Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,070,115 C→A 31.0% P80P (CCG→CCT)  rutF ← flavin:NADH reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,070,1150CA31.0% 242.6 / 143.2 158P80P (CCG→CCT) rutFflavin:NADH reductase
Reads supporting (aligned to +/- strand):  ref base C (47/62);  new base A (29/20);  total (76/82)
Fisher's exact test for biased strand distribution p-value = 8.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 159 total aligned reads displayed.

GCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGT  >  NC_000913/1070017‑1070213
                                                                                                  |                                                                                                  
gCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCg                                                                                                   <  2:2253937/100‑1 (MQ=255)
 ccTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAgg                                                                                                  >  1:2305805/1‑100 (MQ=255)
  ctctTCCAGTTGCGGGCATCCGTTCACCCCCCTCTGCTAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGc                                                                                                 <  1:2876481/100‑1 (MQ=255)
  ctctTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGc                                                                                                 <  1:3288517/100‑1 (MQ=255)
   tctTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCt                                                                                                >  2:3372505/1‑100 (MQ=255)
    ctTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTc                                                                                               >  2:4042649/1‑100 (MQ=255)
    ctTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTc                                                                                               <  1:1076789/100‑1 (MQ=255)
    ctTCCAGTTGCGGGCATCCGGTCACCCCCCTCTGCCAGCGGGCGGCGGCAAAGCGGTGTGCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTc                                                                                               >  1:1880821/1‑100 (MQ=255)
     ttCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTcc                                                                                              >  2:1013244/1‑100 (MQ=255)
      tCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCt                                                                                             <  2:760140/100‑1 (MQ=255)
      tCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCt                                                                                             <  2:760979/100‑1 (MQ=255)
        cAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGc                                                                                           <  1:2391789/100‑1 (MQ=255)
        cAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGc                                                                                           >  2:1900939/1‑100 (MQ=255)
         aGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGcc                                                                                          <  1:1751653/100‑1 (MQ=255)
            tGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCgg                                                                                       >  1:3610994/1‑100 (MQ=255)
              cGGGCATCCGGTCACCCGCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTGGCCGCCAAAAAGGTTTGAAAGAGGCGCCTGCCCGgcg                                                                                     <  1:1471635/100‑1 (MQ=255)
               gggCATCCGGTCACCCCTGTCTGCCAGGGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGgcgc                                                                                    <  2:646439/100‑1 (MQ=255)
               gggCATCCGGTCACCCCCGTCTGCCAGTGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGgcgc                                                                                    >  2:48867/1‑100 (MQ=255)
               gggCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGgcgc                                                                                    <  2:385759/100‑1 (MQ=255)
               gggCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGgcgc                                                                                    <  2:4025411/100‑1 (MQ=255)
                ggCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCt                                                                                   >  2:3962966/1‑100 (MQ=255)
                  cATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTaa                                                                                 >  2:2544492/1‑100 (MQ=255)
                  cATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTa                                                                                  >  1:1818527/1‑99 (MQ=255)
                   aTCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAg                                                                                <  1:166268/100‑1 (MQ=255)
                    tCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAgt                                                                               >  2:1526001/1‑100 (MQ=255)
                    tCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAgt                                                                               >  1:44195/1‑100 (MQ=255)
                     ccGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAgtg                                                                              >  1:2231164/1‑100 (MQ=255)
                     ccGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAgtg                                                                              <  1:301023/100‑1 (MQ=255)
                     ccGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAgtg                                                                              >  2:2755792/1‑100 (MQ=255)
                       ggTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTa                                                                            >  2:2791591/1‑100 (MQ=255)
                       ggTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTa                                                                            >  2:2171782/1‑100 (MQ=255)
                       ggTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTa                                                                            <  2:767189/100‑1 (MQ=255)
                           aCCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTa                                                                        <  1:1403147/100‑1 (MQ=255)
                            cccccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTac                                                                       <  2:1166740/100‑1 (MQ=255)
                            cccccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTac                                                                       <  2:684143/100‑1 (MQ=255)
                            cccccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTac                                                                       >  2:1322759/1‑100 (MQ=255)
                             ccccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTaca                                                                      <  2:56191/100‑1 (MQ=255)
                              cccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTacac                                                                     >  2:2361734/1‑100 (MQ=255)
                              cccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTacac                                                                     >  2:1575948/1‑100 (MQ=255)
                                cGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTacacac                                                                   >  2:2053142/1‑100 (MQ=255)
                                cGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTacacac                                                                   >  2:1522198/1‑100 (MQ=255)
                                  tCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAg                                                                 >  1:3962815/1‑100 (MQ=255)
                                  tCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAg                                                                 >  2:1082864/1‑100 (MQ=255)
                                     gCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGt                                                              >  2:610407/1‑100 (MQ=255)
                                     gCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGt                                                              <  2:281380/100‑1 (MQ=255)
                                      ccAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGtt                                                             <  1:3045394/100‑1 (MQ=255)
                                       cAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTc                                                            >  1:3167397/1‑100 (MQ=255)
                                       cAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTc                                                            >  2:2090629/1‑100 (MQ=255)
                                        aGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCg                                                           >  2:3785875/1‑100 (MQ=255)
                                         gcgggcggCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGa                                                          <  2:3949464/100‑1 (MQ=255)
                                         gcgggcggCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGa                                                          <  2:4052553/100‑1 (MQ=255)
                                          cggggggCGGCAAAGCGGTGTTCCGTGGGAATATAGAAGAAAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGa                                                          <  1:3752939/99‑1 (MQ=255)
                                           gggcggCGGCAAAGCGGTGTTCCCTGGGCGTTTTGCCGCCAAAACGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGAt                                                         <  1:1262626/99‑1 (MQ=255)
                                            ggcggcggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGAttt                                                       >  1:521002/1‑100 (MQ=255)
                                               ggcggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCa                                                    <  2:1010991/100‑1 (MQ=255)
                                                 cggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCAtt                                                  >  1:2228382/1‑100 (MQ=255)
                                                 cggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCAtt                                                  <  1:867161/100‑1 (MQ=255)
                                                  ggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTg                                                 <  1:2694550/100‑1 (MQ=255)
                                                   gcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGa                                                >  2:716513/1‑100 (MQ=255)
                                                   gcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGa                                                <  1:2897663/100‑1 (MQ=255)
                                                    cAAAGCGGTGTTCCATGGGCGATTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACAGACAGCGTTCGATTCTCATTGaa                                               >  1:2551438/1‑100 (MQ=255)
                                                     aaaGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAAt                                              <  2:3992563/100‑1 (MQ=255)
                                                      aaGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATg                                             <  2:653125/100‑1 (MQ=255)
                                                      aaGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATg                                             <  1:4171601/100‑1 (MQ=255)
                                                          ggTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCgg                                         <  2:3079521/100‑1 (MQ=255)
                                                            tgtTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGcc                                       <  2:855364/100‑1 (MQ=255)
                                                            tgtTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGcc                                       <  2:3609248/100‑1 (MQ=255)
                                                             gtTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCa                                      >  2:2004199/1‑100 (MQ=255)
                                                                 cATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAgacg                                  <  2:3110461/100‑1 (MQ=255)
                                                                    gggCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAgacggac                               <  2:3739770/100‑1 (MQ=255)
                                                                       cGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGcc                            <  1:652661/100‑1 (MQ=255)
                                                                        gTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGcc                            <  2:3560858/99‑1 (MQ=255)
                                                                         ttttGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGcccc                          >  2:2919727/1‑100 (MQ=255)
                                                                            tGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACg                       >  2:4123148/1‑100 (MQ=255)
                                                                            tGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACg                       >  1:724546/1‑100 (MQ=255)
                                                                             gccgccAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGa                      >  1:1765811/1‑100 (MQ=255)
                                                                             gccgccAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGa                      >  1:3294310/1‑100 (MQ=255)
                                                                             gccgccAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGa                      >  1:2440393/1‑100 (MQ=255)
                                                                             gccgccAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGa                      >  2:2517907/1‑100 (MQ=255)
                                                                               cgccAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGAtt                    >  2:2169129/1‑100 (MQ=255)
                                                                                   aaaaaGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAgg                <  1:610405/100‑1 (MQ=255)
                                                                                   aaaaaGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAgg                >  2:1308528/1‑100 (MQ=255)
                                                                                   aaaaaGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAgg                <  2:397495/100‑1 (MQ=255)
                                                                                   aaaaaGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTAGATTGTCATTGAATGCCGTCCAGACGGACGCCCCACGATTCAg                 >  1:1366708/1‑99 (MQ=255)
                                                                                    aaatGGTTTGAAAGCGGCTCTTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGc               <  1:602483/100‑1 (MQ=255)
                                                                                    aaaaGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGc               <  1:228591/100‑1 (MQ=255)
                                                                                    aaaaGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGc               <  1:2845433/100‑1 (MQ=255)
                                                                                      aaGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGcac             >  2:316086/1‑100 (MQ=255)
                                                                                      aaGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGcac             <  1:3643139/100‑1 (MQ=255)
                                                                                        ggTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCacac           >  1:1990786/1‑100 (MQ=255)
                                                                                         gTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACAcc          <  1:2361728/100‑1 (MQ=255)
                                                                                         gTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACAcc          <  1:157049/100‑1 (MQ=255)
                                                                                         gTTTGAAAGAGGCTCCTGCCCGGTGCTAAGTGTATTTACACCCAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACAcc          >  1:1072277/1‑100 (MQ=255)
                                                                                         gTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACAcc          >  1:3776514/1‑100 (MQ=255)
                                                                                           ttGAAAGCGGCTCCTGCCCGGCGCTAAGTGAATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAg        <  1:4042629/100‑1 (MQ=255)
                                                                                           ttGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAg        >  2:2362118/1‑100 (MQ=255)
                                                                                             gAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTa      >  2:776509/1‑100 (MQ=255)
                                                                                              aaaGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTaa     >  1:128108/1‑100 (MQ=255)
                                                                                               aaGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAAt    >  1:3770851/1‑100 (MQ=255)
                                                                                               aaGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAAt    >  2:1072003/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
GCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGT  >  NC_000913/1070017‑1070213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑