Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 488,815 G→A 44.0% A760A (GCG→GCA mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913488,8150GA44.0% 84.2 / 211.2 175A760A (GCG→GCAmscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base G (41/57);  new base A (45/32);  total (86/89)
Fisher's exact test for biased strand distribution p-value = 3.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 182 total aligned reads displayed.

AACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTA  >  NC_000913/488716‑488912
                                                                                                   |                                                                                                 
aaCCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGgcg                                                                                                   <  2:3055341/100‑1 (MQ=255)
aaCCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGgcg                                                                                                   <  2:2508831/100‑1 (MQ=255)
  ccGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGcgcg                                                                                                 <  1:4102389/100‑1 (MQ=255)
  ccGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGcgcg                                                                                                 >  2:1571761/1‑100 (MQ=255)
   cGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGcacg                                                                                                 >  2:3793239/1‑99 (MQ=255)
   cGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGt                                                                                                >  2:2599404/1‑100 (MQ=255)
    gTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTc                                                                                               <  1:3608697/100‑1 (MQ=255)
     tttATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTc                                                                                               <  1:3980659/99‑1 (MQ=255)
       tATCTGGTGATCATCTGGAACCTGCTGTACCAGAAGGTACTGCGTTGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGGGTCGTGCGCTGGCGCGTCgg                                                                                             >  1:2836152/1‑99 (MQ=255)
        aTCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCa                                                                                           <  2:1837024/100‑1 (MQ=255)
         tCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAg                                                                                          >  2:2860954/1‑100 (MQ=255)
         tCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAg                                                                                          <  1:2395188/100‑1 (MQ=255)
          cTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAg                                                                                          <  1:2253329/99‑1 (MQ=255)
          cTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAg                                                                                          >  2:560576/1‑99 (MQ=255)
           tGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGaa                                                                                        >  2:1873711/1‑100 (MQ=255)
            ggTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAAt                                                                                       >  1:2603881/1‑100 (MQ=255)
                atcatcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTgg                                                                                   >  2:4071055/1‑100 (MQ=255)
                atcatcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTgg                                                                                   >  2:3580630/1‑100 (MQ=255)
                atcatcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTgg                                                                                   <  2:4129835/100‑1 (MQ=255)
                atcatcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTgg                                                                                   <  1:4168806/100‑1 (MQ=255)
                 tgctcTGCAACCTGCTGTACCAGACGGTGCTTGGTGGCTTAAGTGTAGAGGCGCGGCGTATCGCCTGTCGTCGTGCGCTGGCGCGTCGGCAGAATCTgg                                                                                   <  1:810603/96‑1 (MQ=255)
                 tcatcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGt                                                                                  <  1:3818260/100‑1 (MQ=255)
                   atcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGa                                                                                <  2:3104283/100‑1 (MQ=255)
                      tGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAag                                                                             <  1:1523211/100‑1 (MQ=255)
                       ggAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAaga                                                                            >  1:3289472/1‑100 (MQ=255)
                       ggAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAaga                                                                            <  2:3158991/100‑1 (MQ=255)
                       ggAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAag                                                                             >  2:908620/1‑99 (MQ=255)
                        gAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAagag                                                                           <  2:3123877/100‑1 (MQ=255)
                        gAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAagag                                                                           >  1:1742095/1‑100 (MQ=255)
                          aCCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAggg                                                                         <  1:2860943/100‑1 (MQ=255)
                            ctgctgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGgcg                                                                       <  1:690874/100‑1 (MQ=255)
                            ctgctgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGgcg                                                                       >  2:1797174/1‑100 (MQ=255)
                             tgctgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGgcgc                                                                      <  2:3906318/100‑1 (MQ=255)
                               ctgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAg                                                                    >  2:1179763/1‑100 (MQ=255)
                                tgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGa                                                                   <  2:2529060/100‑1 (MQ=255)
                                 gTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGa                                                                   <  1:3859732/99‑1 (MQ=255)
                                  tACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAg                                                                 >  1:3263095/1‑100 (MQ=255)
                                   aCCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAgg                                                                >  1:4001864/1‑100 (MQ=255)
                                   aCCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCCGGTGAAAGAGGGCGCAGAAgg                                                                >  1:1601943/1‑100 (MQ=255)
                                    ccAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGt                                                               <  1:484290/100‑1 (MQ=255)
                                    ccAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGt                                                               >  2:1480481/1‑100 (MQ=255)
                                     cAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTg                                                              <  1:3125184/100‑1 (MQ=255)
                                      aGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGc                                                             <  1:1830304/100‑1 (MQ=255)
                                      aGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGGGAAAGAGGGCGCAGACGGTGc                                                             >  2:3198881/1‑100 (MQ=255)
                                        aCGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTg                                                           >  1:3492087/1‑100 (MQ=255)
                                        aCGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCt                                                            <  1:2457321/99‑1 (MQ=255)
                                          ggTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGaa                                                         <  1:1073191/100‑1 (MQ=255)
                                          ggTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGaa                                                         >  2:3329801/1‑100 (MQ=255)
                                          ggTACAGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGaa                                                         <  2:2174344/100‑1 (MQ=255)
                                               tGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAccgcc                                                    >  1:1423678/1‑100 (MQ=255)
                                               tGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAccgcc                                                    <  2:3492100/100‑1 (MQ=255)
                                               tGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAccgcc                                                    >  2:3710535/1‑100 (MQ=255)
                                                gCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAccgccg                                                   >  2:125454/1‑100 (MQ=255)
                                                gCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAccgccg                                                   >  1:2176239/1‑100 (MQ=255)
                                                 cGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCgg                                                  <  1:3699820/100‑1 (MQ=255)
                                                 cGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCgg                                                  >  1:558677/1‑100 (MQ=255)
                                                  gTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGga                                                 <  1:740223/100‑1 (MQ=255)
                                                  gTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGga                                                 <  1:1705959/100‑1 (MQ=255)
                                                  gTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGga                                                 <  1:3474592/100‑1 (MQ=255)
                                                  gTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGCATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCgg                                                  >  1:2971788/1‑99 (MQ=255)
                                                   tGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGgaa                                                >  2:1147141/1‑100 (MQ=255)
                                                    ggCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGgaag                                               >  1:1395677/1‑100 (MQ=255)
                                                      cTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGgaagaa                                             >  2:1420788/1‑100 (MQ=255)
                                                       ttAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAAc                                            >  2:2361569/1‑100 (MQ=255)
                                                        tAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAAc                                            <  1:1797401/99‑1 (MQ=255)
                                                         aaGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAAccc                                          <  1:769132/100‑1 (MQ=255)
                                                          aGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACcca                                         >  2:1612937/1‑100 (MQ=255)
                                                           ncGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACccac                                        >  2:2208628/2‑100 (MQ=255)
                                                                gcggcgcggcGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTg                                   <  1:1220329/100‑1 (MQ=255)
                                                                 cggcgcggcgTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGGAGACGTGGCGGGACCGACTGAAGAACCCACCAtttc                                  >  1:2742116/1‑98 (MQ=255)
                                                                  ggcgcggcgTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCa                                 >  1:2032722/1‑100 (MQ=255)
                                                                  ggcgcggcgTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCa                                 >  2:993994/1‑100 (MQ=255)
                                                                   gcgcggcgTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCAc                                <  1:44784/100‑1 (MQ=255)
                                                                   gcgcggcgTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCAc                                <  2:498745/100‑1 (MQ=255)
                                                                    cgcggcgTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACt                               <  1:4250861/100‑1 (MQ=255)
                                                                      cggcgTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTgg                             <  2:956066/100‑1 (MQ=255)
                                                                        gcgTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAg                           <  2:1015160/100‑1 (MQ=255)
                                                                        gcgTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAg                           <  2:4232690/100‑1 (MQ=255)
                                                                        gcgTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGCAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAg                           <  1:2264464/100‑1 (MQ=255)
                                                                            aTCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAg                       >  2:1006311/1‑100 (MQ=255)
                                                                             tCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGt                      <  2:4000561/100‑1 (MQ=255)
                                                                               gCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTa                    >  1:4246834/1‑100 (MQ=255)
                                                                               gCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTa                    >  2:1492389/1‑100 (MQ=255)
                                                                                 cTGGGGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAc                  <  1:1249965/100‑1 (MQ=255)
                                                                                 cTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAc                  >  2:630960/1‑100 (MQ=255)
                                                                                  tGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAcc                 <  1:3151507/100‑1 (MQ=255)
                                                                                  tGGCGTCGGGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAcc                 <  1:2692476/100‑1 (MQ=255)
                                                                                   ggCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACca                >  1:2522430/1‑100 (MQ=255)
                                                                                     cgtcgtGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACcagc              <  1:547094/100‑1 (MQ=255)
                                                                                     cgtcgtGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACcagc              <  2:2542766/100‑1 (MQ=255)
                                                                                     cgtcgtGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACcagc              >  1:2885210/1‑100 (MQ=255)
                                                                                      gtcgtGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACcagca             <  1:3735866/100‑1 (MQ=255)
                                                                                          tGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACg         >  2:667102/1‑100 (MQ=255)
                                                                                           gcgcTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGc        >  1:1501943/1‑100 (MQ=255)
                                                                                             gcTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACg         >  2:3000191/1‑97 (MQ=255)
                                                                                              cTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGc     >  1:1330339/1‑100 (MQ=255)
                                                                                              cTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGc     >  1:889990/1‑100 (MQ=255)
                                                                                              cTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGc     >  1:2652976/1‑100 (MQ=255)
                                                                                              cTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGc     <  2:3475212/100‑1 (MQ=255)
                                                                                                ggggcgTCGGCAGAATCTTGTGAGAGAGGGCGCTGAAGGTGCTTAACCGCCCAAAGAACCCGCTATTGCACTGGTGCAAGTTTACCAGCAGACGCTGCg    <  1:1936401/96‑1 (MQ=255)
                                                                                                   |                                                                                                 
AACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTA  >  NC_000913/488716‑488912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: