Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,978 A→C 22.6% intergenic (‑188/+120) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9780AC22.6% 676.1 / 187.5 314intergenic (‑188/+120)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base A (107/120);  new base C (59/2);  total (192/122)
Fisher's exact test for biased strand distribution p-value = 2.90e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-02

Only 100 of 320 total aligned reads displayed.

GAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCA  >  NC_000913/3705879‑3706076
                                                                                                   |                                                                                                       
gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                         >  1:2796947/1‑100 (MQ=255)
gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                         >  2:3772715/1‑100 (MQ=255)
 aaGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                                        >  1:3458102/1‑100 (MQ=255)
        gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGAGGCGTTAACGCCTTa                                                                                                 >  2:4205702/1‑100 (MQ=255)
        gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGAAAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                 >  1:2252936/1‑100 (MQ=255)
          tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                               >  2:1922185/1‑100 (MQ=255)
           gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                              >  1:815914/1‑100 (MQ=255)
             cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                            >  2:2086148/1‑100 (MQ=255)
              ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                           >  2:2285025/1‑100 (MQ=255)
               gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                          <  2:3968923/100‑2 (MQ=37)
               gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGGTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                          <  1:3149483/100‑1 (MQ=255)
                aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                         <  2:557144/100‑1 (MQ=255)
                aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                         <  2:646497/100‑3 (MQ=37)
                    cgttGCACGCAGAGGTACAGCGCCCCCGGGTATATAGATTGTTGTCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                     <  1:3858042/96‑1 (MQ=21)
                    cgcTGTTCGCAGAGGCACAGCGCTACCGGGTATACAGATTGGTGACAAAGTGCGCTTTGTTTATTCCGGATAAGGCGTTATCGCCGCATCCGGCATaaac                                                                                     <  1:1002361/100‑4 (MQ=21)
                     gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                   >  1:1268005/1‑100 (MQ=255)
                       tGCAGGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                 <  1:2319343/100‑1 (MQ=255)
                       tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                 <  2:1124123/100‑1 (MQ=255)
                          aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                              >  2:1196977/1‑100 (MQ=255)
                           cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                             <  2:1050710/100‑1 (MQ=255)
                           cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                             <  1:401646/100‑1 (MQ=255)
                           cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                             <  1:1867245/100‑1 (MQ=255)
                            ggagagGCACAGCACCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                            >  2:2876369/3‑100 (MQ=255)
                            gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                            <  2:1994244/100‑1 (MQ=255)
                                 ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                       >  1:2217753/1‑100 (MQ=255)
                                 gacacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                       >  2:3654339/3‑100 (MQ=255)
                                   cacgGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                     >  2:1513226/1‑100 (MQ=255)
                                    acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                                    <  1:2889123/100‑1 (MQ=255)
                                     caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                   <  1:505650/100‑1 (MQ=255)
                                     caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                   <  1:2854196/100‑1 (MQ=255)
                                           cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                             >  2:1687148/1‑100 (MQ=255)
                                           cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                             <  2:2369967/100‑1 (MQ=255)
                                            aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                            <  2:2502954/100‑1 (MQ=255)
                                              cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                         <  1:3031665/100‑1 (MQ=255)
                                              cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                         <  1:3330410/100‑1 (MQ=255)
                                                      cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCg                                              <  2:2109352/100‑1 (MQ=255)
                                                        gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTa                                            <  2:1379094/100‑1 (MQ=255)
                                                          atcttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAgg                                          <  1:1686241/97‑1 (MQ=255)
                                                            gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGcc                                        >  1:727115/1‑100 (MQ=255)
                                                             ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCt                                       >  1:1478996/1‑100 (MQ=255)
                                                               gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGa                                     <  1:3536186/100‑1 (MQ=255)
                                                               gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGa                                     >  2:2911931/1‑100 (MQ=255)
                                                                 cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATa                                   >  2:3252168/1‑100 (MQ=255)
                                                                     gTGCGCTTTGTTTATGCCGGATGTGGCGTTAACTCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCg                               <  2:1842329/100‑1 (MQ=255)
                                                                     gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCg                               >  2:2175300/1‑100 (MQ=255)
                                                                      tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCgg                              >  1:2823462/1‑100 (MQ=255)
                                                                      tGCGCGTTGTTTATGCCGGATTCGGAGTTATCGGCTTATCTGGCCTACAAAATCGTGCAAACTCAAGACATTGCAGAATTTTCGTAGGCCTGATAAGCgg                              <  1:2624705/100‑1 (MQ=18)
                                                                         gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgc                           <  1:1567754/100‑1 (MQ=255)
                                                                         gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgc                           <  1:1911322/100‑1 (MQ=255)
                                                                          cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgcg                          <  1:1531116/100‑1 (MQ=255)
                                                                          cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgcg                          >  2:289564/1‑100 (MQ=255)
                                                                          cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgcg                          <  1:2047092/100‑1 (MQ=255)
                                                                          cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgcg                          <  2:1782461/100‑1 (MQ=255)
                                                                            ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCa                        >  2:3497768/1‑100 (MQ=255)
                                                                            atttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTTGTAGGCCTGATAAGCGTAGCGCAt                       >  1:3903465/9‑100 (MQ=18)
                                                                             tttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCAt                       >  2:27686/8‑100 (MQ=18)
                                                                             tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCAt                       >  1:1600225/1‑100 (MQ=255)
                                                                             tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCAt                       >  1:2553322/1‑100 (MQ=255)
                                                                              gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATc                      >  2:2683847/1‑100 (MQ=255)
                                                                               tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCa                     >  1:639892/6‑100 (MQ=17)
                                                                                gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAATTCTGCAAATTCAACAAATTGCGAATCCCTTGTAGGCCTGATAAGCGTAGCGCATCAgg                   >  2:679164/5‑100 (MQ=18)
                                                                                 taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCATGTAGGCCTGATAAGCGTAGCGCATCAgg                   >  2:251099/4‑100 (MQ=18)
                                                                                 taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGATCATGTAGGCCTGATAAGCGTAGCGCATCAgg                   >  2:271092/4‑100 (MQ=17)
                                                                                 tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAGATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAgg                   >  1:2997110/1‑100 (MQ=255)
                                                                                  aggCCGGATAAGGCGTTTACGCCGCACCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCACGTAGGCCTGATAAGCGTAGCGCATCAGGc                  >  2:2596725/3‑100 (MQ=17)
                                                                                  aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCATGTAGGCCTGATAAGCGTAGCGCATCAGGc                  >  2:384718/3‑100 (MQ=18)
                                                                                  aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGCAGGAAACACGTAGGCCTGATAAGCGAAGCGCATCAGGc                  >  1:955708/3‑100 (MQ=18)
                                                                                  aggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGc                  >  2:3035489/3‑100 (MQ=18)
                                                                                  aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGc                  <  1:1432903/100‑1 (MQ=255)
                                                                                  aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGc                  <  1:1191859/100‑1 (MQ=255)
                                                                                   ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTCTAGGCCTGATAAGCGTAGCGCATCAGGCa                 >  1:2564641/2‑100 (MQ=17)
                                                                                   cgctggataaggcgTTCACGCAGCATCCAGCCTACAAAATTGTGCAAAATCAATGGATTGCACATTTAGCGTAGGCCTGATAAGCGTAGCGCATCAGGCa                 >  1:364030/11‑100 (MQ=18)
                                                                                    gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCa                 >  1:3434330/1‑99 (MQ=255)
                                                                                    gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt                >  2:3743114/1‑100 (MQ=255)
                                                                                    gCCGGATAAGGCGTTTACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCag                >  2:4055131/1‑99 (MQ=18)
                                                                                    gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCACGTAGGCCTGATAAGCGTAGCGCATCAGGCag                >  1:2953754/1‑99 (MQ=17)
                                                                                    gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCaa                >  2:3164096/1‑99 (MQ=25)
                                                                                     ccggataaggcgTTTACGCCGCATCCGGTCTACAAAACAGTGCAAATTCAATATATTGCAATCTTCTGGTAGGCCTGATAAGCATCGCGCATCAGGCaag               >  2:15831/9‑98 (MQ=18)
                                                                                     ccggataaggcgTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAtt               >  2:1889770/9‑100 (MQ=18)
                                                                                      cggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCACGTAGGCCTGATAAGCGTAGCGCATCAGGCAgtt              >  1:1786424/8‑100 (MQ=17)
                                                                                      cggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCATGTAGGCCTGATAAGCGTAGCGCATCAGGCagtt              >  2:3410148/8‑97 (MQ=18)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt             >  1:1127275/7‑100 (MQ=18)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt             >  1:3477226/7‑100 (MQ=18)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAattc             >  2:1327207/7‑99 (MQ=21)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAattc             >  1:3066090/7‑99 (MQ=21)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAattt             >  2:494128/7‑100 (MQ=17)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAattt             >  1:1320148/7‑100 (MQ=17)
                                                                                       ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt             <  1:1960470/100‑1 (MQ=255)
                                                                                       ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt             >  2:3306813/1‑100 (MQ=255)
                                                                                        gataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCagctta            >  2:3466834/6‑95 (MQ=21)
                                                                                        gataacgcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCCGAAACCCTGTAGGCCTCAACAGCGGCGCGGATCACGc                  >  1:2085452/7‑94 (MQ=9)
                                                                                         aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg           <  2:4112468/100‑1 (MQ=255)
                                                                                         aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg           <  2:2629454/100‑1 (MQ=255)
                                                                                          taaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCACGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttgc          >  1:4078602/4‑100 (MQ=17)
                                                                                          tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAGGCGGAGCGCATCAGGCATTtttgc          >  2:1977453/1‑100 (MQ=255)
                                                                                          tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc          >  2:2425261/1‑100 (MQ=255)
                                                                                           gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACACTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc         <  1:3745252/100‑1 (MQ=255)
                                                                                                gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc    >  2:771379/1‑100 (MQ=255)
                                                                                                 ttAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc    <  2:871169/99‑1 (MQ=255)
                                                                                                  tAACGCCTTATCCGGCCTACAAAATCGTACAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa  >  2:4273223/1‑100 (MQ=255)
                                                                                                   |                                                                                                       
GAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCA  >  NC_000913/3705879‑3706076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: