Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,232,481 C→T 15.0% intergenic (+27/+19) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,232,4810CT15.0% 404.5 / 50.6 165intergenic (+27/+19)umuC/dsbBtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base C (73/67);  new base T (10/15);  total (83/82)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

Only 100 of 170 total aligned reads displayed.

GGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGAT  >  NC_000913/1232384‑1232580
                                                                                                 |                                                                                                   
ggCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGAAAAAAAAAAAAAGCGCTCCCGc                                                                                                   <  1:291564/100‑1 (MQ=255)
  cAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGACAAAAGCGCCCCCGc                                                                                                   >  1:3491551/1‑98 (MQ=255)
   aGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAgg                                                                                                <  2:2632393/100‑1 (MQ=255)
         cgctagacATGCTTTCACCACGTTATCCAACGCGAAGTGCTGCTTTACTAAGGGTCAACTAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgct                                                                                          <  1:1476754/93‑2 (MQ=255)
         aGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgct                                                                                          <  2:1083365/100‑2 (MQ=255)
         aGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                          >  2:2973100/1‑100 (MQ=255)
          gCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCg                                                                                         <  2:340358/100‑1 (MQ=255)
           cGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt                                                                                        <  1:301348/100‑4 (MQ=255)
            gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                       <  2:1673895/100‑5 (MQ=255)
            gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCGGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGa                                                                                        >  1:856416/1‑99 (MQ=255)
             aGCCATGTTTGCGCCACGTTATACAACTTTAATTTCTTTTTGACTGAGGGTCAAATAAATATAAAAACAACAAAAAAGCGCTCCCGCAGGAGCGCCGAAt                                                                                      <  1:2202254/100‑1 (MQ=255)
             aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttt                                                                                      <  2:977536/100‑6 (MQ=255)
             aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttt                                                                                      <  1:897816/100‑6 (MQ=255)
             aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttt                                                                                      <  1:1638477/100‑6 (MQ=255)
             aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAAt                                                                                      <  1:267563/100‑1 (MQ=255)
              gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttttt                                                                                     <  2:2764727/100‑7 (MQ=255)
              gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttt                                                                                      <  2:4170315/99‑6 (MQ=255)
               ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATgg                                                                                    <  1:2706839/100‑1 (MQ=255)
                cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                   >  1:451153/1‑100 (MQ=255)
                 aTGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcc                                                                                   <  1:3920808/99‑9 (MQ=38)
                  tGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGAtt                                                                                 >  2:514940/1‑100 (MQ=255)
                    cTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAg                                                                               <  2:1184822/100‑1 (MQ=255)
                     tttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGc                                                                              <  1:1691485/100‑1 (MQ=255)
                      ttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCg                                                                             >  2:191855/1‑100 (MQ=255)
                        caccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAc                                                                           >  1:374807/1‑100 (MQ=255)
                         accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                          <  2:2369620/100‑1 (MQ=255)
                          ccacTTTATACAACGCGAAGTTCTTATTCACTGAGCGTCAAATAAATATATCGGCAGGAAAAATTCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                          <  1:408868/99‑1 (MQ=255)
                          ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                          <  1:2533229/99‑1 (MQ=255)
                           cacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTAAAATAAATATATCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGa                                                                        <  1:625939/100‑1 (MQ=255)
                             cGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAAc                                                                      >  1:746167/1‑100 (MQ=255)
                                  taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATc                                                                 >  1:4255670/1‑100 (MQ=255)
                                   aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCa                                                                >  1:99541/1‑100 (MQ=255)
                                      aCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGt                                                             >  2:3739163/1‑100 (MQ=255)
                                         cgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGtttt                                                          <  1:2071397/100‑1 (MQ=255)
                                            aGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                       <  2:3317428/100‑1 (MQ=255)
                                             gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCg                                                      >  2:3577901/1‑100 (MQ=255)
                                             gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCg                                                      <  1:3990918/100‑1 (MQ=255)
                                              ttCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGc                                                     <  2:1339462/100‑1 (MQ=255)
                                              ttCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGc                                                     <  1:1922100/100‑1 (MQ=255)
                                               tCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCt                                                    <  1:2780633/100‑1 (MQ=255)
                                                cTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAACAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCtt                                                   <  2:3609286/100‑1 (MQ=255)
                                                 tGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCttt                                                  <  2:1844295/100‑1 (MQ=255)
                                                  gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTa                                                 >  1:1973844/1‑100 (MQ=255)
                                                  gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTa                                                 <  2:4255693/100‑1 (MQ=255)
                                                  gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTa                                                 >  2:3023620/1‑100 (MQ=255)
                                                  gATATACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGACGAATGGATTAGCGACCGAACAGATCACGATTTTTCGCTTTa                                                 >  1:204299/1‑100 (MQ=255)
                                                      tACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAAc                                              >  1:3876080/1‑99 (MQ=255)
                                                      tACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACg                                             <  1:1448515/100‑1 (MQ=255)
                                                        cTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACCGATCACGTTTTTTCTCttt                                                  >  1:503675/1‑93 (MQ=255)
                                                          gAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTg                                         <  1:3176703/100‑1 (MQ=255)
                                                            gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                       <  1:2169204/100‑1 (MQ=255)
                                                             ggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGa                                      >  2:1437120/1‑100 (MQ=255)
                                                             ggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGa                                      >  1:1964371/1‑100 (MQ=255)
                                                             gctCAAATAAAAATAGCGGCAGGAAAAACGCGCGCCCGCAGGAGCGCCGAAGGGATTAGGGACCGAACTGACCTCGTTTTTTCGCTTTAAACGTCTGGGa                                      >  1:1685006/3‑100 (MQ=255)
                                                              gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaa                                     >  2:4116458/1‑100 (MQ=255)
                                                              gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGCTTTTTCGCTTTAAACGGCTGGGaa                                     >  1:3251349/1‑100 (MQ=255)
                                                                cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGAACACGTTTTTTCGCTTTAAACGGCTGGGAAAt                                   >  1:3326788/1‑100 (MQ=255)
                                                                  nataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATca                                 >  2:2289255/2‑100 (MQ=255)
                                                                  aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATca                                 >  2:2398572/1‑100 (MQ=255)
                                                                  aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATca                                 >  1:2892340/1‑100 (MQ=255)
                                                                   ataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcac                                <  1:1216007/100‑1 (MQ=255)
                                                                    taaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcacc                               <  2:1984064/100‑1 (MQ=255)
                                                                      aataTAGCTGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACATATCACCTTTTTTCGCTTTAAACGGCTGGGAAATcaccac                             <  1:2558056/100‑1 (MQ=255)
                                                                       atatAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCaccacc                            <  1:881385/100‑1 (MQ=255)
                                                                       atatAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCaccacc                            <  2:234053/100‑1 (MQ=255)
                                                                        tataGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGTCTGGGAAATCAccacca                           >  2:1557424/1‑100 (MQ=255)
                                                                        tataGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAccacca                           >  1:1778714/1‑100 (MQ=255)
                                                                        tataGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAccacca                           <  1:2398566/100‑1 (MQ=255)
                                                                         ataGCGGCAGGAAAAAAACGCGCCCGCAGGATATCCGAATGGATTATCGACCGAACAGATCACGTGTTTCCGCTTTAAACGGATAGGAAATCacaacc                            >  1:3860119/1‑98 (MQ=255)
                                                                          taGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTATCGACCTAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGc                         <  1:3780519/100‑1 (MQ=255)
                                                                          taGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGc                         >  2:1710435/1‑100 (MQ=255)
                                                                          taGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGc                         >  2:3995365/1‑100 (MQ=255)
                                                                           aGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCa                        >  2:3944535/1‑100 (MQ=255)
                                                                             cGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACt                      <  2:257934/100‑1 (MQ=255)
                                                                              ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTg                     >  1:4153747/1‑100 (MQ=255)
                                                                               gCAGGAAAAAAGCGCTCCCGCAGGATCGCCGAATGGGTTACCGACCGAACAGATCACGTTTTTTCGCTGTAAACGGCTGGGAAATCACCACCAGCACTGc                    <  1:3626793/100‑1 (MQ=255)
                                                                               gCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGc                    >  2:701191/1‑100 (MQ=255)
                                                                                  ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGAc                 <  2:1427471/100‑1 (MQ=255)
                                                                                   gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGATGGGACATCACCACCAACACGGc                    >  1:286508/1‑96 (MQ=255)
                                                                                   ccattcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTTTTTTTTTTTGCCTGGAAAAACACCACCAGCACTTCGAc                 >  1:3391991/8‑99 (MQ=16)
                                                                                    cattcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACaa               >  1:1934630/7‑100 (MQ=255)
                                                                                    cattcggcgcTCCTGCGGGAGCACCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACaa               >  1:2477269/7‑100 (MQ=255)
                                                                                    aaaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGCAATCACCACCAGCACTGCGACaa               >  1:1284725/1‑100 (MQ=255)
                                                                                    aaaaaaGCGCGCCCGCAGGAGCGCCGAATTAATTAGCGACCGAACAGATCTCGTTTTTTCGTTTTAAACGGTTGGGAAATCACCACCAGCACTGCg                   <  1:835986/96‑1 (MQ=255)
                                                                                     attcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt              >  2:90534/6‑100 (MQ=255)
                                                                                     aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt              >  2:619697/1‑100 (MQ=255)
                                                                                     aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt              <  1:4048332/100‑1 (MQ=255)
                                                                                      ttcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCAAGTTTTTTCGCTTTAAACGGCTGGGCAATCACCACCAGCACTGCGACAATc             >  1:3608891/5‑100 (MQ=255)
                                                                                      aaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc             <  2:803077/100‑1 (MQ=255)
                                                                                       aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa            <  1:2044630/100‑1 (MQ=255)
                                                                                       aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa            <  1:800516/100‑1 (MQ=255)
                                                                                        cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg           >  2:3499352/3‑100 (MQ=255)
                                                                                        cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg           >  2:308649/3‑100 (MQ=255)
                                                                                        cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg           >  2:2091493/3‑100 (MQ=255)
                                                                                         aGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTCAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg          <  1:1038832/100‑1 (MQ=255)
                                                                                          gcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGt         >  2:3438334/1‑100 (MQ=255)
                                                                                          gcgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGt         <  2:1309873/100‑1 (MQ=255)
                                                                                           cgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTa        >  1:3090863/1‑100 (MQ=255)
                                                                                                 cGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTCAGCGAt  >  1:3084673/1‑100 (MQ=255)
                                                                                                 cGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGAt  <  1:4144791/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
GGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGAT  >  NC_000913/1232384‑1232580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: