Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,627,706 T→G 46.4% F131C (TTT→TGT)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,627,7060TG46.4% 23.2 / 267.0 172F131C (TTT→TGT) nadRtrifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (53/39);  new base G (36/44);  total (89/83)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

TGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGT  >  NC_000913/4627607‑4627804
                                                                                                   |                                                                                                  
  ggTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTa                                                                                                  >  1:4252847/1‑100 (MQ=255)
  ggTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTa                                                                                                  <  1:3834299/100‑1 (MQ=255)
  ggTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTa                                                                                                  <  1:658692/100‑1 (MQ=255)
   gTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTTCGTTGGTTATTGCAAACTTGTaa                                                                                                 >  2:366503/1‑100 (MQ=255)
   gTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTaa                                                                                                 >  2:986089/1‑100 (MQ=255)
    ttttGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTaaa                                                                                                <  1:1700263/100‑1 (MQ=255)
      ttGACGGTACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAat                                                                                               >  1:2550869/1‑99 (MQ=255)
      ttGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAata                                                                                              <  1:3779082/100‑1 (MQ=255)
       tGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAatat                                                                                             <  1:157145/100‑1 (MQ=255)
        gACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAatat                                                                                             >  1:1557059/1‑99 (MQ=255)
        gACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATc                                                                                            <  1:1067624/100‑1 (MQ=255)
        gACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATc                                                                                            >  2:2150093/1‑100 (MQ=255)
          cGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAg                                                                                          >  2:1982012/1‑100 (MQ=255)
            aTACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGaa                                                                                        >  1:3111123/1‑100 (MQ=255)
            aTACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGaa                                                                                        >  2:1094862/1‑100 (MQ=255)
               cccGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGaaaaa                                                                                     >  1:3894538/1‑100 (MQ=255)
               cccGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGacaa                                                                                      >  1:3286131/1‑99 (MQ=255)
               cccGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGaaaaa                                                                                     <  2:3929314/100‑1 (MQ=255)
                 cGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAata                                                                                   >  2:73325/1‑100 (MQ=255)
                 cGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAata                                                                                   >  2:3105746/1‑100 (MQ=255)
                    gACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTc                                                                                >  1:2784882/1‑100 (MQ=255)
                     aCCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCATACTTTTAAATATCAGAAAAATATTCg                                                                               <  2:2599450/100‑1 (MQ=255)
                        gcgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCAt                                                                            >  2:1424077/1‑100 (MQ=255)
                           cgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCa                                                                         <  2:3327669/100‑1 (MQ=255)
                           cgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCa                                                                         <  2:4075067/100‑1 (MQ=255)
                            gttgttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCAt                                                                        >  2:422130/1‑100 (MQ=255)
                               gttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCt                                                                     >  1:4149616/1‑100 (MQ=255)
                                 tCGATGACAGTGCTATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCttt                                                                   <  1:2165870/100‑1 (MQ=255)
                                 tCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCTTTCATGCttt                                                                   >  1:3559747/1‑100 (MQ=255)
                                 tCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATAAGAAAAATATTCGCATTCATGCttt                                                                   >  1:2621878/1‑100 (MQ=255)
                                 tCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGCCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCttt                                                                   <  1:2725658/100‑1 (MQ=255)
                                  cGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTc                                                                  >  2:1764842/1‑100 (MQ=255)
                                   gAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCa                                                                 >  1:188453/1‑100 (MQ=255)
                                   gAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCa                                                                 <  2:3580331/100‑1 (MQ=255)
                                   gAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCa                                                                 <  2:695329/100‑1 (MQ=255)
                                   gAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCa                                                                 <  2:3915396/100‑1 (MQ=255)
                                    aaGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCaa                                                                >  1:2457376/1‑100 (MQ=255)
                                    aaGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCaa                                                                <  2:1426709/100‑1 (MQ=255)
                                     aGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAAc                                                               <  1:705421/100‑1 (MQ=255)
                                      gacggggcgaTGTCGCAGCAGCCGCCCTTGCAGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATAAGAAAAATATTCGCATTCATGCTTTCAAc                                                               <  1:1052371/90‑1 (MQ=255)
                                      gACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACg                                                              <  2:3326613/100‑1 (MQ=255)
                                        cAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGaa                                                            >  2:2505427/1‑100 (MQ=255)
                                         aGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAag                                                           <  2:210437/100‑1 (MQ=255)
                                            gCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAgg                                                        >  1:753325/1‑100 (MQ=255)
                                             ccATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAggg                                                       >  2:3705775/1‑100 (MQ=255)
                                               aTGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCa                                                     <  2:3755009/100‑1 (MQ=255)
                                                 gTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATg                                                   >  2:4217991/1‑100 (MQ=255)
                                                  tCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATgg                                                  <  1:1244202/100‑1 (MQ=255)
                                                   cGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGa                                                 >  2:3172877/1‑100 (MQ=255)
                                                   cGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGa                                                 >  2:102156/1‑100 (MQ=255)
                                                      agcagcCGACCGTGCCAGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGcc                                              <  1:2447689/100‑1 (MQ=255)
                                                       gcagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATTCTTTCAACGAAGAGGGCATGGAGCCg                                             >  2:1560859/1‑100 (MQ=255)
                                                       gcagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCg                                             >  2:189608/1‑100 (MQ=255)
                                                       gcagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCg                                             <  2:3406571/100‑1 (MQ=255)
                                                       gcagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCg                                             >  1:3406559/1‑100 (MQ=255)
                                                       gcagcCGACCGTGCCGGATCGTATGCGTTGGATATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCg                                             <  1:2885367/100‑1 (MQ=255)
                                                        cagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAg                                                >  1:947208/1‑96 (MQ=255)
                                                        cagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGt                                            <  2:3852409/100‑1 (MQ=255)
                                                         agcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTa                                           <  2:3327113/100‑1 (MQ=255)
                                                           ccGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATc                                         >  1:1788274/1‑100 (MQ=255)
                                                             gACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCg                                       >  2:2732609/1‑100 (MQ=255)
                                                             gACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGt                                            >  1:1827365/1‑95 (MQ=255)
                                                              aCCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGc                                      <  1:1522037/100‑1 (MQ=255)
                                                              aCCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGc                                      >  1:2219506/1‑100 (MQ=255)
                                                                 gTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACg                                   >  2:19515/1‑100 (MQ=255)
                                                                  tGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACgg                                  <  2:3844154/100‑1 (MQ=255)
                                                                    ccGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCt                                >  2:13620/1‑100 (MQ=255)
                                                                    ccGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCt                                >  1:3167834/1‑100 (MQ=255)
                                                                    ccGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCt                                <  2:3062054/100‑1 (MQ=255)
                                                                         tCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAt                           >  1:3492766/1‑100 (MQ=255)
                                                                          cGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGTCTGGGAtg                          >  2:2765107/1‑100 (MQ=255)
                                                                          cGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtg                          <  2:4151683/100‑1 (MQ=255)
                                                                          cGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCAGGGAtg                          <  2:2586668/100‑1 (MQ=255)
                                                                          cGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtg                          <  2:1707118/100‑1 (MQ=255)
                                                                          cGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtg                          <  1:2764820/100‑1 (MQ=255)
                                                                           gTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGTAGCCGTATCCGCACGCCAGGGAtgt                         >  1:1268916/1‑100 (MQ=255)
                                                                           gTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCAGGGAtgt                         >  1:3216973/1‑100 (MQ=255)
                                                                            tCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCAGGGAtgtg                        <  2:3822260/100‑1 (MQ=255)
                                                                             cTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtgtgt                       >  2:2329817/1‑100 (MQ=255)
                                                                              tGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtgtgtg                      <  2:801714/100‑1 (MQ=255)
                                                                              tGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCAGGGAtgtgt                       >  1:4063522/1‑99 (MQ=255)
                                                                               gCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTgg                     <  1:1762220/100‑1 (MQ=255)
                                                                               gCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCACACGGCTGGGATGTGTgg                     >  2:875054/1‑100 (MQ=255)
                                                                                 gTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAg                   <  1:3646321/100‑1 (MQ=255)
                                                                                 gTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAg                   >  2:1092342/1‑100 (MQ=255)
                                                                                  ttGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGc                  <  2:1346558/100‑1 (MQ=255)
                                                                                     gTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAAc               >  2:486474/1‑100 (MQ=255)
                                                                                      ttATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACg              <  2:3272886/100‑1 (MQ=255)
                                                                                       tATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACgg             <  2:1190967/100‑1 (MQ=255)
                                                                                       tATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACgg             <  1:3600792/100‑1 (MQ=255)
                                                                                        aTTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGc            <  2:2039625/100‑1 (MQ=255)
                                                                                        aTTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCAGGGATGTGTGGAGCAACGGc            <  1:2092688/100‑1 (MQ=255)
                                                                                        aTTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGc            >  2:1658167/1‑100 (MQ=255)
                                                                                         ttGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCAGGGATGTGTGGAGCAACGGCa           >  2:1583101/1‑100 (MQ=255)
                                                                                         ttGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCa           <  1:3095408/100‑1 (MQ=255)
                                                                                          tGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCAGGGATGTGTGGAGCAACGGCAt          >  2:3474501/1‑100 (MQ=255)
                                                                                                cTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCaaaa     <  1:2410754/99‑1 (MQ=255)
                                                                                                cTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCAGGGATGTGTGGAGCAACGGCATCaaaaa    >  2:3887639/1‑100 (MQ=255)
                                                                                                cTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCaaaaa    >  2:61830/1‑100 (MQ=255)
                                                                                                 ttttAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAg   >  2:1944088/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGT  >  NC_000913/4627607‑4627804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: