Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 531,655 C→A 31.7% A189A (GCG→GCT allS ← allD operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913531,6550CA31.7% 240.1 / 154.9 173A189A (GCG→GCTallSallD operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (62/56);  new base A (29/26);  total (91/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01

Only 100 of 177 total aligned reads displayed.

ACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATT  >  NC_000913/531557‑531753
                                                                                                  |                                                                                                  
aCAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGACGGAAAGCGCCGCAACTgcg                                                                                                   >  1:3587539/1‑100 (MQ=255)
 cAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTgcgc                                                                                                  >  2:4012296/1‑100 (MQ=255)
 cAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTgcgc                                                                                                  <  2:1964689/100‑1 (MQ=255)
  aataatCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCt                                                                                                 <  2:3581477/100‑1 (MQ=255)
   ataatCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCtt                                                                                                >  2:1781702/1‑100 (MQ=255)
    taatCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTc                                                                                               <  2:2657361/100‑1 (MQ=255)
      atCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTg                                                                                             <  1:4149661/100‑1 (MQ=255)
        ctctTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGtt                                                                                           <  2:524318/100‑1 (MQ=255)
        ctctTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGtt                                                                                           <  2:1048617/100‑1 (MQ=255)
        ctctTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGtt                                                                                           <  1:91805/100‑1 (MQ=255)
           tttttGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGc                                                                                        <  1:3877444/100‑1 (MQ=255)
            ttttGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGc                                                                                        <  1:703322/99‑1 (MQ=255)
            ttttGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCg                                                                                       <  1:3688773/100‑1 (MQ=255)
             cgtcgccTGGCGATTGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCgg                                                                                      >  1:1296768/6‑100 (MQ=255)
              ttGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGGGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGc                                                                                     <  1:3459212/100‑1 (MQ=255)
                gCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGctc                                                                                   >  1:3728176/1‑100 (MQ=255)
                 cccTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGctct                                                                                  >  2:3725078/1‑100 (MQ=255)
                 cccTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGctct                                                                                  >  2:964037/1‑100 (MQ=255)
                 cccTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGctct                                                                                  >  1:2004014/1‑100 (MQ=255)
                  ccTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCtt                                                                                 >  2:4215766/1‑100 (MQ=255)
                  ccTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCtt                                                                                 >  1:462041/1‑100 (MQ=255)
                   cTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGCCCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTc                                                                                <  1:320460/100‑1 (MQ=255)
                   cTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTc                                                                                >  2:1840874/1‑100 (MQ=255)
                    tGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCa                                                                               >  2:3530967/1‑100 (MQ=255)
                     ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCaa                                                                              >  2:3407654/1‑100 (MQ=255)
                     ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCa                                                                               <  1:3806823/99‑1 (MQ=255)
                      gCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAAc                                                                             <  2:1685900/100‑1 (MQ=255)
                       cAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACg                                                                            <  1:3180976/100‑1 (MQ=255)
                       cAATCGCCAGGCGACGCGCTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACGGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCaa                                                                              >  1:3524530/1‑98 (MQ=255)
                          tCGCCAGGCGACGCGTTTGGTTAAGGTGCGAGCACTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTATTCAACGTTc                                                                         >  1:2810114/1‑100 (MQ=255)
                           cGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCg                                                                        >  2:3433547/1‑100 (MQ=255)
                            gCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGc                                                                       <  2:3911664/100‑1 (MQ=255)
                            gCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGc                                                                       >  1:1334048/1‑100 (MQ=255)
                             ccAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGcc                                                                      <  1:4215744/100‑1 (MQ=255)
                             ccAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGcc                                                                      >  2:1571529/1‑100 (MQ=255)
                              cAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCa                                                                     >  2:2856353/1‑100 (MQ=255)
                                ggCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGc                                                                   <  2:789941/100‑1 (MQ=255)
                                ggCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGc                                                                   >  2:2439603/1‑100 (MQ=255)
                                 gCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCg                                                                  <  2:4095306/100‑1 (MQ=255)
                                    aCGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGgat                                                               <  1:281391/100‑1 (MQ=255)
                                         tttGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATcc                                                          <  2:3755636/100‑1 (MQ=255)
                                           tGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgn                                                        <  1:669900/100‑2 (MQ=255)
                                            ggTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgcc                                                       >  2:92718/1‑100 (MQ=255)
                                             gTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgccg                                                      >  2:444206/1‑100 (MQ=255)
                                             gTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgccg                                                      <  1:784901/100‑1 (MQ=255)
                                              ttAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCcgccgc                                                     <  2:688261/100‑1 (MQ=255)
                                               tAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCgccgcc                                                    <  2:2366017/100‑1 (MQ=255)
                                               tAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATCATccgctggc                                                    <  1:1764189/100‑5 (MQ=255)
                                                aaGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCa                                                   <  2:1789771/100‑1 (MQ=255)
                                                  ggTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCAt                                                  >  1:1504550/1‑99 (MQ=255)
                                                  ggTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATg                                                 >  1:1504408/1‑100 (MQ=255)
                                                  ggTGCGGGCGCTGTCTTCAATATTCACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTCGCGGCTCCTCACCGTTCGCCAGCGGATGATCCgccgccc                                                   >  1:1873289/1‑97 (MQ=255)
                                                    tGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGAc                                               >  1:1747485/1‑100 (MQ=255)
                                                     gCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACa                                              >  2:1000017/1‑100 (MQ=255)
                                                      cGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACaa                                             >  2:3244708/1‑100 (MQ=255)
                                                       gggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACaaa                                            >  2:3016090/1‑100 (MQ=255)
                                                        ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAg                                           <  1:2706354/100‑1 (MQ=255)
                                                        ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAg                                           >  2:3203538/1‑100 (MQ=255)
                                                          cgcTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAgcg                                         <  1:1077505/100‑1 (MQ=255)
                                                          cgcTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAgcg                                         >  1:2558924/1‑100 (MQ=255)
                                                          cgcTGGCTTCAAGATGGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAgcg                                         <  1:3790743/100‑1 (MQ=255)
                                                                cTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCAtt                                    >  1:224942/1‑99 (MQ=255)
                                                                cTTCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTg                                   >  2:1196976/1‑100 (MQ=255)
                                                                 ttCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGc                                  >  2:929992/1‑100 (MQ=255)
                                                                  tCAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCa                                 >  2:2463451/1‑100 (MQ=255)
                                                                  tCAAGATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTGCGCCAGCGGATGATCCGCCGCCATGACAAGGCGCCATTGCa                                 >  2:2326142/1‑100 (MQ=255)
                                                                   cAATATTGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGc                                  >  2:851485/1‑98 (MQ=255)
                                                                    aaTATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAcc                               >  2:359341/1‑100 (MQ=255)
                                                                       atTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGAt                            >  2:3352492/1‑100 (MQ=255)
                                                                        ttGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATc                           <  1:2152146/100‑1 (MQ=255)
                                                                        ttGACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATc                           <  1:3245429/100‑1 (MQ=255)
                                                                         tGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATcc                          <  1:2696912/100‑1 (MQ=255)
                                                                         tGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATcc                          >  1:2163380/1‑100 (MQ=255)
                                                                          gACCGCCGGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCt                         >  2:2080331/1‑100 (MQ=255)
                                                                           aCCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTa                        >  2:594371/1‑100 (MQ=255)
                                                                            ccgccgGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTaa                       >  1:244112/1‑100 (MQ=255)
                                                                              gccgGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCCGCGGCTGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTaatg                     >  1:165314/1‑98 (MQ=255)
                                                                              gccgGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAgg                     <  2:665793/100‑1 (MQ=255)
                                                                              gccgGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAgg                     >  1:2385569/1‑100 (MQ=255)
                                                                              gccgGAAAGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAgg                     >  1:2652756/1‑100 (MQ=255)
                                                                               ccgGAAAGGGCCGCAACTCCGCTTCTGTTAGCGGCTCTTCAACGTGCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAggg                    <  1:3341866/100‑1 (MQ=255)
                                                                               ccgGAAAGCGCCGCACCTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAgg                     <  1:3642409/99‑1 (MQ=255)
                                                                               ccgGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAggg                    <  1:509005/100‑1 (MQ=255)
                                                                                    aaGCGCCGCAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCGGCCGCCATGACAACGCGCCATTGCACCGATCCTAAGGGATCaa               >  1:2055697/1‑100 (MQ=255)
                                                                                     aGCGCCACAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAg              >  1:229686/1‑100 (MQ=255)
                                                                                       cgccgcAACTGCGCTTCTGTTGGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGAc            <  1:2916234/100‑1 (MQ=255)
                                                                                       cgccgcAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGAc            <  1:4164937/100‑1 (MQ=255)
                                                                                       cgccgcAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGAc            >  2:1215291/1‑100 (MQ=255)
                                                                                        gccgcAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACt           >  1:2096428/1‑100 (MQ=255)
                                                                                        gccgcAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACt           >  2:575345/1‑100 (MQ=255)
                                                                                        gccgcAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACt           >  2:2007168/1‑100 (MQ=255)
                                                                                        gccgcAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGaat           >  1:1795577/1‑98 (MQ=255)
                                                                                         ccgcAACTGAGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTa          <  2:1747490/100‑1 (MQ=255)
                                                                                            cAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGTTGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAg       <  1:2993463/100‑1 (MQ=255)
                                                                                            cAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAg       <  1:2746211/100‑1 (MQ=255)
                                                                                            cAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAg       <  2:3830562/100‑1 (MQ=255)
                                                                                             aaCTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAgg      <  1:835058/100‑1 (MQ=255)
                                                                                               cTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGGTGATCCGCCGCCATGCCAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAgg      >  1:1277030/1‑98 (MQ=255)
                                                                                               cTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTa    <  1:1532351/100‑1 (MQ=255)
                                                                                                 gcgcTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCCCCGATCCTAAGGGATCAAGACTAAAGGTAtt  >  1:4127896/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
ACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATT  >  NC_000913/531557‑531753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: