BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	216,568	C→T	100%	A146T (GCC→ACC)	yaeF ←	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,568	0	C	T	98.8%	571.9 / ‑3.1	170	A146T (GCC→ACC)	yaeF	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (82/86); total (82/88)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 170 total aligned reads displayed.

GAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAAC  >  NC_000913/216473‑216664

gaACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAAc                                                                                              >  2:1954521/1‑100 (MQ=255)

gaACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAAc                                                                                              <  1:937345/100‑1 (MQ=255)

 aaCGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAAcg                                                                                             <  1:3141510/100‑1 (MQ=255)

  aCGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAAcgc                                                                                            <  1:1070929/100‑1 (MQ=255)

  aCGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAAcgc                                                                                            >  2:2025698/1‑100 (MQ=255)

    ggATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCgg                                                                                          >  1:3328460/1‑100 (MQ=255)

    ggATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCgg                                                                                          <  1:909968/100‑1 (MQ=255)

    ggATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCgg                                                                                          <  1:57867/100‑1 (MQ=255)

     gATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGt                                                                                         <  2:2468011/100‑1 (MQ=255)

      aTTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTg                                                                                        <  1:3555602/100‑1 (MQ=255)

      aTTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTg                                                                                        <  2:3907188/100‑1 (MQ=255)

      aTTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTg                                                                                        <  1:3796723/100‑1 (MQ=255)

       ttCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTg                                                                                        <  1:1313657/99‑1 (MQ=255)

        tCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGat                                                                                      >  1:1661243/1‑100 (MQ=255)

          aGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGatat                                                                                    >  2:3640630/1‑100 (MQ=255)

           gTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATc                                                                                   >  1:1252727/1‑100 (MQ=255)

           gTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATc                                                                                   >  2:3078639/1‑100 (MQ=255)

           gTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATc                                                                                   <  2:1932358/100‑1 (MQ=255)

            tGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATc                                                                                   <  1:3548240/99‑1 (MQ=255)

             gAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGCTTTTATTGGTAAACGCGGTGATATCtg                                                                                 >  2:636815/1‑100 (MQ=255)

              aGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCtgt                                                                                <  1:3078294/100‑1 (MQ=255)

               gCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCtgtg                                                                               <  1:3517740/100‑1 (MQ=255)

                  caTCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCt                                                                            >  2:1534025/1‑100 (MQ=255)

                  caTCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCt                                                                            >  1:1692991/1‑100 (MQ=255)

                   aTCTTGCGAGTCACCATAAAGGTAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTTGTAAACGCGGTGATATCtgtg                                                                               <  1:3817988/96‑1 (MQ=255)

                    tCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttn                                                                          <  1:4121302/100‑2 (MQ=255)

                       ggCGAGTGACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttgttg                                                                       <  2:641823/100‑1 (MQ=255)

                       ggCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttgttg                                                                       <  2:3346360/100‑1 (MQ=255)

                       ggCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttgt                                                                         >  1:2891051/1‑98 (MQ=255)

                       ggCGAGTCACCATAAAGGGAAGAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCttgttg                                                                       >  1:1500275/1‑100 (MQ=255)

                         cGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCg                                                                     <  2:3533907/100‑1 (MQ=255)

                            gTCACCATAAAGGTAATAACTGCGACAATGCCGCGATAGTTAGAGCCGCTATGTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCgggg                                                                  <  1:3683282/100‑1 (MQ=255)

                            gTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCgggg                                                                  >  1:3216109/1‑100 (MQ=255)

                            gTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCgggg                                                                  <  1:2675891/100‑1 (MQ=255)

                             tCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGt                                                                 <  1:335258/100‑1 (MQ=255)

                             tCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGt                                                                 <  1:1431216/100‑1 (MQ=255)

                              cACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTa                                                                >  1:1188863/1‑100 (MQ=255)

                              cACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTa                                                                >  2:307343/1‑100 (MQ=255)

                               aCCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTaa                                                               >  1:834791/1‑100 (MQ=255)

                                ccATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAg                                                              >  2:1725771/1‑100 (MQ=255)

                                 cATAAAGGGAGTAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGa                                                             >  1:2555150/1‑100 (MQ=255)

                                 cATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGa                                                             <  2:1293426/100‑1 (MQ=255)

                                      aGGGAACAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCgg                                                        <  1:2007566/100‑1 (MQ=255)

                                       gggAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCggg                                                       <  2:3350517/100‑1 (MQ=255)

                                       gggAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCggg                                                       <  2:137704/100‑1 (MQ=255)

                                       gggAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCggg                                                       <  1:303790/100‑1 (MQ=255)

                                       gggAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCggg                                                       <  1:1935328/100‑1 (MQ=255)

                                       gggAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCggg                                                       <  2:1252730/100‑1 (MQ=255)

                                         gAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGAc                                                     >  2:2564961/1‑100 (MQ=255)

                                           aTAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTc                                                   >  2:1884958/1‑100 (MQ=255)

                                             aaaTTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGt                                                 >  1:1864305/1‑100 (MQ=255)

                                              aaTTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTa                                                >  2:2540369/1‑100 (MQ=255)

                                              aaTTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTa                                                >  1:644076/1‑100 (MQ=255)

                                              aaTTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTa                                                <  2:2700583/100‑1 (MQ=255)

                                              aaTTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTa                                                <  2:694796/100‑1 (MQ=255)

                                               aTTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTaa                                               >  1:2870807/1‑100 (MQ=255)

                                               aTTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTa                                                >  1:2768166/1‑99 (MQ=255)

                                                ttCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAg                                              <  2:3647170/100‑1 (MQ=255)

                                                ttCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAg                                              <  2:1119897/100‑1 (MQ=255)

                                                 tCGACAATGCCGCGATAATTATAACCGCTATCTGTGATTTTACTGGTAAACGCGGGGATATCGTTTGCTTATTGCGGGGTACGCTACGGGACTCGTAAg                                              >  1:408982/1‑99 (MQ=255)

                                                  cGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGAc                                            <  1:208279/100‑1 (MQ=255)

                                                     cAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTACACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGCCTCGTAAGACg                                           >  2:2488025/1‑98 (MQ=255)

                                                      aaTGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGCAAGATCCGGGACTCGTAAGACGaaa                                        >  1:2210721/1‑100 (MQ=255)

                                                       aggCCGCGATAGTAATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGaaaa                                       >  2:2897326/3‑100 (MQ=255)

                                                        tggcgggATGGTTGTAACCGCTATCTTGGATTTTATTGGTAAACGCGGTGATATCTGTGGGTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAg                                      <  1:1969770/94‑1 (MQ=255)

                                                        tGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAg                                      <  1:3772196/100‑1 (MQ=255)

                                                          ccGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCt                                    >  2:1016265/1‑100 (MQ=255)

                                                          ccGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCt                                    >  2:461238/1‑100 (MQ=255)

                                                          ccGCGATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCt                                    <  2:2934612/100‑1 (MQ=255)

                                                            gcgATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTa                                  <  1:82486/100‑1 (MQ=255)

                                                            gcgATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTa                                  >  2:4221005/1‑100 (MQ=255)

                                                             cgATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTAt                                 <  2:606926/100‑1 (MQ=255)

                                                             cgATAGTTATAACCGCTATCTTTGATTTTATTGCTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTa                                  <  1:2253687/99‑1 (MQ=255)

                                                              gATAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATc                                <  1:463276/100‑1 (MQ=255)

                                                                tAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCTTAAGACTAAAAGCTTTTCAc                              >  1:3623234/1‑100 (MQ=255)

                                                                tAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCAc                              <  2:2561691/100‑1 (MQ=255)

                                                                tAGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCAc                              <  1:1836295/100‑1 (MQ=255)

                                                                 aGTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACt                             <  2:2407379/100‑1 (MQ=255)

                                                                  gTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTa                            >  2:808089/1‑100 (MQ=255)

                                                                  gTTATAACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTa                            >  2:531955/1‑100 (MQ=255)

                                                                     ataACCGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGc                         <  2:3404036/100‑1 (MQ=255)

                                                                     ataACCGCTATCTTTGATTTTATTGGTAAACGAGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGc                         >  2:4049263/1‑100 (MQ=255)

                                                                          cGCTATCTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCAt                    <  2:159434/100‑1 (MQ=255)

                                                                           gCTATCTTTGATTTTTTTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATc                   <  2:4206381/100‑1 (MQ=255)

                                                                                cTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCttt              <  2:2860819/100‑1 (MQ=255)

                                                                                cTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCttt              >  1:3114637/1‑100 (MQ=255)

                                                                                cTTTGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCttt              <  1:4087293/100‑1 (MQ=255)

                                                                                cTTTGATTTTATTGGTAAACGCGCTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGc                 <  1:2644838/97‑1 (MQ=255)

                                                                                 tttGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCtttt             >  2:2241953/1‑100 (MQ=255)

                                                                                  ttGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCttttt            <  1:3461194/100‑1 (MQ=255)

                                                                                   tGATTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCtttttt           <  2:2888999/100‑1 (MQ=255)

                                                                                     aTTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTaa         >  1:433542/1‑100 (MQ=255)

                                                                                     aTTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTaa         <  1:3524813/100‑1 (MQ=255)

                                                                                     aTTTTATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTaa         >  1:3503173/1‑100 (MQ=255)

                                                                                       tttATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAgg       <  1:221389/100‑1 (MQ=255)

                                                                                       tttATTGGTAAACGCGGTGATATCGGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACAAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAgg       <  1:9468/100‑1 (MQ=255)

                                                                                        ttATTGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAggg      >  2:2007573/1‑100 (MQ=255)

                                                                                          aTTGGTAAACGCGGAGATATATGTGGCTTGTGGCGGGGTAAGATCCGGGAAAAAAAAAAAAAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAgg       <  1:1553099/97‑1 (MQ=255)

                                                                                           ttGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAATGCTTATCACTATGCTTCATCGCTTTTTTAAGGGaaa   >  2:4153400/1‑100 (MQ=255)

                                                                                            tGGTAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAAc  <  1:1104359/100‑1 (MQ=255)

GAACGGATTCAGTGAGCACATCTGGCGAGTCACCATAAAGGGAATAAATTCGACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAAC  >  NC_000913/216473‑216664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`