Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,357 C→T 16.3% intergenic (‑28/+43) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3570CT16.3% 502.9 / 70.0 207intergenic (‑28/+43)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base C (97/76);  new base T (20/14);  total (117/90)
Fisher's exact test for biased strand distribution p-value = 8.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

Only 100 of 217 total aligned reads displayed.

ACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATA  >  NC_000913/3471258‑3471456
                                                                                                   |                                                                                                   
 ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTccc                                                                                                    >  1:1078326/1‑100 (MQ=255)
 ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTccc                                                                                                    >  2:3122485/1‑100 (MQ=255)
 ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTccc                                                                                                    >  2:109173/1‑100 (MQ=255)
   gTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCt                                                                                                  >  2:2489913/1‑100 (MQ=255)
      gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCa                                                                                               >  2:4021414/1‑100 (MQ=255)
      gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTGTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTc                                                                                                <  1:2667663/99‑1 (MQ=255)
       tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                              <  2:3502799/100‑1 (MQ=255)
       tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                              >  1:1733081/1‑100 (MQ=255)
        cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                             >  2:2402501/1‑100 (MQ=255)
          aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                           <  1:1576050/100‑1 (MQ=255)
            gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                         >  1:3228801/1‑100 (MQ=255)
               gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                      <  1:702071/100‑1 (MQ=25)
                ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                      <  1:2040180/99‑1 (MQ=21)
                ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                                                     >  2:912214/1‑100 (MQ=255)
                ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                                                     >  2:1191102/1‑100 (MQ=255)
                 cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                    <  2:1451723/100‑1 (MQ=25)
                 cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCAc                                                                                    <  2:841194/100‑1 (MQ=255)
                 cGATAATACCAACGTTAACGTGCGGTTCTGTACGTTCAAATTTTTGTTTAGACACGGCTAGATTCCTTACTATAGTGCTCTCTCCTGAAGGGCAGAGCAc                                                                                    <  1:1983431/100‑1 (MQ=21)
                  gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACg                                                                                   >  2:3955883/1‑100 (MQ=255)
                   aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                  <  2:4191394/100‑1 (MQ=255)
                      gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGAc                                                                               >  1:3016718/1‑100 (MQ=255)
                        aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActatagta                                                                             <  1:641133/100‑8 (MQ=25)
                        aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                             >  1:133845/1‑100 (MQ=255)
                         ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTTCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                            >  1:977124/1‑100 (MQ=255)
                         ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                            >  2:2861469/1‑100 (MQ=255)
                         ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                            >  2:653704/1‑100 (MQ=255)
                          cAACGTTAACTTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTg                                                                           <  1:2800815/100‑1 (MQ=255)
                           aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                          >  2:3394773/1‑100 (MQ=255)
                            aCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                         <  2:736964/100‑1 (MQ=255)
                             cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                        <  1:2836210/100‑1 (MQ=255)
                                tAACGTGCGGTTTTGTCCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAt                                                                       <  1:3259311/98‑1 (MQ=255)
                                  aCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAc                                                                   <  2:1816751/100‑1 (MQ=255)
                                     tGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCt                                                                >  1:1750013/1‑100 (MQ=255)
                                       cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                              <  2:2887072/100‑1 (MQ=255)
                                       cGGTTTTCTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                              >  2:2234636/1‑100 (MQ=255)
                                        ggTTTTGTACGTTCAAATTTTTCTTCAGACACGGCTATATTCGTTCTTATAATGCTCTCCCCTTCAGGATAGAGCACGGGACTTTGGTATTAACCCtt                                                               >  1:1033232/1‑98 (MQ=255)
                                         gatttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCATACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGTTATTAACCCTTAg                                                             >  2:2317980/3‑99 (MQ=255)
                                           tttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCt                                                          <  2:1471602/100‑1 (MQ=255)
                                             tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                        <  1:2095322/100‑1 (MQ=255)
                                             tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                        >  2:479726/1‑100 (MQ=255)
                                             tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                        >  2:3287530/1‑100 (MQ=255)
                                              gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                                                       <  2:2349065/100‑1 (MQ=255)
                                                aCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTTCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAttt                                                     >  1:1767180/1‑100 (MQ=255)
                                                 cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTa                                                    <  2:4082566/100‑1 (MQ=255)
                                                  gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                   <  1:230036/100‑1 (MQ=255)
                                                  gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                   >  2:2675682/1‑100 (MQ=255)
                                                   ttCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAcc                                                  >  1:1130825/1‑100 (MQ=255)
                                                    tCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCa                                                 >  2:2735204/1‑100 (MQ=255)
                                                     cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                >  2:1551766/1‑100 (MQ=255)
                                                     cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                >  1:1236327/1‑100 (MQ=255)
                                                        aTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg                                             <  1:1659703/100‑1 (MQ=255)
                                                          ttttCTTTAGACACGGCTATATTCCTTTCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                           <  1:925619/100‑1 (MQ=255)
                                                          ttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                           <  1:2458833/100‑1 (MQ=255)
                                                             tCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa                                        <  2:2060532/100‑1 (MQ=255)
                                                              cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                       >  2:3661678/1‑100 (MQ=255)
                                                              cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                       <  1:23494/100‑1 (MQ=255)
                                                               tttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCAAGGGACTTTGGTATTAACACTTAGGCTTATTTACCACGGGCTTCAAt                                      >  2:1849882/1‑100 (MQ=255)
                                                                 tAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAtt                                     <  1:4027115/99‑1 (MQ=255)
                                                                   gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                  >  1:4142/1‑100 (MQ=255)
                                                                    acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                 <  1:448628/100‑1 (MQ=255)
                                                                     cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                 <  1:3738970/99‑1 (MQ=255)
                                                                     cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                >  2:605590/1‑100 (MQ=255)
                                                                       cGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCt                              >  2:1256970/1‑100 (MQ=255)
                                                                       cGGCTATATCCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                >  1:2624752/1‑98 (MQ=255)
                                                                        ggCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTg                             <  1:3524596/100‑1 (MQ=255)
                                                                         gCTATATTCCTTACTATAGTGCTCTCCCCTTCCGGAGAGAGCACGGGACTTTGGTATTACCCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                            >  1:3826255/1‑100 (MQ=255)
                                                                         gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                            <  1:3955864/100‑1 (MQ=255)
                                                                          cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                           <  1:2161885/100‑1 (MQ=255)
                                                                           tataTTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                          >  2:3243450/1‑100 (MQ=255)
                                                                             tatTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGAGCTTCAATTACGGCCTGAGCaa                        >  2:2085518/1‑100 (MQ=255)
                                                                               ttCCTTACTATAGTGCTCTCCCCTTCAGGATAGCGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGTGCTTCAATTACGGACTGAGCAACg                      >  1:2805168/1‑100 (MQ=255)
                                                                               ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACTCTTAGGCTTATTTACCACGGGCTTCAATTAGGGCCTGAGCAACg                      >  1:3362517/1‑100 (MQ=255)
                                                                                 ccTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGACCTGAGCAACGtt                    >  1:2296881/1‑100 (MQ=255)
                                                                                    tACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACt                 >  2:4218462/1‑100 (MQ=255)
                                                                                    tACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGACTGAGCAACGTTACt                 >  2:307328/1‑100 (MQ=255)
                                                                                     aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                >  1:2431295/1‑100 (MQ=255)
                                                                                      cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg               >  2:2531247/1‑100 (MQ=255)
                                                                                      cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGATTCAATTACGGCCTGAGCAACGTTCCt                 >  1:1954260/1‑98 (MQ=255)
                                                                                       tataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCgg              >  1:2344197/1‑100 (MQ=255)
                                                                                        cccgTGCTCTCTCCTGTAGGGGAGAGCACGAGACTTTGGTCTTAACCCTTCGGCTTATTTACCACGGGCTACAATTACGTCCTGAGCAACGTTACTCGgc             >  1:457989/4‑100 (MQ=25)
                                                                                        cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc             >  2:1323744/4‑100 (MQ=38)
                                                                                        ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc             >  2:3845825/1‑100 (MQ=255)
                                                                                        ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc             <  1:3473178/100‑1 (MQ=255)
                                                                                         taGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg            <  1:2922102/100‑1 (MQ=255)
                                                                                         ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg            >  2:3058187/3‑100 (MQ=38)
                                                                                         ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg            >  2:113073/3‑100 (MQ=38)
                                                                                         ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg            >  1:2786209/3‑100 (MQ=38)
                                                                                         ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg            >  2:1990273/3‑100 (MQ=38)
                                                                                          cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc           >  2:1866123/2‑100 (MQ=38)
                                                                                          cgTGCTCTCTCCTGAAGGGGAGAGCACGCGCCTTTGGTATTCAGACTTAGGCTTATTTACCACGGGCTATAATCACAGCTTGAGCGACGTTACTCgg              >  1:3653126/2‑97 (MQ=16)
                                                                                           gTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt          >  1:20671/1‑100 (MQ=38)
                                                                                           gTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt          >  2:148372/1‑100 (MQ=38)
                                                                                           gTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTGATTAACCACGTGCTTGTATTACGGCGTGAGCAACGTTACGCGtcg            >  1:1475170/1‑98 (MQ=21)
                                                                                           gTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt          >  2:281704/1‑100 (MQ=255)
                                                                                           gTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt          <  2:1587012/100‑1 (MQ=255)
                                                                                            tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCCCtt         >  1:683963/1‑100 (MQ=255)
                                                                                             gCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc        >  1:1192104/1‑100 (MQ=255)
                                                                                                ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc     >  2:1755126/1‑100 (MQ=255)
                                                                                                ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc     <  1:2900115/100‑1 (MQ=255)
                                                                                                  ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTcatcat   >  2:3000540/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATA  >  NC_000913/3471258‑3471456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: