Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,802,190 T→C 38.3% K290R (AAA→AGA)  waaO ← UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑ alpha‑1,3‑D‑galactosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,802,1900TC38.3% 138.4 / 192.2 183K290R (AAA→AGA) waaOUDP‑D‑galactose:(glucosyl)lipopolysaccharide‑ alpha‑1,3‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (49/64);  new base C (40/30);  total (89/94)
Fisher's exact test for biased strand distribution p-value = 9.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 191 total aligned reads displayed.

AGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGTTGGTTA  >  NC_000913/3802091‑3802289
                                                                                                   |                                                                                                   
aGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCAtttt                                                                                                     >  2:1327238/1‑100 (MQ=255)
   tatCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTg                                                                                                  >  1:1402691/1‑100 (MQ=255)
   tatCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTg                                                                                                  <  2:1983971/100‑1 (MQ=255)
    atCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGc                                                                                                 <  2:768400/100‑1 (MQ=255)
     tCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGcc                                                                                                >  2:4148986/1‑100 (MQ=255)
       tATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTc                                                                                              >  2:217376/1‑100 (MQ=255)
       tATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTc                                                                                              >  1:3733612/1‑100 (MQ=255)
         tGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCa                                                                                            <  1:1227897/100‑1 (MQ=255)
           cTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATa                                                                                          >  2:328938/1‑100 (MQ=255)
           cTTTTTAAGAATATATTTTGCGCTATATCTTAATTGATTGTTATTGTTCGGTTTCAGCAACGACATATTATTCCATAGATAAGCATTTTTAGCCTCCATa                                                                                          >  1:3290430/1‑100 (MQ=255)
             ttttAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATa                                                                                          >  1:3327474/1‑98 (MQ=255)
             ttttAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATaaa                                                                                        <  1:260792/100‑1 (MQ=255)
              tttAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTTGGTTTCAGCAACGCCGTATTCTTCTATGGCGAAGCATTTCTTGCCTCCATa                                                                                          >  1:2471006/1‑97 (MQ=255)
              tttAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAg                                                                                       <  2:3095291/100‑1 (MQ=255)
              tttAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAg                                                                                       <  1:3295444/100‑1 (MQ=255)
                tAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCATCCATAAAGGc                                                                                     >  1:2124663/1‑100 (MQ=255)
                  aGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCtt                                                                                   >  1:830307/1‑100 (MQ=255)
                   gCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTtg                                                                                  <  1:4020451/100‑1 (MQ=255)
                    cATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTtgt                                                                                 >  2:455849/1‑100 (MQ=255)
                    cATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTtgt                                                                                 <  1:252126/100‑1 (MQ=255)
                    cATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTtgt                                                                                 <  2:2766362/100‑1 (MQ=255)
                    cATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTtgt                                                                                 <  2:1108838/100‑1 (MQ=255)
                    cATATGTTTTGCGCTATATCCTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTtgt                                                                                 >  1:2616085/1‑100 (MQ=255)
                      tatGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGa                                                                               <  1:3177832/100‑1 (MQ=255)
                      tatGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGa                                                                               <  2:54666/100‑1 (MQ=255)
                      tatGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGa                                                                               <  1:904315/100‑1 (MQ=255)
                         gTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATAc                                                                            >  2:4187696/1‑100 (MQ=255)
                          ttttGTACTATATCTTACTTGATTCCCATCCTAAGGTTCCAACAACGCCGTATTCTTCCATGGCAAAGCATTTCTTGCCTCCATACAGGCTTGTGa                                                                               <  1:3839285/96‑1 (MQ=255)
                          ttttGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACa                                                                           <  1:3868878/100‑1 (MQ=255)
                           tttGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAg                                                                          <  2:1290275/100‑1 (MQ=255)
                           tttGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAg                                                                          <  2:176324/100‑1 (MQ=255)
                            ttGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAgg                                                                         <  1:1572929/100‑1 (MQ=255)
                            ttGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAgg                                                                         <  1:2443469/100‑1 (MQ=255)
                             tGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGa                                                                        <  2:2107876/100‑1 (MQ=255)
                             tGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGa                                                                        >  1:1712144/1‑100 (MQ=255)
                              gcgcTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGAt                                                                       <  1:1361988/100‑1 (MQ=255)
                                 cTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAAt                                                                    >  1:1031798/1‑100 (MQ=255)
                                  tataTCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATc                                                                   <  2:789417/100‑1 (MQ=255)
                                   atatCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATcc                                                                  <  2:2391863/100‑1 (MQ=255)
                                    tatCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATccc                                                                 <  2:3615395/100‑1 (MQ=255)
                                     atCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCa                                                                <  1:2714699/100‑1 (MQ=255)
                                      tCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGAGACAGGATAATCCCAg                                                               >  2:4211837/1‑100 (MQ=255)
                                      tCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAg                                                               >  1:2060243/1‑100 (MQ=255)
                                         tAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGcc                                                            >  2:3981708/1‑100 (MQ=255)
                                          aaTTGATTGCTATTGTTCGTTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGccc                                                           <  1:2827231/100‑1 (MQ=255)
                                           attgattgCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTTTTTTTTTATAGGCTTGTGATACAGGATAATCCCa                                                                >  1:3151991/1‑94 (MQ=255)
                                             tgattgCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCGCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAAt                                                        <  1:1524111/100‑1 (MQ=255)
                                               attgCTATTGTTCGTTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCa                                                      <  1:3537244/100‑1 (MQ=255)
                                                 tgCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATg                                                    <  2:145938/100‑1 (MQ=255)
                                                 tgCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATg                                                    >  1:3536339/1‑100 (MQ=255)
                                                 ngCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATg                                                    >  1:1767924/2‑100 (MQ=255)
                                                  gCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGc                                                   <  2:1555106/100‑1 (MQ=255)
                                                  gCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGc                                                   >  2:3243919/1‑100 (MQ=255)
                                                  gCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGc                                                   >  2:4106907/1‑100 (MQ=255)
                                                   cTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGcc                                                  >  1:1854571/1‑100 (MQ=255)
                                                      ttGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAgg                                               >  2:2849358/1‑100 (MQ=255)
                                                      ttGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAgg                                               >  2:1229746/1‑100 (MQ=255)
                                                       tGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAggg                                              <  1:1680638/100‑1 (MQ=255)
                                                       tGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAggg                                              <  2:2818750/100‑1 (MQ=255)
                                                        gTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGc                                             >  2:1530849/1‑100 (MQ=255)
                                                         ttCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCt                                            >  2:3887879/1‑100 (MQ=255)
                                                          tCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCtt                                           <  2:375487/100‑1 (MQ=255)
                                                           cGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttg                                          >  1:2919055/1‑100 (MQ=255)
                                                            ggTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttgg                                         <  1:1455353/100‑1 (MQ=255)
                                                             gattgaGCCACGACGTTTTTTTCCTGCGCGAAGCCTTTTTTTCCTCCCTAATGGCTTATGATACACTATTATCCCAGGCCCAATCATAGCAGGGCttg                                          >  1:612594/6‑98 (MQ=255)
                                                              tttCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttggtt                                       >  1:1007086/1‑100 (MQ=255)
                                                              tttCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttggtt                                       <  1:3011217/100‑1 (MQ=255)
                                                              tttCAGCAACGCCGTATTCTTCAATGGCGAAGCATTTCTTGCCTCCATAAGGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttggt                                        <  1:3599650/99‑1 (MQ=255)
                                                                tCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttggttgg                                     >  2:3176950/1‑100 (MQ=255)
                                                                  aGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGcc                                   >  1:4191456/1‑100 (MQ=255)
                                                                   ggaaCGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGccc                                  <  1:4132932/98‑1 (MQ=255)
                                                                   gCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTTTTTCTATAAATTTTTTTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGccc                                  >  2:2303036/1‑100 (MQ=255)
                                                                   gCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGccc                                  >  2:2454523/1‑100 (MQ=255)
                                                                   gCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGccc                                  <  2:4128403/100‑1 (MQ=255)
                                                                    cAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCg                                 <  2:3481834/100‑1 (MQ=255)
                                                                      aCGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGat                               >  1:196927/1‑100 (MQ=255)
                                                                        gCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGAGACAGGATAATCCCAGGCCCAACCAAGCCCTGGCatgattggggctggg                              <  1:517911/99‑8 (MQ=25)
                                                                        gCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGatat                             >  1:3675619/1‑100 (MQ=255)
                                                                         ccGTATTCTTCCATGGCGAAGCATTTTTTTTTTATTTTTTGACTTGTGATACAGGATAATTTAAGGCCTTATCATTTCGGGGCTTGGTTGGCCCGTtata                            >  1:2565410/1‑100 (MQ=255)
                                                                          cGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATaa                           <  2:1007089/100‑1 (MQ=255)
                                                                            tATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATg                         >  1:1026399/1‑100 (MQ=255)
                                                                            tATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATg                         <  1:4189013/100‑1 (MQ=255)
                                                                              ttcttcCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGa                       <  2:1667674/100‑1 (MQ=255)
                                                                                cttcCATGGCGAAGCATTTTTTTCTTTTTTTTTATCTTGTAATACGGGATAATCCTAGCCCCATTCATGCCAGGGCTTGGTTGGGCCGTTATAATGTAt                      >  1:1743144/1‑99 (MQ=255)
                                                                                  tcCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATaaa                   <  2:3024580/100‑1 (MQ=255)
                                                                                  tcCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATaaa                   <  1:2432717/100‑1 (MQ=255)
                                                                                  tcCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATaaa                   >  2:1902038/1‑100 (MQ=255)
                                                                                     aTGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAAt                <  2:1023605/100‑1 (MQ=255)
                                                                                      tGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATa               >  2:3394849/1‑100 (MQ=255)
                                                                                       ggCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAg              <  2:1059759/100‑1 (MQ=255)
                                                                                       ggCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAg              >  1:3550538/1‑100 (MQ=255)
                                                                                       ggCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAg              <  2:3151700/100‑1 (MQ=255)
                                                                                        gCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGt             >  2:3589768/1‑100 (MQ=255)
                                                                                            aGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCg         <  2:4263718/100‑1 (MQ=255)
                                                                                            aGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCg         >  2:2733301/1‑100 (MQ=255)
                                                                                             gCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGt        >  2:2504810/1‑100 (MQ=255)
                                                                                               aTTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGtt       >  2:59513/1‑99 (MQ=255)
                                                                                                ttttttGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGTTgg     >  2:2914443/1‑100 (MQ=255)
                                                                                                  ttttGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGTTGGtt   <  2:2282244/100‑1 (MQ=255)
                                                                                                   tttGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGTTGGTTa  <  1:3707533/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AGATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGTTGGTTA  >  NC_000913/3802091‑3802289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: