Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,125,966 C→A 46.5% T135T (ACC→ACA rimJ → ribosomal‑protein‑S5‑alanine N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,125,9660CA46.5% 43.8 / 214.9 159T135T (ACC→ACArimJribosomal‑protein‑S5‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (36/49);  new base A (42/32);  total (78/81)
Fisher's exact test for biased strand distribution p-value = 8.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

Only 100 of 165 total aligned reads displayed.

TCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAAAGAAGGCTATGCGAAA  >  NC_000913/1125867‑1126065
                                                                                                   |                                                                                                   
tCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCAcc                                                                                                     >  2:874534/1‑100 (MQ=255)
 cATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCAcc                                                                                                     <  2:2442477/99‑1 (MQ=255)
  aTGCCTGCTATCTCGGTTATGCGATTTGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCa                                                                                                   >  1:2614252/1‑100 (MQ=255)
   tGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGcacaca                                                                                                   <  1:2304736/99‑1 (MQ=255)
       tGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACata                                                                                              >  2:548824/1‑100 (MQ=255)
        gCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACatat                                                                                             <  2:3234209/100‑1 (MQ=255)
        gCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACatat                                                                                             <  2:2937175/100‑1 (MQ=255)
          tATCTCGGTTATTCGATTGGGCAAAAATGGCAGCGCAAAGGACTTAGGTTTGAAGCCCCGACCGCAGCCATTCGTTATATTCAGCGTACACAACatat                                                                                             >  1:2688944/1‑98 (MQ=255)
           aTCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCa                                                                                          <  2:164057/100‑1 (MQ=255)
            tctcGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGCCCGCAGCCATTCGTTCTATGCAGCGCACCCAACATATTCa                                                                                          >  1:336068/1‑99 (MQ=255)
            tctcGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCAt                                                                                         <  2:2702040/100‑1 (MQ=255)
             ctcGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATc                                                                                        <  1:844808/100‑1 (MQ=255)
             ctcGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCa                                                                                          >  2:1571689/1‑98 (MQ=255)
              tcGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCg                                                                                       <  1:1169533/100‑1 (MQ=255)
              tcGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCg                                                                                       >  1:2623938/1‑100 (MQ=255)
              tcGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCg                                                                                       >  1:3676478/1‑100 (MQ=255)
               cGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGc                                                                                      <  2:836819/100‑1 (MQ=255)
                    aTTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCACTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTAt                                                                                 >  1:4032672/1‑100 (MQ=255)
                       cGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGc                                                                              <  2:4248574/100‑1 (MQ=255)
                       cGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGc                                                                              >  1:3739720/1‑100 (MQ=255)
                           tGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCACAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAAt                                                                          <  2:2493065/100‑1 (MQ=255)
                            gggCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAAtt                                                                         <  2:3670423/100‑1 (MQ=255)
                            gggCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAAtt                                                                         >  1:1253347/1‑100 (MQ=255)
                             ggCAAAAATGGCAGGGCAAAGGACTCATGTTGGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATta                                                                        >  1:4003936/1‑100 (MQ=255)
                              gCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATtat                                                                       >  2:32500/1‑100 (MQ=255)
                               cAAAAATGTCAGTGCAAAGGACTTATGTTTGAAGCCCTGAATGCAGCTATTTGTTATTTGCATTGTATCCAACATTTTCATTGCATTATGGCTa                                                                            <  1:1281788/94‑1 (MQ=255)
                               cAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATtata                                                                      >  1:426789/1‑100 (MQ=255)
                               cAAAAATGACAGGGCAAAGGACTCATGTTTGAAGCACTGACCGCAGCGATTCGTTATATGCAGCGCACCAAACATCTCTATCGAATTATGGCTACTtata                                                                      >  1:3951387/1‑100 (MQ=255)
                                   aaTGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTGATTATATGcc                                                                  >  2:3106374/1‑100 (MQ=255)
                                     tGGCAGGGCAAAGGACTCATGTTTGAAGCTCTGACCGCAGCCATTCGTTATATGCAGCGCACACAAGATATTCATCGCATTATGGCTAATTATATGCCGc                                                                <  1:2564296/100‑1 (MQ=255)
                                     tGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGc                                                                <  2:3691703/100‑1 (MQ=255)
                                      ggCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGca                                                               <  2:1141307/100‑1 (MQ=255)
                                      ggCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGca                                                               <  2:3356526/100‑1 (MQ=255)
                                      ggCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGc                                                                >  1:3504347/1‑99 (MQ=255)
                                       gCAGGGCAAAGGACTCATGTTTTAAGCCCTGATCGCAGCCATTAGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGcac                                                              <  1:1906085/100‑1 (MQ=255)
                                       gCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTCTGGCTAATTATATGCCGcac                                                              >  2:2104652/1‑100 (MQ=255)
                                       gCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGcac                                                              >  2:2105148/1‑100 (MQ=255)
                                        cAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGcaca                                                             >  1:2224654/1‑100 (MQ=255)
                                         aGGGCAAAGGACTCATTTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACaa                                                            <  1:1440376/100‑1 (MQ=255)
                                          gggCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAAt                                                           <  2:983945/100‑1 (MQ=255)
                                          gggCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAAt                                                           >  1:1913306/1‑100 (MQ=255)
                                             cAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATaaa                                                        >  1:743937/1‑100 (MQ=255)
                                                aGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGc                                                     <  2:1918728/100‑1 (MQ=255)
                                                aGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGc                                                     <  2:1918741/100‑1 (MQ=255)
                                                  gACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGcacagatcggaaga                                                   >  1:550220/1‑90 (MQ=255)
                                                  gACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAg                                                   >  2:271181/1‑100 (MQ=255)
                                                    cTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCGAATTATATGCCGCACAATAAACGCa                                                    >  1:1711297/1‑97 (MQ=255)
                                                    cTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGc                                                  <  1:1302803/99‑1 (MQ=255)
                                                     tCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCgg                                                <  1:3859389/100‑1 (MQ=255)
                                                      cATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGt                                               <  1:1538743/100‑1 (MQ=255)
                                                      cATGTTTGAAGCCCTGACCGCAACCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGt                                               >  1:284631/1‑100 (MQ=255)
                                                       aTGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCCGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCTCAATAAACGCAGCGGTg                                              <  1:4013458/100‑1 (MQ=255)
                                                        tGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGa                                             >  2:197565/1‑100 (MQ=255)
                                                        tGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCCTCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGa                                             >  1:1995175/1‑100 (MQ=255)
                                                        tGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGa                                             >  2:4116501/1‑100 (MQ=255)
                                                         gTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGAt                                            >  1:3037818/1‑100 (MQ=255)
                                                         gTTTGAAGCCCTGACCGCAGCCATTCATTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGAt                                            >  2:2597529/1‑100 (MQ=255)
                                                          tttGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGAACAATAAACTCAGCGGTGAt                                            <  1:2119564/99‑1 (MQ=255)
                                                          tttGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGAtt                                           <  2:1027981/100‑1 (MQ=255)
                                                          tttGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGAtt                                           >  1:680032/1‑100 (MQ=255)
                                                            tGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTa                                         >  1:1025041/1‑100 (MQ=255)
                                                             gAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTAc                                        >  2:3030507/1‑100 (MQ=255)
                                                             gAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTAc                                        <  1:3374429/100‑1 (MQ=255)
                                                             gAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTAc                                        >  2:3290049/1‑100 (MQ=255)
                                                                gCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTgg                                     <  2:1609769/100‑1 (MQ=255)
                                                                gCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTgg                                     <  1:1550556/100‑1 (MQ=255)
                                                                gCCCTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTgg                                     <  2:474187/100‑1 (MQ=255)
                                                                 cccTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGgc                                    <  2:1011554/100‑1 (MQ=255)
                                                                  ccTGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGgcg                                   <  1:1176740/100‑1 (MQ=255)
                                                                  catGACCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGgcg                                   <  1:3021235/98‑1 (MQ=255)
                                                                      aCCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGAc                               <  2:3338277/100‑1 (MQ=255)
                                                                      aCCGCAGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGAc                               >  1:654187/1‑100 (MQ=255)
                                                                         gCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGGGATTTACTGGCGCGACTgg                            >  1:1506587/1‑100 (MQ=255)
                                                                          cAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTggg                           <  2:1555323/100‑1 (MQ=255)
                                                                           aGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGt                          >  2:3475789/1‑100 (MQ=255)
                                                                           aGCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGt                          >  2:3933502/1‑100 (MQ=255)
                                                                            gCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTggg                           >  1:921640/1‑98 (MQ=255)
                                                                            gCCATTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTggg                           >  1:3415789/1‑98 (MQ=255)
                                                                             ccATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGttt                        <  1:4208616/100‑1 (MQ=255)
                                                                              cATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTCTATGCCGCCCAATAAACGCAGCGGTGATTTACTGGCGCGACTGCGttt                        >  1:1047454/1‑99 (MQ=255)
                                                                               aTTCGTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTg                      >  2:1372976/1‑100 (MQ=255)
                                                                                   gatataTGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGaaaa                  >  2:2897528/3‑100 (MQ=255)
                                                                                   gTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGaaaa                  >  1:1617579/1‑100 (MQ=255)
                                                                                   gTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGaaaa                  >  1:2830013/1‑100 (MQ=255)
                                                                                   gTTATATGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGaaaa                  <  2:1327844/100‑1 (MQ=255)
                                                                                    ttATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGaaaaa                 <  2:1847824/100‑1 (MQ=255)
                                                                                       tatGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAaagaa              <  2:370329/100‑1 (MQ=255)
                                                                                       tatGCAGCGCACACAACATATTCATCGCATTATAGATAATTATATGCAACACAATAAACGAAGCGTAGATGTACTGGCCGGACTGGGCTTTTaaaaggc               >  1:3533341/1‑96 (MQ=255)
                                                                                        atGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAaagaag             >  2:668903/1‑100 (MQ=255)
                                                                                        atGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAaagaag             >  2:3389510/1‑100 (MQ=255)
                                                                                        atGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAaagaag             <  1:286574/100‑1 (MQ=255)
                                                                                         tGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAaagaag             >  1:4043753/1‑99 (MQ=255)
                                                                                         tGCAGCGCACACAACATATTTATCGCATTATGGCGGATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAAAGAAgg            <  1:374782/100‑1 (MQ=255)
                                                                                         tGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAAAGAAgg            >  2:433414/1‑100 (MQ=255)
                                                                                         tGCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAAAGAAgg            >  1:3308716/1‑100 (MQ=255)
                                                                                          gCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAAAGAAGGc           <  1:1670550/100‑1 (MQ=255)
                                                                                          gCAGCGCACACAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAAAGAAGGc           >  1:715447/1‑100 (MQ=255)
                                                                                           cAGCGCACACAACATATTCATCGCATTATGGCGAAGTATATGCCGCACAATAAACGCAGCGGGGATTTACTGGCGCGACCGGGTTGTGAAAAAGAAGGCt          >  1:251719/1‑100 (MQ=255)
                                                                                                cACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAAAGAAGGCTATGCg     <  2:2629276/100‑1 (MQ=255)
                                                                                                   ccAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAAAGAAGGCTATGCGaaa  >  2:1342740/1‑100 (MQ=255)
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TCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCATGTTTGAAGCCCTGACCGCAGCCATTCGTTATATGCAGCGCACCCAACATATTCATCGCATTATGGCTAATTATATGCCGCACAATAAACGCAGCGGTGATTTACTGGCGCGACTGGGTTTTGAAAAAGAAGGCTATGCGAAA  >  NC_000913/1125867‑1126065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: