Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 171,343 G→A 38.2% A257T (GCC→ACC)  fhuD → iron(3+)‑hydroxamate import ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913171,3430GA38.2% 175.3 / 201.6 204A257T (GCC→ACC) fhuDiron(3+)‑hydroxamate import ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (67/59);  new base A (45/33);  total (112/92)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 209 total aligned reads displayed.

ATCGATCGTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCTGGAT  >  NC_000913/171244‑171441
                                                                                                   |                                                                                                  
atcgatcgTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAgg                                                                                                    <  1:4183603/100‑1 (MQ=255)
  cgatcgTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGcc                                                                                                  <  2:1446162/100‑1 (MQ=255)
   gatcgTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCa                                                                                                 >  1:2600504/1‑100 (MQ=255)
   gatcgTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCa                                                                                                 >  2:3043880/1‑100 (MQ=255)
       gTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGcc                                                                                             >  1:1591829/1‑100 (MQ=255)
       gTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGcc                                                                                             <  1:3659436/100‑1 (MQ=255)
        tCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTGTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCg                                                                                            >  2:1589798/1‑100 (MQ=255)
         cTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGt                                                                                           >  2:1902873/1‑100 (MQ=255)
          tGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGtt                                                                                          <  2:2961730/100‑1 (MQ=255)
          tGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGtt                                                                                          <  1:3608433/100‑1 (MQ=255)
           ggcggcGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGttt                                                                                         >  2:31964/1‑100 (MQ=255)
           ggcggcGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGttt                                                                                         >  2:2778869/1‑100 (MQ=255)
            gcggcgTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTg                                                                                        <  1:3896973/100‑1 (MQ=255)
            gcggcgTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTg                                                                                        <  1:1861785/100‑1 (MQ=255)
            gcggcgTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTg                                                                                        <  2:1873526/100‑1 (MQ=255)
            gcggcgTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTg                                                                                        <  2:1319397/100‑1 (MQ=255)
                 gTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCcgc                                                                                   >  1:2720610‑M2/2‑100 (MQ=255)
                  tataAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCcgcg                                                                                  <  2:645852/100‑1 (MQ=255)
                    taAAGACGTTGCTGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGcc                                                                                >  2:805619/1‑100 (MQ=255)
                    taAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGcc                                                                                >  2:823407/1‑100 (MQ=255)
                    taAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGcc                                                                                >  1:1424980/1‑100 (MQ=255)
                    taAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGcc                                                                                >  1:2775678/1‑100 (MQ=255)
                     aaaGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCg                                                                               >  2:3664016/1‑100 (MQ=255)
                     aaaGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCg                                                                               >  1:127048/1‑100 (MQ=255)
                       aGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGa                                                                             >  2:742773/1‑100 (MQ=255)
                         aCGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACg                                                                           <  2:2147902/100‑1 (MQ=255)
                         aCGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACg                                                                           <  1:3156010/100‑1 (MQ=255)
                          cGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGc                                                                          >  2:25038/1‑100 (MQ=255)
                          cGTTGATGTGCTCTGTTTTAAACAAGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAAGCCATGCCGTTTGTCCGCGCCGGACGc                                                                          >  1:596694/1‑100 (MQ=255)
                           gTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCt                                                                         >  1:2204833/1‑100 (MQ=255)
                           gTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCt                                                                         >  1:4058241/1‑100 (MQ=255)
                              gATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTc                                                                      <  1:1793632/100‑1 (MQ=255)
                              gATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTc                                                                      <  1:1447660/100‑1 (MQ=255)
                              gATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTc                                                                      <  1:121712/100‑1 (MQ=255)
                              gATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTc                                                                      <  2:3572884/100‑1 (MQ=255)
                              gATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTc                                                                      <  2:398670/100‑1 (MQ=255)
                               aTGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCGGTTTGTCCGCGCCGGACGCTTTc                                                                      >  1:2032318/1‑99 (MQ=255)
                                tgtgCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAg                                                                    <  2:3229238/100‑1 (MQ=255)
                                 gtgCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAgc                                                                   >  1:2401820/1‑100 (MQ=255)
                                 gtgCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCCGACGCTTTCAgc                                                                   <  2:2802889/100‑1 (MQ=255)
                                  tgCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAgcg                                                                  >  2:2273190/1‑100 (MQ=255)
                                   gCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCTTTTGTCCGCGCCGGACGCTTTCAgc                                                                   >  1:3176327/1‑98 (MQ=255)
                                   gCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAgcgc                                                                 <  1:3769602/100‑1 (MQ=255)
                                    ctctGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGcgcg                                                                <  2:5999/100‑1 (MQ=255)
                                    ctctGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGcgcg                                                                <  2:2459008/100‑1 (MQ=255)
                                     tctGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGt                                                               <  2:3755968/100‑1 (MQ=255)
                                          tttGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTg                                                          <  2:1245694/100‑1 (MQ=255)
                                           ttGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGc                                                         <  2:3954500/100‑1 (MQ=255)
                                            tGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCTATGCCGTTTGTCCGCGCCGGACGCTTTCTGCGCGTACCTgta                                                        <  2:2880718/100‑3 (MQ=255)
                                             gATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAg                                                       >  2:1837763/1‑100 (MQ=255)
                                             gATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAg                                                       >  1:36634/1‑100 (MQ=255)
                                              aTCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGt                                                      >  2:1146310/1‑100 (MQ=255)
                                              aTCACGACAACAGCAAAGACATAGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCa                                                        <  2:508520/98‑1 (MQ=255)
                                               tCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTc                                                     >  2:1227341/1‑100 (MQ=255)
                                                cACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCt                                                    >  2:3639680/1‑100 (MQ=255)
                                                 aCGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTg                                                   >  1:1181055/1‑100 (MQ=255)
                                                  cGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTgg                                                  >  2:1555291/1‑100 (MQ=255)
                                                  cGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTgg                                                  <  1:858902/100‑1 (MQ=255)
                                                   gACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGt                                                 <  2:1192473/100‑1 (MQ=255)
                                                     caacaGCCAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCTCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGttt                                               <  1:2293467/100‑1 (MQ=255)
                                                       acaGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTa                                             >  2:1797939/1‑100 (MQ=255)
                                                       acaGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTa                                             <  2:3874023/100‑1 (MQ=255)
                                                        caGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTAt                                            <  1:345141/100‑1 (MQ=255)
                                                        caGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTAt                                            <  1:615758/100‑1 (MQ=255)
                                                          gCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATgg                                          >  1:1184072/1‑100 (MQ=255)
                                                          gCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATgg                                          >  2:1970102/1‑100 (MQ=255)
                                                          gCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATgg                                          >  1:1685149/1‑100 (MQ=255)
                                                             aaGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGc                                       <  2:3335063/100‑1 (MQ=255)
                                                              aGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCg                                      >  2:2328760/1‑100 (MQ=255)
                                                                   tGGATGCGCTAATGGCAACGCCGCAGTGGCAGGCCATGCCGTTTGTCAGCGCCGGACGCTTTCAGCGCGTAACTACAGTCTGGTTTTATGGTGCGACGct                                 >  1:725065/1‑100 (MQ=255)
                                                                    ggATGCGCT‑ATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGctct                               >  1:3045688/1‑100 (MQ=255)
                                                                       tGCGCTAATGGCAACGCCGCTGTGGTAGGTCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTTGTTTTATGGTGCGCCGCTCTCg                             <  1:998334/100‑1 (MQ=255)
                                                                         cgcTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGc                           <  2:3521946/100‑1 (MQ=255)
                                                                         cgcTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGc                           >  2:2825960/1‑100 (MQ=255)
                                                                          gcTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCa                          >  2:2949272/1‑100 (MQ=255)
                                                                            tAATGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAAt                        <  2:3411803/100‑1 (MQ=255)
                                                                             aaTGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATg                       <  2:4250750/100‑1 (MQ=255)
                                                                             aaTGGCAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATg                       >  2:616757/1‑100 (MQ=255)
                                                                                ggCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCAc                    >  1:1370218/1‑100 (MQ=255)
                                                                                ggCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCAc                    <  1:961056/100‑1 (MQ=255)
                                                                                  cAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACtt                  >  2:2359811/1‑100 (MQ=255)
                                                                                  cAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACtt                  >  1:1024654/1‑100 (MQ=255)
                                                                                  cAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACtt                  >  2:3935070/1‑100 (MQ=255)
                                                                                  cAACGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCCGGACTTATGGTGCGACTCTCTCGGCAATGCACtt                  >  2:3426656/1‑100 (MQ=255)
                                                                                   aaCGCCGCTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACttt                 >  2:2249935/1‑100 (MQ=255)
                                                                                     cgccgcTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTCCCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTtgt               >  2:961357/1‑100 (MQ=255)
                                                                                     cgccgcTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTtgt               >  2:2061823/1‑100 (MQ=255)
                                                                                     cgccgcTGTGGCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTtgt               >  1:2181381/1‑100 (MQ=255)
                                                                                          cTGGGGCAGGCCATGCCGTGTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGcgcg          <  1:2645001/100‑1 (MQ=255)
                                                                                             tgGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTc       <  2:4072351/100‑1 (MQ=255)
                                                                                              ggCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCt      >  1:4032116/1‑100 (MQ=255)
                                                                                              ggCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGAACTTTGTGCGCGTTCt      >  1:2497758/1‑100 (MQ=255)
                                                                                               gCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCTg     <  2:4032136/100‑1 (MQ=255)
                                                                                               gCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCTg     <  1:934805/100‑1 (MQ=255)
                                                                                               gCAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCTg     <  1:3285197/100‑1 (MQ=255)
                                                                                                cAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCtc     <  1:3556144/99‑2 (MQ=255)
                                                                                                cAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCTgg    <  1:2061819/100‑1 (MQ=255)
                                                                                                cAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCTgg    <  2:2592203/100‑1 (MQ=255)
                                                                                                cAGACCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCTgg    >  1:2839311/1‑100 (MQ=255)
                                                                                                  ggCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCTGGAt  >  2:1492836/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
ATCGATCGTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCACGACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTTGTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCGGCAATGCACTTTGTGCGCGTTCTGGAT  >  NC_000913/171244‑171441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑