Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,721 G→T 47.5% C51* (TGC→TGA)  ybcJ ← ribosome‑associated protein; putative RNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,7210GT47.5% 37.3 / 237.1 156C51* (TGC→TGA) ybcJribosome‑associated protein; putative RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (44/38);  new base T (46/28);  total (90/66)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 162 total aligned reads displayed.

AAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTC  >  NC_000913/556622‑556820
                                                                                                   |                                                                                                   
aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTg                                                                                                     <  1:1720647/100‑1 (MQ=255)
  aaGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCa                                                                                                   >  2:3968571/1‑100 (MQ=255)
   agagAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAg                                                                                                  <  1:3642715/100‑1 (MQ=255)
   agagAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAg                                                                                                  >  2:3066359/1‑100 (MQ=255)
        aCCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGttt                                                                                             >  2:2185622/1‑100 (MQ=255)
         ccGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTg                                                                                            >  2:541194/1‑100 (MQ=255)
         ccGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTg                                                                                            >  2:467364/1‑100 (MQ=255)
         ccGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTg                                                                                            <  1:2823969/100‑1 (MQ=255)
           ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgcg                                                                                          >  1:3357280/1‑100 (MQ=255)
            gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCAGCGACGATTTTGCAGCGTTTccgc                                                                                         >  1:2641675/1‑100 (MQ=255)
             ccTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGcgcg                                                                                        <  1:1711443/100‑1 (MQ=255)
             ccTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGcgcg                                                                                        <  2:715707/100‑1 (MQ=255)
               tGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGtt                                                                                      >  1:523570/1‑100 (MQ=255)
               tGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGtt                                                                                      >  2:83726/1‑100 (MQ=255)
                gTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGttt                                                                                     <  1:3219629/100‑1 (MQ=255)
                  tGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCa                                                                                   >  2:612442/1‑100 (MQ=255)
                   gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCaa                                                                                  <  1:1531859/100‑1 (MQ=255)
                    aTACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAAc                                                                                 <  2:1107496/100‑1 (MQ=255)
                    aTACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAAc                                                                                 >  2:3467018/1‑100 (MQ=255)
                    aTACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAAc                                                                                 <  2:3794990/100‑1 (MQ=255)
                      aCAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCg                                                                               <  2:2445664/100‑1 (MQ=255)
                      aCAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCg                                                                               >  1:436785/1‑100 (MQ=255)
                       cAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGc                                                                              >  2:1670743/1‑100 (MQ=255)
                       cAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTAACTGTCTGACCGTCCACGATTTTGCAGCGTTTGCGCGTTTCAACCGc                                                                              >  1:1793325/1‑100 (MQ=255)
                         ggCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCAc                                                                            <  1:246636/100‑1 (MQ=255)
                            cGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGt                                                                         <  2:2312857/100‑1 (MQ=255)
                              ggAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCg                                                                       >  1:3873054/1‑100 (MQ=255)
                              ggAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCg                                                                       <  2:294379/100‑1 (MQ=255)
                              ggAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCg                                                                       <  1:1401399/100‑1 (MQ=255)
                                aaaGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGAc                                                                     <  1:1113084/100‑1 (MQ=255)
                                 aaGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACt                                                                    >  2:4016455/1‑100 (MQ=255)
                                   gggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACttt                                                                  >  1:582599/1‑100 (MQ=255)
                                   gggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACttt                                                                  >  1:396384/1‑100 (MQ=255)
                                   gggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACttt                                                                  >  2:2044344/1‑100 (MQ=255)
                                    ggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTc                                                                 <  2:3989007/100‑1 (MQ=255)
                                    ggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTc                                                                 >  2:2677418/1‑100 (MQ=255)
                                    ggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTc                                                                 <  1:1631637/100‑1 (MQ=255)
                                     gATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCa                                                                >  2:1280195/1‑100 (MQ=255)
                                      aTCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAc                                                               >  2:3139207/1‑100 (MQ=255)
                                        cAGGCAACAACCTGTACGCTGTGACCTGCAACACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCt                                                             <  1:2463149/100‑1 (MQ=255)
                                         aGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTg                                                            <  2:4110575/100‑1 (MQ=255)
                                         aGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTg                                                            >  2:1146142/1‑100 (MQ=255)
                                          ggCAACAACCTGTACGCTGTTACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTg                                                            <  1:1244056/99‑1 (MQ=255)
                                          ggCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACTGCACCGTCGACTTTCACCTg                                                            >  1:2044196/1‑99 (MQ=255)
                                          ggCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTgg                                                           >  2:3674731/1‑100 (MQ=255)
                                          ggCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTgg                                                           <  1:1084448/100‑1 (MQ=255)
                                           gCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGc                                                          <  1:83726/100‑1 (MQ=255)
                                            caacaaCCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGcc                                                         >  1:4142111/1‑100 (MQ=255)
                                              acaacCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTgg                                                           <  1:1873324/96‑1 (MQ=255)
                                                  ccTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTTTTTTTTTTTTCGGTGCAACCGCACCGTCGACTTTTACCTGGCCTTCgg                                                    >  1:1420100/1‑99 (MQ=255)
                                                  ccTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                                                   <  2:3155808/100‑1 (MQ=255)
                                                  ccTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                                                   >  1:1500591/1‑100 (MQ=255)
                                                  ccTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                                                   <  1:2219898/100‑1 (MQ=255)
                                                  ccTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                                                   <  1:233009/100‑1 (MQ=255)
                                                  ccTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                                                   <  1:2970230/100‑1 (MQ=255)
                                                          cTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGa                                           >  1:2779785/1‑100 (MQ=255)
                                                          cTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGa                                           >  1:659223/1‑100 (MQ=255)
                                                           tgtgACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAt                                          >  1:308456/1‑100 (MQ=255)
                                                               aCCTGCAAAACTCACTGTCTGACCTGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTc                                      <  1:300882/100‑1 (MQ=255)
                                                               aCCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCTCCTGGCCTTCGGCAATCGCGATTTTc                                      <  2:2591712/100‑1 (MQ=255)
                                                                ccTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCg                                     <  2:1192334/100‑1 (MQ=255)
                                                                 cTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGCCTTTCACCTGGCCTTCGGCAATCGCGATTTTCGc                                    >  1:1575575/1‑100 (MQ=255)
                                                                 cTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGc                                    >  1:860053/1‑100 (MQ=255)
                                                                 cTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGc                                    >  2:328551/1‑100 (MQ=255)
                                                                   gCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCt                                  >  1:4223776/1‑100 (MQ=255)
                                                                      aaaCTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcg                               >  2:1049383/1‑100 (MQ=255)
                                                                      aaaCTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcg                               >  1:164530/1‑100 (MQ=255)
                                                                       aaCGCACTGTCTGACCGGGGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcgc                              <  1:2837412/100‑1 (MQ=255)
                                                                        aCTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcgcg                             >  1:2443166/1‑100 (MQ=255)
                                                                        aCTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcgcg                             <  2:2779796/100‑1 (MQ=255)
                                                                        aCTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcgcg                             >  1:1330421/1‑100 (MQ=255)
                                                                         cTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcgcgc                            <  1:2658245/100‑1 (MQ=255)
                                                                         cTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcgcgc                            >  1:4201609/1‑100 (MQ=255)
                                                                          tCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGcgcc                           <  2:2458937/100‑1 (MQ=255)
                                                                           cACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGcgccg                          <  2:486962/100‑1 (MQ=255)
                                                                               gTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGcgccagatc                      >  2:2844406/1‑95 (MQ=255)
                                                                               gTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCttt                      <  2:1534820/100‑1 (MQ=255)
                                                                               gTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCttt                      <  2:2564878/100‑1 (MQ=255)
                                                                                tccgACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGGCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTc                     <  2:189878/97‑1 (MQ=255)
                                                                                tCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTc                     <  2:2739476/100‑1 (MQ=255)
                                                                                 cTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCg                    >  2:3339937/1‑100 (MQ=255)
                                                                                  tGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGc                   >  2:3599295/1‑100 (MQ=255)
                                                                                  tGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGc                   >  1:2880998/1‑100 (MQ=255)
                                                                                  tGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGc                   <  2:850113/100‑1 (MQ=255)
                                                                                   ggccGGCGACGATATTTCAGCGTTTGCGCGTTTCAACCGCACAGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCt                  >  2:2894863/3‑100 (MQ=255)
                                                                                   gACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCt                  >  2:2359951/1‑100 (MQ=255)
                                                                                   gACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCt                  >  2:3697274/1‑100 (MQ=255)
                                                                                    aCCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTc                 >  2:2965988/1‑100 (MQ=255)
                                                                                     ccGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTcc                >  1:494315/1‑100 (MQ=255)
                                                                                     ccGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTcc                >  1:2129968/1‑100 (MQ=255)
                                                                                       ggCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAg              >  2:1849004/1‑100 (MQ=255)
                                                                                         cgacgaTTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGcc            <  2:1346325/100‑1 (MQ=255)
                                                                                             gaTATTGCAGCGTCGGCGCGGTTCAACCGCACCGTCGACCTTCGCCTGGCCTTCGGCAATCGCAATTTTCGCCTGCGCGCCGCTTTCGCTCCAg              <  1:3057452/94‑1 (MQ=255)
                                                                                               ttttTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAg      >  2:282469/1‑100 (MQ=255)
                                                                                               ttttGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAg      <  2:212868/100‑1 (MQ=255)
                                                                                               ttttGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAg      >  2:893588/1‑100 (MQ=255)
                                                                                               ttttGCAGCGATTGCGCGTTTCAACCGCAGCGTCGACTTTCACATGACCTTCGGCAATCCCGATTCACGCCGGCGAGCCGCTTTCGCTCCAGCCTTCCAg      <  1:125694/100‑1 (MQ=255)
                                                                                                  tGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGttt   >  2:2261995/1‑100 (MQ=255)
                                                                                                   gCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTc  <  2:2450487/100‑1 (MQ=255)
                                                                                                   gCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTc  <  1:330372/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTC  >  NC_000913/556622‑556820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑