Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,466,858 G→C 52.6% A45G (GCC→GGC)  bfd ← bacterioferritin‑associated ferredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,466,8580GC52.6% 24.2 / 246.5 156A45G (GCC→GGC) bfdbacterioferritin‑associated ferredoxin
Reads supporting (aligned to +/- strand):  ref base G (44/30);  new base C (51/31);  total (95/61)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 158 total aligned reads displayed.

AGAACGGCTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGCCTGACGAATTTTT  >  NC_000913/3466760‑3466956
                                                                                                  |                                                                                                  
  aaCGGCTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGcccc                                                                                                 >  1:3929685/1‑100 (MQ=255)
    cGGCTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCgg                                                                                               <  2:656818/100‑1 (MQ=255)
    cGGCTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCgg                                                                                               <  2:1927973/100‑1 (MQ=255)
      gCTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGac                                                                                             >  2:1294431/1‑100 (MQ=255)
      gCTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGac                                                                                             >  2:3873684/1‑100 (MQ=255)
      gCTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGac                                                                                             >  2:2184632/1‑100 (MQ=255)
      gCTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGac                                                                                             <  1:2669641/100‑1 (MQ=255)
       cTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTACTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGaca                                                                                            >  1:2330016/1‑100 (MQ=255)
         tACGAGTAAGTCAAAAAACACACTGCGTTTATGCGGGCTCCTCACACTCCGGCAGCTGCATTAATTCATCCTCCATCTCTTCACGCGCGCCCCGGAcaca                                                                                          <  1:3931065/100‑1 (MQ=255)
         tACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGAcaca                                                                                          >  2:1794424/1‑100 (MQ=255)
         tACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGAcaca                                                                                          <  2:1685501/100‑1 (MQ=255)
         tACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGAcaca                                                                                          >  2:257945/1‑100 (MQ=255)
              gtcaGTCAAAAAACACACTCCGTATATGCGGACTCCTTAAACTCCGCCACCTGCATAACTCCATCCTCCATCACTTCCCGCGCGGCCCGGACACCttt                                                                                       >  1:2592488/4‑98 (MQ=255)
              gTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTa                                                                                      >  1:828313/1‑99 (MQ=255)
               tAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTAcc                                                                                    <  2:3375596/100‑1 (MQ=255)
                  gTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCa                                                                                 >  1:1873989/1‑100 (MQ=255)
                  gTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCa                                                                                 >  1:2254739/1‑100 (MQ=255)
                  gTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCa                                                                                 >  1:1735433/1‑100 (MQ=255)
                   tCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCAc                                                                                >  1:2770194/1‑100 (MQ=255)
                   tCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCAc                                                                                >  1:171398/1‑100 (MQ=255)
                   tCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCAc                                                                                >  1:3182305/1‑100 (MQ=255)
                    cAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACt                                                                               >  1:2757872/1‑100 (MQ=255)
                     aaaaaaCACACTCCGTTTATGCGGACTCCTTAACCTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTg                                                                              <  2:1362762/100‑1 (MQ=255)
                     aaaaaaCACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTg                                                                              >  2:2849502/1‑100 (MQ=255)
                      aaaaaCACACTCCGTTTATGCGGACTCCTTAAACTCAGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGa                                                                             >  1:951766/1‑100 (MQ=255)
                           cacacTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGcc                                                                        <  1:1918436/100‑1 (MQ=255)
                              acTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGAc                                                                     <  1:3238952/100‑1 (MQ=255)
                               cTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGAcc                                                                    >  1:2851749/1‑100 (MQ=255)
                               cTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGAc                                                                     >  1:1327673/1‑99 (MQ=255)
                                 ccGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCgg                                                                  >  2:1405010/1‑100 (MQ=255)
                                      tATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATaa                                                             >  2:1643294/1‑100 (MQ=255)
                                      tATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATaa                                                             >  2:2709903/1‑100 (MQ=255)
                                        tGCGGCCTCCTTAAACTCCCGCAGCTGCATTAATTCATCCTCCCTCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGACGACAGGaaagtt                                                            >  1:1248639/1‑95 (MQ=255)
                                        tGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACCCATTTCCCGCACTGACTGCCGACCGGaacca                                                             >  1:3752443/1‑95 (MQ=255)
                                           ggACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAAtttt                                                        >  2:582045/1‑100 (MQ=255)
                                           ggACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAAtttt                                                        <  2:1758958/100‑1 (MQ=255)
                                            gACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAAttttt                                                       <  2:1693044/100‑1 (MQ=255)
                                             aCTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAAtttttt                                                      <  2:519539/100‑1 (MQ=255)
                                              cTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAAttttttt                                                     >  1:2923296/1‑100 (MQ=255)
                                               tCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTa                                                    <  1:1387608/100‑1 (MQ=255)
                                               tCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGAAACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTa                                                    <  1:2927341/100‑1 (MQ=255)
                                               tCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTa                                                    <  1:25941/100‑1 (MQ=255)
                                                ccTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTaa                                                   >  2:3538204/1‑100 (MQ=255)
                                                ccTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTaa                                                   >  2:363189/1‑100 (MQ=255)
                                                ccTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTaa                                                   >  2:138064/1‑100 (MQ=255)
                                                ccTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTaa                                                   >  1:4177470/1‑100 (MQ=255)
                                                 cTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAAt                                                  >  1:4197858/1‑100 (MQ=255)
                                                 cTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAAt                                                  >  1:3295057/1‑100 (MQ=255)
                                                 cTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTaag                                                  >  1:1673209/1‑99 (MQ=255)
                                                 cTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAAt                                                  >  1:773929/1‑100 (MQ=255)
                                                 cTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAAt                                                  >  1:148131/1‑100 (MQ=255)
                                                  ttAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTTAtt                                                 >  1:294680/1‑100 (MQ=255)
                                                  ttAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAAtt                                                 <  2:4118502/100‑1 (MQ=255)
                                                     gatctcGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCt                                              <  2:3891965/95‑1 (MQ=255)
                                                      aCTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTg                                             <  2:1826063/100‑1 (MQ=255)
                                                       cTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTgg                                            >  1:2496879/1‑100 (MQ=255)
                                                        tCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGa                                           <  1:2967012/100‑1 (MQ=255)
                                                          cGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCtagatc                                         >  1:3891947/1‑95 (MQ=255)
                                                           ggCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGaatg                                        >  2:21497/1‑98 (MQ=255)
                                                               gCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTg                                    >  1:2671634/1‑100 (MQ=255)
                                                                 tGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAg                                  >  2:2783847/1‑100 (MQ=255)
                                                                 tGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGCAATAAATTTTTTTAATTGCTGGAACGAGTGAg                                  >  1:3743573/1‑100 (MQ=255)
                                                                 tGCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAg                                  >  1:1355259/1‑100 (MQ=255)
                                                                  gCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAgg                                 >  2:1512706/1‑100 (MQ=255)
                                                                  gCATTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAgg                                 <  2:2036393/100‑1 (MQ=255)
                                                                   cATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAggg                                <  2:3777451/100‑1 (MQ=255)
                                                                   cATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAggg                                <  1:958419/100‑1 (MQ=255)
                                                                   cATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAggg                                <  2:2911594/100‑1 (MQ=255)
                                                                    aTTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAgggg                               <  2:3253638/100‑1 (MQ=255)
                                                                    aTTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAgggg                               >  1:1414921/1‑100 (MQ=255)
                                                                    aTTAATTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAgggg                               >  2:1444807/1‑100 (MQ=255)
                                                                       aaTTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGaaa                            >  1:830407/1‑100 (MQ=255)
                                                                       aaTTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTATTTGCTGGAATGAATCAGGGGaaa                            >  1:2167177/1‑100 (MQ=255)
                                                                       aaTTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGaaa                            >  2:3385976/1‑100 (MQ=255)
                                                                       aaTTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGaaa                            >  2:878666/1‑100 (MQ=255)
                                                                       aaTTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGaaa                            >  2:14431/1‑100 (MQ=255)
                                                                        aTTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGaaaa                           >  2:272006/1‑100 (MQ=255)
                                                                        aTTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGaaaa                           >  2:1568714/1‑100 (MQ=255)
                                                                        aTTCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGaaaa                           >  1:2004848/1‑100 (MQ=255)
                                                                         ttCATCCTCCATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAAc                          <  2:2923308/100‑1 (MQ=255)
                                                                             tcctccATCACTTCGCGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTAAGGGGAAAACTGgc                      <  1:724009/100‑1 (MQ=255)
                                                                             tcctccATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGgc                      <  2:2657086/100‑1 (MQ=255)
                                                                              cctccATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGgcg                     <  2:1603630/100‑1 (MQ=255)
                                                                              cctccATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGgcg                     <  2:3356173/100‑1 (MQ=255)
                                                                              cctccATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGgcg                     <  1:1566885/100‑1 (MQ=255)
                                                                               ctccATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGgcgc                    <  1:4095435/100‑1 (MQ=255)
                                                                               ctccATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGCGAGGGGAAAACTGgcgc                    >  1:148770/1‑100 (MQ=255)
                                                                                 ccATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGcac                  >  2:3605758/1‑100 (MQ=255)
                                                                                 ccATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGcac                  >  1:839569/1‑100 (MQ=255)
                                                                                  cATCACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGcaca                 >  2:3631969/1‑100 (MQ=255)
                                                                                   aTCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAg                >  2:434272/1‑100 (MQ=255)
                                                                                     cACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGcc              >  1:445623/1‑100 (MQ=255)
                                                                                     cACTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGcc              <  1:3091900/100‑1 (MQ=255)
                                                                                      aCTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGCCt             >  1:3369751/1‑100 (MQ=255)
                                                                                       cTTCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTTCTTGAACGAGTAAAGGCAACACTGCAGCACAGc               >  1:4072579/1‑97 (MQ=255)
                                                                                         tCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGCCTGAc          <  2:3926246/100‑1 (MQ=255)
                                                                                         tCACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGCCTGAc          <  2:1290364/100‑1 (MQ=255)
                                                                                          cACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGCCTGACg         >  1:1582241/1‑100 (MQ=255)
                                                                                          cACGCGCGCCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGCCTGAc          <  2:1503/99‑1 (MQ=255)
                                                                                                 ggCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGCCTGACGAAttttt  <  2:2496886/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
AGAACGGCTTACGAGTAAGTCAAAAAACACACTCCGTTTATGCGGACTCCTTAAACTCCGGCAGCTGCATTAATTCATCCTCCATCACTTCACGCGCGGCCCGGACACATTTACCGCACTGATTGCCGACCGGAATAAATTTTTTTAATTGCTGGAACGAGTGAGGGGAAAACTGGCGCACAGCCTGACGAATTTTT  >  NC_000913/3466760‑3466956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: