Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,023,771 A→T 47.6% V38E (GTA→GAA)  yccS ← putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,023,7710AT47.6% 31.7 / 216.7 151V38E (GTA→GAA) yccSputative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (37/42);  new base T (32/40);  total (69/82)
Fisher's exact test for biased strand distribution p-value = 8.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

AGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGTTTGAGC  >  NC_000913/1023672‑1023869
                                                                                                   |                                                                                                  
aGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTa                                                                                                    <  1:1187341/100‑1 (MQ=255)
  cGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACa                                                                                                  <  2:129043/100‑1 (MQ=255)
   gTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACAt                                                                                                 >  2:1093724/1‑100 (MQ=255)
     aaTGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCa                                                                                               <  1:4135937/100‑1 (MQ=255)
         atgaGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCa                                                                                           <  1:942096/100‑1 (MQ=255)
          tgaGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAg                                                                                          <  2:2415256/100‑1 (MQ=255)
           gaGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCGGCATCCCCAGAGTTAGCGGAATCGTCAGATATACATCAccctg                                                                                          >  1:2147496/1‑97 (MQ=255)
             ggTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGcca                                                                                       >  2:1044093/1‑100 (MQ=255)
             cattACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGcca                                                                                       <  2:3357320/98‑1 (MQ=255)
                tACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGCAATCGTCAGTTTTACATCACCCAGccacca                                                                                    <  2:1104792/100‑1 (MQ=255)
                  cGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACg                                                                                  >  1:1412830/1‑100 (MQ=255)
                  cGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACg                                                                                  >  2:4031197/1‑100 (MQ=255)
                  cGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACg                                                                                  <  1:2388592/100‑1 (MQ=255)
                  cGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGACGCTGCCACCATCCCCAGCGTTAGCCGAAGAGTCAGTTTTTCATCACCCAGcc                                                                                        >  1:2062306/1‑94 (MQ=255)
                  cGCAAACGTACCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACg                                                                                  >  2:554933/1‑100 (MQ=255)
                    cAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGa                                                                                >  1:1903946/1‑100 (MQ=255)
                        cGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACg                                                                            >  2:890083/1‑100 (MQ=255)
                           cccGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCATCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCtg                                                                         <  2:4250328/100‑1 (MQ=255)
                           cccGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCtg                                                                         <  1:447439/100‑1 (MQ=255)
                           cccGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCtg                                                                         >  2:2353600/1‑100 (MQ=255)
                            ccGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCtgt                                                                        >  1:1123673/1‑100 (MQ=255)
                              gCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCTGTTTTACATCACCCAGCCACCACGGAAACGCTGTgg                                                                      <  2:3013767/100‑1 (MQ=255)
                                cAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGtt                                                                    >  1:561628/1‑100 (MQ=255)
                                  gTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCCCCCAGCCACCACGGAAACGCTGTGGTTcc                                                                  <  1:3861809/100‑1 (MQ=255)
                                    cGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCcac                                                                <  1:1148374/100‑1 (MQ=255)
                                    cGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCcac                                                                <  2:3727702/100‑1 (MQ=255)
                                     ggTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCcaca                                                               <  2:82007/100‑1 (MQ=255)
                                     ggTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCcaca                                                               >  1:2364682/1‑100 (MQ=255)
                                     ggTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCcaca                                                               <  2:2419397/100‑1 (MQ=255)
                                     ggTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCcaca                                                               <  2:1975845/100‑1 (MQ=255)
                                        cATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAg                                                            <  2:4274869/100‑1 (MQ=255)
                                        cATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAg                                                            >  2:217865/1‑100 (MQ=255)
                                         aTCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAgc                                                           <  1:2681926/100‑1 (MQ=255)
                                         aTCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAgc                                                           <  2:2348537/100‑1 (MQ=255)
                                          tCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAgcg                                                          >  2:29578/1‑100 (MQ=255)
                                            gagaTCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCa                                                        >  2:3581221/1‑100 (MQ=255)
                                            gagaTCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCa                                                        <  1:4188521/100‑1 (MQ=255)
                                             agaTCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCaa                                                       <  1:468996/100‑1 (MQ=255)
                                              gaTCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAAt                                                      >  1:547087/1‑100 (MQ=255)
                                              gaTCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAAt                                                      >  1:4044731/1‑100 (MQ=255)
                                                 cGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTATTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaa                                                   <  1:3649858/100‑1 (MQ=255)
                                                  ggTCAGCGCCGCTGCCACGATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGGCATCACGGAAACGCTGTGGTTCCACAACGCGCAATaaaa                                                  >  1:994463/1‑100 (MQ=255)
                                                  ggTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaa                                                  >  1:736302/1‑100 (MQ=255)
                                                  ggTCAGCGCCGCTGCCACCATCCCCAGCGCTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaa                                                  >  1:1399457/1‑100 (MQ=255)
                                                   gTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaaa                                                 >  2:1664001/1‑100 (MQ=255)
                                                   gTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaaa                                                 >  2:2060726/1‑100 (MQ=255)
                                                     cAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATa                                               <  1:864227/100‑1 (MQ=255)
                                                     cAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATa                                               <  1:875677/100‑1 (MQ=255)
                                                     cAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATa                                               <  1:2513197/100‑1 (MQ=255)
                                                      aGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAc                                              <  2:2756625/100‑1 (MQ=255)
                                                       gcgcCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcg                                             <  2:656518/100‑1 (MQ=255)
                                                         gccgcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcgcg                                           <  1:1938059/100‑1 (MQ=255)
                                                          ccgcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcgcgc                                          <  2:3037148/100‑1 (MQ=255)
                                                          ccgcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcgcg                                           <  1:3936436/99‑1 (MQ=255)
                                                           cgcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcgcgcg                                         >  2:4039652/1‑100 (MQ=255)
                                                            gcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGt                                        <  1:978704/100‑1 (MQ=255)
                                                            gcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGt                                        >  2:3243689/1‑100 (MQ=255)
                                                              tGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTaa                                      <  1:1044133/100‑1 (MQ=255)
                                                                ccaccaTCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATa                                    <  2:392413/100‑1 (MQ=255)
                                                                 caccaTCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATAc                                   <  2:1580919/100‑1 (MQ=255)
                                                                  accaTCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACa                                  >  2:507607/1‑100 (MQ=255)
                                                                  accaTCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACa                                  >  2:805735/1‑100 (MQ=255)
                                                                  accaTCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACa                                  >  1:2640224/1‑100 (MQ=255)
                                                                    caTCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGc                                <  2:2841156/100‑1 (MQ=255)
                                                                     aTCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGcc                               <  2:1395845/100‑1 (MQ=255)
                                                                       ccccAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAg                             >  1:1461976/1‑100 (MQ=255)
                                                                        cccAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAgg                            <  2:3709747/100‑1 (MQ=255)
                                                                        cccAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAgg                            >  1:3350499/1‑100 (MQ=255)
                                                                         ccAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGcgcctg                           <  1:1259090/100‑6 (MQ=255)
                                                                         ccAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGgc                           <  2:3882185/100‑1 (MQ=255)
                                                                          cAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGgcg                          <  2:2682104/100‑1 (MQ=255)
                                                                                tAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTc                    >  2:183347/1‑100 (MQ=255)
                                                                                 aGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTcc                   <  2:969396/100‑1 (MQ=255)
                                                                                  gCGGAATCGTCAGTTTTTCATCACCCAGCCACCCCGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTcc                   >  2:1551671/1‑99 (MQ=255)
                                                                                  gCGGAATCGTCAGTTTTACATCGCCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCa                  >  2:319079/1‑100 (MQ=255)
                                                                                  gCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCa                  >  2:1025981/1‑100 (MQ=255)
                                                                                  gCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCa                  >  2:3913110/1‑100 (MQ=255)
                                                                                    ggtaTCGTCAGCTTTTCATCACCCAGCGACGACTGAAACGCTGGGGATCCTCAAAACGCAATAAGAATGCGCGCGTAATACAGCCAggaact                        <  1:1648889/89‑5 (MQ=255)
                                                                                    ggAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAgg                <  1:1533060/100‑1 (MQ=255)
                                                                                    ggAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAgg                >  2:3636791/1‑100 (MQ=255)
                                                                                    ggAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAgg                <  1:2207151/100‑1 (MQ=255)
                                                                                    ggAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAgg                <  1:2442407/100‑1 (MQ=255)
                                                                                     gAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGt               <  2:3845474/100‑1 (MQ=255)
                                                                                     gAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGt               >  1:3160876/1‑100 (MQ=255)
                                                                                      aaTCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGta              <  1:3850103/100‑1 (MQ=255)
                                                                                       aTCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGtat             <  1:3058504/100‑1 (MQ=255)
                                                                                       aTCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGtat             <  1:703035/100‑1 (MQ=255)
                                                                                         cGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAg           <  1:2715711/100‑1 (MQ=255)
                                                                                         cGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAg           >  2:3525726/1‑100 (MQ=255)
                                                                                         cGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAg           >  2:2756245/1‑100 (MQ=255)
                                                                                          gTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGc          <  1:1044090/100‑1 (MQ=255)
                                                                                          gTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGc          >  1:2773965/1‑100 (MQ=255)
                                                                                          gTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGc          >  2:2857675/1‑100 (MQ=255)
                                                                                          gTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTCGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGc          >  1:2623311/1‑100 (MQ=255)
                                                                                             aGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGtt       >  1:2396829/1‑100 (MQ=255)
                                                                                             aGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGtt       >  1:1835258/1‑100 (MQ=255)
                                                                                             aGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGtt       <  2:1966161/100‑1 (MQ=255)
                                                                                              gTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGttt      >  1:337518/1‑100 (MQ=255)
                                                                                              gTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGttt      <  1:364221/100‑1 (MQ=255)
                                                                                                  tACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGTTTGAGc  >  2:2661963/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
AGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGTTTGAGC  >  NC_000913/1023672‑1023869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: