Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,143,125 C→T 100% D415N (GAC→AAC)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,143,1250CT98.6% 487.7 / ‑2.7 146D415N (GAC→AAC) rneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (68/75);  total (69/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

Only 100 of 157 total aligned reads displayed.

ATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCC  >  NC_000913/1143028‑1143221
                                                                                                 |                                                                                                
aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtac                                                                                                <  2:2430742/100‑1 (MQ=255)
aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAACGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtac                                                                                                >  1:107103/1‑100 (MQ=255)
   ggCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgca                                                                                             >  2:269703/1‑100 (MQ=255)
     cacaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAACGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgcac                                                                                            >  1:2332453/1‑99 (MQ=255)
      acaGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACgg                                                                                          >  2:3393287/1‑100 (MQ=255)
       caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGt                                                                                         >  2:4113244/1‑100 (MQ=255)
       caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGt                                                                                         <  1:2845272/100‑1 (MQ=255)
        aGGAACAATGGCGTGAACTTCCTGTGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTg                                                                                        <  2:1378248/100‑1 (MQ=255)
        aGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTg                                                                                        <  1:2366471/100‑1 (MQ=255)
         ggAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGc                                                                                       >  2:262300/1‑100 (MQ=255)
         ggAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGc                                                                                       >  2:3262851/1‑100 (MQ=255)
          gAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGcc                                                                                      <  2:1965537/100‑1 (MQ=255)
          gAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGcc                                                                                      <  1:2613698/100‑1 (MQ=255)
           aaCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCa                                                                                     >  1:3665351/1‑100 (MQ=255)
           aaCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCa                                                                                     >  2:2277981/1‑100 (MQ=255)
               aTGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAc                                                                                 >  1:1796764/1‑100 (MQ=255)
               aTGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAc                                                                                 >  1:1330836/1‑100 (MQ=255)
                tGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAcc                                                                                <  2:3611174/100‑1 (MQ=255)
                 ggCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCa                                                                               <  1:3329616/100‑1 (MQ=255)
                   cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGa                                                                             <  1:71637/100‑1 (MQ=255)
                    gTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaa                                                                            >  1:243166/1‑100 (MQ=255)
                     tGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaac                                                                           <  2:3037836/100‑1 (MQ=255)
                       aaCTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaacaa                                                                         <  2:3508867/100‑1 (MQ=255)
                         cTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAAcg                                                                       >  2:3318383/1‑100 (MQ=255)
                         cTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAAcg                                                                       >  1:1745707/1‑100 (MQ=255)
                            ccTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCgg                                                                    <  2:2388639/100‑1 (MQ=255)
                            ccTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCgg                                                                    <  2:2389596/100‑1 (MQ=255)
                            ccTGGGTGTGCTCTTTCAGCGCTTGTTCTTCGATCACACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACAAGAACAACGCgg                                                                    <  1:2422899/100‑1 (MQ=255)
                             cTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCgg                                                                    <  1:255329/99‑1 (MQ=255)
                             cTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGa                                                                   >  1:3981369/1‑100 (MQ=255)
                              tGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGAc                                                                  <  2:45707/100‑1 (MQ=255)
                                   gtTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACg                                                             <  1:3195236/100‑1 (MQ=255)
                                   gtTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACg                                                             >  1:630014/1‑100 (MQ=255)
                                     tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg                                                           <  2:3423229/100‑1 (MQ=255)
                                      ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga                                                          >  2:3113896/1‑100 (MQ=255)
                                      ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga                                                          >  1:4012461/1‑100 (MQ=255)
                                      ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga                                                          >  2:2781801/1‑100 (MQ=255)
                                      ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga                                                          <  2:3616062/100‑1 (MQ=255)
                                      ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTCTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga                                                          <  2:2888137/100‑1 (MQ=255)
                                        ctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgatg                                                        <  1:906796/100‑1 (MQ=255)
                                        ctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgatg                                                        <  1:1548931/100‑1 (MQ=255)
                                        ctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgatg                                                        >  2:457477/1‑100 (MQ=255)
                                          ttCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGAc                                                      <  1:1316076/100‑1 (MQ=255)
                                            cAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTg                                                    >  1:2751692/1‑100 (MQ=255)
                                            cAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGAGTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTg                                                    <  1:1991637/100‑1 (MQ=255)
                                             aGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTgg                                                   >  1:3089117/1‑100 (MQ=255)
                                             aGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTgg                                                   >  1:950603/1‑100 (MQ=255)
                                             aGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTgg                                                   >  2:3611538/1‑100 (MQ=255)
                                              gcgcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGa                                                  >  2:3573064/1‑100 (MQ=255)
                                               cgcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGan                                                 <  2:1772281/100‑2 (MQ=255)
                                               cgcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                 <  1:3289408/100‑1 (MQ=255)
                                                   tcttcttcGATCAGACGCAGAATAGAGAGCTACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGGCTGGATTCAc                                             >  1:2802430/1‑100 (MQ=255)
                                                   tcttcttcGATAAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTc                                               >  1:3278910/1‑98 (MQ=255)
                                                    cttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                            <  2:1086631/100‑1 (MQ=255)
                                                     ttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAccc                                           <  1:3686359/100‑1 (MQ=255)
                                                     ttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAccc                                           >  2:4013384/1‑100 (MQ=255)
                                                      tcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCa                                          >  1:1154829/1‑100 (MQ=255)
                                                      tcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCa                                          >  2:761136/1‑100 (MQ=255)
                                                           gATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGAt                                     >  2:1216223/1‑100 (MQ=255)
                                                            aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                    >  2:89556/1‑100 (MQ=255)
                                                            aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                    >  2:2776651/1‑100 (MQ=255)
                                                            aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                    >  2:3988331/1‑100 (MQ=255)
                                                            aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                    <  2:1581844/100‑1 (MQ=255)
                                                             tCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATgg                                   <  1:1657032/100‑1 (MQ=255)
                                                               aGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGc                                 <  2:3388805/100‑1 (MQ=255)
                                                               aGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGc                                 <  2:1376152/100‑1 (MQ=255)
                                                               aGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGc                                 <  2:1046014/100‑1 (MQ=255)
                                                                gACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCt                                >  1:3753488/1‑100 (MQ=255)
                                                                gACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCt                                <  1:4266552/100‑1 (MQ=255)
                                                                 aCGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTc                               <  2:4132255/100‑1 (MQ=255)
                                                                 aCGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTc                               >  1:2462166/1‑100 (MQ=255)
                                                                  cGCAGAATAGAGAGCGACAGCGACTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCa                              <  2:2133811/100‑1 (MQ=255)
                                                                   gCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAg                             <  2:655632/100‑1 (MQ=255)
                                                                    cAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGTGCTCAgg                            >  2:1855950/1‑100 (MQ=255)
                                                                    cAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg                            >  1:73359/1‑100 (MQ=255)
                                                                       aaTAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGgcgc                         <  2:1100090/100‑1 (MQ=255)
                                                                         tAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTg                       >  1:2867259/1‑100 (MQ=255)
                                                                           gagagCGACGGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGAc                     >  2:4218401/1‑100 (MQ=255)
                                                                           gagagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGAc                     >  2:2532664/1‑100 (MQ=255)
                                                                            agagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACg                    <  1:2959708/100‑1 (MQ=255)
                                                                             gagCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACgg                   <  2:1661441/100‑1 (MQ=255)
                                                                               gCGACAGCGATGCGTCGTGACGCACGGTGCCAGTGCCAGAACACCGGGGAGAAACGTTATGACTGGATTCGCCCATTGATGGGCTCAGGCGCTGACGGGa                 <  1:3395899/100‑1 (MQ=255)
                                                                                cGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGAc                <  1:3851716/100‑1 (MQ=255)
                                                                                 gACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACa               >  1:3172934/1‑100 (MQ=255)
                                                                                  aCAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAt              <  2:54656/100‑1 (MQ=255)
                                                                                  aCAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAt              >  2:2437935/1‑100 (MQ=255)
                                                                                  aCAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAt              >  1:1446265/1‑100 (MQ=255)
                                                                                     gCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTc           >  2:54562/1‑100 (MQ=255)
                                                                                     gCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTc           <  2:1179882/100‑1 (MQ=255)
                                                                                      cGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTcc          <  2:340587/100‑1 (MQ=255)
                                                                                        aTTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcag        >  1:2120413/1‑100 (MQ=255)
                                                                                        aTTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcag        >  1:1100647/1‑100 (MQ=255)
                                                                                         ttCGTTGTTACGCACGGTGTCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagc       >  1:3752523/1‑100 (MQ=255)
                                                                                         ttCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGGTTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagc       <  1:1294600/100‑1 (MQ=255)
                                                                                         ttCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagc       <  1:2256735/100‑1 (MQ=255)
                                                                                         ttCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagc       <  1:3491700/100‑1 (MQ=255)
                                                                                         ttCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagc       <  2:1807393/100‑1 (MQ=255)
                                                                                           cGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagcag     <  1:1375889/100‑1 (MQ=255)
                                                                                             ttGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAACCAGTGATGAGCTCAGGCGCTGACGGGACATTTCCAGCAGGc   >  1:2995439/1‑100 (MQ=255)
                                                                                              tGTTACGCACGGTGCCAGTACCAGAATAACGCGGACAAACGTTATGACTGTTTTCTCCCAGTGGTGGGCTCAAGCGCTGACGTGACATTTCCAGCAGGcc  <  1:1020030/100‑1 (MQ=255)
                                                                                                 |                                                                                                
ATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCC  >  NC_000913/1143028‑1143221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: