Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 882,856 C→A 47.7% R178L (CGT→CTT)  ybjG ← undecaprenyl pyrophosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913882,8560CA47.7% 46.8 / 219.8 153R178L (CGT→CTT) ybjGundecaprenyl pyrophosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (45/35);  new base A (34/39);  total (79/74)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

AGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGT  >  NC_000913/882757‑882955
                                                                                                   |                                                                                                   
 gCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACg                                                                                                    >  2:3883407/1‑100 (MQ=255)
  cGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGt                                                                                                   <  2:4164091/100‑1 (MQ=255)
   gAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGtn                                                                                                  <  1:1147470/100‑2 (MQ=255)
     gCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTg                                                                                                 <  2:2325732/99‑1 (MQ=255)
       cGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATa                                                                                              <  2:3523217/100‑1 (MQ=255)
        gCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATag                                                                                             >  1:3782600/1‑100 (MQ=255)
        gCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATag                                                                                             <  2:1808456/100‑1 (MQ=255)
        gCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATag                                                                                             >  2:1383726/1‑100 (MQ=255)
        gCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTCTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATag                                                                                             <  1:1871504/100‑1 (MQ=255)
        gCTAAATACTACACGTTTTAGCAGACATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCTCGATTGCAGAAGTTGAt                                                                                               <  1:599078/98‑1 (MQ=255)
          tCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATagag                                                                                           <  2:1707197/100‑1 (MQ=255)
           cAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGt                                                                                          <  1:1056564/100‑1 (MQ=255)
            aaTACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGtt                                                                                         >  2:1955852/1‑100 (MQ=255)
            aaTACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGtt                                                                                         <  1:1016893/100‑1 (MQ=255)
             aTACTACACTTTTTAGCAGAGATCAGTCACGCACTCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATTCCACGATTGCAGAAGTTGATAGAGt                                                                                          <  1:923644/98‑1 (MQ=255)
             aTACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGttt                                                                                        >  2:288304/1‑100 (MQ=255)
             aTACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGttt                                                                                        >  2:2713084/1‑100 (MQ=255)
              tactacACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATagatcgga                                                                                       >  2:3525908/1‑95 (MQ=255)
               actacACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTAt                                                                                      >  1:4201835/1‑100 (MQ=255)
               actacACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACCATTGCAGACGCTGATAGAGATTAt                                                                                      >  1:2044610/1‑100 (MQ=255)
                  acacTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGcc                                                                                   >  2:322380/1‑100 (MQ=255)
                   cacTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGccc                                                                                  >  2:2719483/1‑100 (MQ=255)
                   cacTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGccc                                                                                  <  2:3763159/100‑1 (MQ=255)
                     cTTTTTAGCGGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCa                                                                                <  1:2042788/100‑1 (MQ=255)
                     cTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCa                                                                                >  2:2052336/1‑100 (MQ=255)
                     cTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCa                                                                                >  2:2048632/1‑100 (MQ=255)
                       ttttAGCAGAGATCAGTCACTCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATc                                                                              >  1:2618559/1‑100 (MQ=255)
                       ttttAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATc                                                                              >  1:1825857/1‑100 (MQ=255)
                         ttAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGc                                                                            <  2:2622417/100‑1 (MQ=255)
                          tAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCt                                                                           >  2:73226/1‑100 (MQ=255)
                           aGCAGAGATCAGTCACGCACGCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCtt                                                                          >  1:2611407/1‑100 (MQ=255)
                            gCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTg                                                                         <  2:1571984/100‑1 (MQ=255)
                            gCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTg                                                                         <  2:2773767/100‑1 (MQ=255)
                            gCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTg                                                                         <  2:1480605/100‑1 (MQ=255)
                             cAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGc                                                                        <  1:2174789/100‑1 (MQ=255)
                             cAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGc                                                                        >  2:3591973/1‑100 (MQ=255)
                             cAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGc                                                                        >  1:1286957/1‑100 (MQ=255)
                              agagATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCACTTGcc                                                                       >  1:1496242/1‑100 (MQ=255)
                               gagaTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCa                                                                      <  1:213264/100‑1 (MQ=255)
                                agaTCAGTCACGCACCCAGCCTTTGCGGATCGGTACTGAAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCa                                                                      <  1:3405747/99‑1 (MQ=255)
                                agaTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAg                                                                     <  2:3986121/100‑1 (MQ=255)
                                 gaTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGa                                                                    >  2:2912295/1‑100 (MQ=255)
                                 gaTCAGTCACGCACCCAGCCTTTGCGGATCGGTAAGGCAAAACAGACGCGATACCAGGATTGCAGAAGTTGATAGAGTCTATGCCCCATCGCTTGCCAGa                                                                    <  1:677534/100‑1 (MQ=255)
                                  aTCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGAt                                                                   >  2:2338359/1‑100 (MQ=255)
                                      gTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATc                                                               >  1:100741/1‑100 (MQ=255)
                                       tCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTACAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATc                                                               <  1:3180264/99‑1 (MQ=255)
                                        cACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCGAGATAATCTg                                                             >  1:2817525/1‑100 (MQ=255)
                                        cACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTg                                                             >  1:958233/1‑100 (MQ=255)
                                         aCGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTgg                                                            <  2:2129341/100‑1 (MQ=255)
                                         aCGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTgg                                                            <  1:1314254/100‑1 (MQ=255)
                                           gCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGc                                                          <  1:3760009/100‑1 (MQ=255)
                                            cACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCa                                                         <  1:894501/100‑1 (MQ=255)
                                             aCCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCAc                                                        <  1:171449/100‑1 (MQ=255)
                                              cccAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACt                                                       <  1:4115095/100‑1 (MQ=255)
                                              cccAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATAACACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGTCACt                                                       >  2:1154007/1‑100 (MQ=255)
                                               ncAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTa                                                      >  2:159638/2‑100 (MQ=255)
                                               ccAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTa                                                      >  2:783503/1‑100 (MQ=255)
                                                    cTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCa                                                 <  2:2559792/100‑1 (MQ=255)
                                                    cTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCa                                                 >  2:811373/1‑100 (MQ=255)
                                                       tGCGGATCGGTAATGCAAGACAGGCGCGAGACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAg                                                <  1:3528447/98‑1 (MQ=255)
                                                       tGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGcc                                              <  2:2143263/100‑1 (MQ=255)
                                                       tGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGcc                                              >  2:310944/1‑100 (MQ=255)
                                                         cGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCaa                                            >  2:3294703/1‑100 (MQ=255)
                                                         cGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCaa                                            >  2:2660862/1‑100 (MQ=255)
                                                          ggATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAAt                                           <  1:1800944/100‑1 (MQ=255)
                                                            aTCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCa                                         <  2:1081734/100‑1 (MQ=255)
                                                            aTCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCa                                         <  1:3294692/100‑1 (MQ=255)
                                                               ggTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATAc                                      >  2:3471297/1‑100 (MQ=255)
                                                               ggTAATGCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATAc                                      <  2:384668/100‑1 (MQ=255)
                                                                    tGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGcc                                 >  2:1425027/1‑100 (MQ=255)
                                                                     gCAAAACAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCa                                >  2:473347/1‑100 (MQ=255)
                                                                      cAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAg                               >  2:1324724/1‑100 (MQ=255)
                                                                      cAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAg                               >  2:3701474/1‑100 (MQ=255)
                                                                      cAAAACAGACGCGATACAACGATTGCAGCCGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATACACTGGGCACTAAGGCAAACAATCATACATACCa                                >  1:569559/1‑99 (MQ=255)
                                                                       gaaaCAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGc                              >  1:2983819/2‑100 (MQ=255)
                                                                       aaaaCAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCa                                >  1:2394015/1‑98 (MQ=255)
                                                                        aaaCAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCa                             >  1:3321388/1‑100 (MQ=255)
                                                                          aCAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAAt                           >  2:1688045/1‑100 (MQ=255)
                                                                           cAGACGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATc                          <  2:1334573/100‑1 (MQ=255)
                                                                             gACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCa                        <  2:2517757/100‑1 (MQ=255)
                                                                             gACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCa                        <  2:1651386/100‑1 (MQ=255)
                                                                              aCGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCAc                       >  2:1710627/1‑100 (MQ=255)
                                                                              aCGCGATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCAc                       >  2:595038/1‑100 (MQ=255)
                                                                               cgcgATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCAcc                      >  1:475095/1‑100 (MQ=255)
                                                                               cgcgATACCACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCAcc                      >  2:1026509/1‑100 (MQ=255)
                                                                                 cgATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCg                     >  1:4227103/1‑99 (MQ=255)
                                                                                 cgATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGa                    >  2:3558177/1‑100 (MQ=255)
                                                                                  gATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAg                   <  1:1955848/100‑1 (MQ=255)
                                                                                  gATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAg                   <  2:526193/100‑1 (MQ=255)
                                                                                   aTACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGc                  <  2:1778920/100‑1 (MQ=255)
                                                                                    tACCACGATTGCAGAAGTTGATAGAGTTTATGCCCTATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCa                 <  1:2379925/100‑1 (MQ=255)
                                                                                      ccACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCata               >  1:2233344/1‑100 (MQ=255)
                                                                                      ccACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCata               <  2:716912/100‑1 (MQ=255)
                                                                                      ccACGATTGCAGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCata               <  2:479117/100‑1 (MQ=255)
                                                                                       cACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCatat              >  2:3104817/1‑100 (MQ=255)
                                                                                           aTTGCAGACGTTAATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATcc            >  1:1454894/1‑98 (MQ=255)
                                                                                              gCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCgg       >  2:4026526/1‑100 (MQ=255)
                                                                                               cAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGc      <  1:1181472/100‑1 (MQ=255)
                                                                                                aGAAGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGcc     >  1:1217658/1‑100 (MQ=255)
                                                                                                   cGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGt  <  2:891491/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGT  >  NC_000913/882757‑882955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: