Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,705 C→T 15.7% intergenic (+42/+18) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,7050CT15.7% 387.0 / 54.6 161intergenic (+42/+18)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base C (76/59);  new base T (14/11);  total (90/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 172 total aligned reads displayed.

TTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGC  >  NC_000913/681607‑681803
                                                                                                  |                                                                                                  
ttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccg                                                                                                   <  2:1521626/100‑1 (MQ=255)
ttAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccg                                                                                                   >  2:511627/1‑100 (MQ=255)
 tAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcgccgc                                                                                                  >  1:3007526/1‑100 (MQ=255)
  aaCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCa                                                                                                 >  2:3706703/1‑100 (MQ=255)
   aCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTGGCTGACGATGTGGTTTTTGCGCCGATGCCGGAGGCAGCGTTGAcgccgc                                                                                                  >  2:1916877/1‑98 (MQ=255)
   aCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCAt                                                                                                <  2:4105573/100‑1 (MQ=255)
   aCGGTGCTGGGAATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCAt                                                                                                <  1:3633665/100‑1 (MQ=255)
    cGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATc                                                                                               >  1:488199/1‑100 (MQ=255)
    cGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATc                                                                                               >  1:3076882/1‑100 (MQ=255)
     ggTGCTGGTGATAGGTTATGTTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATcc                                                                                              >  1:2614805/1‑100 (MQ=255)
      gTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCg                                                                                             <  2:714670/100‑1 (MQ=38)
       tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                            <  2:3250775/100‑1 (MQ=38)
       tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                            <  2:2427191/100‑1 (MQ=255)
       tGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTGTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                            <  1:2963289/100‑1 (MQ=38)
       tGCTGGGGATAGGTTATGCTCTCGGCAAAGTCGCGATGGTGTGTAGCTGACGATGGGTTTTTTGCGGCGAGGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                            <  1:832396/100‑1 (MQ=25)
        gCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGc                                                                                           <  2:2565760/100‑1 (MQ=38)
           ggTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGCatc                                                                                        <  1:3238809/100‑3 (MQ=37)
           ggTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGc                                                                                        <  2:3923273/100‑1 (MQ=255)
           ggTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGc                                                                                        <  1:2299874/100‑1 (MQ=255)
            gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                       >  1:1987766/1‑100 (MQ=255)
             tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCg                                                                                      >  1:1759622/1‑100 (MQ=255)
                tAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgtgt                                                                                   >  2:1480116/1‑100 (MQ=255)
                  ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGttt                                                                                 >  2:2236636/1‑100 (MQ=255)
                  ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGAAGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCGGCCGGGttt                                                                                 >  1:2091154/1‑100 (MQ=255)
                  ggTTATGCTCTCGGCAAAATCGCGATGTTGGTTAGCTGACGATGTATTTTTTGCGCCGATGCCGGATGCAGCGCTTACGCCGCATCCGGCAGCCgtg                                                                                    >  1:1021084/1‑97 (MQ=255)
                     tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                              <  2:1987770/100‑1 (MQ=255)
                     tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                              <  1:3401339/100‑1 (MQ=255)
                     tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATg                                                                              >  2:2839541/1‑100 (MQ=255)
                       tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                            <  2:863392/100‑1 (MQ=255)
                       tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                            <  1:4192122/100‑1 (MQ=255)
                        gCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGa                                                                           <  2:3417283/100‑1 (MQ=255)
                         ctctCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                          >  1:2054819/1‑100 (MQ=255)
                         ctctCGGCAAAATCACCATGTTGTTTATCTTACTATGTCTTTTCTGCGCCAATGCCTTATGCAACTTTTACGCCGCATCCAGCAGCCCTGTTTATtgg                                                                            <  1:3554213/98‑1 (MQ=255)
                          tctcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtc                                                                         >  2:1451953/1‑100 (MQ=255)
                           ctcGGCAAAATCGCGGGGTTGGTGAGCTGACGATGTGTTTTTTGCTCGGTTGCCGGATGCAGCGTTTACGCCGCGTCCGGCAGCCGTGTTTGTGGGAtct                                                                        >  1:4006226/1‑100 (MQ=255)
                           ctcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtct                                                                        >  1:1796829/1‑100 (MQ=255)
                             cGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCa                                                                      >  1:4188418/1‑100 (MQ=255)
                             cGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCa                                                                      >  2:3537695/1‑100 (MQ=255)
                               gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                                    >  2:2626065/1‑100 (MQ=255)
                               gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                                    >  2:4107229/1‑100 (MQ=255)
                               gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                                    >  2:165821/1‑100 (MQ=255)
                                    aTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATgg                                                                  >  1:2101714/1‑97 (MQ=255)
                                    aTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGCTTATGGGATCTCAATGGCTa                                                               >  2:2806199/1‑100 (MQ=255)
                                      cgcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTaaa                                                             <  2:2926746/100‑1 (MQ=255)
                                       gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                            <  2:765871/100‑1 (MQ=255)
                                       gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                            >  2:2422288/1‑100 (MQ=255)
                                       gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                            >  2:1088237/1‑100 (MQ=255)
                                       gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaat                                                            <  2:1165393/100‑1 (MQ=255)
                                           tgttgtTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCTGTGTTTATGGGATCTCAATGGCTAaataacc                                                        <  2:2889126/100‑3 (MQ=255)
                                            gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                                       <  2:364740/100‑1 (MQ=255)
                                             ttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgc                                                      <  1:972633/100‑1 (MQ=255)
                                              tgttTAGCTGACGATGTGTTTTTTGCTCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAAGAAtcgct                                                     >  1:124290/1‑100 (MQ=255)
                                                tttAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctcg                                                   <  1:2386616/100‑1 (MQ=255)
                                                tttAGCTGAAGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctcg                                                   <  1:1473912/100‑1 (MQ=255)
                                                    gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                               <  2:371566/100‑1 (MQ=255)
                                                    gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                               >  2:2115866/1‑100 (MQ=255)
                                                    gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCAGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccg                                               >  1:2262601/1‑100 (MQ=255)
                                                             gtgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAAc                                      <  1:3531518/100‑1 (MQ=255)
                                                               gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                                    <  2:3391365/100‑1 (MQ=255)
                                                                ttttttGCGCCGATGCCGGATGCAGCGTTTACGCGGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTATATCATCGCTCGCCCGCCGCCGAACtg                                    <  1:2180417/99‑2 (MQ=255)
                                                                  ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                                 <  2:2656363/100‑1 (MQ=255)
                                                                  ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCAt                                 <  2:603230/100‑1 (MQ=255)
                                                                        gcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcgc                           >  1:3241727/1‑100 (MQ=255)
                                                                         ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcgcg                          >  2:3021193/1‑100 (MQ=255)
                                                                            ctGCCGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGc                       >  1:2571795/2‑100 (MQ=37)
                                                                            aTGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGc                       >  2:2109406/1‑100 (MQ=255)
                                                                              gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa                     <  1:1982837/100‑1 (MQ=255)
                                                                              gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCa                     <  1:3744844/100‑1 (MQ=255)
                                                                               ncGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat                    >  2:1767819/2‑100 (MQ=255)
                                                                               ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat                    >  2:3975801/1‑100 (MQ=38)
                                                                               ccGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat                    >  1:1139618/1‑100 (MQ=38)
                                                                               ccGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat                    >  2:3187309/1‑100 (MQ=255)
                                                                                cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata                   >  2:3305976/1‑100 (MQ=38)
                                                                                cGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata                   >  2:1248142/1‑100 (MQ=38)
                                                                                cGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGACGCCGAACTTCATCCACGCGGGcc                      >  1:756930/1‑97 (MQ=255)
                                                                                 ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat                  >  1:1032941/1‑100 (MQ=38)
                                                                                 ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat                  >  1:1388409/1‑100 (MQ=38)
                                                                                  gATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATc                 >  2:3857468/1‑100 (MQ=255)
                                                                                  gATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATc                 <  2:354154/100‑1 (MQ=255)
                                                                                   aTGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCg                <  1:3969506/100‑1 (MQ=255)
                                                                                    tGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGt               >  2:3147402/1‑100 (MQ=255)
                                                                                      cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt             >  1:2532217/1‑100 (MQ=255)
                                                                                       aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg            >  2:1221477/1‑100 (MQ=255)
                                                                                       aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg            >  2:3802859/1‑100 (MQ=255)
                                                                                       aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg            <  1:3678241/100‑1 (MQ=255)
                                                                                       aGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCAGCCGAACTTCATCCACGCGGGCCATATCGTTTg            <  1:2819628/100‑1 (MQ=255)
                                                                                        gcattttggccgcTTCCGGCAGCCGTCGATTTGGGATGTCAATGGCTAAATAATCGATTGCCAGCCGACGAACTTCATCCACGCGGGCCATATCGttt             <  1:2658921/90‑1 (MQ=255)
                                                                                         cGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc          >  2:1270200/1‑100 (MQ=255)
                                                                                         cGTTAACGCCGCATCCGGCAGCCGTGTTTATGGCATCTCAATGGCTAAATAATCGATCGCCCGCCTCCGAACTTCATCCACGCGGGCCATATCCTTTGAc          <  1:1617256/100‑1 (MQ=255)
                                                                                          gTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACt         >  2:4013699/1‑100 (MQ=255)
                                                                                           tttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTg        <  2:2176927/100‑1 (MQ=255)
                                                                                           tttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTg        >  2:2341319/1‑100 (MQ=255)
                                                                                            ttACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGc       <  1:1598645/100‑1 (MQ=255)
                                                                                             tACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCa      <  1:3732915/100‑1 (MQ=255)
                                                                                              aCGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCaa     >  1:658795/1‑100 (MQ=255)
                                                                                              aCGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCaa     >  1:2115451/1‑100 (MQ=255)
                                                                                                gccgcATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcg   >  2:3297218/1‑100 (MQ=255)
                                                                                                gccgcATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcg   >  2:1901582/1‑100 (MQ=255)
                                                                                                gccgcATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcg   >  2:2935955/1‑100 (MQ=255)
                                                                                                 ccgcATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcgc  >  2:566840/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
TTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGC  >  NC_000913/681607‑681803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: