Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 194,506 A→T 35.7% Q329L (CAG→CTG)  dxr → 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913194,5060AT35.7% 168.3 / 179.3 171Q329L (CAG→CTG) dxr1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase
Reads supporting (aligned to +/- strand):  ref base A (55/55);  new base T (43/18);  total (98/73)
Fisher's exact test for biased strand distribution p-value = 1.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

Only 100 of 173 total aligned reads displayed.

CGCTCGATTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAATTTAT  >  NC_000913/194407‑194604
                                                                                                   |                                                                                                  
cGCTCGATTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCa                                                                                                    >  2:2439216/1‑100 (MQ=255)
   tCGATTTTTGCAAACTAAGTGCGTTGACATTTGCTGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAGCAACGCCAGGc                                                                                                 <  1:2242741/100‑1 (MQ=255)
     gATTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAg                                                                                               >  2:1441768/1‑100 (MQ=255)
     gATTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAg                                                                                               <  1:1917883/100‑1 (MQ=255)
     gATTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAg                                                                                               >  2:4184541/1‑100 (MQ=255)
      aTTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGc                                                                                              <  2:3974052/100‑1 (MQ=255)
      aTTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGc                                                                                              >  2:178463/1‑100 (MQ=255)
          ttGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGcgacg                                                                                          >  2:4165477/1‑100 (MQ=255)
           tGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGcgacga                                                                                         <  1:1625377/100‑1 (MQ=255)
            gCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCgacgac                                                                                        >  1:262901/1‑100 (MQ=255)
             cAAACTAGGTGCGTTGACATTTGCCGCTCCGGATTATGATCGTTATCCATGCCTGAAGCTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGcgacgat                                                                                        <  1:3554807/99‑2 (MQ=255)
               aaCTAAGTGCGTTGACATTTGCCGCACCGGATTATGAACGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGc                                                                                     >  1:2287515/1‑100 (MQ=255)
                aCTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCa                                                                                    <  2:139096/100‑1 (MQ=255)
                 cTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCAt                                                                                   <  1:4120807/100‑1 (MQ=255)
                  tAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCAtt                                                                                  <  2:1735125/100‑1 (MQ=255)
                  tAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCAtt                                                                                  >  1:32854/1‑100 (MQ=255)
                   aaGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTg                                                                                 >  2:2137577/1‑100 (MQ=255)
                     gTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGaa                                                                               >  1:862677/1‑100 (MQ=255)
                      tGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAAt                                                                              >  2:1441873/1‑100 (MQ=255)
                        cGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGc                                                                            <  2:1235368/100‑1 (MQ=255)
                         gTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGcc                                                                           <  1:2330450/100‑1 (MQ=255)
                          ttGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCg                                                                          <  2:3518440/100‑1 (MQ=255)
                          ttGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCg                                                                          <  2:3073982/100‑1 (MQ=255)
                          ttGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCg                                                                          >  2:2845659/1‑100 (MQ=255)
                           tGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGc                                                                         <  2:286122/100‑1 (MQ=255)
                            gACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCa                                                                        <  2:4264921/100‑1 (MQ=255)
                            gACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCa                                                                        >  1:934934/1‑100 (MQ=255)
                               aTTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAAc                                                                     <  1:1645884/100‑1 (MQ=255)
                                tttGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACg                                                                    <  2:3657548/100‑1 (MQ=255)
                                  tGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGaa                                                                  >  1:506786/1‑100 (MQ=255)
                                   gCCGCACCGGATTATGATCGTTATCCATGCGTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGaaa                                                                 <  1:373744/100‑1 (MQ=255)
                                   gCCGCACCGGATTATGATCGTTATACATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGCCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGaaa                                                                 >  1:3303950/1‑100 (MQ=255)
                                    ccGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAAt                                                                >  2:1046270/1‑100 (MQ=255)
                                    ccGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAAt                                                                >  2:108057/1‑100 (MQ=255)
                                    ccGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAAt                                                                <  2:3289224/100‑1 (MQ=255)
                                      gCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCa                                                              >  2:1757673/1‑100 (MQ=255)
                                       cACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGATGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCAc                                                             <  1:1112445/100‑1 (MQ=255)
                                         ncGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGATAGCATTGAATGCCGCAAACGAAATCACCg                                                           >  1:2563806/2‑100 (MQ=255)
                                         ccGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCg                                                           >  2:3885138/1‑100 (MQ=255)
                                         ccGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCg                                                           <  1:259167/100‑1 (MQ=255)
                                           ggATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGtt                                                         <  1:624488/100‑1 (MQ=255)
                                             aTTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgc                                                       >  2:662164/1‑100 (MQ=255)
                                             aTTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgc                                                       <  1:1027941/100‑1 (MQ=255)
                                             aTTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgc                                                       <  1:3167484/100‑1 (MQ=255)
                                              ttATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgct                                                      >  2:4150004/1‑100 (MQ=255)
                                                aTGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgctgc                                                    >  2:1231955/1‑100 (MQ=255)
                                                aTGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgctgc                                                    >  2:3440296/1‑100 (MQ=255)
                                                aTGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTtgctgc                                                    <  2:4033559/100‑1 (MQ=255)
                                                 tGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTgctgct                                                   >  2:971392/1‑100 (MQ=255)
                                                 tGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTgctgct                                                   >  1:734212/1‑100 (MQ=255)
                                                 tGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTgctgct                                                   <  1:2671766/100‑1 (MQ=255)
                                                  gATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCtt                                                  >  2:10854/1‑100 (MQ=255)
                                                     cGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTc                                               <  1:1301013/100‑1 (MQ=255)
                                                      gTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCt                                              <  1:2830573/100‑1 (MQ=255)
                                                      gTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCt                                              <  2:216913/100‑1 (MQ=255)
                                                        tATCCATGCCTGAAACTTGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTg                                            <  1:1497145/100‑1 (MQ=255)
                                                        tATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTg                                            <  1:1144574/100‑1 (MQ=255)
                                                        tATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCACAAACGAAATCACCGTTGCTGCTTTTCTTg                                            <  2:2522522/100‑1 (MQ=255)
                                                         aTCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTgc                                           <  1:3970040/100‑1 (MQ=255)
                                                         aTCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTCTTCTTgc                                           >  1:1291700/1‑100 (MQ=255)
                                                          tCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTgcg                                          <  1:3863866/100‑1 (MQ=255)
                                                          tCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTgcg                                          <  2:163798/100‑1 (MQ=255)
                                                          tCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTgcg                                          <  2:3684759/100‑1 (MQ=255)
                                                           ccATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTgcgc                                         >  1:3496670/1‑100 (MQ=255)
                                                            cATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCCTTTCTTGCGca                                        >  1:179832/1‑100 (MQ=255)
                                                             aTGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaa                                       >  1:2984573/1‑100 (MQ=255)
                                                               gCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaaca                                     >  2:2737036/1‑100 (MQ=255)
                                                               gCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaaca                                     >  1:3789197/1‑100 (MQ=255)
                                                               gCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaaca                                     >  1:294775/1‑100 (MQ=255)
                                                               gCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaaca                                     <  2:2791908/100‑1 (MQ=255)
                                                               gCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaaca                                     <  2:3806411/100‑1 (MQ=255)
                                                                ccTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaacaa                                    >  1:1425359/1‑100 (MQ=255)
                                                                ccTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGcaacaa                                    >  1:1342308/1‑100 (MQ=255)
                                                                 cTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACaaa                                   <  1:110339/100‑1 (MQ=255)
                                                                      aCTGGCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGc                              >  2:579211/1‑100 (MQ=255)
                                                                       cTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCt                             >  1:3116023/1‑100 (MQ=255)
                                                                         ggCGATGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCttt                           >  1:1625463/1‑100 (MQ=255)
                                                                           ctaTGGAGGCGTTAGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTAc                         >  2:2878772/3‑100 (MQ=255)
                                                                             aTGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACgg                       <  1:907899/100‑1 (MQ=255)
                                                                             aTGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACgg                       >  2:1564879/1‑100 (MQ=255)
                                                                             aTGGAGGCGCTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACgg                       >  1:1262017/1‑100 (MQ=255)
                                                                              tGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGa                      <  1:2757701/100‑1 (MQ=255)
                                                                              tGGAGGCGTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGa                      >  2:2024083/1‑100 (MQ=255)
                                                                                 aGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTCATCTTGCGCAACAAATCCGCTTTACGGata                    >  2:185712/1‑99 (MQ=255)
                                                                                  ggCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATc                  <  2:2920250/100‑1 (MQ=255)
                                                                                     gTTCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCt               >  2:1648723/1‑100 (MQ=255)
                                                                                      ttCGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTCCGCAACAAATCCGCTTTACGGATATCGc                >  1:505285/1‑98 (MQ=255)
                                                                                       tCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGc             <  2:1588699/100‑1 (MQ=255)
                                                                                        cGAACAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCg                 >  1:2485144/1‑95 (MQ=255)
                                                                                        cGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAACTCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGc             >  1:2018828/1‑99 (MQ=255)
                                                                                        cGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGc             >  1:1061972/1‑99 (MQ=255)
                                                                                         gAACAAGGCCTGGCAGCGACGACCGCATTGAATGCCGCAAACGAAACCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCg            <  1:1848342/99‑1 (MQ=255)
                                                                                         gAACAAGGCCAGGCAGCGACGACAGCATTCAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGt           >  2:3207152/1‑100 (MQ=255)
                                                                                            cAAGGCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGa        >  1:2541022/1‑100 (MQ=255)
                                                                                              aGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAt      <  2:1356016/100‑1 (MQ=255)
                                                                                               ggCCTGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAtt     >  2:3901372/1‑100 (MQ=255)
                                                                                               ggCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAtt     >  1:3879956/1‑100 (MQ=255)
                                                                                               ggCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAtt     <  2:1023848/100‑1 (MQ=255)
                                                                                                gCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAAt      <  1:4042276/98‑1 (MQ=255)
                                                                                                  cAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAATTTAt  <  2:588928/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
CGCTCGATTTTTGCAAACTAAGTGCGTTGACATTTGCCGCACCGGATTATGATCGTTATCCATGCCTGAAACTGGCGATGGAGGCGTTCGAACAAGGCCAGGCAGCGACGACAGCATTGAATGCCGCAAACGAAATCACCGTTGCTGCTTTTCTTGCGCAACAAATCCGCTTTACGGATATCGCTGCGTTGAATTTAT  >  NC_000913/194407‑194604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: