tatGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAAt                                                                                <  1:3345592/100‑1 (MQ=255)
                   atGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATg                                                                               <  2:1889152/100‑1 (MQ=255)
                    tGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGc                                                                              <  2:759129/100‑1 (MQ=255)
                      ggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAg                                                                            >  2:1465218/1‑100 (MQ=255)
                      ggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAg                                                                            >  2:2814950/1‑100 (MQ=255)
                      ggTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAg                                                                            <  1:1836502/100‑1 (MQ=255)
                       gTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGt                                                                           <  1:3183353/100‑1 (MQ=255)
                       gTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGt                                                                           <  2:2385071/100‑1 (MQ=255)
                       gTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGt                                                                           >  2:3275537/1‑100 (MQ=255)
                          gCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGa                                                                        >  2:2317698/1‑100 (MQ=255)
                           cccGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGAc                                                                       <  2:2152458/100‑1 (MQ=255)
                           cccGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGAc                                                                       <  2:2159241/100‑1 (MQ=255)
                           cccGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGAc                                                                       >  1:667975/1‑100 (MQ=255)
                           cccGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGAc                                                                       >  1:1338/1‑100 (MQ=255)
                            ccGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACg                                                                      <  1:193361/100‑1 (MQ=255)
                                cTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTcc                                                                  <  2:1382059/100‑1 (MQ=255)
                                cTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTcc                                                                  <  1:1007817/100‑1 (MQ=255)
                                cTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTcc                                                                  <  1:216196/100‑1 (MQ=255)
                                  aaaaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTg                                                                <  1:2563556/100‑1 (MQ=255)
                                  aaaaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTg                                                                <  2:127216/100‑1 (MQ=255)
                                  aaaaaaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTg                                                                >  2:1062813/1‑100 (MQ=255)
                                      aaGTGAATCGCAAGCTCGCAGTTTCAGCCCTCTGGCAGTACCCGGATGTCGTGAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTAc                                                            <  1:1288872/100‑1 (MQ=255)
                                      aaGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTAc                                                            <  2:3380428/100‑1 (MQ=255)
                                        gTGAATCGCAAGCCCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTAccc                                                          >  1:1267682/1‑100 (MQ=255)
                                           aaTCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTCCCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTg                                                        >  1:3965707/1‑99 (MQ=255)
                                           aaTCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGa                                                       >  2:3781426/1‑100 (MQ=255)
                                           aaTCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTg                                                        >  2:2038091/1‑99 (MQ=255)
                                            aTCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa                                                      <  2:485120/100‑1 (MQ=255)
                                            aTCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa                                                      <  1:271786/100‑1 (MQ=255)
                                               gCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGATCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGAt                                                   >  2:299839/1‑100 (MQ=255)
                                                cAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGAtt                                                  >  1:2895030/1‑100 (MQ=255)
                                                 aaGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTc                                                 <  2:3497166/100‑1 (MQ=255)
                                                  aGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCg                                                <  1:2137839/100‑1 (MQ=255)
                                                  aGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCg                                                <  1:626370/100‑1 (MQ=255)
                                                  aGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCg                                                <  1:1552820/100‑1 (MQ=255)
                                                    cTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCa                                              >  2:1314217/1‑100 (MQ=255)
                                                    cTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCa                                              >  2:1345991/1‑100 (MQ=255)
                                                    cTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCa                                              <  1:2857821/100‑1 (MQ=255)
                                                    cTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCa                                              <  2:2730129/100‑1 (MQ=255)
                                                    cTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCa                                              >  1:3538827/1‑100 (MQ=255)
                                                     tCGCAGGTTCAGCCCTCTTGCAGTACCCGGCTGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCAc                                             >  1:3393818/1‑100 (MQ=255)
                                                     tCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCAc                                             >  2:636318/1‑100 (MQ=255)
                                                     tCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGc                                               >  1:992257/1‑98 (MQ=255)
                                                     tCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCAc                                             <  2:2993168/100‑1 (MQ=255)
                                                     tCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCAc                                             <  1:2268543/100‑1 (MQ=255)
                                                      cGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCAcc                                            <  2:3569703/100‑1 (MQ=255)
                                                       gCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCg                                           <  2:3545404/100‑1 (MQ=255)
                                                          ggTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCt                                        <  1:1638277/100‑1 (MQ=255)
                                                            ttCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTgg                                      >  1:4195287/1‑100 (MQ=255)
                                                             tCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTggg                                     <  2:610115/100‑1 (MQ=255)
                                                             tCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTggg                                     <  2:4055657/100‑1 (MQ=255)
                                                             tCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTggg                                     <  1:3465146/100‑1 (MQ=255)
                                                              cAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCTGTCCTTACCGAGGTCGTCAAGGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGc                                    <  1:845252/100‑1 (MQ=255)
                                                               aGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCa                                   >  2:2453413/1‑100 (MQ=255)
                                                               aGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCCGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTgg                                      >  2:2640771/1‑97 (MQ=255)
                                                                gCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCAc                                  >  2:1214225/1‑100 (MQ=255)
                                                                 cccTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCAcc                                 <  1:559238/100‑1 (MQ=255)
                                                                 cccTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCAcc                                 <  1:2816909/100‑1 (MQ=255)
                                                                  ccTCTTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCg                                <  2:3210492/100‑1 (MQ=255)
                                                                   ctctTGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCgg                               <  1:946089/100‑1 (MQ=255)
                                                                   ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCgg                               <  1:2133033/100‑1 (MQ=255)
                                                                     ctTGCAGTACCCGGATGTCGTTAAATCAATTCTTACTGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCgg                               <  1:66416/98‑1 (MQ=255)
                                                                       tGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACTTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAac                            >  1:4017449/1‑99 (MQ=255)
                                                                       tGCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAaca                           >  2:1207202/1‑100 (MQ=255)
                                                                       tGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAaca                           <  2:3251711/100‑1 (MQ=255)
                                                                        gCAGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAacac                          >  2:4113535/1‑100 (MQ=255)
                                                                          aGTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCg                        >  2:61841/1‑100 (MQ=255)
                                                                           gTACCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGt                       >  2:595513/1‑100 (MQ=255)
                                                                           gTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGt                       <  1:2406124/100‑1 (MQ=255)
                                                                             aCCCGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTaa                     >  2:2405498/1‑100 (MQ=255)
                                                                              cccGGATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAAc                    >  1:1515235/1‑100 (MQ=255)
                                                                                 ggATGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGc                 <  1:3087231/100‑1 (MQ=255)
                                                                                    tGTCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGaa              <  1:3851203/100‑1 (MQ=255)
                                                                                    tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGaa              <  2:310049/100‑1 (MQ=255)
                                                                                      tCGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAaga            >  2:1186967/1‑100 (MQ=255)
                                                                                       cGTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag           <  2:1866035/100‑1 (MQ=255)
                                                                                       cGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag           >  1:1453375/1‑100 (MQ=255)
                                                                                        gTTAAATCGGTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAgaga          <  1:2077367/100‑1 (MQ=255)
                                                                                        gTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAgaga          <  1:3302190/100‑1 (MQ=255)
                                                                                             aTCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAAAGATTGc      >  1:3450566/1‑99 (MQ=255)
                                                                                               cGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCa   <  1:1703352/100‑1 (MQ=255)
                                                                                               cGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCa   <  1:1002648/100‑1 (MQ=255)
                                                                                                gATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa  >  2:1329203/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
CCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCGCAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAA  >  NC_000913/3410552‑3410747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: