Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,022,600 C→T 40.1% Q428Q (CAG→CAA yccS ← putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,022,6000CT40.1% 112.8 / 166.1 155Q428Q (CAG→CAAyccSputative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (42/50);  new base T (31/31);  total (74/81)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 170 total aligned reads displayed.

TGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCGACATTCTT  >  NC_000913/1022501‑1022699
                                                                                                   |                                                                                                   
tGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTtggc                                                                                                     <  2:1168362/100‑1 (MQ=255)
tGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTtggc                                                                                                     <  1:3950656/100‑1 (MQ=255)
    ggCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTtggctggc                                                                                                 >  2:2357693/1‑100 (MQ=255)
    ggCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTtggctggc                                                                                                 <  1:3012595/100‑1 (MQ=255)
     gCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCa                                                                                                >  2:3982112/1‑100 (MQ=255)
      cacaAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAg                                                                                               >  1:4015513/1‑100 (MQ=255)
      cacaAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAg                                                                                               <  2:2417169/100‑1 (MQ=255)
      cacaAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAg                                                                                               <  2:3259488/100‑1 (MQ=255)
          aaCCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGaca                                                                                            <  1:2658938/99‑1 (MQ=255)
           aCCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGacaaa                                                                                          <  1:3412233/100‑1 (MQ=255)
            ccACAGCACAGGCATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGTTGGCGCGTGGCGTTATAGTTTGGTTGGCAGacaaac                                                                                         >  1:798130/1‑100 (MQ=255)
            ccACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCACacaaac                                                                                         >  1:3254129/1‑100 (MQ=255)
              acagcacagGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGTCTGCCAGccaaacta                                                                                       <  2:89304/100‑3 (MQ=255)
               cagcacagGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGAcaaacaaa                                                                                      >  2:1883983/1‑100 (MQ=255)
               cagcacagGAATGCCAATGGCGATACCTACCAGCGTACCAATAAGCCTTAACTTCAGGCGTGGCGCGTGGCGTTATAGTTTTGCTGGCAGACaaacaaac                                                                                     <  2:1040523/100‑1 (MQ=255)
                agcacatGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACaaacaaac                                                                                     <  2:3834205/100‑1 (MQ=255)
                 gcacagGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACt                                                                                    >  2:3782260/1‑100 (MQ=255)
                 gcacagGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACt                                                                                    >  2:2684251/1‑100 (MQ=255)
                 gcacagGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACt                                                                                    <  2:3298130/100‑1 (MQ=255)
                  cacagGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGTCTGCCAGccaaactataac                                                                                   <  1:4051251/100‑7 (MQ=255)
                  cacagGAATGCCAATGGCGATACCTACCACCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACt                                                                                    <  2:945834/99‑1 (MQ=255)
                   acagGTATGCCAATGGCGATACCTACCAGCGTACCAATCATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGTCTGCCAGccaaactataacg                                                                                  <  1:1966546/100‑8 (MQ=38)
                   acagGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTg                                                                                  <  2:908831/100‑1 (MQ=255)
                    cagGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGt                                                                                 >  1:2128372/1‑100 (MQ=255)
                     agGAATGCCACTGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTc                                                                                <  1:2797002/100‑1 (MQ=255)
                      ggAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCa                                                                               >  1:556094/1‑100 (MQ=255)
                      ggAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCa                                                                               >  2:3295086/1‑100 (MQ=255)
                      ggAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCa                                                                               >  2:2340754/1‑100 (MQ=255)
                       gAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAg                                                                              >  1:3115682/1‑100 (MQ=255)
                        aaTGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAg                                                                              <  2:1537629/99‑1 (MQ=255)
                          tGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGCAGACAAACAAACTTGTCAGCAAg                                                                          >  1:2718554/1‑100 (MQ=255)
                           gCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAg                                                                          >  2:3970485/1‑100 (MQ=255)
                            ccAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGa                                                                         >  1:2700539/1‑100 (MQ=255)
                            ccAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGCGGCGTTATAGTTTGGCTCGCAGACAAACAAACTTGTCAGCAAGa                                                                         >  2:1370132/1‑100 (MQ=255)
                             cAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGAt                                                                        <  1:4049464/100‑1 (MQ=255)
                             cAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGAt                                                                        >  2:2221177/1‑100 (MQ=255)
                             cAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGAt                                                                        >  1:3162436/1‑100 (MQ=255)
                              aaTGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATc                                                                       >  2:1590741/1‑100 (MQ=255)
                              aaTGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGAt                                                                        >  1:2306237/1‑99 (MQ=255)
                               aTGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATcc                                                                      <  1:3933296/100‑1 (MQ=255)
                                tGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCa                                                                     <  1:1069451/100‑1 (MQ=255)
                                  gCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAACATCCAAt                                                                   >  1:2295513/1‑100 (MQ=255)
                                  gCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAAt                                                                   <  1:3343881/100‑1 (MQ=255)
                                   cGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATa                                                                  <  2:2295519/100‑1 (MQ=255)
                                     aTACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAACCTTGTCAGCAAGATCCAATAcc                                                                <  1:2480272/100‑1 (MQ=255)
                                          taccAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGa                                                           >  1:1389049/1‑100 (MQ=255)
                                            ccAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATg                                                         <  1:3019030/100‑1 (MQ=255)
                                            ccAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATg                                                         <  2:3052438/100‑1 (MQ=255)
                                            ccAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATg                                                         <  2:1451395/100‑1 (MQ=255)
                                             cAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGc                                                        >  1:1726504/1‑100 (MQ=255)
                                              aGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGATGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCa                                                       >  1:4257144/1‑100 (MQ=255)
                                               gCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCAt                                                      >  2:813097/1‑100 (MQ=255)
                                                    ccAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCgg                                                 >  2:3622295/1‑100 (MQ=255)
                                                     cAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGt                                                <  2:2778586/100‑1 (MQ=255)
                                                     cAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGt                                                <  2:3407231/100‑1 (MQ=255)
                                                     cAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGt                                                <  2:3833065/100‑1 (MQ=255)
                                                     cAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGt                                                >  2:3202608/1‑100 (MQ=255)
                                                       ataatCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTa                                              <  2:3754990/100‑1 (MQ=255)
                                                          atCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCt                                           <  2:4036668/100‑1 (MQ=255)
                                                            ccTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGa                                         >  2:1981117/1‑100 (MQ=255)
                                                            ccTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGa                                         >  1:832004/1‑100 (MQ=255)
                                                             cTTAACTTCAGGCGGTGGCGCGTGGCGTTGTAGTTTGGTTGGCGGACAAACAACCTTGTCAGCAAGATCCAATACCCGTGATGCAGTCCGGTTATCTGaa                                        >  1:1067629/1‑100 (MQ=255)
                                                             cTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGaa                                        <  2:2938334/100‑1 (MQ=255)
                                                             cTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGaa                                        <  2:1866792/100‑1 (MQ=255)
                                                              ttAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAat                                       <  1:44616/100‑1 (MQ=255)
                                                                 aCTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAatgat                                    >  1:625937/1‑100 (MQ=255)
                                                                   ttCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATgg                                  <  2:1726509/100‑1 (MQ=255)
                                                                   ttCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATgg                                  <  2:2631719/100‑1 (MQ=255)
                                                                   ttCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATgg                                  <  1:3526325/100‑1 (MQ=255)
                                                                   ttCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATgg                                  >  1:249756/1‑100 (MQ=255)
                                                                    tCAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGc                                 <  2:667549/100‑1 (MQ=255)
                                                                     cAGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCg                                <  2:4174785/100‑1 (MQ=255)
                                                                      aGGCGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGt                               >  2:4229777/1‑100 (MQ=255)
                                                                       ggCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTa                              <  1:1274927/100‑1 (MQ=255)
                                                                         cGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGc                            <  2:1249691/100‑1 (MQ=255)
                                                                         cGGTGGCGCGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGc                            <  2:3947782/100‑1 (MQ=255)
                                                                          ggTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGcc                           <  1:1929440/100‑1 (MQ=255)
                                                                              gcgcGTGGCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAg                       <  2:2096547/100‑1 (MQ=255)
                                                                               cgcgTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGc                      <  2:3550094/100‑1 (MQ=255)
                                                                                 cgTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGcac                    <  1:3192415/100‑1 (MQ=255)
                                                                                     gCGTTATAGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCacaaca                <  1:757939/100‑1 (MQ=255)
                                                                                     gCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCacaaca                >  1:2211962/1‑100 (MQ=255)
                                                                                      cGTTATAGTTTGGTTGGCAGACAAACAAACTCGTCAGCAAGATCCAATACCCGTGATGCATTCCGGATATCTGAATGATAGCGTAGCCGAAGCACAacac               >  1:856277/1‑100 (MQ=255)
                                                                                      cGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAacac               >  2:1349430/1‑100 (MQ=255)
                                                                                      cGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAacac               >  2:3352122/1‑100 (MQ=255)
                                                                                      cGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAacac               >  2:1139882/1‑100 (MQ=255)
                                                                                       gTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCAGAAGCACAacac               >  1:897476/1‑99 (MQ=255)
                                                                                         tataGTTTGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAg            <  2:3448914/100‑1 (MQ=255)
                                                                                         tataGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAg            <  2:264349/100‑1 (MQ=255)
                                                                                         tataGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAg            >  2:4139246/1‑100 (MQ=255)
                                                                                           taGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGTATTCAGGTTATCTGAATGATGGCGTAGCCGAAGCACAGCACAAg            >  2:505470/1‑98 (MQ=255)
                                                                                           taGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCg          <  2:3380304/100‑1 (MQ=255)
                                                                                             gTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCGAc        >  2:1236538/1‑100 (MQ=255)
                                                                                             gTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCGAc        <  1:2340748/100‑1 (MQ=255)
                                                                                             gTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCGAc        >  1:1097570/1‑100 (MQ=255)
                                                                                              tttGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCGCAACACCAGCGACa       >  1:2912403/1‑100 (MQ=255)
                                                                                              tttGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCGACa       >  2:870156/1‑100 (MQ=255)
                                                                                              tttGGTTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCGACa       >  2:976050/1‑100 (MQ=255)
                                                                                                  gctggcAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCGACATTCt   >  2:1175204/1‑100 (MQ=255)
                                                                                                   ctggcAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCGACATTCt   <  1:3702565/99‑1 (MQ=255)
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TGATGGCACAAACCACAGCACAGGAATGCCAATGGCGATACCTACCAGCGTACCAATAATCCTTAACTTCAGGCGGTGGCGCGTGGCGTTATAGTTTGGCTGGCAGACAAACAAACTTGTCAGCAAGATCCAATACCCGTGATGCATTCCGGTTATCTGAATGATGGCGTAGCCGAAGCACAACACCAGCGACATTCTT  >  NC_000913/1022501‑1022699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: