Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 568,691 T→A 15.4% intergenic (+44/‑211) emrE → / → ybcK DLP12 prophage; multidrug resistance protein/DLP12 prophage; putative recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913568,6910TA15.4% 430.5 / 63.8 162intergenic (+44/‑211)emrE/ybcKDLP12 prophage; multidrug resistance protein/DLP12 prophage; putative recombinase
Reads supporting (aligned to +/- strand):  ref base T (69/68);  new base A (15/10);  total (84/78)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

Only 100 of 185 total aligned reads displayed.

TGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATT  >  NC_000913/568592‑568789
                                                                                                   |                                                                                                  
tGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatatt                                                                                                    <  2:2700074/100‑1 (MQ=255)
 gATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatattt                                                                                                   >  2:624174/1‑100 (MQ=255)
 gATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatattt                                                                                                   >  2:1588447/1‑100 (MQ=255)
  aTTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGCAGGCTCttatattta                                                                                                  >  2:4205735/1‑100 (MQ=255)
     tgtgCTGATGTGTTGATCATTAACTTATCGTCACGAAGCACACCACATTAACATTATTTGTTTCTCATCGACGAAACTATGGAGGCTCttatatttatat                                                                                               <  1:2830739/100‑1 (MQ=255)
      gtgCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATg                                                                                              >  2:369159/1‑100 (MQ=255)
      gtgCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATg                                                                                              <  1:2572357/100‑1 (MQ=255)
        gCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAg                                                                                            <  2:1741296/100‑1 (MQ=255)
         ccGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGc                                                                                           >  1:744667/1‑100 (MQ=255)
          cGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGcc                                                                                          <  2:3685871/100‑1 (MQ=255)
          cGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGcc                                                                                          <  1:2525637/100‑1 (MQ=255)
           ggTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCt                                                                                         >  1:2553394/1‑100 (MQ=255)
            gtgtgtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCTc                                                                                        <  2:3398739/100‑1 (MQ=38)
            gtgtgtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCTc                                                                                        <  2:1921699/100‑1 (MQ=38)
             tgtgtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCTcc                                                                                       <  1:3884728/100‑2 (MQ=38)
               tgtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGtt                                                                                     >  1:4206728/1‑100 (MQ=255)
                gtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGttt                                                                                    <  2:1169923/100‑1 (MQ=255)
                gtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGttt                                                                                    <  1:2696143/100‑1 (MQ=255)
                 ttGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGtttt                                                                                   <  1:704877/100‑1 (MQ=255)
                  tGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTa                                                                                  >  1:233681/1‑100 (MQ=255)
                  tGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTa                                                                                  <  2:1036163/100‑1 (MQ=255)
                  tGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTa                                                                                  <  2:1126058/100‑1 (MQ=255)
                  tGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTa                                                                                  >  2:788850/1‑100 (MQ=255)
                  tGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTa                                                                                  >  2:1973490/1‑100 (MQ=255)
                  ngATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTa                                                                                  >  1:3248425/2‑100 (MQ=255)
                   gATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTAt                                                                                 <  2:1857260/100‑1 (MQ=255)
                     ttattaATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGc                                                                               >  1:1760802/1‑100 (MQ=255)
                      tattaATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCt                                                                              >  1:3401260/1‑100 (MQ=255)
                        ttaATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCttt                                                                            >  2:2155789/1‑100 (MQ=255)
                        ttaATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCttt                                                                            >  1:1966351/1‑100 (MQ=255)
                         taATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCtttt                                                                           <  1:116791/100‑1 (MQ=255)
                          aaTTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCttttt                                                                          <  1:1217662/100‑1 (MQ=255)
                            tttATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGt                                                                        <  2:2827651/100‑1 (MQ=255)
                             ttATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGtt                                                                       <  1:2746453/100‑1 (MQ=255)
                              tATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTa                                                                      >  1:1935508/1‑100 (MQ=255)
                              tATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTa                                                                      >  1:876027/1‑100 (MQ=255)
                                ttGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAAt                                                                    <  2:2519109/100‑1 (MQ=255)
                                 tGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATg                                                                   <  1:3111587/100‑1 (MQ=255)
                                  gTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGt                                                                  >  2:3137844/1‑100 (MQ=255)
                                   tCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTc                                                                 <  2:4052774/100‑1 (MQ=255)
                                    cACGAAGCACACCACATTAAAATAATTTGTATCGAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTAGTTAATGTCt                                                                >  1:2810688/1‑100 (MQ=255)
                                      cGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCttt                                                              >  2:1086739/1‑100 (MQ=255)
                                           cacaCCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAtttt                                                         <  2:2833842/100‑1 (MQ=255)
                                            acacCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAttttt                                                        >  2:206888/1‑100 (MQ=255)
                                             caccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAtttttt                                                       >  1:2716618/1‑100 (MQ=255)
                                             caccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAtttttt                                                       <  2:4140279/100‑1 (MQ=255)
                                             caccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAtttttt                                                       <  1:4233060/100‑1 (MQ=255)
                                              accacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTa                                                      <  2:515414/100‑1 (MQ=255)
                                                  caTTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTa                                                  >  1:3531296/1‑100 (MQ=255)
                                                   aTTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTAt                                                 >  2:2138908/1‑100 (MQ=255)
                                                   aTTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTAt                                                 >  2:3552545/1‑100 (MQ=255)
                                                     tAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTc                                               >  1:1036510/1‑100 (MQ=255)
                                                       aaaTAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCtt                                             >  1:1639106/1‑100 (MQ=255)
                                                        aataatTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCttt                                            <  1:1574313/100‑1 (MQ=255)
                                                          taatTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtg                                          <  2:3185439/100‑1 (MQ=255)
                                                          taatTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtg                                          <  1:3915159/100‑1 (MQ=255)
                                                            atTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtgtg                                        >  2:444640/1‑100 (MQ=255)
                                                            atTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtgtg                                        >  2:3932528/1‑100 (MQ=255)
                                                                gTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCttt                                    >  2:81391/1‑100 (MQ=255)
                                                                 tttCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTc                                   <  1:1438605/100‑1 (MQ=255)
                                                                  ttCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTc                                   >  2:388217/1‑99 (MQ=255)
                                                                  ttCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCa                                  <  1:215036/100‑1 (MQ=255)
                                                                  ttCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCa                                  <  1:640858/100‑1 (MQ=255)
                                                                    cTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAg                                <  2:1293274/100‑1 (MQ=255)
                                                                      aaaCGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGAt                              >  2:875153/1‑100 (MQ=255)
                                                                      aaaCGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGAt                              >  1:4163749/1‑100 (MQ=255)
                                                                        aCGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTa                            <  2:2459746/100‑1 (MQ=255)
                                                                        aCGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTa                            <  2:2747176/100‑1 (MQ=255)
                                                                         cGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTAt                           >  1:470891/1‑100 (MQ=255)
                                                                           aCTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATgg                         <  1:4126472/100‑1 (MQ=255)
                                                                            cTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGc                        >  2:1003279/1‑100 (MQ=255)
                                                                             gcataaaacgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAATATTATGTCg                       >  1:2051994/10‑100 (MQ=21)
                                                                              cataaaacgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGt                      >  1:4094025/9‑100 (MQ=25)
                                                                              aaaaTATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTATTTTGTGCTTTCAAGATTATGGCGt                      >  2:535006/1‑100 (MQ=255)
                                                                               ataaaacgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTa                     >  2:2935011/8‑100 (MQ=25)
                                                                                taaaacgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTaa                    >  2:4013516/7‑100 (MQ=37)
                                                                                taaaacgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTaa                    >  2:4013217/7‑100 (MQ=37)
                                                                                  tatGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGa                  >  1:2981964/1‑100 (MQ=255)
                                                                                  aaacgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGa                  >  2:3273829/5‑100 (MQ=37)
                                                                                   atGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGaa                 >  1:2933029/1‑100 (MQ=255)
                                                                                    tGGAGGATCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATATATTATATTGTGCTTTCAAGATTATGGCGTAAGa                  <  1:1717175/98‑1 (MQ=255)
                                                                                    acgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTTCAGATTATGGCGTTAGa                  >  1:1011939/3‑98 (MQ=37)
                                                                                     ggAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGaaaa               >  1:3028615/1‑100 (MQ=255)
                                                                                     ggAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGaaaa               <  1:2911069/100‑1 (MQ=255)
                                                                                      gAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAAt              <  2:3604569/100‑1 (MQ=255)
                                                                                        ggCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTg            >  2:3802936/1‑100 (MQ=255)
                                                                                         gCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGc           <  1:1542986/100‑1 (MQ=255)
                                                                                          ctctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAATATTATGGCGTAAGAAAATTGCa          >  2:2985500/1‑100 (MQ=255)
                                                                                          ctctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCa          >  1:3591786/1‑100 (MQ=255)
                                                                                           tctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGc           <  1:4139459/98‑1 (MQ=255)
                                                                                           tctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCaa         <  2:2536969/100‑1 (MQ=255)
                                                                                            ctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAAt        >  2:761190/1‑100 (MQ=255)
                                                                                             ttatatttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATa       >  1:218825/1‑100 (MQ=255)
                                                                                              tatatttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGTGTAAGAAAATTGCAATAc      >  1:3286258/1‑100 (MQ=255)
                                                                                                tatttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGa    >  2:3760917/1‑100 (MQ=255)
                                                                                                tatttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGa    >  1:2773254/1‑100 (MQ=255)
                                                                                                tatttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGa    <  2:4163771/100‑1 (MQ=255)
                                                                                                tatttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGa    >  1:3136272/1‑100 (MQ=255)
                                                                                                tatttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGa    >  2:1561809/1‑100 (MQ=255)
                                                                                                 atttatatGAGCCTCGTTTTATGCTTTTTTTTAATGTATTTATTTTTTATGTATTCTTTTGTGCTTTCAATATTATGGCGTAAGAAAATTGCAATACGat   >  1:1420529/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATT  >  NC_000913/568592‑568789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: