Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 162,973 G→A 100% C290Y (TGT→TAT)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913162,9730GA99.6% 521.0 / ‑6.3 154C290Y (TGT→TAT) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (74/79);  total (75/79)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 154 total aligned reads displayed.

TCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGC  >  NC_000913/162877‑163066
                                                                                                |                                                                                             
  ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                          <  2:2993810/100‑1 (MQ=255)
  ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                          <  2:257044/100‑1 (MQ=255)
  ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                          >  2:1561788/1‑100 (MQ=255)
   tccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCa                                                                                         <  2:3451405/100‑1 (MQ=255)
   tccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCa                                                                                         <  1:2763257/100‑1 (MQ=255)
   tccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCa                                                                                         <  1:3990592/100‑1 (MQ=255)
     ccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGTAGGTATTCGTCTGGTGGTGGTTTATGccc                                                                                          >  1:2439739/1‑97 (MQ=255)
         cACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCg                                                                                   >  1:4146909/1‑100 (MQ=255)
         cACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCg                                                                                   <  1:1735223/100‑1 (MQ=255)
           ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtg                                                                                 <  1:1783017/100‑1 (MQ=255)
                 gggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgcgc                                                                           >  2:546527/1‑100 (MQ=255)
                   gATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGt                                                                         >  1:3714606/1‑100 (MQ=255)
                      gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAATCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                      <  1:1590829/100‑1 (MQ=255)
                        gcAAAGTGGTGCTGGCGACTAATATTGCTGAAACCAGTTTAAACATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                    >  1:82023/1‑100 (MQ=255)
                        gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                      >  1:4237635/1‑98 (MQ=255)
                        gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                    <  1:757976/100‑1 (MQ=255)
                        gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                    >  2:1527044/1‑100 (MQ=255)
                          aaaGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGTGCGTGTGGCGCGTTTTGATc                                                                  <  1:149566/100‑1 (MQ=255)
                          aaaGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATc                                                                  <  1:1116480/100‑1 (MQ=255)
                            aGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                                <  2:4212013/100‑1 (MQ=255)
                            aGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                                <  2:854543/100‑1 (MQ=255)
                              tggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                              <  2:2863784/100‑1 (MQ=255)
                              tggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                              <  2:1721721/100‑1 (MQ=255)
                              tggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                              <  1:3498904/100‑1 (MQ=255)
                               ggtgCTGGATACCAATCTTGCTGAAAGCAGTGCAACCATTGAAGATACTCGTCTGGTGGTGGATTATGCCCACGACCTTGTGGCGCGtctg                                                                      >  1:2432409/1‑88 (MQ=255)
                                gtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                            >  2:2027856/1‑100 (MQ=255)
                                  gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGACCAGGACCGTGTGGCGCGTTTTGATCCGCGCAc                                                           >  1:678182/1‑99 (MQ=255)
                                   cTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCgcgc                                                             >  1:1086102/1‑96 (MQ=255)
                                    tGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAAGAGCGTGTGGCGCGTTTTGATCCGCGCACggg                                                        >  1:3002465/1‑100 (MQ=255)
                                     ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgggg                                                       <  1:1584609/100‑1 (MQ=255)
                                     ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGACCCGCGCACgggg                                                       <  2:2800712/100‑1 (MQ=255)
                                       cGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCt                                                     >  1:3744553/1‑100 (MQ=255)
                                        gACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCtt                                                    >  2:1237401/1‑100 (MQ=255)
                                         aCCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTa                                                   <  2:3630132/100‑1 (MQ=255)
                                            aaTATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgc                                                >  2:1404151/1‑100 (MQ=255)
                                            aaTATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgc                                                <  2:3929919/100‑1 (MQ=255)
                                            aaTATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgc                                                <  2:1658346/100‑1 (MQ=255)
                                             atatTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                               <  2:1800234/100‑1 (MQ=255)
                                               atTGGTGAACCCAGTTTACCCATTGAAGGTATTCGTCTGCTGGTGGATTATGCCCAGGGGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGAc                                             <  2:4261856/100‑1 (MQ=255)
                                                 tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                           <  2:2490441/100‑1 (MQ=255)
                                                 tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                           <  1:29008/100‑1 (MQ=255)
                                                 tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                           <  2:4110194/100‑1 (MQ=255)
                                                 tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                           <  2:3609416/100‑1 (MQ=255)
                                                 tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                           <  2:792421/100‑1 (MQ=255)
                                                 tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                           >  1:1204738/1‑100 (MQ=255)
                                                  tcTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAGTATGCCCTGGTGGGTGTGGCGCGGTTTGAGCCGCGTACGGGGCTTCCGCGCCTg                                           >  1:1910400/2‑99 (MQ=255)
                                                  gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                          >  2:2025414/1‑100 (MQ=255)
                                                   cTGAAACCAGTTTAACCATTGAGGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                         >  1:2482881/1‑100 (MQ=255)
                                                   cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGCGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                         >  1:4120082/1‑100 (MQ=255)
                                                    tGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAtt                                        >  2:1100501/1‑100 (MQ=255)
                                                     naaaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTa                                       >  2:235118/2‑100 (MQ=255)
                                                       aaCCAGTTTAGCCATGGAGGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                      <  1:1779048/99‑1 (MQ=255)
                                                       aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCTCGCACGGGGCTTACGCGACTGATTACt                                     >  1:1188336/1‑100 (MQ=255)
                                                       aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                     >  2:3866602/1‑100 (MQ=255)
                                                       aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                     >  2:3898674/1‑100 (MQ=255)
                                                       aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                     >  1:2129368/1‑100 (MQ=255)
                                                       aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                     <  2:3433116/100‑1 (MQ=255)
                                                       aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                     <  2:718480/100‑1 (MQ=255)
                                                       aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                     >  1:3234152/1‑100 (MQ=255)
                                                        aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTc                                    <  2:4099030/100‑1 (MQ=255)
                                                        aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTc                                    >  2:2682158/1‑100 (MQ=255)
                                                         ccggTTTTACCTTTGAAGTTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGCCGCTTTTTGATCGCCGTACCGGGCTTACGCCACTGATTACTCa                                   >  1:1645417/4‑100 (MQ=255)
                                                           aGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAc                                 >  1:3897876/1‑100 (MQ=255)
                                                            gTTTAACGATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                >  2:2242432/1‑100 (MQ=255)
                                                            gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                <  1:478174/100‑1 (MQ=255)
                                                            gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                >  2:3754394/1‑100 (MQ=255)
                                                            gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                <  1:2833079/100‑1 (MQ=255)
                                                             tttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgc                               <  1:2131538/100‑1 (MQ=255)
                                                              ttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                              <  2:704688/100‑1 (MQ=255)
                                                               tAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                             >  1:358437/1‑100 (MQ=255)
                                                                aaCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGtt                            <  1:3235728/100‑1 (MQ=255)
                                                                  ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                          <  2:1491217/100‑1 (MQ=255)
                                                                  ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                          >  2:4259204/1‑100 (MQ=255)
                                                                  ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                          <  1:1217734/100‑1 (MQ=255)
                                                                  ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGGTTTGATCTGCGCACGGTGCTTACGCGACTGATTACTCAACGCGCTAg                          >  1:1790087/1‑100 (MQ=255)
                                                                  ccATTGAAGGTATGCGTCTGGTGGTGGTTTATGCCCAGGAGCGTGTGGGGCGTTTTGATGCGCGGACTGGGCTTACGCGGCTGATTACTGAACGCGTTAg                          >  1:194639/1‑100 (MQ=255)
                                                                   cATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGATTGATTACTCAACGCGTTAGc                         >  2:955614/1‑100 (MQ=255)
                                                                      tGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAg                      <  2:977241/100‑1 (MQ=255)
                                                                      tGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAg                      >  1:227736/1‑100 (MQ=255)
                                                                      tGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTGTGATTCTCGCTCGTGGCTTACGCGACTGATTACTCAACGCGTTAGCCa                       >  1:4058541/1‑99 (MQ=255)
                                                                       gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGGCAg                      >  1:488212/1‑99 (MQ=255)
                                                                       gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                     >  2:1791108/1‑100 (MQ=255)
                                                                         aGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCa                   <  2:2331615/100‑1 (MQ=255)
                                                                         aGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGGTGTGGTTCTCGGACGGGGCTGACGCGTCTGATTACTCAACGCGTTAGCCAGGCa                   >  1:650849/1‑100 (MQ=255)
                                                                          ggTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCa                   <  2:2627888/99‑1 (MQ=255)
                                                                           gTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATc                 >  2:2124256/1‑100 (MQ=255)
                                                                           gTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATc                 <  1:1294289/100‑1 (MQ=255)
                                                                           gTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATc                 <  1:1294997/100‑1 (MQ=255)
                                                                            tATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATcc                <  2:3664188/100‑1 (MQ=255)
                                                                             aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa               <  2:3679192/100‑1 (MQ=255)
                                                                             aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa               <  2:113000/100‑1 (MQ=255)
                                                                             aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa               <  2:1156674/100‑1 (MQ=255)
                                                                               tCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATg             <  2:1953834/100‑1 (MQ=255)
                                                                                 gTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAc           >  1:4120441/1‑100 (MQ=255)
                                                                                    tggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCa        >  1:1501307/1‑100 (MQ=255)
                                                                                     ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAg       >  1:1174893/1‑100 (MQ=255)
                                                                                      gtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGc      >  2:642275/1‑100 (MQ=255)
                                                                                      gtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGc      >  2:689417/1‑100 (MQ=255)
                                                                                        ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGt    >  2:1177837/1‑100 (MQ=255)
                                                                                           ggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGAGCTTAAACGACTGATTACTCAAAGCGTTAGCCAGGCATCCATGACGCAACGTGc  >  1:222449/1‑99 (MQ=255)
                                                                                                |                                                                                             
TCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGC  >  NC_000913/162877‑163066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑