BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,523,304	G→T	55.3%	P264H (CCT→CAT)	yjhG ←	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,523,304	0	G	T	55.3%	45.6 / 283.5	202	P264H (CCT→CAT)	yjhG	putative dehydratase
Reads supporting (aligned to +/- strand): ref base G (43/47); new base T (58/53); total (101/101)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 204 total aligned reads displayed.

CTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCC  >  NC_000913/4523206‑4523401

 tATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGc                                                                                                 <  2:2089210/100‑1 (MQ=255)

  aTCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCt                                                                                                >  2:2553531/1‑100 (MQ=255)

  aTCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGc                                                                                                 >  1:3247260/1‑99 (MQ=255)

  aTCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCt                                                                                                <  1:2455642/100‑1 (MQ=255)

      cTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCAcc                                                                                            <  1:772660/100‑1 (MQ=255)

       tttATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCg                                                                                           <  2:2338429/100‑1 (MQ=255)

       ccttccGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCg                                                                                           <  2:3545764/95‑1 (MQ=255)

        ttATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCgg                                                                                          >  2:1552021/1‑100 (MQ=255)

          aTCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGaa                                                                                        >  2:924682/1‑100 (MQ=255)

             ggTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGCAggg                                                                                     >  1:348750/1‑100 (MQ=255)

              gTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAgggg                                                                                    <  1:915757/100‑1 (MQ=255)

               tGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGc                                                                                   >  1:2147834/1‑100 (MQ=255)

                  gaATTTCCCGGGTGGTGATGCCTTTTTTTTTTTTTTTTAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAACGGGCCAg                                                                                >  1:883947/1‑100 (MQ=255)

                     tttCCCGGGTGGTGATGCCTTTTTTTTTTTTTTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGc                                                                             >  1:419370/1‑100 (MQ=255)

                     tttCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGc                                                                             >  1:2092196/1‑100 (MQ=255)

                      ttCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCt                                                                            <  1:3670002/100‑1 (MQ=255)

                       tCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTg                                                                           <  2:4268150/100‑1 (MQ=255)

                          cGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAAt                                                                        >  1:1624195/1‑100 (MQ=255)

                           gggTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGACGGGGCCAGGGCTGAAtg                                                                       >  1:989768/1‑100 (MQ=255)

                             gtggtgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAAtgtg                                                                     <  1:2417043/100‑1 (MQ=255)

                              tggtgATGCCTTTTTGGCTCAGGTTCACCGTAGTGCGCGCGGAAGCTCGGGCGATCTCCCTCCACACTGGGTCCCCGGAAGGAGCCAGGGCTGAAtttgn                                                                    <  1:536157/100‑2 (MQ=255)

                              tggtgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTgg                                                                    <  1:4214123/100‑1 (MQ=255)

                              tggtgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTgg                                                                    >  1:1544409/1‑100 (MQ=255)

                               ggtgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTggg                                                                   >  1:4135263/1‑100 (MQ=255)

                                gtgATGCCTTTTTGACTCAGGTGCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTgg                                                                    <  1:1724103/98‑1 (MQ=255)

                                 tgATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGAt                                                                 <  2:3022122/100‑1 (MQ=255)

                                  gATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGAtt                                                                >  1:1335698/1‑100 (MQ=255)

                                  gATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGAtt                                                                >  2:3331095/1‑100 (MQ=255)

                                    tGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGc                                                              >  2:392368/1‑100 (MQ=255)

                                     gCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGcc                                                             <  1:2032825/100‑1 (MQ=255)

                                       cTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAg                                                           >  2:430615/1‑100 (MQ=255)

                                       cTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAg                                                           <  1:2980026/100‑1 (MQ=255)

                                       cTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAg                                                           >  1:2344163/1‑100 (MQ=255)

                                         tttcGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTc                                                         <  1:1562822/100‑1 (MQ=255)

                                           ttGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTccc                                                       >  1:3161321/1‑100 (MQ=255)

                                            tGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCa                                                      <  1:672096/100‑1 (MQ=255)

                                             atcTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCaa                                                     <  1:2906616/98‑1 (MQ=255)

                                              aCTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAAt                                                    >  2:2589536/1‑100 (MQ=255)

                                              aCTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAAt                                                    <  2:1688044/100‑1 (MQ=255)

                                              aCTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAAt                                                    <  2:19276/100‑1 (MQ=255)

                                              aCTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGG‑CAGGGCTGAATGTGGGATTGCCAGTCCCAATc                                                   >  2:1383119/1‑100 (MQ=255)

                                               cTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAAt                                                    <  1:3056243/99‑1 (MQ=255)

                                               cTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATc                                                   >  2:2360121/1‑100 (MQ=255)

                                               cTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATc                                                   >  1:409483/1‑100 (MQ=255)

                                                 cAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCt                                                 >  2:673999/1‑100 (MQ=255)

                                                   ggTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTc                                               >  1:1832856/1‑100 (MQ=255)

                                                   ggTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTc                                               >  2:1611362/1‑100 (MQ=255)

                                                   ggTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTc                                               >  2:3836817/1‑100 (MQ=255)

                                                     ttCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCgg                                             <  1:4249678/100‑1 (MQ=255)

                                                     ttCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCgg                                             <  2:514296/100‑1 (MQ=255)

                                                     ttCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCgg                                             <  2:1293512/100‑1 (MQ=255)

                                                     ttCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCgg                                             >  1:643121/1‑100 (MQ=255)

                                                     ttCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCACCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCgg                                             <  2:3793215/100‑1 (MQ=255)

                                                       cAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACCGGCTCACCGGAAGGGGCCGCGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGcc                                           >  1:145309/1‑100 (MQ=255)

                                                        aGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGcca                                          <  1:11099/100‑1 (MQ=255)

                                                        aGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGcca                                          <  1:696471/100‑1 (MQ=255)

                                                            cAGCCCGCGCAGAAGCTCTGGCTATGTCCCCCCACCCATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGcc                                           <  1:2958687/95‑1 (MQ=255)

                                                             aGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCaccacc                                     >  2:4157018/1‑100 (MQ=255)

                                                              gCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCt                                    >  1:4056011/1‑100 (MQ=255)

                                                               cTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTg                                   >  1:3433075/1‑100 (MQ=255)

                                                               cTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTg                                   <  2:1502108/100‑1 (MQ=255)

                                                                 cgcgcgGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTgag                                 <  2:2169423/100‑1 (MQ=255)

                                                                 cgcgcgGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTgag                                 <  2:3805825/100‑1 (MQ=255)

                                                                 cgcgcgGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTgag                                 <  2:3634607/100‑1 (MQ=255)

                                                                 cgcgcgGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTgag                                 <  1:3860143/100‑1 (MQ=255)

                                                                  gcgcgGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTgaga                                <  1:1505951/100‑1 (MQ=255)

                                                                  gcgcgGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTgaga                                <  2:1229313/100‑1 (MQ=255)

                                                                   cgcgGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGAt                               >  2:3151934/1‑100 (MQ=255)

                                                                    gcgGAAGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATg                              <  2:376056/100‑1 (MQ=255)

                                                                         aGCTCTGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCg                         <  1:1264487/100‑1 (MQ=255)

                                                                           ctctGGCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGc                       >  1:1719120/1‑100 (MQ=255)

                                                                           ctctGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGc                       <  2:4022062/100‑1 (MQ=255)

                                                                           ctctGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGc                       >  2:1822309/1‑100 (MQ=255)

                                                                             ctGGCGCTCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGCCTCCATGTGCGCTTGCCCGTCCCCACCCTTCGCCCACCACCAgacctccccc                          >  1:3372093/1‑87 (MQ=255)

                                                                               ggCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTg                   >  2:1723227/1‑100 (MQ=255)

                                                                               ggCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTg                   >  2:15966/1‑100 (MQ=255)

                                                                               ggCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTg                   >  2:622713/1‑100 (MQ=255)

                                                                               ggCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTg                   <  1:2921212/100‑1 (MQ=255)

                                                                               ggCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTg                   <  2:4219384/100‑1 (MQ=255)

                                                                                gCGATCTCTCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGc                  <  1:1696010/100‑1 (MQ=255)

                                                                                gCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGc                  <  1:365091/100‑1 (MQ=255)

                                                                                gCGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGc                  >  2:278226/1‑100 (MQ=255)

                                                                                gCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGc                  <  1:3452497/100‑1 (MQ=255)

                                                                                 cGATCTCCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGcc                 >  1:3139699/1‑100 (MQ=255)

                                                                                 cGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGcc                 >  1:3734954/1‑100 (MQ=255)

                                                                                    tctcCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCaa              >  1:4020990/1‑100 (MQ=255)

                                                                                     ntcCCGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCaaa             >  2:1455102/2‑100 (MQ=255)

                                                                                       cccGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCaaa             >  1:960073/1‑98 (MQ=255)

                                                                                       cccGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCaaaaa           >  2:3852272/1‑100 (MQ=255)

                                                                                         cGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAAtt         >  1:2893189/1‑100 (MQ=255)

                                                                                         cGCCACACATGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAAtt         <  2:2647693/100‑1 (MQ=255)

                                                                                         cGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAAtt         <  2:1128821/100‑1 (MQ=255)

                                                                                          gCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTg        <  2:2915448/100‑1 (MQ=255)

                                                                                          gCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTg        <  2:1056397/100‑1 (MQ=255)

                                                                                            cacacaTGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGAc      >  2:681521/1‑100 (MQ=255)

                                                                                            cacacaTGCTCACCGGAAGGCGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTCCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGAc      <  1:3563087/100‑1 (MQ=255)

                                                                                             acacaTGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACa     <  1:2772422/100‑1 (MQ=255)

                                                                                              cacaTGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTCACAg    >  1:1015159/1‑100 (MQ=255)

                                                                                              cacaTGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTCAGATGTCCCGGCAGTGCACAAAAATTGActg    <  2:2880996/100‑3 (MQ=255)

                                                                                                  ggCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAgcc  >  1:462474/1‑98 (MQ=255)

CTATCGCTTTATCGGTGAGAATTTCCCGGGTGGTGATGCCTTTTTGACTCAGGTTCAGCGCAGCTCGCGCGGAAGCTCTGGCGATCTCCCGCCACACAGGCTCACCGGAAGGGGCCAGGGCTGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCC  >  NC_000913/4523206‑4523401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`