Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,701,868 C→A 40.6% R334L (CGT→CTT)  dppF ← dipeptide/heme ABC transporter ATPas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,701,8680CA40.6% 120.9 / 196.0 158R334L (CGT→CTT) dppFdipeptide/heme ABC transporter ATPas
Reads supporting (aligned to +/- strand):  ref base C (59/35);  new base A (39/25);  total (98/60)
Fisher's exact test for biased strand distribution p-value = 8.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

Only 100 of 161 total aligned reads displayed.

GCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGC  >  NC_000913/3701769‑3701964
                                                                                                    |                                                                                                
  cTGGCTGATTCTGCTGTACGGCGTTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGc                                                                                                >  2:682258/1‑100 (MQ=255)
  cTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGc                                                                                                <  2:265098/100‑1 (MQ=255)
  cTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGc                                                                                                <  2:2055487/100‑1 (MQ=255)
     gCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGc                                                                                             >  1:1179041/1‑100 (MQ=255)
     gCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGc                                                                                             <  2:1803786/100‑1 (MQ=255)
      cTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGTCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCg                                                                                            <  2:3002805/100‑1 (MQ=255)
            ctgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAtttt                                                                                      >  1:4078554/1‑100 (MQ=255)
             tgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTc                                                                                     >  2:4178099/1‑100 (MQ=255)
             tgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTc                                                                                     >  1:2936493/1‑100 (MQ=255)
              gctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCa                                                                                    <  1:3387406/100‑1 (MQ=255)
              gctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCa                                                                                    >  2:3351735/1‑100 (MQ=255)
               ctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCAt                                                                                   >  2:2388858/1‑100 (MQ=255)
                 gTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATcc                                                                                 >  1:3254424/1‑100 (MQ=255)
                 gTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATcc                                                                                 <  1:1481319/100‑1 (MQ=255)
                  tACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCt                                                                                >  2:1265422/1‑100 (MQ=255)
                   aCGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTg                                                                               >  2:1428536/1‑100 (MQ=255)
                   aCGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTg                                                                               >  2:1563846/1‑100 (MQ=255)
                    cGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCACATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGa                                                                              >  1:1380378/1‑100 (MQ=255)
                      gCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATc                                                                            <  2:1557750/100‑1 (MQ=255)
                      gCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATc                                                                            >  1:416462/1‑100 (MQ=255)
                      gCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATc                                                                            <  2:347007/100‑1 (MQ=255)
                       cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCa                                                                           <  2:3606824/100‑1 (MQ=255)
                               tATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCaaa                                                                   >  1:3529158/1‑100 (MQ=255)
                               tATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCaaa                                                                   <  2:4108284/100‑1 (MQ=255)
                                 tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAAc                                                                 >  2:727776/1‑100 (MQ=255)
                                  gCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAAc                                                                 >  1:1698509/1‑99 (MQ=255)
                                  gCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACa                                                                >  1:2773647/1‑100 (MQ=255)
                                  gCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACa                                                                >  2:3640759/1‑100 (MQ=255)
                                  gCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACa                                                                >  2:3128297/1‑100 (MQ=255)
                                  gCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTGGTGAGTTAACGCTGCGGATTCTCATCCTGATAAACAGCAAAACa                                                                >  1:649963/1‑100 (MQ=255)
                                   cTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACaa                                                               <  2:1271009/100‑1 (MQ=255)
                                   cTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACaa                                                               >  1:1593883/1‑100 (MQ=255)
                                   cTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACaa                                                               <  1:3246189/100‑1 (MQ=255)
                                     tGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGc                                                             >  2:3306992/1‑100 (MQ=255)
                                      gTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCt                                                            >  2:2216616/1‑100 (MQ=255)
                                          ccACTGTTGAAGCTGATTGAGTAACCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCa                                                        <  1:3127778/100‑1 (MQ=255)
                                          ccACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCa                                                        >  2:285854/1‑100 (MQ=255)
                                          ccACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCa                                                        >  2:2586717/1‑100 (MQ=255)
                                          ccACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCa                                                        >  2:2205607/1‑100 (MQ=255)
                                           cACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAg                                                       >  2:1495119/1‑100 (MQ=255)
                                            aCTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGt                                                      <  2:4229021/100‑1 (MQ=255)
                                             cTGATGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTCTGTGAGATAAAGCAGCGGATTGTCCTCCTGATCAACAGCAAAACAAGCTACCAGt                                                      >  1:105385/1‑99 (MQ=255)
                                              tGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTg                                                    <  1:735581/100‑1 (MQ=255)
                                              tGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTg                                                    <  1:2138026/100‑1 (MQ=255)
                                               gTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGa                                                   >  2:3823303/1‑100 (MQ=255)
                                               gTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGa                                                   >  1:35585/1‑100 (MQ=255)
                                                 tGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAcc                                                 >  2:2256840/1‑100 (MQ=255)
                                                  gAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAccg                                                >  2:3857861/1‑100 (MQ=255)
                                                    aGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAccgcc                                              >  1:3371387/1‑100 (MQ=255)
                                                      cTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGt                                            <  1:3900976/100‑1 (MQ=255)
                                                       taactcacaaaaCCGGGGCCATGCGCTCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTa                                           >  2:1760724/10‑100 (MQ=25)
                                                       tGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTa                                           >  2:425645/1‑100 (MQ=255)
                                                       tGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTa                                           >  1:1794285/1‑100 (MQ=255)
                                                       tGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTa                                           >  2:103184/1‑100 (MQ=255)
                                                       tGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTa                                           >  2:632908/1‑100 (MQ=255)
                                                       tGATTGAGTAAACCGGAGCGAATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTa                                           >  1:2603128/1‑100 (MQ=255)
                                                          ttGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTc                                        >  1:2062993/1‑100 (MQ=255)
                                                           tGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCt                                       >  1:3693138/1‑100 (MQ=255)
                                                           tGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCATAGTCt                                       <  1:194624/100‑1 (MQ=255)
                                                            gAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtt                                      >  2:2725070/1‑100 (MQ=255)
                                                             aGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCttt                                     <  2:259680/100‑1 (MQ=255)
                                                              gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttt                                    <  1:1121921/100‑1 (MQ=255)
                                                              gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttt                                    >  1:2142308/1‑100 (MQ=255)
                                                              gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttt                                    >  1:2984993/1‑100 (MQ=255)
                                                              gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttn                                    <  1:3288808/100‑2 (MQ=255)
                                                              gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATCTTCATCCTGATCAACAGCAAGACGAGCTCGCAGTTGACCGCagtagtaaat                                     >  1:3691806/1‑95 (MQ=255)
                                                              caaaaCCGGGGCCATGCGCTCCGGGTTTGTGAGTTAAAGATGCGGATTTTCATCCGGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTc                                        >  1:1051556/3‑96 (MQ=255)
                                                               tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTa                                   <  2:1504263/100‑1 (MQ=255)
                                                                aaaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAg                                  >  2:2002526/1‑100 (MQ=255)
                                                                aaaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAg                                  <  1:682255/100‑1 (MQ=255)
                                                                 aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGc                                 >  1:4227539/1‑100 (MQ=255)
                                                                 aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGc                                 >  2:320894/1‑100 (MQ=255)
                                                                 aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCTTAGTCTTTTAGc                                 <  2:2809803/100‑1 (MQ=255)
                                                                 aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGGAGTCTTTTAGc                                 >  2:1907966/1‑100 (MQ=255)
                                                                   ccGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTg                               >  2:1226431/1‑100 (MQ=255)
                                                                   ccGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTg                               >  2:756556/1‑100 (MQ=255)
                                                                     ggAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCgg                            >  1:3102125/1‑100 (MQ=255)
                                                                       aGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGc                           >  1:4219098/1‑100 (MQ=255)
                                                                        gcgcATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCt                          >  2:4182900/1‑100 (MQ=255)
                                                                        gcgcATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCt                          >  2:3673700/1‑100 (MQ=255)
                                                                         cgcATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTg                         <  1:2042285/100‑1 (MQ=255)
                                                                           cATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCa                       >  2:1113692/1‑100 (MQ=255)
                                                                                  cccGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTggg                 <  2:1895416/100‑1 (MQ=255)
                                                                                  cccGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTggg                 <  2:2827293/100‑1 (MQ=255)
                                                                                  cccGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTggg                 <  1:578239/100‑1 (MQ=255)
                                                                                  cccGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTggg                 <  1:2716566/100‑1 (MQ=255)
                                                                                  cccGGTTTTGTGAGTCAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTggg                 <  2:4214471/100‑1 (MQ=255)
                                                                                   ccGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAATACAAGCTACCAGTTTGCCGCCGGAGTCTTTTAGCTGCGGCTGCAACTg                   <  1:2364756/97‑1 (MQ=255)
                                                                                   ccGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGt                <  2:2774479/100‑1 (MQ=255)
                                                                                     ggTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGc              >  1:3069088/1‑100 (MQ=255)
                                                                                           gtgCGTTCACGCTCCGGATCTTCATCCTGTTCAACAGCAAAACAAGCTACCAGTTAAACACCGTAGGCATTTAGCTGCGGCTGCAACTgggccccggcgc        >  1:3509201/1‑91 (MQ=255)
                                                                                           gtgAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGc        >  2:706356/1‑100 (MQ=255)
                                                                                           gtgAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGc        >  2:3530611/1‑100 (MQ=255)
                                                                                            tgAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGcc       <  1:2204343/100‑1 (MQ=255)
                                                                                            tgAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGcc       <  2:4078576/100‑1 (MQ=255)
                                                                                            tgAGTTAAAGCTGCGGATTTTCATCTTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGcc       >  2:934745/1‑100 (MQ=255)
                                                                                              aGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGa     >  2:2438574/1‑100 (MQ=255)
                                                                                               gTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGGCCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGaa    <  1:1879078/100‑1 (MQ=255)
                                                                                               gTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGaa    >  2:2949115/1‑100 (MQ=255)
                                                                                                 tAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAgc  >  1:758844/1‑100 (MQ=255)
                                                                                                    |                                                                                                
GCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGC  >  NC_000913/3701769‑3701964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: