Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,128,973 G→A 23.0% D379N (GAC→AAC)  murJ → putative lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,128,9730GA23.0% 290.7 / 82.7 152D379N (GAC→AAC) murJputative lipid II flippase
Reads supporting (aligned to +/- strand):  ref base G (66/51);  new base A (18/17);  total (84/68)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 156 total aligned reads displayed.

GATGCGCTGATGACCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCAC  >  NC_000913/1128874‑1129072
                                                                                                   |                                                                                                   
 aTGCGCTGATGACCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGa                                                                                                    >  2:2220577/1‑100 (MQ=255)
 aTGCGCTGATGACCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGa                                                                                                    >  1:819466/1‑100 (MQ=255)
  tGCGCTGATGACCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGAc                                                                                                   <  2:333565/100‑1 (MQ=255)
  tGCGCTGATGACCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGAc                                                                                                   >  1:2425381/1‑100 (MQ=255)
     gcTGATGACCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCAGCCAGGACAtt                                                                                                >  1:4081196/1‑100 (MQ=255)
      cTGATGACCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTa                                                                                               <  2:3489937/100‑1 (MQ=255)
       tgatgaCCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGGTTGTAGTGAAAGTGTTGGCTCCTGGCTTTTCTTCCCGCCAGGACATTa                                                                                               >  1:1905983/1‑99 (MQ=255)
          tgaCCCAGCGGGCGTTAATTGCCTCCTCGGTGGGGTTGCTTGGCCTGCTCGTCGTGAAAGCGTTGGCTCCCGGCATTTATTCCCGCCCGGACATCaaaa                                                                                            >  1:1013146/1‑99 (MQ=255)
            aCCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGc                                                                                         >  1:2004040/1‑100 (MQ=255)
             cccAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGcc                                                                                        >  2:799716/1‑100 (MQ=255)
             cccAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGcc                                                                                        >  2:776902/1‑100 (MQ=255)
                aGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGt                                                                                     >  1:4104097/1‑100 (MQ=255)
                   gggCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGaa                                                                                  >  1:4108554/1‑100 (MQ=255)
                     gCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGaaaa                                                                                <  2:4040594/100‑1 (MQ=255)
                      cGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAAt                                                                               >  1:630555/1‑100 (MQ=255)
                         tgaTTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGc                                                                            <  2:1207963/98‑1 (MQ=255)
                         tAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTg                                                                             >  1:459616/1‑99 (MQ=255)
                         tAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGc                                                                            <  2:2920160/100‑1 (MQ=255)
                         tAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGc                                                                            <  1:3809371/100‑1 (MQ=255)
                          aaTTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGcc                                                                           <  1:1097068/100‑1 (MQ=255)
                             tGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATc                                                                        >  2:1438121/1‑100 (MQ=255)
                             tGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATc                                                                        >  2:578022/1‑100 (MQ=255)
                             tGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAATCGCCAGTGAAAATTGCCATc                                                                        <  1:2601575/100‑1 (MQ=255)
                               ccTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGt                                                                      <  1:1340863/100‑1 (MQ=255)
                                    tCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGc                                                                 >  1:1304108/1‑100 (MQ=255)
                                    tCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGc                                                                 <  2:1751107/100‑1 (MQ=255)
                                     cGGTGGGTTTGGTTGGACTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCt                                                                >  1:3134418/1‑100 (MQ=255)
                                     cGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCt                                                                <  1:639818/100‑1 (MQ=255)
                                        tGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGAt                                                             <  1:3085667/100‑1 (MQ=255)
                                        tGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGAt                                                             <  2:1637801/100‑1 (MQ=255)
                                          ggTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGAttt                                                           >  2:1096485/1‑100 (MQ=255)
                                          ggTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGAttt                                                           <  1:435561/100‑1 (MQ=255)
                                           gTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGAtttt                                                          >  1:271001/1‑100 (MQ=255)
                                           gTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGAtttt                                                          >  2:2779045/1‑100 (MQ=255)
                                           gTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGAtttt                                                          >  2:2082630/1‑100 (MQ=255)
                                           gTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGAtttt                                                          >  1:790550/1‑100 (MQ=255)
                                           gTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATGACTGCAAGAACATTAAAGAGACAGTGAAAATTGCCATCGTTACGCTGa                                                              >  1:1552221/1‑96 (MQ=255)
                                             ttGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTaa                                                        >  1:1688182/1‑100 (MQ=255)
                                              tGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCCGTGAAAATTGCCATCGTTACGCTGATTTTAAc                                                       >  1:1008558/1‑100 (MQ=255)
                                               gATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACg                                                      <  2:1240794/100‑1 (MQ=255)
                                                aTTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGc                                                     <  2:3955683/100‑1 (MQ=255)
                                                  tGGCCTGATTGTAGTGAAAGTGTGGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCAGCGTTACGCTGATTTTAACGCa                                                    <  1:3554820/99‑1 (MQ=255)
                                                   ggCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCaa                                                   >  2:2289834/1‑99 (MQ=255)
                                                   ggCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAAt                                                  >  1:2837085/1‑100 (MQ=255)
                                                    gCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCaa                                                   >  1:2964436/1‑98 (MQ=255)
                                                    gCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAAtt                                                 <  2:4081218/100‑1 (MQ=255)
                                                    gCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCaa                                                   >  1:406063/1‑98 (MQ=255)
                                                     ccTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATtg                                                >  1:2702946/1‑100 (MQ=255)
                                                       tGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATtgat                                              <  2:2530001/100‑1 (MQ=255)
                                                       tGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATtgat                                              <  2:10378/100‑1 (MQ=255)
                                                        ttccgatctGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATtgatg                                             <  1:2196363/92‑1 (MQ=255)
                                                         aTTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATtgatga                                            <  2:253435/100‑1 (MQ=255)
                                                           tttAGTGTAAGTGTTGGCTCCTTGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGaa                                           <  1:1276187/97‑1 (MQ=255)
                                                            gTAGTGAAAGTGTTGGCTCCTGGCGTTTATTCCAGCCAGGACATTAAAGCGGCCGTGACAATTGCCATCGTTACGCTCATCTTAACGCAATtgatg                                             >  1:3069864/1‑96 (MQ=255)
                                                             tAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCt                                        >  1:1878022/1‑100 (MQ=255)
                                                             tAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCt                                        >  1:2814886/1‑100 (MQ=255)
                                                              aGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTg                                       >  2:377096/1‑100 (MQ=255)
                                                              aGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTg                                       <  1:3818791/100‑1 (MQ=255)
                                                               gTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTgg                                      >  2:332385/1‑100 (MQ=255)
                                                               gTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTgg                                      >  1:1549418/1‑100 (MQ=255)
                                                               gTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTgg                                      >  2:82087/1‑100 (MQ=255)
                                                                tGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGc                                     <  2:3154185/100‑1 (MQ=255)
                                                                tGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGc                                     >  1:3700071/1‑100 (MQ=255)
                                                                 gAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATtgatgagatcggaa                                    >  2:2196369/1‑92 (MQ=255)
                                                                 gAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCg                                    >  2:1661384/1‑100 (MQ=255)
                                                                    aGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGttt                                 >  2:2562744/1‑100 (MQ=255)
                                                                       gtTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTAtt                              <  2:1264976/100‑1 (MQ=255)
                                                                       gtTGGCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTAtt                              >  2:2357433/1‑100 (MQ=255)
                                                                        ttGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTg                             <  2:1060192/100‑1 (MQ=255)
                                                                         tGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTgg                            >  1:604586/1‑100 (MQ=255)
                                                                          ggCTCCTGGCTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGt                           >  2:1945638/1‑100 (MQ=255)
                                                                            cTCCTGGCTTTTATTCCCGCTAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTcc                         >  2:1933151/1‑100 (MQ=255)
                                                                             tCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCg                        >  2:1286824/1‑100 (MQ=255)
                                                                                tGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAATATTGCCATCGTTACGCTGATTTCAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTg                     <  1:2281977/100‑1 (MQ=255)
                                                                                 ggCTTTTATTCCCGCCAGGACATTAAATCGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGa                    <  1:2274831/100‑1 (MQ=255)
                                                                                  gCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGaa                   <  2:1433760/100‑1 (MQ=255)
                                                                                  gCTGTTATTCCCGCTAGACCATTAAAACGCCAGTGAAAATTGGCATCTGTACTCTGAGTTGATCGCAATTGATCAACCTGGCGTTTATTGGTCCGTTGaa                   <  1:68676/100‑1 (MQ=255)
                                                                                   cTTTTATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGaaa                  >  2:1574332/1‑100 (MQ=255)
                                                                                    ttttATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAAc                 <  1:216558/100‑1 (MQ=255)
                                                                                    ttttATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAAc                 <  1:2198508/100‑1 (MQ=255)
                                                                                    ttttATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAAc                 <  2:2922642/100‑1 (MQ=255)
                                                                                    ttttATTCCCGCCAGAACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAAc                 >  2:2386136/1‑100 (MQ=255)
                                                                                       atctCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATg              <  1:3547502/97‑1 (MQ=255)
                                                                                        aTTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATg              >  2:1646928/1‑99 (MQ=255)
                                                                                         ttCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGcc            >  1:2508737/1‑100 (MQ=255)
                                                                                         ttCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGcc            >  2:1198648/1‑100 (MQ=255)
                                                                                          tCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCg           >  1:833178/1‑100 (MQ=255)
                                                                                          tCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCATTTATTGGTCCGTTGAAACATGCCg           >  2:1500267/1‑100 (MQ=255)
                                                                                           cccGCCAGGACATTAAATCGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCgg          <  1:3188310/100‑1 (MQ=255)
                                                                                           cccGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTGACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCgg          >  1:93605/1‑100 (MQ=255)
                                                                                           cccGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATgagat          >  2:3547515/1‑96 (MQ=255)
                                                                                           cccGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCgg          >  2:2128556/1‑100 (MQ=255)
                                                                                           cccGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCgg          <  2:604588/100‑1 (MQ=255)
                                                                                           cccGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCgg          <  1:328150/100‑1 (MQ=255)
                                                                                             cGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGc        >  2:1028575/1‑100 (MQ=255)
                                                                                              gCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGc        <  1:3383197/99‑1 (MQ=255)
                                                                                              gCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCt       <  2:3763905/100‑1 (MQ=255)
                                                                                                  ggACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCa   >  2:56864/1‑100 (MQ=255)
                                                                                                   gACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTGACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCAc  <  2:93605/100‑1 (MQ=255)
                                                                                                   gACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCAc  >  2:1691518/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GATGCGCTGATGACCCAGCGGGCGTTAATTGCCTACTCGGTGGGTTTGATTGGCCTGATTGTAGTGAAAGTGTTGGCTCCTGGCTTTTATTCCCGCCAGGACATTAAAACGCCAGTGAAAATTGCCATCGTTACGCTGATTTTAACGCAATTGATGAACCTGGCGTTTATTGGTCCGTTGAAACATGCCGGGCTGTCAC  >  NC_000913/1128874‑1129072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: