Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 700,805 A→T 100% C264S (TGC→AGC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913700,8050AT97.9% 467.3 / ‑0.0 142C264S (TGC→AGC) nagCN‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (74/65);  total (76/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

Only 100 of 142 total aligned reads displayed.

GTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCC  >  NC_000913/700708‑700901
                                                                                                 |                                                                                                
gTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCACTGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGc                                                                                                >  1:1875986/1‑100 (MQ=255)
gTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGc                                                                                                <  1:4115785/100‑1 (MQ=255)
  gCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCa                                                                                              >  1:1331120/1‑100 (MQ=255)
  gCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCa                                                                                              <  2:243423/100‑1 (MQ=255)
     gtcgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAg                                                                                           >  2:568231/1‑100 (MQ=255)
     gtcgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAg                                                                                           >  2:444948/1‑100 (MQ=255)
      tcgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGt                                                                                          >  1:2260742/1‑100 (MQ=255)
      tcgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGt                                                                                          <  1:2169536/100‑1 (MQ=255)
        gtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTg                                                                                        >  1:912172/1‑100 (MQ=255)
         tcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGc                                                                                       <  1:388720/100‑1 (MQ=255)
          ccAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGcc                                                                                      <  1:3365736/100‑1 (MQ=255)
          ccAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGcc                                                                                      <  2:659939/100‑1 (MQ=255)
           cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGcc                                                                                      >  2:2946848/1‑99 (MQ=255)
           cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCg                                                                                     >  1:2359177/1‑100 (MQ=255)
           cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCg                                                                                     >  2:3741208/1‑100 (MQ=255)
           cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCg                                                                                     <  1:254437/100‑1 (MQ=255)
           cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCg                                                                                     >  2:2946783/1‑100 (MQ=255)
           cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCg                                                                                     >  2:4141560/1‑100 (MQ=255)
           cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTAGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCg                                                                                     >  2:3623927/1‑100 (MQ=255)
            aGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGAAGCGCTAGTTTCCAGGCTGCCAAAGTTGCCGc                                                                                    >  1:405356/1‑100 (MQ=255)
               ggCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGACAAAGTTGCcaca                                                                                   >  1:3311476/1‑98 (MQ=255)
                  aCGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGc                                                                              <  1:4141538/100‑1 (MQ=255)
                   cgcgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCa                                                                             >  2:3315485/1‑100 (MQ=255)
                    gcgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAg                                                                            <  1:3197605/100‑1 (MQ=255)
                      ggCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCg                                                                          >  1:3971536/1‑100 (MQ=255)
                       gCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGt                                                                         >  2:2715211/1‑100 (MQ=255)
                        ctctGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGtt                                                                        >  2:1168245/1‑100 (MQ=255)
                        ctctGGTAGCCCTGCTATGACAGATCAAACACCCTTTGTTCAATGGCAGCGTTGGCAGCGACAGTTTCCAGGCTGCCAAAGTAGCCGCAGTg                                                                                <  1:4278463/92‑1 (MQ=255)
                         tctGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTc                                                                       <  2:1162361/100‑1 (MQ=255)
                         tctGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTc                                                                       <  1:1116529/100‑1 (MQ=255)
                           tGGTAGCCCTGCTTTCACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGc                                                                           <  1:3942746/94‑1 (MQ=255)
                           ccgatctccTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCAc                                                                     <  1:1801209/93‑1 (MQ=255)
                            ggTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCAcc                                                                    >  1:239070/1‑100 (MQ=255)
                            ggTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGAGGCAACGTTCAcc                                                                    >  2:1479616/1‑100 (MQ=255)
                              tAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCa                                                                  >  2:4041636/1‑100 (MQ=255)
                               aGCCTTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGGTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTc                                                                       <  1:1170923/94‑1 (MQ=255)
                                 cccTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCg                                                               <  1:1914930/100‑1 (MQ=255)
                                  ccTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCAcagatcgg                                                              >  2:1801213/1‑93 (MQ=255)
                                   cTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGt                                                             <  1:2207756/100‑1 (MQ=255)
                                   cTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGt                                                             >  2:480301/1‑100 (MQ=255)
                                    tGCTTTAACAGATTCAACACCCGTTGTTCAATAGCGGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGtt                                                            >  1:1721340/1‑100 (MQ=255)
                                     gCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTc                                                           <  1:2070810/100‑1 (MQ=255)
                                        ttAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAc                                                        >  2:3086563/1‑100 (MQ=255)
                                         tAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcc                                                       <  2:2288678/100‑1 (MQ=255)
                                          aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCt                                                      <  1:4064167/100‑1 (MQ=255)
                                          aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCt                                                      >  2:262018/1‑100 (MQ=255)
                                          aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCt                                                      >  2:3336770/1‑100 (MQ=255)
                                           aCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTg                                                     >  2:1603524/1‑100 (MQ=255)
                                            cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcccga                                                    >  1:3472576/1‑97 (MQ=255)
                                            cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa                                                    <  2:1708033/100‑1 (MQ=255)
                                            cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa                                                    >  2:2681650/1‑100 (MQ=255)
                                             aGATTCAATAACCGTTGTTCAATGGCAGCGTGCGCAGCGATTGTTTCCAGGCTTCCAAAGTTGCCGCAGGTGCAACGTTCACCCCGCGTTTCGACCTGaa                                                   <  1:1903649/100‑1 (MQ=255)
                                             aGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGaa                                                   <  1:1850947/100‑1 (MQ=255)
                                              gATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAat                                                  <  2:859770/100‑1 (MQ=255)
                                               aTTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAata                                                 >  1:770492/1‑100 (MQ=255)
                                                ttCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAatat                                                >  1:4174799/1‑100 (MQ=255)
                                                ttCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAata                                                 >  2:1692177/1‑99 (MQ=255)
                                                 tCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATg                                               <  2:3337542/100‑1 (MQ=255)
                                                  cAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATgg                                              >  1:2816619/1‑100 (MQ=255)
                                                    acacCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGcc                                            >  2:3458666/1‑100 (MQ=255)
                                                      acCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCaa                                          >  1:4267159/1‑100 (MQ=255)
                                                       cccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAt                                         >  1:545870/1‑100 (MQ=255)
                                                       cccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGCGGCAGCGTTCACCCAGCGGTTCGACCTGAATACGGCCaa                                          >  1:1592990/1‑99 (MQ=255)
                                                        ccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAtt                                        >  2:2380170/1‑100 (MQ=255)
                                                        ccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAtt                                        <  2:3876911/100‑1 (MQ=255)
                                                          gttgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTc                                      <  1:311470/100‑1 (MQ=255)
                                                            tgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAc                                    <  2:1455174/100‑1 (MQ=255)
                                                             nttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc                                   >  1:3248665/2‑100 (MQ=255)
                                                             gttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc                                   <  1:1513722/100‑1 (MQ=255)
                                                             gttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc                                   <  2:3204575/100‑1 (MQ=255)
                                                              ttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAccn                                  <  2:2050748/100‑2 (MQ=255)
                                                              ttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCg                                  >  1:570029/1‑100 (MQ=255)
                                                               tCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCg                                  >  1:3425032/1‑99 (MQ=255)
                                                               tCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGa                                 >  2:1370889/1‑100 (MQ=255)
                                                                 aaTGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcg                               >  1:3455051/1‑100 (MQ=255)
                                                                   tGGCAGCGTTGGCAGCGATAGTTTCCAGGTTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgtt                             >  2:1027135/1‑100 (MQ=255)
                                                                    ggCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttg                            >  1:3214134/1‑100 (MQ=255)
                                                                    ggCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttg                            >  1:3384121/1‑100 (MQ=255)
                                                                     gCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgc                           <  2:2816630/100‑1 (MQ=255)
                                                                      cAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgcc                          >  1:1186794/1‑100 (MQ=255)
                                                                       aGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccg                         <  1:3633429/100‑1 (MQ=255)
                                                                        gCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgt                        <  2:3455064/100‑1 (MQ=255)
                                                                        gCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgt                        >  2:2166622/1‑100 (MQ=255)
                                                                        gCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgt                        <  2:719173/100‑1 (MQ=255)
                                                                        gCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccg                         <  2:3841074/99‑1 (MQ=255)
                                                                            tGGCAGCGATAGTTTCCATGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAAGATGGCCAATTTCACCGACGTTGCCGTTGCg                    <  2:3768607/100‑1 (MQ=255)
                                                                             ggCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCgg                   <  1:265527/100‑1 (MQ=255)
                                                                               cAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGcc                 <  2:4189356/100‑1 (MQ=255)
                                                                               cAGCGATAGTCTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGcc                 <  2:2161175/100‑1 (MQ=255)
                                                                                  ngATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAAt              >  1:745743/2‑100 (MQ=255)
                                                                                     tAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaa           >  1:2719233/1‑100 (MQ=255)
                                                                                      aGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaa          <  1:3682409/100‑1 (MQ=255)
                                                                                        tttCCAGGCTGCCAAAGTTGCCGCAGTTGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaaa         <  1:3308352/99‑1 (MQ=255)
                                                                                        tgtCCAGGCTGCCACAGTTGCCGCAGTGGCATCCTTCACCCCGCGGTTCGCCCTGTACATGGCCAATTTCACCTACGTTCTCGTTGCGGCCAATa             <  1:1296031/93‑1 (MQ=255)
                                                                                        nttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATAGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaca         >  1:1709111/2‑97 (MQ=255)
                                                                                         ttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATg       <  2:1017208/100‑1 (MQ=255)
                                                                                          tCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgc      <  2:1730665/100‑1 (MQ=255)
                                                                                           ccAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgcg     <  2:1661690/100‑1 (MQ=255)
                                                                                            cAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgcgc    >  2:2224683/1‑100 (MQ=255)
                                                                                              ggCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGccc  >  1:1597552/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCCC  >  NC_000913/700708‑700901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: