BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,636,979	A→T	47.8%	D144E (GAT→GAA)	yhiN ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,979	0	A	T	47.8%	24.6 / 266.2	187	D144E (GAT→GAA)	yhiN	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (44/51); new base T (39/49); total (87/100)
Fisher's exact test for biased strand distribution p-value = 8.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 191 total aligned reads displayed.

GGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGCATGTC  >  NC_000913/3636880‑3637077

 gCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGAt                                                                                                   >  2:2174612/1‑100 (MQ=255)

   aaTCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCgttcagttca                                                                                                 <  2:4141864/100‑1 (MQ=255)

   aaTCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCa                                                                                                 <  1:1520407/100‑1 (MQ=255)

    aTCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCaa                                                                                                <  1:1224068/100‑1 (MQ=255)

      cTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGc                                                                                              >  1:2931233/1‑100 (MQ=255)

      cTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGc                                                                                              >  1:1305170/1‑100 (MQ=255)

          taACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGa                                                                                          >  1:2528696/1‑100 (MQ=255)

            aCCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAg                                                                                        >  1:1657228/1‑100 (MQ=255)

            aCCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAg                                                                                        >  2:1303257/1‑100 (MQ=255)

            aCCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAg                                                                                        >  2:2828581/1‑100 (MQ=255)

            aCCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAg                                                                                        <  2:90984/100‑1 (MQ=255)

            aCCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAg                                                                                        >  2:449119/1‑100 (MQ=255)

             ccAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGc                                                                                       <  1:1163778/100‑1 (MQ=255)

              taaaCGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGcc                                                                                      <  1:3539190/99‑1 (MQ=255)

              cAAACGGCGACGCGCCCAGCCCCGGCATTGACTGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGcc                                                                                      >  2:4052174/1‑100 (MQ=255)

              cAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGcc                                                                                      >  2:102182/1‑100 (MQ=255)

              cAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGcc                                                                                      >  1:1766100/1‑100 (MQ=255)

               aaaCGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGCTGACCAGCTTTTCGCAACCGACAGTAATGCCGTTCAGATCAAGCGTGAAGCCt                                                                                     >  1:113124/1‑100 (MQ=255)

               aaaCGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGcca                                                                                     >  2:3539203/1‑99 (MQ=255)

                       aCGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATc                                                                             <  2:1758653/100‑1 (MQ=255)

                       aCGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATc                                                                             <  1:1368862/100‑1 (MQ=255)

                       aCGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCCCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCAt                                                                              <  2:4061027/99‑1 (MQ=255)

                        cgcgCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATcc                                                                            <  1:3134580/100‑1 (MQ=255)

                        cgcgCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATcc                                                                            <  2:1407172/100‑1 (MQ=255)

                         gcgcCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACATTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATc                                                                             <  1:3680817/98‑1 (MQ=255)

                         gcgcCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCt                                                                           <  2:3704206/100‑1 (MQ=255)

                           gcCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTc                                                                         >  2:577316/1‑100 (MQ=255)

                            cccAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTc                                                                         <  1:965589/99‑1 (MQ=255)

                            cccAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCg                                                                        <  2:3794486/100‑1 (MQ=255)

                             ccAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGc                                                                       <  1:3825341/100‑1 (MQ=255)

                              cAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGcc                                                                      <  1:3954199/100‑1 (MQ=255)

                              cAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTGCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGcc                                                                      <  1:2784239/100‑1 (MQ=255)

                              cAGCCCCGGCAGTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCAGGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGcc                                                                      >  1:4005870/1‑100 (MQ=255)

                               aGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGccn                                                                     <  1:665410/100‑2 (MQ=255)

                               aGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCca                                                                     <  1:711526/100‑1 (MQ=255)

                               aGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGcc                                                                      <  1:4168292/99‑1 (MQ=255)

                                 ccccGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCcaca                                                                   >  1:1554362/1‑100 (MQ=255)

                                   ccGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACt                                                                 <  2:3316105/100‑1 (MQ=255)

                                       cATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGc                                                             <  1:375446/100‑1 (MQ=255)

                                        aTTGACAGCCCACCAGTCGCGGTGACCAGCTTTTGGCAACCGACAGTCATGCCGGTCAGTTCAAGCGTGAAGCCCGTTTCATCCTTCGccgcgttcagta                                                            >  1:2760977/1‑90 (MQ=255)

                                        aTTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCa                                                            >  2:3201682/1‑100 (MQ=255)

                                         ttGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCTACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCAc                                                           <  2:2542630/100‑1 (MQ=255)

                                         ttGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCAc                                                           <  2:490150/100‑1 (MQ=255)

                                         ttGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCAc                                                           <  2:368336/100‑1 (MQ=255)

                                           gACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACtt                                                         >  1:717205/1‑100 (MQ=255)

                                           gACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACtt                                                         >  2:1525053/1‑100 (MQ=255)

                                           gACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACtt                                                         >  1:1047189/1‑100 (MQ=255)

                                              aGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGc                                                      <  2:2945204/100‑1 (MQ=255)

                                               gCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCt                                                     <  2:3028389/100‑1 (MQ=255)

                                               gCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCt                                                     >  2:1627615/1‑100 (MQ=255)

                                                cccACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCCGCACTTCGCTa                                                    >  1:326930/1‑100 (MQ=255)

                                                 ccaccaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTAc                                                   <  1:3855762/100‑1 (MQ=255)

                                                  caccaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACg                                                  >  2:4235881/1‑100 (MQ=255)

                                                  caccaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACg                                                  <  2:3395829/100‑1 (MQ=255)

                                                   accaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGc                                                 >  1:919815/1‑100 (MQ=255)

                                                   accaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGc                                                 <  1:577314/100‑1 (MQ=255)

                                                    ccaGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCa                                                <  1:4243480/100‑1 (MQ=255)

                                                       gTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATc                                             >  2:2419608/1‑100 (MQ=255)

                                                       gTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATc                                             <  2:71160/100‑1 (MQ=255)

                                                       gTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCGGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATc                                             >  1:1418677/1‑100 (MQ=255)

                                                         cgcgATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTg                                           >  2:1892766/1‑100 (MQ=255)

                                                            gATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGGAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAg                                        <  1:1894784/100‑1 (MQ=255)

                                                             aTGATCAGCTTTTCGCAACCGACAGTCATTCCTTTCAGATCAGGCGTCAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAgg                                       <  1:2160642/100‑1 (MQ=255)

                                                             aTGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAgg                                       <  2:267975/100‑1 (MQ=255)

                                                             aTGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAg                                        <  1:789603/99‑1 (MQ=255)

                                                             aTGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAgg                                       <  1:1833022/100‑1 (MQ=255)

                                                               gACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTc                                     <  1:62486/100‑1 (MQ=255)

                                                                nccAGCTTTTCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTc                                     >  1:2367944/2‑99 (MQ=255)

                                                                 ccAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTcac                                   >  2:558638/1‑100 (MQ=255)

                                                                 ccAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTcac                                   >  2:517057/1‑100 (MQ=255)

                                                                 ccAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTcac                                   >  1:3434075/1‑100 (MQ=255)

                                                                  cAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTcaca                                  >  2:1953493/1‑100 (MQ=255)

                                                                    gCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACAtt                                >  1:3783954/1‑100 (MQ=255)

                                                                      ttttCGCAACCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGc                              <  1:190504/100‑1 (MQ=255)

                                                                      ttttCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTTATCCTGAGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGc                              <  1:1003523/100‑1 (MQ=255)

                                                                      ttttCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGc                              <  2:1049270/100‑1 (MQ=255)

                                                                         tCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGGcaatgtgacct                           <  2:3673777/100‑10 (MQ=255)

                                                                          cGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCtt                          >  2:2241333/1‑100 (MQ=255)

                                                                          cGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCtt                          >  2:2161693/1‑100 (MQ=255)

                                                                             aaCCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTtctc                       <  2:1865136/100‑1 (MQ=255)

                                                                              aCCGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCg                      <  2:426983/100‑1 (MQ=255)

                                                                               ccGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGc                     <  2:1657233/100‑1 (MQ=255)

                                                                               ccGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCACCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGc                     >  1:2572884/1‑100 (MQ=255)

                                                                                cGACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTtctc                       >  1:1320112/1‑97 (MQ=255)

                                                                                cGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCa                    >  2:4246806/1‑100 (MQ=255)

                                                                                 gACAGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCAc                   <  1:1278343/100‑1 (MQ=255)

                                                                                 gACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCAc                   <  2:759873/100‑1 (MQ=255)

                                                                                  ataGTTATGCTGTTCAGTTCAAGCGTCAAGCCTGTTTTATCCTGCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGc                     <  1:2420951/95‑1 (MQ=255)

                                                                                    aGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCa                <  2:934510/100‑1 (MQ=255)

                                                                                    aGTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCa                <  2:309803/100‑1 (MQ=255)

                                                                                    aGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCa                <  2:2492906/100‑1 (MQ=255)

                                                                                     gTCATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCAt               >  2:4253168/1‑100 (MQ=255)

                                                                                       cATGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATcc             >  2:1116360/1‑100 (MQ=255)

                                                                                        aTGCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATcca            <  2:322471/100‑1 (MQ=255)

                                                                                          gCCGTTCAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCACACTCATccac           >  2:1837177/1‑99 (MQ=255)

                                                                                             gttcagttcaAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGc       >  2:2030840/1‑100 (MQ=255)

                                                                                              ttCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGCa      <  1:2828570/100‑1 (MQ=255)

                                                                                              ttCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGCa      <  2:1707214/100‑1 (MQ=255)

                                                                                                cAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGCa      <  1:2720803/98‑1 (MQ=255)

                                                                                                cAGTTCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCCCGCACTCATCCACCAGCATg    >  1:3324271/1‑100 (MQ=255)

GGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTTCTCGCACTCATCCACCAGCATGTC  >  NC_000913/3636880‑3637077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`