Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,224,157 A→G 40.8% intergenic (+250/+122) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,1570AG40.8% 76.9 / 160.6 142intergenic (+250/+122)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (50/34);  new base G (25/33);  total (75/67)
Fisher's exact test for biased strand distribution p-value = 6.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

CCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAG  >  NC_000913/1224058‑1224255
                                                                                                   |                                                                                                  
ccAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGa                                                                                                    <  2:2773020/100‑1 (MQ=255)
    tataTCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATa                                                                                                <  1:3110938/100‑1 (MQ=255)
    tataTCCATACTAACAATAAGGATATTTACTCAACCAGGCATAAACGATTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGa                                                                                                    <  1:2792426/96‑1 (MQ=255)
      tgtacaaaCTAAAAATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACGGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAg                                                                                               <  1:1540829/92‑1 (MQ=255)
      tatCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGc                                                                                              >  2:447894/1‑100 (MQ=255)
      tatCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGc                                                                                              <  2:3469696/100‑1 (MQ=255)
       atCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCa                                                                                             >  2:1930897/1‑100 (MQ=255)
       atCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCa                                                                                             >  1:2050187/1‑100 (MQ=255)
        tCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCaa                                                                                            <  2:1743229/100‑1 (MQ=255)
        tCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCa                                                                                             <  1:447980/99‑1 (MQ=255)
        tCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCaa                                                                                            >  2:3156478/1‑100 (MQ=255)
        tCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCaa                                                                                            >  1:3598386/1‑100 (MQ=255)
         ccATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAAt                                                                                           <  2:1453279/100‑1 (MQ=255)
         ccATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAAt                                                                                           <  2:3076567/100‑1 (MQ=255)
          cATACTAACAATAAGGTTATTTCCTCAACCAGGCATAAACACTTTGGTTTGCGCGTGGGAACAGCCTTAAGGTGTAACGGGGGAGGTGGAAATAGCAAt                                                                                           <  1:2090055/99‑1 (MQ=255)
           aTACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATga                                                                                         >  2:2509010/1‑100 (MQ=255)
            tACTAACAATAAGGTTATTTACTAAACCAGGCATAAACATTTTGTATGGTGCGTGGAAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATgag                                                                                        >  1:1672832/1‑100 (MQ=255)
             aCTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgagg                                                                                       >  1:1716843/1‑100 (MQ=255)
             aCTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgagg                                                                                       <  1:4083372/100‑1 (MQ=255)
             aCTAACAATAAGGTTATTTACGCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATgagg                                                                                       <  1:3158921/100‑1 (MQ=255)
              cTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATgagga                                                                                      >  1:184033/1‑100 (MQ=255)
               tAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATgaggag                                                                                     <  2:2092601/100‑1 (MQ=255)
                aaCAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGt                                                                                    >  1:539643/1‑100 (MQ=255)
                aaCAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGt                                                                                    >  2:2672205/1‑100 (MQ=255)
                aaCAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGt                                                                                    >  1:1881892/1‑100 (MQ=255)
                 aCAATAAAGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTCAAGGGGGAGGTGGGAATAGCAATGAGGAGTa                                                                                   <  2:608777/100‑1 (MQ=255)
                  cAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTTTTTGGGAACAGCCTTAAGGTGTAAAGGGGGATGTGGGAATAGCAATGAGGAGTAt                                                                                  >  1:630618/1‑100 (MQ=255)
                    aTAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCa                                                                                >  2:1295060/1‑100 (MQ=255)
                    aTAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCCATGAGGAGTATCa                                                                                <  1:195371/100‑1 (MQ=255)
                     tAAGGTTATTTACTCAACCAGGCATAAACATTTTGGTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAg                                                                               <  1:2585070/100‑1 (MQ=255)
                      aaGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGc                                                                              <  2:2566450/100‑1 (MQ=255)
                       aGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCa                                                                             >  2:4190151/1‑100 (MQ=255)
                       aGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCa                                                                             <  2:633365/100‑1 (MQ=255)
                       aGGTTATTTACTCAACCAGGCATAAAAATTTTGTTTTGTGCGTTGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAgta                                                                             <  1:2217680/100‑3 (MQ=255)
                         gTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAg                                                                           <  2:2159824/100‑1 (MQ=255)
                         gTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAg                                                                           <  2:3758198/100‑1 (MQ=255)
                         gTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAg                                                                           <  2:2863556/100‑1 (MQ=255)
                            aTTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAAt                                                                        <  1:4183831/100‑1 (MQ=255)
                                aCTGAACCAGGCATAAACATATTGTTTTGTGCGTGAGAACAGCCCTAAGGTGTAAAGAAGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTc                                                                    <  1:3537116/100‑1 (MQ=255)
                                aCTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTc                                                                    >  2:1712728/1‑100 (MQ=255)
                                aCTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTc                                                                    >  1:3365376/1‑100 (MQ=255)
                                aCTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTc                                                                    >  1:3458121/1‑100 (MQ=255)
                                   cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTcgcc                                                                 <  2:2820909/100‑1 (MQ=255)
                                   cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTcgcc                                                                 >  1:3649794/1‑100 (MQ=255)
                                    aaCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgccg                                                                <  1:1078681/100‑1 (MQ=255)
                                     aCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgccgc                                                               >  2:2064173/1‑100 (MQ=255)
                                         ggCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGGCGCTTTa                                                           <  1:2232494/100‑1 (MQ=255)
                                          gCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                                                          >  2:3482872/1‑100 (MQ=255)
                                           cATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAcc                                                         >  2:1759129/1‑100 (MQ=255)
                                           cATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCCATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                                                          >  1:3541151/1‑99 (MQ=255)
                                           cATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAcc                                                         <  1:3789590/100‑1 (MQ=255)
                                            aTAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACca                                                        <  2:376181/100‑1 (MQ=255)
                                              aaaCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACcaca                                                      <  1:698533/100‑1 (MQ=255)
                                                aCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAAc                                                    >  2:2836266/1‑100 (MQ=255)
                                                 cATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACg                                                   <  1:2672195/100‑1 (MQ=255)
                                                  aTTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGt                                                  >  1:3255835/1‑100 (MQ=255)
                                                   ttttgttttgTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTg                                                 >  2:3121010/1‑100 (MQ=255)
                                                    tttgttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTgg                                                >  2:3039134/1‑100 (MQ=255)
                                                      tgttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAt                                              >  2:1786129/1‑100 (MQ=255)
                                                       gttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATg                                             <  1:900518/100‑1 (MQ=255)
                                                         tttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATgag                                           >  1:154341/1‑100 (MQ=255)
                                                           tgtgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGagag                                         <  1:394711/100‑1 (MQ=255)
                                                            gtgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAgg                                        >  1:1502502/1‑100 (MQ=255)
                                                              gCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGa                                      <  2:2068452/100‑1 (MQ=255)
                                                               cGTGGGAACAGCCTTAAGGTGTAAAGGAGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTAGATGAAAGGGAt                                     >  1:1265328/1‑100 (MQ=255)
                                                                gTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATg                                    >  2:562532/1‑100 (MQ=255)
                                                                 tGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGa                                   >  1:1494658/1‑100 (MQ=255)
                                                                 tGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGa                                   >  1:3072309/1‑100 (MQ=255)
                                                                   ggAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGaaa                                 <  2:1494662/100‑1 (MQ=255)
                                                                    gAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGaaaa                                >  1:4140554/1‑100 (MQ=255)
                                                                     aaCAGCCTTAAGGTGTTAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGTCGCTTTACTACAACGTGGATGAGAGGGATGaaaaa                               >  1:845399/1‑100 (MQ=255)
                                                                      aCAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAAc                              <  2:2615622/100‑1 (MQ=255)
                                                                      aCAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAAc                              <  2:3838380/100‑1 (MQ=255)
                                                                      aCAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAAc                              <  2:4101749/100‑1 (MQ=255)
                                                                      aCAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAAc                              <  2:787927/100‑1 (MQ=255)
                                                                       cAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACt                             <  2:2253468/100‑1 (MQ=255)
                                                                          ccTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCaa                          >  2:193453/1‑100 (MQ=255)
                                                                           cTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAg                         <  2:1375549/100‑1 (MQ=255)
                                                                               aGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCag                     >  2:2485511/1‑100 (MQ=255)
                                                                                  tgtAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgaga                  >  2:251471/1‑100 (MQ=255)
                                                                                   gtAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt                 >  2:1378090/1‑100 (MQ=255)
                                                                                    tAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATa                >  1:1207433/1‑100 (MQ=255)
                                                                                    tAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATa                >  2:1140995/1‑100 (MQ=255)
                                                                                       aGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAct             >  2:3118065/1‑100 (MQ=255)
                                                                                        gggggAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAActc            <  2:2582292/100‑1 (MQ=255)
                                                                                        gggggAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAActc            <  2:2402078/100‑1 (MQ=255)
                                                                                          gggAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTg          <  2:3865233/100‑1 (MQ=255)
                                                                                          gggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTg          <  1:4124552/100‑1 (MQ=255)
                                                                                           ggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGc         >  1:1924693/1‑100 (MQ=255)
                                                                                             aGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCt       <  2:2939891/100‑1 (MQ=255)
                                                                                              ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCCTTACCACAACGTGGATGAGAGGGAAGAAAAACTCAAGGCAGAGATAACTCTGCCt       >  1:1564988/1‑99 (MQ=255)
                                                                                               gTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg     <  2:2090001/100‑1 (MQ=255)
                                                                                               gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg     <  2:2185567/100‑1 (MQ=255)
                                                                                               gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg     <  1:2064169/100‑1 (MQ=255)
                                                                                                tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGa    >  2:123681/1‑100 (MQ=255)
                                                                                                 ggAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa   >  2:452601/1‑100 (MQ=255)
                                                                                                 ggAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTCACCACAACGTTTATGAGAGGGATGAAAAACTCAAGGCAGTGATAACTCTGCCTTTaa   <  1:4204637/100‑1 (MQ=255)
                                                                                                  gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagt  <  1:1743653/100‑3 (MQ=255)
                                                                                                   aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagt  <  2:2279210/99‑3 (MQ=255)
                                                                                                   aaaTAGCAATGAGGAGTATCAGCAAGAATACTAGCCGCATTACCACAACGTGGATGAGAGAGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAg  >  1:3005817/1‑99 (MQ=255)
                                                                                                   |                                                                                                  
CCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAG  >  NC_000913/1224058‑1224255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: