Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,361 T→G 15.9% intergenic (‑32/+39) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3610TG15.9% 526.6 / 90.4 212intergenic (‑32/+39)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base T (102/76);  new base G (20/14);  total (122/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.12e-01

Only 100 of 215 total aligned reads displayed.

GGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTC  >  NC_000913/3471263‑3471460
                                                                                                  |                                                                                                   
 gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTGTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTc                                                                                                    <  1:2667663/99‑1 (MQ=255)
 gTCAACGTGGCCGATAGTACCAACGTTAACGGGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAATGCTCTCCCATTCa                                                                                                   >  1:2451378/1‑100 (MQ=255)
  tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                                  <  2:3502799/100‑1 (MQ=255)
   cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                                 >  1:118590/1‑100 (MQ=255)
     aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGCGCTCTCCCCTTCAGgag                                                                                               <  1:2856743/100‑1 (MQ=255)
        tGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagaga                                                                                            >  2:3180388/1‑100 (MQ=255)
          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                          <  2:608172/100‑1 (MQ=21)
          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                          <  1:702071/100‑1 (MQ=25)
          gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                          >  2:2132547/1‑100 (MQ=255)
           ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                                                         >  2:1191102/1‑100 (MQ=255)
           ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                                                         >  2:912214/1‑100 (MQ=255)
            cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                        <  1:2130335/100‑1 (MQ=25)
            cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCAc                                                                                        <  2:841194/100‑1 (MQ=255)
            cGATAATACCAACGTTAACGTGCGGTTCTGTACGTTCAAATTTTTGTTTAGACACGGCTAGATTCCTTACTATAGTGCTCTCTCCTGAAGGGCAGAGCAc                                                                                        <  1:1983431/100‑1 (MQ=21)
               tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTATACTCGGCTATATTCCTTACTATAGTGCTATCCCCTTCAGGAGAGAGCACggg                                                                                     >  1:1305003/1‑100 (MQ=255)
               tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActat                                                                                     <  2:3866455/100‑4 (MQ=25)
                   aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCActatagta                                                                                 <  1:641133/100‑8 (MQ=25)
                    ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTTCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                >  1:977124/1‑100 (MQ=255)
                    ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                >  2:653704/1‑100 (MQ=255)
                    ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                >  2:2861469/1‑100 (MQ=255)
                      aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                              <  1:1445612/100‑1 (MQ=255)
                      aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                              >  2:3394773/1‑100 (MQ=255)
                       aCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGt                                                                             <  2:736964/100‑1 (MQ=255)
                         gTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAt                                                                           <  1:2185632/100‑1 (MQ=255)
                          ttAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                          <  2:1636418/100‑1 (MQ=255)
                           tAACGTGCGGTTTTGTCCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAt                                                                           <  1:3259311/98‑1 (MQ=255)
                             aCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAc                                                                       <  1:1753915/100‑1 (MQ=255)
                               gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAccc                                                                     >  2:1101957/1‑100 (MQ=255)
                                 gCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                                   >  2:1850444/1‑100 (MQ=255)
                                  cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                                  <  2:2887072/100‑1 (MQ=255)
                                   ggTTTTGTACGTTCAAATTTTTCTTCAGACACGGCTATATTCGTTCTTATAATGCTCTCCCCTTCAGGATAGAGCACGGGACTTTGGTATTAACCCtt                                                                   >  1:1033232/1‑98 (MQ=255)
                                    gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                >  2:2379857/1‑100 (MQ=255)
                                     ttttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGTGACTTTGGTATTAACCCTTAGGc                                                               <  2:1495093/100‑1 (MQ=255)
                                       ttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCt                                                              <  2:1842427/99‑1 (MQ=255)
                                        tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                            <  2:2441775/100‑1 (MQ=255)
                                        tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                            >  2:3287530/1‑100 (MQ=255)
                                         gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                                                           <  2:2349065/100‑1 (MQ=255)
                                         gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                                                           >  1:3963857/1‑100 (MQ=255)
                                            cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTa                                                        >  1:1701816/1‑100 (MQ=255)
                                             gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                       >  2:2675682/1‑100 (MQ=255)
                                             gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                       <  1:230036/100‑1 (MQ=255)
                                               tCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCa                                                     >  1:3953890/1‑100 (MQ=255)
                                                cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                    >  2:1551766/1‑100 (MQ=255)
                                                cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                    >  2:4196616/1‑100 (MQ=255)
                                                cAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAAAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                    >  1:1229722/1‑100 (MQ=255)
                                                   aTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg                                                 <  2:3952371/100‑1 (MQ=255)
                                                    tttttCTTTAGACACGGCTATATTCCTTACTATATTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                <  1:691210/100‑1 (MQ=255)
                                                    tttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                <  2:3094033/100‑1 (MQ=255)
                                                     ttttCTTTAGACACGGCTATATTCCTTTCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                               <  1:925619/100‑1 (MQ=255)
                                                     ttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                               <  1:2401492/100‑1 (MQ=255)
                                                        tCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa                                            <  2:2060532/100‑1 (MQ=255)
                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                           <  1:2041999/100‑1 (MQ=255)
                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                           >  2:3661678/1‑100 (MQ=255)
                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                           >  2:1389844/1‑100 (MQ=255)
                                                         cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                           >  2:1927971/1‑100 (MQ=255)
                                                          tttAGACACGGCCATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTCTTTACAACGGGCTTCAAt                                          >  2:3997271/1‑100 (MQ=255)
                                                            tAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAtt                                         <  1:4027115/99‑1 (MQ=255)
                                                             aGACACGGCTATATTCCTTACTCTAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTAc                                       <  1:1127093/100‑1 (MQ=255)
                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                      >  1:4142/1‑100 (MQ=255)
                                                              gACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACg                                      >  1:282058/1‑100 (MQ=255)
                                                               acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                     <  1:448628/100‑1 (MQ=255)
                                                                cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                     <  1:3738970/99‑1 (MQ=255)
                                                                cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                    <  2:2802949/100‑1 (MQ=255)
                                                                  cGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCt                                  >  2:147290/1‑100 (MQ=255)
                                                                  cGGCTATATCCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                    >  1:2624752/1‑98 (MQ=255)
                                                                   ggCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTg                                 <  2:1781636/100‑1 (MQ=255)
                                                                   ggCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTg                                 <  1:3524596/100‑1 (MQ=255)
                                                                     cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                               <  2:1298380/100‑1 (MQ=255)
                                                                     cTATATTCCTGACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                               <  2:4141303/100‑1 (MQ=255)
                                                                      tataTTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACTGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                              >  1:1749938/1‑100 (MQ=255)
                                                                      tataTTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                              >  2:3243450/1‑100 (MQ=255)
                                                                         atTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAAc                           >  2:114127/1‑100 (MQ=255)
                                                                          ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACTCTTAGGCTTATTTACCACGGGCTTCAATTAGGGCCTGAGCAACg                          >  1:3362517/1‑100 (MQ=255)
                                                                          ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGTGCTTCAATTACGGCCTGAGCAACg                          <  1:1647362/100‑1 (MQ=255)
                                                                            ccTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGtt                        >  2:4041004/1‑100 (MQ=255)
                                                                            ccTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGACCTGAGCAACGtt                        >  1:2296881/1‑100 (MQ=255)
                                                                             cTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTa                       >  1:2804806/1‑100 (MQ=255)
                                                                               tACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACt                     >  2:598272/1‑100 (MQ=255)
                                                                                 cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                   >  2:2531247/1‑100 (MQ=255)
                                                                                 cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                   >  2:2819578/1‑100 (MQ=255)
                                                                                 cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGATTCAATTACGGCCTGAGCAACGTTCCt                     >  1:1954260/1‑98 (MQ=255)
                                                                                  tataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCgg                  >  1:2344245/1‑100 (MQ=255)
                                                                                   cccgTGCTCTCTCCTGTAGGGGAGAGCACGAGACTTTGGTCTTAACCCTTCGGCTTATTTACCACGGGCTACAATTACGTCCTGAGCAACGTTACTCGgc                 >  1:457989/4‑100 (MQ=25)
                                                                                   cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                 >  2:1462039/4‑100 (MQ=38)
                                                                                   ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                 <  1:3473178/100‑1 (MQ=255)
                                                                                    taGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                <  1:2922102/100‑1 (MQ=255)
                                                                                    ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                >  1:2786209/3‑100 (MQ=38)
                                                                                    ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                >  2:1990273/3‑100 (MQ=38)
                                                                                    ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                >  2:3058187/3‑100 (MQ=38)
                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc               >  2:2271762/2‑100 (MQ=38)
                                                                                     cgTGCTCTCTCCTGAAGGGGAGAGCACGCGCCTTTGGTATTCAGACTTAGGCTTATTTACCACGGGCTATAATCACAGCTTGAGCGACGTTACTCgg                  >  1:3653126/2‑97 (MQ=16)
                                                                                      gTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTGATTAACCACGTGCTTGTATTACGGCGTGAGCAACGTTACGCGtcg                >  1:1475170/1‑98 (MQ=21)
                                                                                       tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTCTTTACCACGGGCTTCAATTACGCCCTGAGCAACGTTACTCGgcg                >  1:2518331/1‑97 (MQ=38)
                                                                                       tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt             >  1:1781321/1‑100 (MQ=255)
                                                                                        gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc            <  2:422099/100‑1 (MQ=255)
                                                                                           ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc         <  1:2900115/100‑1 (MQ=255)
                                                                                             ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTcatcat       >  1:3090525/1‑100 (MQ=255)
                                                                                             ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTcatcat       >  2:3000540/1‑100 (MQ=255)
                                                                                              cccTTCAGGAGAGAGCACGGAACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATa      >  1:2082103/1‑100 (MQ=255)
                                                                                                  tCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTc  <  1:300963/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTC  >  NC_000913/3471263‑3471460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: