BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,631,700	T→C	100%	L323P (CTT→CCT)	slt →	lytic murein transglycosylase, soluble

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,700	0	T	C	100.0%	709.2 / ‑6.7	200	L323P (CTT→CCT)	slt	lytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (102/97); minor base G (0/1); total (102/98)

Only 100 of 200 total aligned reads displayed.

GGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTT  >  NC_000913/4631603‑4631796

ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTg                                                                                                >  1:1507919/1‑100 (MQ=255)

ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTg                                                                                                <  1:2975301/100‑1 (MQ=255)

       aTGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCgg                                                                                         >  2:1268571/1‑100 (MQ=255)

        tGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGc                                                                                        <  1:1662985/100‑1 (MQ=255)

        tGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGc                                                                                        <  2:3734215/100‑1 (MQ=255)

         gTCACCGACGAGCAGGCGAAATGGCGCGATGACGTCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCg                                                                                       <  1:2253705/100‑1 (MQ=255)

         gTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCg                                                                                       >  1:3634936/1‑100 (MQ=255)

           cACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAt                                                                                     >  2:408567/1‑100 (MQ=255)

             ccGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                   >  1:594603/1‑100 (MQ=255)

             ccGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                   >  1:142677/1‑100 (MQ=255)

             ccGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                   >  2:3189143/1‑100 (MQ=255)

               gacgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcgtc                                                                                 >  2:1000766/1‑100 (MQ=255)

                acgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcgtcg                                                                                <  2:3904800/100‑1 (MQ=255)

                  gaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTCATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTcgcg                                                                              <  2:798061/100‑1 (MQ=255)

                   aGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCgg                                                                             <  2:3303152/100‑1 (MQ=255)

                    gCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGc                                                                            >  2:4194349/1‑100 (MQ=255)

                    gCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGc                                                                            <  2:2503480/100‑1 (MQ=255)

                    gCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGc                                                                            >  1:3075434/1‑100 (MQ=255)

                       ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGTGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                         >  2:3922749/1‑100 (MQ=255)

                       ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                         >  1:3961723/1‑100 (MQ=255)

                       ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                         >  2:181692/1‑100 (MQ=255)

                       ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                         >  2:2073807/1‑100 (MQ=255)

                       ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                         >  1:74271/1‑100 (MQ=255)

                         cGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGaa                                                                       >  2:2612169/1‑100 (MQ=255)

                          gAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAAt                                                                      >  1:3711876/1‑100 (MQ=255)

                          gAAAGGGCGCGATGACGCCATGATGCGCTCGCAATCTACGTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAAt                                                                      <  2:3602985/100‑1 (MQ=255)

                           aaaTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATa                                                                     <  2:576674/100‑1 (MQ=255)

                           aaaTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATa                                                                     <  2:3477814/100‑1 (MQ=255)

                            aaTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAc                                                                    <  2:1628939/100‑1 (MQ=255)

                            aaTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAc                                                                    >  2:266166/1‑100 (MQ=255)

                             aTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGCATGGCGCCTGGCACCGGCGATCGTCGCGGGCTGGATAc                                                                    >  1:2164786/1‑99 (MQ=255)

                               ggCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                                 <  1:108071/100‑1 (MQ=255)

                                  gcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggct                                                              <  1:4244780/100‑1 (MQ=255)

                                   cgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggctg                                                             <  2:4044157/100‑1 (MQ=255)

                                   cgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggctg                                                             >  1:2307316/1‑100 (MQ=255)

                                    gATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCGTGGCACCGGCGATCGTCGCGGCCTCAATACctggctgg                                                            <  1:2635857/100‑1 (MQ=255)

                                    gATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTTGCACCGGCGATCGTCGCGGCCTGAATACctggctgg                                                            <  2:3017473/100‑1 (MQ=255)

                                    gATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggctgg                                                            >  2:2321142/1‑100 (MQ=255)

                                    gATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGCCGCGGCCTGAATACctggctgg                                                            >  1:23492/1‑100 (MQ=255)

                                      tGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcg                                                          >  2:3166995/1‑100 (MQ=255)

                                      tGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcg                                                          <  2:967288/100‑1 (MQ=255)

                                       gACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcgc                                                         >  2:759868/1‑100 (MQ=255)

                                        aCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGcgcg                                                        >  1:3753708/1‑100 (MQ=255)

                                         cGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGt                                                       >  1:845773/1‑100 (MQ=255)

                                          gCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTc                                                      <  1:556749/100‑1 (MQ=255)

                                           ncATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGGCt                                                     >  2:1045997/2‑100 (MQ=255)

                                           ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCt                                                     <  2:948713/100‑1 (MQ=255)

                                           ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCt                                                     <  1:2512599/100‑1 (MQ=255)

                                            cATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTg                                                    <  1:1354760/100‑1 (MQ=255)

                                              ttATGCGCTCGCAATCTACTTCGCTTCTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCACGGCCTGAATACCTGGCTGGCGCGTCTGcc                                                  <  1:191801/100‑1 (MQ=255)

                                              ttATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGcc                                                  <  1:2722790/100‑1 (MQ=255)

                                                aTGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTa                                                <  1:941384/100‑1 (MQ=255)

                                                 tGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTAt                                               >  2:1997700/1‑100 (MQ=255)

                                                  gcgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                              <  2:277297/100‑1 (MQ=255)

                                                   cgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATgg                                             >  1:442655/1‑100 (MQ=255)

                                                   cgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATgg                                             <  1:3200183/100‑1 (MQ=255)

                                                   cgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATgg                                             >  2:424449/1‑100 (MQ=255)

                                                        gCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCg                                        <  2:719782/100‑1 (MQ=255)

                                                         cAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCga                                       <  2:2274567/100‑1 (MQ=255)

                                                          aaTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaa                                      >  1:2621618/1‑100 (MQ=255)

                                                          aaTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaa                                      >  2:717375/1‑100 (MQ=255)

                                                           aTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaa                                     <  2:3075446/100‑1 (MQ=255)

                                                           aTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaa                                     >  1:3334728/1‑100 (MQ=255)

                                                           aTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaa                                     >  1:3226724/1‑100 (MQ=255)

                                                           aTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAACTGGCTGGCGCGTCTGCCTATGGAAGCgaaa                                     >  2:1149508/1‑100 (MQ=255)

                                                             cTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaaga                                   >  2:2347751/1‑100 (MQ=255)

                                                               aCTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagaga                                 >  2:1385637/1‑100 (MQ=255)

                                                                cTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaa                                >  2:1669797/1‑100 (MQ=255)

                                                                cTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaa                                >  1:1800808/1‑100 (MQ=255)

                                                                cTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaa                                <  1:3479026/100‑1 (MQ=255)

                                                                 ttCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaa                               >  1:1164095/1‑100 (MQ=255)

                                                                    ncTTATTGAACGCCGTGTACGAATGGCGCCTGGGACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGAGTCTGCCTATGGAAGCGAAAGAGAAAGAt                            >  1:2563735/2‑100 (MQ=255)

                                                                    gCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGAt                            >  2:4163787/1‑100 (MQ=255)

                                                                     cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                           <  2:4139946/100‑1 (MQ=255)

                                                                     cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                           <  1:1000763/100‑1 (MQ=255)

                                                                     cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                           <  2:2289969/100‑1 (MQ=255)

                                                                      ttATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGa                          >  2:1219969/1‑100 (MQ=255)

                                                                       tATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGaa                         >  1:343984/1‑100 (MQ=255)

                                                                         ttGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATg                       <  2:3527555/100‑1 (MQ=255)

                                                                          tGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATgg                      >  2:3969973/1‑100 (MQ=255)

                                                                          ccgatctcGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATgg                      <  1:2882068/93‑1 (MQ=255)

                                                                           gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGc                     <  2:3401509/100‑1 (MQ=255)

                                                                           gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGc                     >  2:1479285/1‑100 (MQ=255)

                                                                           gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGc                     >  2:1882236/1‑100 (MQ=255)

                                                                             aCGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCTTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt                   <  1:2742983/100‑1 (MQ=255)

                                                                             aCGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt                   <  1:4023805/100‑1 (MQ=255)

                                                                                ccGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTAc                >  2:3250469/1‑100 (MQ=255)

                                                                                  gtgtACGAATGGCGCCTGTCAGAGGCGATCGTCGCGGTTAGAATAGCTGGCTGGCGCGTCGGCGTATGGTAGCGAAAGAGAAAGATAAATGGCGTTAc                >  1:2927428/1‑98 (MQ=255)

                                                                                   tgtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTgg             >  1:3331010/1‑100 (MQ=255)

                                                                                   tgtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTgg             >  2:1600075/1‑100 (MQ=255)

                                                                                   tgtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTgg             >  1:781535/1‑100 (MQ=255)

                                                                                    gtACGAATGGCGCCTGTCACCGGCGATCGTCGCGGCCTGAATACCTGGCGGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGc            <  1:4213261/100‑1 (MQ=255)

                                                                                    gtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTggn            <  1:1045994/100‑2 (MQ=255)

                                                                                    gtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGc            <  2:1689680/100‑1 (MQ=255)

                                                                                     tACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCa           <  1:1546793/100‑1 (MQ=255)

                                                                                       cGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAgg         >  2:3854925/1‑100 (MQ=255)

                                                                                       cGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAgg         >  2:1349963/1‑100 (MQ=255)

                                                                                        gAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGc        >  2:624938/1‑100 (MQ=255)

                                                                                           tGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGa     <  1:2037132/100‑1 (MQ=255)

                                                                                           tGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGa     <  2:440387/100‑1 (MQ=255)

GGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTT  >  NC_000913/4631603‑4631796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`