Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,452,057 A→T 100% I77N (ATC→AAC)  rplD ← 50S ribosomal subunit protein L4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,452,0570AT97.4% 631.5 / 3.7 188I77N (ATC→AAC) rplD50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base T (102/81);  total (106/82)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

Only 100 of 188 total aligned reads displayed.

AGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAG  >  NC_000913/3451961‑3452153
                                                                                                |                                                                                                
 ggATGCTTTTCAGCGCGCCGCGGTACTTTTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTAACGCCACCAGAACGCCAGTTCgg                                                                                              >  1:3456441/1‑100 (MQ=255)
 ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                              >  2:3656618/1‑100 (MQ=255)
  gATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCggg                                                                                             >  1:1713643/1‑100 (MQ=255)
   aTGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGc                                                                                            <  1:1506510/100‑1 (MQ=255)
    tGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGct                                                                                           >  2:2826617/1‑100 (MQ=255)
    tGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGct                                                                                           >  2:4183065/1‑100 (MQ=255)
      cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                         >  2:3892520/1‑100 (MQ=255)
      cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                         >  1:2956959/1‑100 (MQ=255)
      cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                         >  2:3219118/1‑100 (MQ=255)
       ttttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCtt                                                                                        <  1:3754786/100‑1 (MQ=255)
       ttttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCAGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCtt                                                                                        <  1:2370733/100‑1 (MQ=255)
          tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                     <  1:3179433/100‑1 (MQ=255)
          tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                     >  1:4009056/1‑100 (MQ=255)
           cAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATa                                                                                    <  1:3286503/100‑1 (MQ=255)
           cAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATa                                                                                    <  1:723971/100‑1 (MQ=255)
             gcgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGa                                                                                  >  2:1576217/1‑100 (MQ=255)
             gcgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGa                                                                                  >  2:1508493/1‑100 (MQ=255)
                  ccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAg                                                                             <  2:174288/100‑1 (MQ=255)
                  ccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAg                                                                             >  2:1135748/1‑100 (MQ=255)
                  acgccGTACATCTTCTTGTTAACGTTTTGACTGTGCTCCTTCGCACGAACAGCAAAGCTCACGCCACCAGAACGCAAGTTCGGGCACTTCATAGAAc                                                                                >  1:1694906/2‑97 (MQ=255)
                    gcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGGCGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGaa                                                                           >  1:1454349/1‑100 (MQ=255)
                     cgGTACCTCTTCTTGTTAAATAATAAACTGTGGTCCTGCGGACAAGCAGCAAAGGTCACGCCACCAGAAAGCCAGTTCGGGCTGTTGATAGAACAAGAAc                                                                          >  1:3641629/1‑100 (MQ=255)
                      ggTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAcg                                                                         <  2:1873962/100‑1 (MQ=255)
                          cATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCa                                                                     <  2:2391106/100‑1 (MQ=255)
                          cATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCa                                                                     >  2:4077451/1‑100 (MQ=255)
                             cttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACgg                                                                  >  2:1639402/1‑100 (MQ=255)
                             cttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACgg                                                                  >  1:596882/1‑100 (MQ=255)
                              ttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGc                                                                 >  2:4048108/1‑100 (MQ=255)
                                  tGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGt                                                             >  2:3635237/1‑100 (MQ=255)
                                  tGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGt                                                             <  2:3389481/100‑1 (MQ=255)
                                   gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCGGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg                                                            >  2:2017808/1‑100 (MQ=255)
                                    ttAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGc                                                           <  2:1928111/100‑1 (MQ=255)
                                    ttAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGc                                                           >  2:1689915/1‑100 (MQ=255)
                                     tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                          >  2:185429/1‑100 (MQ=255)
                                     tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                          <  1:4023379/100‑1 (MQ=255)
                                     tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGAGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                          >  2:1100048/1‑100 (MQ=255)
                                        cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCttt                                                       >  2:2980456/1‑100 (MQ=255)
                                        cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCttt                                                       >  2:130700/1‑100 (MQ=255)
                                        cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCttt                                                       >  1:2789407/1‑100 (MQ=255)
                                        cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCttt                                                       >  2:3828948/1‑100 (MQ=255)
                                        cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCttt                                                       >  1:2930411/1‑100 (MQ=255)
                                            ttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTgg                                                   <  1:3412707/100‑1 (MQ=255)
                                             tGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgc                                                  >  2:3278971/1‑100 (MQ=255)
                                             tGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgc                                                  <  2:634386/100‑1 (MQ=255)
                                              gACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcg                                                 >  2:2678737/1‑100 (MQ=255)
                                               aCTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgc                                                  <  1:1570776/98‑1 (MQ=255)
                                                cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                               >  1:1253035/1‑100 (MQ=255)
                                                cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                               >  1:442006/1‑100 (MQ=255)
                                                cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                               <  2:1616426/100‑1 (MQ=255)
                                                cTGTGGTCCTGCGGACGAGCAGCAAAGATCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcgc                                                >  2:4080227/1‑99 (MQ=255)
                                                  gtgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCAc                                             >  2:2402598/1‑100 (MQ=255)
                                                    ggTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                           <  2:4070647/100‑1 (MQ=255)
                                                    ggTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                           >  2:3583769/1‑100 (MQ=255)
                                                     gTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                           >  1:2389155/1‑99 (MQ=255)
                                                     gTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGt                                          >  2:2792670/1‑100 (MQ=255)
                                                     gTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGt                                          >  2:156391/1‑100 (MQ=255)
                                                      tCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtt                                         >  2:294629/1‑100 (MQ=255)
                                                       ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttt                                        >  1:3137483/1‑100 (MQ=255)
                                                       ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttt                                        <  1:115206/100‑1 (MQ=255)
                                                       ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACCGCCGGTGCCTTTCTGGCGCCACgg                                           >  1:746761/1‑97 (MQ=255)
                                                        cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                       <  2:3968489/100‑1 (MQ=255)
                                                        cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                       <  2:918554/100‑1 (MQ=255)
                                                        cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                       <  1:3371257/100‑1 (MQ=255)
                                                        cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                       >  1:2201711/1‑100 (MQ=255)
                                                         tGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttttt                                      >  2:719090/1‑100 (MQ=255)
                                                           cGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTa                                    >  2:1057329/1‑100 (MQ=255)
                                                             gACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAcc                                  <  1:2293446/100‑1 (MQ=255)
                                                                 agcagcAAAGGTCGCGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCAGTGCCTTTCTGGCGCCACGGTTTTTTACCGGaa                              >  1:1017994/1‑100 (MQ=255)
                                                                  gcagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAc                             <  2:596884/100‑1 (MQ=255)
                                                                   cagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                            >  2:2495954/1‑100 (MQ=255)
                                                                   cagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCACACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                            <  1:926356/100‑1 (MQ=255)
                                                                    agcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCa                           >  2:2557093/1‑100 (MQ=255)
                                                                    agcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCa                           >  1:2828733/1‑100 (MQ=255)
                                                                     gcaAAGGTCACGCCACCAGAACGCCAGTTCGGACTCTTGATAGAACCAGAACGCGCACGGCTGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                          >  1:1402260/1‑100 (MQ=255)
                                                                       aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGtt                        <  2:1027561/100‑1 (MQ=255)
                                                                       aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGtt                        >  1:499832/1‑100 (MQ=255)
                                                                          ggTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACt                     >  1:196080/1‑100 (MQ=255)
                                                                          ggTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACt                     >  1:419989/1‑100 (MQ=255)
                                                                          ggTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCAACGGTGTTTTACAGGAACCAGTTACt                     >  1:3906122/1‑100 (MQ=255)
                                                                             cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa                  <  1:4054735/100‑1 (MQ=255)
                                                                             cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa                  <  1:719087/100‑1 (MQ=255)
                                                                             cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa                  >  2:216901/1‑100 (MQ=255)
                                                                              aCGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAg                 <  2:1267408/100‑1 (MQ=255)
                                                                              aCGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAg                 >  2:3912925/1‑100 (MQ=255)
                                                                               cGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGc                >  1:3243978/1‑100 (MQ=255)
                                                                               cGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCAACGGTTTTTTACCGGAACCAGTTACTTCAGc                >  1:3312451/1‑100 (MQ=255)
                                                                                 ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCAc              >  2:3019978/1‑100 (MQ=255)
                                                                                 ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCAc              <  2:542411/100‑1 (MQ=255)
                                                                                  caccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACg             >  1:1362304/1‑100 (MQ=255)
                                                                                    ccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg           >  1:2776220/1‑100 (MQ=255)
                                                                                    ccaGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg           <  1:4048088/100‑1 (MQ=255)
                                                                                      aGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtc         >  2:1701779/1‑100 (MQ=255)
                                                                                          cGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttct     <  2:1212378/100‑1 (MQ=255)
                                                                                           gCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg    <  2:2336567/100‑1 (MQ=255)
                                                                                           gCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg    <  2:2211297/100‑1 (MQ=255)
                                                                                           gCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg    <  1:868545/100‑1 (MQ=255)
                                                                                           gCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAACACGAttcttc      >  1:3311951/1‑98 (MQ=255)
                                                                                            ccAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGa   >  1:2226768/1‑100 (MQ=255)
                                                                                            ccAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGa   >  2:4238136/1‑100 (MQ=255)
                                                                                             cAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAg  <  2:2153913/100‑1 (MQ=255)
                                                                                                |                                                                                                
AGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAG  >  NC_000913/3451961‑3452153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: