Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,075,612 C→T 54.1% E1091K (GAA→AAA)  putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,075,6120CT54.1% 22.6 / 195.8 147E1091K (GAA→AAA) putAfused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (33/34);  new base T (43/36);  total (77/70)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

Only 100 of 162 total aligned reads displayed.

GCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTC  >  NC_000913/1075513‑1075711
                                                                                                   |                                                                                                   
 cgcgAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTcg                                                                                                    >  1:617241/1‑100 (MQ=255)
 cgcgAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTcg                                                                                                    <  2:1505151/100‑1 (MQ=255)
 cgcgAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTcg                                                                                                    <  2:2311078/100‑1 (MQ=255)
 cgcgAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGAAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTcg                                                                                                    >  2:1562221/1‑100 (MQ=255)
  gcgAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTcgc                                                                                                   <  2:2158408/100‑1 (MQ=255)
  acgAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTcgc                                                                                                   >  1:3234113/2‑100 (MQ=255)
   cgAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTcgcc                                                                                                  <  2:1568443/100‑1 (MQ=255)
     cgCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTtgccgc                                                                                                <  1:601490/99‑1 (MQ=255)
       cGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGccgccg                                                                                              <  2:829439/100‑1 (MQ=255)
       cGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGccgccg                                                                                              <  2:3062010/100‑1 (MQ=255)
        gTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGa                                                                                             >  1:1761270/1‑100 (MQ=255)
         tCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGaa                                                                                            <  2:1193408/100‑1 (MQ=255)
             tGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGGGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGc                                                                                        <  1:1708875/100‑1 (MQ=255)
             tGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGc                                                                                        >  2:3023635/1‑100 (MQ=255)
              gCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCt                                                                                       <  2:4225484/100‑1 (MQ=255)
              gCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCt                                                                                       >  1:745267/1‑100 (MQ=255)
                cAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGc                                                                                     <  2:3785647/100‑1 (MQ=255)
                  gcgcACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGcac                                                                                   <  1:3611142/100‑1 (MQ=255)
                   cgcACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGcaca                                                                                  <  1:3197578/100‑1 (MQ=255)
                    gcACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGCCAACCCTTTGCCGCCGAACGGCTGc                                                                                     >  2:4035548/1‑96 (MQ=255)
                    gcACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGcattt                                                                                 >  2:3389313/1‑97 (MQ=255)
                       cTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACaccaa                                                                              >  2:931238/1‑100 (MQ=255)
                         ttCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACaccaacc                                                                            <  2:1032478/100‑1 (MQ=255)
                           ccGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCAc                                                                          >  2:1139618/1‑100 (MQ=255)
                           ccGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCAc                                                                          <  1:3713758/100‑1 (MQ=255)
                            cGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACt                                                                         <  2:136982/100‑1 (MQ=255)
                              ggCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTgc                                                                       >  2:342335/1‑100 (MQ=255)
                               gCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTgcg                                                                      <  2:3529011/100‑1 (MQ=255)
                               gCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTgcg                                                                      >  2:4263836/1‑100 (MQ=255)
                                cGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTgcgc                                                                     >  2:2836520/1‑100 (MQ=255)
                                 gATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCCTTCGGCCCGGTACCGGACAACCCTTTGCCCCCGAACGGCTGCACACCAACCACTGCGcc                                                                    >  2:1073571/1‑100 (MQ=255)
                                     cGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCccacc                                                                >  1:769929/1‑100 (MQ=255)
                                     cGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCccacc                                                                <  2:2349149/100‑1 (MQ=255)
                                      gCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCccacca                                                               >  1:2708124/1‑100 (MQ=255)
                                      gCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAGCCACGGCGCccacct                                                               <  1:4053314/100‑2 (MQ=255)
                                      gCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCccacca                                                               >  2:2910441/1‑100 (MQ=255)
                                       ccAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTCCGCCCACCat                                                              >  2:3126083/1‑100 (MQ=255)
                                        cagcagACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCata                                                             >  1:646246/1‑100 (MQ=255)
                                          gcagACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATAtt                                                           >  1:1133412/1‑100 (MQ=255)
                                           cagACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTa                                                          >  2:2665574/1‑100 (MQ=255)
                                           cagACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTa                                                          <  2:3573536/100‑1 (MQ=255)
                                            agACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTAc                                                         >  1:2533960/1‑100 (MQ=255)
                                             gACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACg                                                        >  1:2068092/1‑100 (MQ=255)
                                               cGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGt                                                      >  2:2027290/1‑100 (MQ=255)
                                                ggTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGtt                                                     >  2:1000195/1‑100 (MQ=255)
                                                ggTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGtt                                                     <  2:2679859/100‑1 (MQ=255)
                                                  tAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTaa                                                   >  2:2167369/1‑100 (MQ=255)
                                                  tAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTaa                                                   <  2:3108683/100‑1 (MQ=255)
                                                      gaTAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAAACACTGCGCCCACCATATTACGGTTAACATa                                               >  1:632889/1‑100 (MQ=255)
                                                      gaTAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATa                                               <  1:2926827/100‑1 (MQ=255)
                                                       aTAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATAc                                              >  2:4177680/1‑100 (MQ=255)
                                                         agagCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAg                                            >  1:1317301/1‑100 (MQ=255)
                                                         agagCGGACCGCCTGCTTTCGGCCAGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCCCTGCCCCCACCATATTACGGCTAACATACa                                             >  1:3284603/1‑99 (MQ=255)
                                                          gagCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAgg                                           >  1:2692637/1‑100 (MQ=255)
                                                          gagCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAgg                                           <  2:3650491/100‑1 (MQ=255)
                                                          gagCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAgg                                           >  1:4214485/1‑100 (MQ=255)
                                                          gagCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAgg                                           <  2:862452/100‑1 (MQ=255)
                                                          gagCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGCTTAACATACAgg                                           <  2:2813105/100‑1 (MQ=255)
                                                            gCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCCCCATATTACGGTTACCATACAGGtt                                         >  1:1761431/1‑100 (MQ=255)
                                                            gCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGt                                          >  1:3143162/1‑99 (MQ=255)
                                                              ggACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTAc                                       <  2:3484119/100‑1 (MQ=255)
                                                               gACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTAcc                                      <  2:1851081/100‑1 (MQ=255)
                                                               gACCGCCTGCTTTCCGCCCAGTACCCGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAgg                                           <  1:4131214/95‑1 (MQ=255)
                                                                aCCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCa                                     <  2:3891680/100‑1 (MQ=255)
                                                                  cGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAAc                                   <  2:1969260/100‑1 (MQ=255)
                                                                   gCCTGCTTTCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACa                                  <  2:170920/100‑1 (MQ=255)
                                                                      tGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATgg                               <  2:4140559/100‑1 (MQ=255)
                                                                      tGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATgg                               <  2:212472/100‑1 (MQ=255)
                                                                       gCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATg                                >  1:1021018/1‑98 (MQ=255)
                                                                        ctgtCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACGCCAACCACTGCGCACACCATATTACGGTTAACATACAGGTTACCAACATGGGc                             <  1:2960846/97‑1 (MQ=255)
                                                                        cTTTCGGCCCGGTACCGGACAAACCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGc                             >  1:2392349/1‑100 (MQ=255)
                                                                         tttCGGCCCGGTACCGGACAACCCTTTGCCTCTGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGcc                            <  1:3466993/100‑1 (MQ=255)
                                                                         tttCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGcc                            >  2:590411/1‑100 (MQ=255)
                                                                         tttCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGcc                            <  2:646248/100‑1 (MQ=255)
                                                                         tttCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGcc                            <  2:1317305/100‑1 (MQ=255)
                                                                          ttCGGCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTACGCCCACCAGATTACGGTTAACATACAGGTTACCAACGTGGGc                             >  1:1897800/1‑98 (MQ=255)
                                                                            cGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCCGCACACCACCCACAGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGa                          <  1:1848064/99‑1 (MQ=255)
                                                                             ggCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGc                        >  2:22151/1‑100 (MQ=255)
                                                                             ggCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGc                        <  1:2511578/100‑1 (MQ=255)
                                                                              gCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGcc                       <  1:3431822/100‑1 (MQ=255)
                                                                              gCCCGGTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGcc                       <  1:2123733/100‑1 (MQ=255)
                                                                              gCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGcc                       >  1:2658282/1‑100 (MQ=255)
                                                                                ccGGTACCGGACAACCCTTTGCCGCCTAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAg                     <  2:3929966/100‑1 (MQ=255)
                                                                                ccGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAg                     <  1:3049499/100‑1 (MQ=255)
                                                                                ccGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAg                     <  1:4097840/100‑1 (MQ=255)
                                                                                  ggTACCGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTg                   >  1:1811141/1‑100 (MQ=255)
                                                                                    tACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGAc                 >  1:1444004/1‑100 (MQ=255)
                                                                                       cGGACACCCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTg              <  2:3993012/100‑1 (MQ=255)
                                                                                       cGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTg              >  2:252504/1‑100 (MQ=255)
                                                                                       cGGACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTg              >  2:19057/1‑100 (MQ=255)
                                                                                        ggACAACCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTgg             >  2:1663987/1‑100 (MQ=255)
                                                                                          atctcccTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGc           <  1:879644/96‑1 (MQ=255)
                                                                                             aCCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGAt        <  2:1197622/100‑1 (MQ=255)
                                                                                             aCCCTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTCACCAACAGGGGCCGAGCCAGGAACCTGGGCGa         >  1:819568/1‑99 (MQ=255)
                                                                                              cccTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATg       >  2:1758222/1‑100 (MQ=255)
                                                                                              cccTTCACCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTAACAACATGGGCCGAGCCAGTGACCTGGGCGATg       >  1:3183288/1‑100 (MQ=255)
                                                                                               ccTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATgg      >  1:1997670/1‑100 (MQ=255)
                                                                                               ccTTTGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATgg      >  1:2316267/1‑100 (MQ=255)
                                                                                                  tCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGtt    <  1:29910/99‑1 (MQ=255)
                                                                                                   cgccgcCGAGCGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTtc  <  1:4236848/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTC  >  NC_000913/1075513‑1075711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: