Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 850,995 T→G 15.1% intergenic (+30/+19) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913850,9950TG15.1% 376.9 / 56.9 155intergenic (+30/+19)ompX/opgEouter membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base T (70/61);  new base G (13/11);  total (83/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 161 total aligned reads displayed.

TTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCG  >  NC_000913/850896‑851093
                                                                                                   |                                                                                                  
ttACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCctct                                                                                                    <  1:4113765/100‑1 (MQ=255)
 tACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCtctc                                                                                                   >  2:534971/1‑100 (MQ=255)
   cGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCgg                                                                                                 >  2:1614914/1‑100 (MQ=255)
    gAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCggg                                                                                                >  1:3212210/1‑100 (MQ=255)
         gagCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGa                                                                                           <  2:4091864/100‑1 (MQ=255)
            ccGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAAGCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttt                                                                                        <  1:1255143/100‑1 (MQ=38)
             cGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAtttt                                                                                       <  1:1378802/100‑1 (MQ=255)
             cGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAtttt                                                                                       >  1:362064/1‑100 (MQ=255)
             cGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAtttt                                                                                       <  1:586301/100‑1 (MQ=255)
             cGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTCCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttt                                                                                       <  2:343623/100‑1 (MQ=38)
               tagtCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTg                                                                                     <  1:1332561/97‑1 (MQ=255)
               tATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTg                                                                                     <  1:1274171/100‑1 (MQ=255)
               tATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttttt                                                                                     <  1:1307744/100‑2 (MQ=38)
               tATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttttt                                                                                     <  2:2235195/100‑2 (MQ=38)
                aTTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGt                                                                                    >  1:1736625/1‑100 (MQ=255)
                 ttCGTAGCGTTGACGTAGGCACCTGGATTTCCGGTGTTGGTTACCGCTTCTAATCACGTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGt                                                                                    >  2:461503/1‑99 (MQ=255)
                  tCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGttt                                                                                  <  1:1152301/100‑1 (MQ=255)
                  tCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAttttttata                                                                                  <  2:450917/100‑5 (MQ=37)
                    gTAGCGTTGACGTAGGCACCTGGATTTCTTGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATGAAAAATCCGCCTCTCGGGGCGGATTTTTGtttt                                                                                 <  1:2784094/99‑1 (MQ=255)
                    gTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtttt                                                                                 <  2:4137645/99‑1 (MQ=255)
                    gTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGGTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCAAGGCGGACTTCTgat                                                                                   >  1:3997728/1‑95 (MQ=255)
                      aGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTaa                                                                              <  2:1738668/100‑1 (MQ=255)
                       gCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAg                                                                             <  1:968805/100‑1 (MQ=255)
                        ctttGACTTAGGCACCTGGATGGCCGGTTTTGGTTACCGCTTCTAATCACTTTGGTGAGTTAAACAAGCCGCCTCTCGGGGCGGATTTTTg                                                                                     <  1:1748031/89‑1 (MQ=255)
                        cGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAgg                                                                            >  2:962070/1‑100 (MQ=255)
                        cGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAgg                                                                            <  1:3036648/100‑1 (MQ=255)
                         gTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGt                                                                           >  1:1427479/1‑100 (MQ=255)
                             aCGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCg                                                                       <  1:725781/100‑1 (MQ=255)
                               gTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCggg                                                                     <  2:990882/100‑1 (MQ=255)
                                 aGGCACCTGGATTGCCGGTTTTGGTTACCGCTTCTAATCACTTTTGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGt                                                                    <  1:1845466/99‑1 (MQ=255)
                                  ggCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTc                                                                   >  1:1227494/1‑99 (MQ=255)
                                  ggCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCAGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCg                                                                  <  2:3997748/100‑1 (MQ=255)
                                    cACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGCCGat                                                                >  1:249768/1‑99 (MQ=255)
                                      ccTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGaaaa                                                              <  1:1487405/100‑1 (MQ=255)
                                        tGGATTGCCTGTGTTGGTTACCGCTTCTAATCACTTTGGTTATATAAAAAATCCGCCTCGCGGGGCGGATTTTTGTTTTTAAGGGTTCGGGTCGaaaa                                                              <  1:2636659/98‑1 (MQ=255)
                                           aTTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCg                                                         >  2:3176961/1‑100 (MQ=255)
                                              gCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGtt                                                      >  2:3302992/1‑100 (MQ=255)
                                                 ggTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCg                                                   <  1:2554382/100‑1 (MQ=255)
                                                 ggTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCg                                                   >  1:2466568/1‑100 (MQ=255)
                                                  gtgtTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCGCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCga                                                  <  1:309725/100‑1 (MQ=255)
                                                    gtTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCgaga                                                <  1:1821059/100‑1 (MQ=255)
                                                    gtTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCgaga                                                >  2:3921503/1‑100 (MQ=255)
                                                       ggTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGCTTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCgagct                                               >  1:3740752/1‑96 (MQ=255)
                                                        gTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTACGAGATGGt                                            >  1:873926/1‑100 (MQ=255)
                                                          tACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTg                                          >  2:3834580/1‑100 (MQ=255)
                                                          tACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTg                                          <  1:4144893/100‑1 (MQ=255)
                                                            ccGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGt                                         <  1:2235618/99‑1 (MQ=255)
                                                            ccGCTTCTAATCACTTTGGTGATATAAAAAATCCGCATCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTa                                        >  2:3012188/1‑100 (MQ=255)
                                                              gCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAAt                                      <  1:3486484/100‑1 (MQ=255)
                                                              gCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAAt                                      >  1:181540/1‑100 (MQ=255)
                                                              gCTTCGAATTACTGTGGTGATATAAAAAAGCCGCCTCTCGGGGCTGAGTTGTGTTTGTAAGGTTTCGGGTCGAAAATATCGGTGCCGAGATGGTTGt                                         <  1:3703472/97‑1 (MQ=255)
                                                                   tAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCAcc                                 <  2:480783/100‑1 (MQ=255)
                                                                    aaTCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCt                                >  1:2920221/1‑100 (MQ=255)
                                                                    aaTCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCt                                >  2:2526245/1‑100 (MQ=255)
                                                                    aaTCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCt                                >  2:2346220/1‑100 (MQ=255)
                                                                    aaTCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCt                                >  2:675913/1‑100 (MQ=255)
                                                                       cACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCt                             <  1:453196/100‑1 (MQ=255)
                                                                        aCTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTg                            >  2:75234/1‑100 (MQ=255)
                                                                         cTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTg                            <  1:734044/99‑1 (MQ=255)
                                                                         cTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTg                            <  1:892150/99‑1 (MQ=255)
                                                                         cTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGt                           >  2:2112926/1‑100 (MQ=255)
                                                                           ttGGTGGTATAAAAACTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTa                          <  1:1051501/99‑1 (MQ=255)
                                                                           ttGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTaa                         <  2:181540/100‑1 (MQ=255)
                                                                            tGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTaa                         >  2:2413348/1‑99 (MQ=255)
                                                                              ttaaaaacaaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTg                      >  2:1819683/9‑100 (MQ=25)
                                                                              ttaaaaacaaaaaTCCGCCCCGAGAGGCGGATTTTTGTATTTAAGGTTTCGGGCCGAAAAGATCGGTCCCGAGATGGTTGTAATCCACCTTCTGTAACt                       >  2:1960516/9‑99 (MQ=21)
                                                                              gTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAAc                        >  1:1348609/1‑98 (MQ=255)
                                                                              gTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTg                      <  1:2121873/100‑1 (MQ=255)
                                                                               tGATATATAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTgg                     >  2:2236991/1‑100 (MQ=255)
                                                                                gttataAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGa                    <  1:1757017/98‑1 (MQ=255)
                                                                                gATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTg                      >  1:2026477/1‑98 (MQ=255)
                                                                                aaaaacaaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGa                    >  2:532301/7‑100 (MQ=25)
                                                                                  aaacaaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTGTCGGGTCAAAAAAAACGGTTCAGAAATGAAGGTAATAGACCTTCAGTAAGTTGAAg                  >  1:1037274/5‑100 (MQ=9)
                                                                                   ataAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGt                 <  1:2349073/100‑1 (MQ=255)
                                                                                   ataAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGt                 >  1:3322004/1‑100 (MQ=255)
                                                                                    taAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGtt                >  2:4072913/1‑100 (MQ=255)
                                                                                    taAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGtt                >  1:2260082/1‑100 (MQ=255)
                                                                                    acaaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGtt                >  2:847296/3‑100 (MQ=38)
                                                                                     naaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTg               >  2:2208554/2‑100 (MQ=255)
                                                                                     caaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGt                 >  1:1579265/2‑98 (MQ=38)
                                                                                     caaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTg               >  2:1563622/2‑100 (MQ=38)
                                                                                     caaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTg               >  2:2929672/2‑100 (MQ=38)
                                                                                     caaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTg               >  1:442570/2‑100 (MQ=38)
                                                                                     aaaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGt                 >  1:2723220/1‑98 (MQ=255)
                                                                                     aaaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTg               >  2:285461/1‑100 (MQ=255)
                                                                                     aaaaaaTCCGCCTCTCGGGACGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAACATCGGTTCCGAGATGGTTGTAATCCACCTCCTGTAACTgcacg                  >  1:3640359/1‑93 (MQ=255)
                                                                                      aaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg              >  2:1389762/1‑100 (MQ=255)
                                                                                      aaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg              <  2:3107760/100‑1 (MQ=255)
                                                                                      aaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg              <  2:2770993/100‑1 (MQ=255)
                                                                                       aaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt             <  1:1445968/100‑1 (MQ=255)
                                                                                       aaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt             >  2:1430887/1‑100 (MQ=39)
                                                                                         aaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGa           >  1:2688176/1‑100 (MQ=255)
                                                                                          aTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGa           <  2:3665159/99‑1 (MQ=255)
                                                                                           tCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTGGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATg         >  1:2332766/1‑100 (MQ=255)
                                                                                           tCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATg         <  2:2424363/100‑1 (MQ=255)
                                                                                           tCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATg         <  2:638211/100‑1 (MQ=255)
                                                                                             cGCCTCTCGGTGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGta       <  2:352146/100‑1 (MQ=255)
                                                                                               ccTCTCGGGGCGGATTTTTGTTTTTAAGGTTTTGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGtata     <  2:362066/100‑1 (MQ=255)
                                                                                               ccTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGtata     >  1:2098723/1‑100 (MQ=255)
                                                                                                  ctcGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCg  >  1:3419341/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCG  >  NC_000913/850896‑851093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: