Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 569,161 A→C 16.4% D87A (GAC→GCC) ‡ ybcK → DLP12 prophage; putative recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913569,1610AC16.4% 425.4 / 69.4 175D87A (GAC→GCC) ybcKDLP12 prophage; putative recombinase
Reads supporting (aligned to +/- strand):  ref base A (74/71);  new base C (15/14);  total (90/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

AAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCT  >  NC_000913/569062‑569259
                                                                                                   |                                                                                                  
aaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGa                                                                                                    >  2:2060619/1‑100 (MQ=255)
 aGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCACGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGAc                                                                                                   <  2:250295/100‑1 (MQ=255)
    aaaGCATGCACAATCAGGAGCTTTTTCGTAATTTTTAAATGCGATAGCGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAg                                                                                                 <  1:1767749/99‑1 (MQ=255)
    aaaGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGa                                                                                                >  2:3547748/1‑100 (MQ=255)
      aGCATGCACAATCAGGAGCTTTTTCGGAATGTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTACAGa                                                                                                >  1:4261792/1‑98 (MQ=255)
        cATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACttt                                                                                            >  1:3546412/1‑100 (MQ=255)
         aTGCACAATCAGGAGCTTTTTCGGATTTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTc                                                                                           <  1:1821643/100‑1 (MQ=255)
          tGCACAATCAGGAGCTTTTTTGTTTTTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCa                                                                                          >  1:1501265/1‑100 (MQ=255)
          tGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCa                                                                                          <  2:1935512/100‑1 (MQ=255)
          tGCACAATCAGGAGCTTTTTCGGAATGTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCa                                                                                          <  2:2557283/100‑1 (MQ=255)
           gCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCaa                                                                                         <  2:2493996/100‑1 (MQ=255)
           gCACAATCAGGAGCTTTTTCGGAATTTGTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaa                                                                                         <  1:3834836/100‑1 (MQ=255)
            cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  2:2079099/100‑2 (MQ=255)
            cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  1:904446/100‑2 (MQ=255)
            cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  2:1420533/100‑2 (MQ=255)
            cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAag                                                                                        >  1:1115300/1‑100 (MQ=255)
            cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAag                                                                                        >  2:599188/1‑100 (MQ=255)
            cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAag                                                                                        >  2:719140/1‑100 (MQ=255)
            cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAag                                                                                        <  1:2669062/100‑1 (MQ=255)
             acaATGAGGAGCTTTTTCGGATTTTTTAGAGGCTATTGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAag                                                                                        <  1:2874485/99‑1 (MQ=255)
             acaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCAaga                                                                                       >  2:1354126/1‑100 (MQ=255)
              caATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  1:1275816/98‑2 (MQ=255)
              caATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAagag                                                                                      >  2:92537/1‑100 (MQ=255)
                aTCAGGATCTTTCTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTCCCAGCAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTc                                                                                           <  1:3706212/93‑1 (MQ=255)
                aTCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGaa                                                                                    >  2:1410344/1‑100 (MQ=255)
                 tCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGaaa                                                                                   >  1:1435701/1‑100 (MQ=255)
                        cTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCgg                                                                            >  2:2171935/1‑100 (MQ=255)
                        cTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCgg                                                                            <  2:1538838/100‑1 (MQ=255)
                              cGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGc                                                                      >  2:1239439/1‑100 (MQ=255)
                               ggAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCg                                                                     >  2:2250755/1‑100 (MQ=255)
                               ggAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCg                                                                     >  1:3336959/1‑100 (MQ=255)
                                gAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGa                                                                    >  1:2404030/1‑100 (MQ=255)
                                 aaTTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAt                                                                   >  2:2361889/1‑100 (MQ=255)
                                  aTTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAtt                                                                  <  2:696892/100‑1 (MQ=255)
                                    ttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGa                                                                >  2:3672007/1‑100 (MQ=255)
                                     tttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGaa                                                               <  2:496774/100‑1 (MQ=255)
                                        aGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGt                                                            >  1:872344/1‑100 (MQ=255)
                                         gATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTc                                                           <  2:3392328/100‑1 (MQ=255)
                                         gATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTc                                                           <  1:3195285/100‑1 (MQ=255)
                                         gATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTc                                                           >  1:1257319/1‑100 (MQ=255)
                                             cTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCAATTGAACGTCTGaa                                                       >  1:2909506/1‑100 (MQ=255)
                                              tataGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGaaa                                                      >  1:2041433/1‑100 (MQ=255)
                                              tataGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGaaa                                                      >  1:2835897/1‑100 (MQ=255)
                                               ataGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGATAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAAt                                                     <  1:2377043/100‑1 (MQ=255)
                                               ataGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAAt                                                     <  2:2962349/100‑1 (MQ=255)
                                               ataGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAAt                                                     <  2:3913419/100‑1 (MQ=255)
                                                taGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAAtt                                                    >  2:4242128/1‑100 (MQ=255)
                                                  gagCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGa                                                  >  2:1379293/1‑100 (MQ=255)
                                                   agCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGAt                                                 <  2:2976198/100‑1 (MQ=255)
                                                    gCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGAtt                                                <  1:1354122/100‑1 (MQ=255)
                                                    gCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGAtt                                                <  2:151250/100‑1 (MQ=255)
                                                      aTGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGAtttt                                              >  1:3408199/1‑100 (MQ=255)
                                                       tGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTg                                             >  2:3072347/1‑100 (MQ=255)
                                                        ggTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGa                                            >  1:693990/1‑100 (MQ=255)
                                                         gTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGaa                                           >  1:3028711/1‑100 (MQ=255)
                                                         gTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGaa                                           <  1:3229879/100‑1 (MQ=255)
                                                          ttATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAAt                                          <  2:3304474/100‑1 (MQ=255)
                                                             tatatTGCCAGGAACTACATGGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTAAACGTCTGAAATTGATTTTGAATCAc                                       >  1:4005388/1‑100 (MQ=255)
                                                               tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACgg                                     >  2:2923449/1‑100 (MQ=255)
                                                               tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACgg                                     <  2:3441584/100‑1 (MQ=255)
                                                               tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACgg                                     >  2:560436/1‑100 (MQ=255)
                                                                  tGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTAt                                  >  1:2510412/1‑100 (MQ=255)
                                                                    ccAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTg                                >  1:2740231/1‑100 (MQ=255)
                                                                       ggAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATg                             <  2:4170781/100‑1 (MQ=255)
                                                                        gAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGt                            >  2:2776128/1‑100 (MQ=255)
                                                                        gAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGt                            <  1:1094316/100‑1 (MQ=255)
                                                                          aCTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTta                          <  2:3798222/100‑1 (MQ=255)
                                                                           cTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTtat                         <  2:1864816/100‑1 (MQ=255)
                                                                            tACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTtata                        <  2:652110/100‑1 (MQ=255)
                                                                             tcATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAATAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTCGAATCACGGTATTGATGTTATaa                       >  1:122772/2‑100 (MQ=255)
                                                                             aCATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATaa                       <  2:2041437/100‑1 (MQ=255)
                                                                              naTTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAAc                      >  1:2974234/2‑100 (MQ=255)
                                                                              cATTGTTAGTTGAAAGTCTGGTCAGCCTTTCAAGAGAAAAAGTCGGTGAAGCAATTGAACGTCTGAAATTGATATTGAATAAAGGTATTGATGTta                          >  1:3384101/1‑96 (MQ=255)
                                                                                  gTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCtt                  >  2:1106266/1‑100 (MQ=255)
                                                                                   ttAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCttt                 <  1:2171930/100‑1 (MQ=255)
                                                                                    tAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTg                <  1:1033459/100‑1 (MQ=255)
                                                                                    ctcttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTg                >  2:3528342/4‑100 (MQ=39)
                                                                                     tcttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               >  2:1102105/3‑100 (MQ=255)
                                                                                     tcttGAAAGTCTGTCAAGACTTTAAAGAGAAAAAGTCGGTGAAGCTATTGAACGTCTGAAAATGATTTTGAATCACGTTATTGATGTTATAACACTTTGc               >  1:3912182/3‑100 (MQ=255)
                                                                                     aGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               <  1:1579465/100‑1 (MQ=255)
                                                                                     aGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               >  1:4082258/1‑100 (MQ=255)
                                                                                     aGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               <  2:3490630/100‑1 (MQ=255)
                                                                                     aGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               <  2:763886/100‑1 (MQ=255)
                                                                                      gTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  2:311643/1‑100 (MQ=255)
                                                                                      gTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  2:1272054/1‑100 (MQ=255)
                                                                                      cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  2:17522/2‑100 (MQ=255)
                                                                                      cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  1:2454496/2‑100 (MQ=255)
                                                                                      cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  1:2202631/2‑100 (MQ=255)
                                                                                      cttGAAAGTCTGACCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACAGTATTGATGTTATAAATCTTTg                >  1:817713/2‑98 (MQ=255)
                                                                                       ttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGa             >  1:3590830/1‑100 (MQ=255)
                                                                                        tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGGCTGAAATTGCTTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc            >  2:410887/1‑100 (MQ=255)
                                                                                        tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTCAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc            >  1:2269286/1‑100 (MQ=255)
                                                                                           aaGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAAt         <  1:1842908/100‑1 (MQ=255)
                                                                                             gTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATAc       >  2:1975828/1‑100 (MQ=255)
                                                                                              tCTGGACAGACTTTCAAGATAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACa      <  1:1529658/100‑1 (MQ=255)
                                                                                              tCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACa      >  1:556037/1‑100 (MQ=255)
                                                                                               cTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAg     <  2:3916780/100‑1 (MQ=255)
                                                                                                tGGACAGACTTGCAAGAGAAAGAGTCGGTGAAGCGATTGGACGTCTTAAATTGATTTTGAATCACGGTATTGATGTTATAACTATTTGCGACAATACAGt    <  1:3466612/100‑1 (MQ=255)
                                                                                                 ggACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTc   <  1:719137/100‑1 (MQ=255)
                                                                                                 ggACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTc   >  1:3216634/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
AAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCT  >  NC_000913/569062‑569259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: